Restriction map for
pFN21AB8462
(Circular) (Six-base) MAPSORT of: pFN21AB8462.seq Check: 6474 from: 1 to: 5833
pFN21AB8462 5833 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3643 278
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3176 1937
Size: 1239 4594
Acc65I G'GTAC_C
Cuts at: 2857 3154 2857
Size: 297 5536
AclI AA'CG_TT
Cuts at: 1563 3960 4333 1563
Size: 2397 373 3063
Fragments arranged by size:
3063 2397 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3896 5280 5548 1632
Size: 2264 1384 268 1917
Fragments arranged by size:
2264 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5636
AflIII A'CryG_T
Cuts at: 1203 2566 4732 5709 1203
Size: 1363 2166 977 1327
Fragments arranged by size:
2166 1363 1327 977
AgeI A'CCGG_T
Cuts at: 2165 4664 2165
Size: 2499 3334
AhdI GACnn_n'nnGTC
Cuts at: 4562 4562
Size: 5833
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5833
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5833
AlwNI CAG_nnn'CTG
Cuts at: 2996 3576 5148 2996
Size: 580 1572 3681
Fragments arranged by size:
3681 1572 580
ApaLI G'TGCA_C
Cuts at: 3890 5046 3890
Size: 1156 4677
ApoI r'AATT_y
Cuts at: 1493 2115 2311 3142 3368 3520 5629 1493
Size: 622 196 831 226 152 2109 1697
Fragments arranged by size:
2109 1697 831 622 226 196 152
AseI AT'TA_AT
Cuts at: 160 1032 4387 160
Size: 872 3355 1606
Fragments arranged by size:
3355 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
AvaI C'yCGr_G
Cuts at: 1870 1943 3158 1870
Size: 73 1215 4545
Fragments arranged by size:
4545 1215 73
AvrII C'CTAG_G
Cuts at: 2689 2689
Size: 5833
BamHI G'GATC_C
Cuts at: 3163 3530 3163
Size: 367 5466
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2134 2519 2857 3154 4609
Size: 325 217 627 347 385 338 297 1455
Cuts at: 4609 618
Size: 1842
Fragments arranged by size:
1842 1455 627 385 347 338 325 297
217
BanII G_rGCy'C
Cuts at: 1391 2812 3152 1391
Size: 1421 340 4072
Fragments arranged by size:
4072 1421 340
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4862
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2323 2357 2465 2499 4024 4058 1903
Size: 34 386 34 108 34 1525 34 3678
Fragments arranged by size:
3678 1525 386 108 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3725 4935 3725
Size: 1210 4623
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5833
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2789 4444 136
Size: 107 122 71 631 605 1117 1655 1525
Fragments arranged by size:
1655 1525 1117 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5828 5828
Size: 5833
BlpI GC'TnA_GC
Cuts at: 3250 3250
Size: 5833
Bme1580I G_kGCm'C
Cuts at: 1790 2712 3894 5050 1790
Size: 922 1182 1156 2573
Fragments arranged by size:
2573 1182 1156 922
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5833
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2618 3564 4522
Size: 416 53 83 399 24 1201 946 958
Cuts at: 4522 442
Size: 1753
Fragments arranged by size:
1753 1201 958 946 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3072 1056
Size: 2016 3817
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4493 1239
Size: 3254 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2130 3958 4823 5121 2130
Size: 1828 865 298 2842
Fragments arranged by size:
2842 1828 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4496 882
Size: 617 2997 2219
Fragments arranged by size:
2997 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2567 3624 493
Size: 711 1363 1057 2702
Fragments arranged by size:
2702 1363 1057 711
BsaBI GATnn'nnATC
Cuts at: 5827 5827
Size: 5833
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3640 4022 4657 275
Size: 53 83 186 936 2107 382 635 1451
Fragments arranged by size:
2107 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2165 2647 4266 4664 4938 5085 5496 2165
Size: 482 1619 398 274 147 411 2502
Fragments arranged by size:
2502 1619 482 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2038 2068 1178
Size: 30 165 30 635 30 4943
Fragments arranged by size:
4943 635 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2820 1186
Size: 1634 4199
BseYI C'CCAG_C
Cuts at: 2231 5036 5532 2231
Size: 2805 496 2532
Fragments arranged by size:
2805 2532 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2865 1163
Size: 573 1129 4131
Fragments arranged by size:
4131 1129 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3283 4044 5072 1540
Size: 275 186 1282 761 1028 2301
Fragments arranged by size:
2301 1282 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2272 3894 3979 5050 2272
Size: 1622 85 1071 3055
Fragments arranged by size:
3055 1622 1071 85
BsmI GAATG_Cn'
Cuts at: 3344 3437 3344
Size: 93 5740
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2272 2712 2812 3152 3894 3979 5050
Size: 399 482 440 100 340 742 85 1071
Cuts at: 5050 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 482 440 399 340 100
85
BspEI T'CCGG_A
Cuts at: 2647 5496 2647
Size: 2849 2984
BspHI T'CATG_A
Cuts at: 3722 5452 3722
Size: 1730 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3188 844
Size: 2344 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2855 3720 1394
Size: 429 1032 865 3507
Fragments arranged by size:
3507 1032 865 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2070 2701 4328 4502 65
Size: 1183 822 631 1627 174 1396
Fragments arranged by size:
1627 1396 1183 822 631 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2156 2165 4477 4664 1700
Size: 110 346 9 2312 187 2869
Fragments arranged by size:
2869 2312 346 187 110 9
BsrGI T'GTAC_A
Cuts at: 96 3030 96
Size: 2934 2899
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5833
BssSI C'ACGA_G
Cuts at: 3893 4905 3893
Size: 1012 4821
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2960 2996 794
Size: 2166 36 3631
Fragments arranged by size:
3631 2166 36
BstBI TT'CG_AA
Cuts at: 4637 4637
Size: 5833
BstEII G'GTnAC_C
Cuts at: 1184 2033 1184
Size: 849 4984
BstYI r'GATC_y
Cuts at: 1972 2246 3163 3530 3915 3932 5384 5470 5482
Size: 274 917 367 385 17 1452 86 12
Cuts at: 5482 5828 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 917 385 367 346 274 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2206 2523 2787 513
Size: 552 939 202 317 264 3559
Fragments arranged by size:
3559 939 552 317 264 202
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5094
Fragments arranged by size:
5094 683 56
BtsI GCAGTG_nn'
Cuts at: 2712 3349 4143 4163 2712
Size: 637 794 20 4382
Fragments arranged by size:
4382 794 637 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5833
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5798
DraI TTT'AAA
Cuts at: 3137 3137
Size: 5833
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4840 809
Size: 507 3524 1802
Fragments arranged by size:
3524 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2158 2281 3280
Size: 54 1425 50 275 18 328 123 999
Cuts at: 3280 4169 5798 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 999 889 328 275 123 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3280 1537
Size: 275 1468 4090
Fragments arranged by size:
4090 1468 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2716 3763 1353
Size: 1363 1047 3423
Fragments arranged by size:
3423 1363 1047
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2845 4420 4794 4940 387
Size: 1064 138 1256 1575 374 146 1280
Fragments arranged by size:
1575 1280 1256 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3896 4493 5280 5548 1239
Size: 393 2264 597 787 268 1524
Fragments arranged by size:
2264 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2374 2374
Size: 5833
EcoRI G'AATT_C
Cuts at: 2115 3142 3520 2115
Size: 1027 378 4428
Fragments arranged by size:
4428 1027 378
FspI TGC'GCA
Cuts at: 4339 4339
Size: 5833
HaeII r_GCGC'y
Cuts at: 1678 3087 4980 1678
Size: 1409 1893 2531
Fragments arranged by size:
2531 1893 1409
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2038 2070 2309 2341 2715
Size: 32 135 32 466 32 239 32 374
Cuts at: 2715 2747 4474 4506 4548 4580 1373
Size: 32 1727 32 42 32 2626
Fragments arranged by size:
2626 1727 466 374 239 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3177 3423 669
Size: 988 281 1239 246 3079
Fragments arranged by size:
3079 1239 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5833
HpaI GTT'AAC
Cuts at: 3423 3423
Size: 5833
KpnI G_GTAC'C
Cuts at: 2861 3158 2861
Size: 297 5536
MfeI C'AATT_G
Cuts at: 3432 3432
Size: 5833
MluI A'CGCG_T
Cuts at: 5709 5709
Size: 5833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2591 2611 2888 4947 5131 1305
Size: 1286 20 277 2059 184 2007
Fragments arranged by size:
2059 2007 1286 277 184 20
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4011
Fragments arranged by size:
4011 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3791 4150 4309 518
Size: 648 2625 359 159 2042
Fragments arranged by size:
2625 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2984 2996 3249 3926 5074 5319 1606
Size: 1378 12 253 677 1148 245 2120
Fragments arranged by size:
2120 1378 1148 677 253 245 12
NaeI GCC'GGC
Cuts at: 2158 2158
Size: 5833
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4342
Fragments arranged by size:
4342 939 552
NdeI CA'TA_TG
Cuts at: 387 2599 387
Size: 2212 3621
NgoMIV G'CCGG_C
Cuts at: 2156 2156
Size: 5833
NheI G'CTAG_C
Cuts at: 1052 3068 1052
Size: 2016 3817
NotI GC'GGCC_GC
Cuts at: 3280 3280
Size: 5833
NspI r_CATG'y
Cuts at: 1132 1229 2348 3191 4736 1132
Size: 97 1119 843 1545 2229
Fragments arranged by size:
2229 1545 1119 843 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5833
PciI A'CATG_T
Cuts at: 4732 4732
Size: 5833
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2630 1108
Size: 1522 4311
PfoI T'CCnGG_A
Cuts at: 2370 2370
Size: 5833
PmeI GTTT'AAAC
Cuts at: 3137 3137
Size: 5833
PmlI CAC'GTG
Cuts at: 2567 2567
Size: 5833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3902 3934 5415 5447 3902
Size: 32 1481 32 4288
Fragments arranged by size:
4288 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2374 2374
Size: 5833
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5833
PsiI TTA'TAA
Cuts at: 3403 3403
Size: 5833
PstI C_TGCA'G
Cuts at: 830 2782 2992 3001 3185 830
Size: 1952 210 9 184 3478
Fragments arranged by size:
3478 1952 210 184 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5833
SalI G'TCGA_C
Cuts at: 1936 3175 1936
Size: 1239 4594
SanDI GG'GwC_CC
Cuts at: 2374 2374
Size: 5833
SbfI CC_TGCA'GG
Cuts at: 3185 3185
Size: 5833
ScaI AGT'ACT
Cuts at: 4081 5793 4081
Size: 1712 4121
SfcI C'TryA_G
Cuts at: 826 1045 2559 2778 2944 2988 2997 3112 3181
Size: 219 1514 219 166 44 9 115 69
Cuts at: 3181 4316 4997 5188 826
Size: 1135 681 191 1471
Fragments arranged by size:
1514 1471 1135 681 219 219 191 166
115 69 44 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5833
SmaI CCC'GGG
Cuts at: 1872 3160 1872
Size: 1288 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2145 3937 4838 5100 820
Size: 197 926 202 1792 901 262 1553
Fragments arranged by size:
1792 1553 926 901 262 202 197
SnaBI TAC'GTA
Cuts at: 493 3624 493
Size: 3131 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5833
SphI G_CATG'C
Cuts at: 1132 3191 1132
Size: 2059 3774
SspI AAT'ATT
Cuts at: 5 52 2356 3757 5
Size: 47 2304 1401 2081
Fragments arranged by size:
2304 2081 1401 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5833
StyI C'CwwG_G
Cuts at: 513 1065 2004 2689 3272 513
Size: 552 939 685 583 3074
Fragments arranged by size:
3074 939 685 583 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2245 3860 3877 4030 4215 4660 325
Size: 1255 665 1615 17 153 185 445 1498
Fragments arranged by size:
1615 1498 1255 665 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3030 4079 5791
Size: 274 80 33 51 1443 1053 1049 1712
Cuts at: 5791 96
Size: 138
Fragments arranged by size:
1712 1443 1053 1049 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2728 2760 5793 5825 2728
Size: 32 3033 32 2736
Fragments arranged by size:
3033 2736 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5833
XbaI T'CTAG_A
Cuts at: 3169 3169
Size: 5833
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2584 2584
Size: 5833
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5833
XmaI C'CCGG_G
Cuts at: 1870 3158 1870
Size: 1288 4545
XmnI GAAnn'nnTTC
Cuts at: 3962 3962
Size: 5833
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3641 276
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI
SwaI