Restriction map for
pFN21AB8461
(Circular) (Six-base) MAPSORT of: pFN21AB8461.seq Check: 8434 from: 1 to: 5281
pFN21AB8461 5281 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2235 3091 278
Size: 53 83 186 936 699 856 2468
Fragments arranged by size:
2468 936 856 699 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2624 1937
Size: 687 4594
Acc65I G'GTAC_C
Cuts at: 2602 2602
Size: 5281
AclI AA'CG_TT
Cuts at: 1563 3408 3781 1563
Size: 1845 373 3063
Fragments arranged by size:
3063 1845 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2411 3344 4728 4996 1632
Size: 779 933 1384 268 1917
Fragments arranged by size:
1917 1384 933 779 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5084
AflIII A'CryG_T
Cuts at: 1203 4180 5157 1203
Size: 2977 977 1327
Fragments arranged by size:
2977 1327 977
AgeI A'CCGG_T
Cuts at: 4112 4112
Size: 5281
AhdI GACnn_n'nnGTC
Cuts at: 4010 4010
Size: 5281
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5281
AlwNI CAG_nnn'CTG
Cuts at: 2431 3024 4596 2431
Size: 593 1572 3116
Fragments arranged by size:
3116 1572 593
ApaLI G'TGCA_C
Cuts at: 3338 4494 3338
Size: 1156 4125
ApoI r'AATT_y
Cuts at: 1493 2590 2816 2968 5077 1493
Size: 1097 226 152 2109 1697
Fragments arranged by size:
2109 1697 1097 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3835 160
Size: 872 2803 1606
Fragments arranged by size:
2803 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5281
AvaI C'yCGr_G
Cuts at: 1870 1943 2606 1870
Size: 73 663 4545
Fragments arranged by size:
4545 663 73
BamHI G'GATC_C
Cuts at: 2611 2978 2611
Size: 367 4914
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2310 2345 2602 4057 618
Size: 325 217 627 523 35 257 1455 1842
Fragments arranged by size:
1842 1455 627 523 325 257 217 35
BanII G_rGCy'C
Cuts at: 1391 2600 1391
Size: 1209 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2227 928
Size: 971 328 3982
Fragments arranged by size:
3982 971 328
BbvCI CC'TCA_GC
Cuts at: 2541 2541
Size: 5281
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2560 2594 3472 3506 1903
Size: 34 50 34 539 34 878 34 3678
Fragments arranged by size:
3678 878 539 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3173 4383 3173
Size: 1210 4071
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5281
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2525 2551
Size: 107 122 71 631 605 337 516 26
Cuts at: 2551 3892 136
Size: 1341 1525
Fragments arranged by size:
1525 1341 631 605 516 337 122 107
71 26
BglII A'GATC_T
Cuts at: 5276 5276
Size: 5281
BlpI GC'TnA_GC
Cuts at: 2476 2698 2476
Size: 222 5059
Bme1580I G_kGCm'C
Cuts at: 1790 2348 2540 3342 4498 1790
Size: 558 192 802 1156 2573
Fragments arranged by size:
2573 1156 802 558 192
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5281
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3012 3970 442
Size: 416 53 83 399 24 1595 958 1753
Fragments arranged by size:
1753 1595 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5281
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2470 2470
Size: 5281
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2489 3941 1239
Size: 1250 1452 2579
Fragments arranged by size:
2579 1452 1250
Bpu10I CC'TnA_GC
Cuts at: 2541 2541
Size: 5281
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3406 4271 4569 3406
Size: 865 298 4118
Fragments arranged by size:
4118 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3944 882
Size: 617 2445 2219
Fragments arranged by size:
2445 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3072 493
Size: 711 1868 2702
Fragments arranged by size:
2702 1868 711
BsaBI GATnn'nnATC
Cuts at: 5275 5275
Size: 5281
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2232 3088 3470 4105
Size: 53 83 186 936 699 856 382 635
Cuts at: 4105 275
Size: 1451
Fragments arranged by size:
1451 936 856 699 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3714 4112 4386 4533 4944 3714
Size: 398 274 147 411 4051
Fragments arranged by size:
4051 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5056
Fragments arranged by size:
5056 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2567 1186
Size: 1381 3900
BseYI C'CCAG_C
Cuts at: 2180 2263 2321 4484 4980 2180
Size: 83 58 2163 496 2481
Fragments arranged by size:
2481 2163 496 83 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4708
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2200 2210 2731 3492 4520 1540
Size: 275 186 199 10 521 761 1028 2301
Fragments arranged by size:
2301 1028 761 521 275 199 186 10
BsiHKAI G_wGCw'C
Cuts at: 2477 3342 3427 4498 2477
Size: 865 85 1071 3260
Fragments arranged by size:
3260 1071 865 85
BsmI GAATG_Cn'
Cuts at: 2792 2885 2792
Size: 93 5188
BsmBI CGTCTCn'nnnn_
Cuts at: 2207 2207
Size: 5281
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2348 2477 2540 2600 3342 3427 4498
Size: 399 558 129 63 60 742 85 1071
Cuts at: 4498 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 558 399 129 85 63
60
BspEI T'CCGG_A
Cuts at: 4944 4944
Size: 5281
BspHI T'CATG_A
Cuts at: 3170 4900 3170
Size: 1730 3551
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2063 2367 2636 844
Size: 1219 304 269 3489
Fragments arranged by size:
3489 1219 304 269
BsrBI CCG'CTC
Cuts at: 1394 1823 2212 3168 1394
Size: 429 389 956 3507
Fragments arranged by size:
3507 956 429 389
BsrDI GCAATG_nn'
Cuts at: 65 1248 3776 3950 65
Size: 1183 2528 174 1396
Fragments arranged by size:
2528 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3925 4112 1700
Size: 110 2115 187 2869
Fragments arranged by size:
2869 2115 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5281
BssHII G'CGCG_C
Cuts at: 1925 2563 1925
Size: 638 4643
BssSI C'ACGA_G
Cuts at: 3341 4353 3341
Size: 1012 4269
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2055 794
Size: 1261 4020
BstBI TT'CG_AA
Cuts at: 4085 4085
Size: 5281
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5281
BstXI CCAn_nnnn'nTGG
Cuts at: 2137 2338 2137
Size: 201 5080
BstYI r'GATC_y
Cuts at: 1972 2611 2978 3363 3380 4832 4918 4930 5276
Size: 639 367 385 17 1452 86 12 346
Cuts at: 5276 1972
Size: 1977
Fragments arranged by size:
1977 1452 639 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2043 513
Size: 552 978 3751
Fragments arranged by size:
3751 978 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4542
Fragments arranged by size:
4542 683 56
BtsI GCAGTG_nn'
Cuts at: 2175 2314 2797 3591 3611 2175
Size: 139 483 794 20 3845
Fragments arranged by size:
3845 794 483 139 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5281
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2095 2130 529
Size: 35 1531 35 3680
Fragments arranged by size:
3680 1531 35 35
DraI TTT'AAA
Cuts at: 2585 2585
Size: 5281
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4288 809
Size: 507 2972 1802
Fragments arranged by size:
2972 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2207 2439 2728
Size: 54 1425 50 275 18 377 232 289
Cuts at: 2728 3617 5246 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 377 289 275 232 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2207 2728 1537
Size: 275 395 521 4090
Fragments arranged by size:
4090 521 395 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3211 1353
Size: 1858 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3868 4242 4388 387
Size: 1064 138 2279 374 146 1280
Fragments arranged by size:
2279 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2411 2489 3344 3941 4728 4996 1239
Size: 393 779 78 855 597 787 268 1524
Fragments arranged by size:
1524 855 787 779 597 393 268 78
EcoO109I rG'GnC_Cy
Cuts at: 2428 2428
Size: 5281
EcoRI G'AATT_C
Cuts at: 2590 2968 2590
Size: 378 4903
FspI TGC'GCA
Cuts at: 3787 3787
Size: 5281
HaeII r_GCGC'y
Cuts at: 1678 4428 1678
Size: 2750 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2085 2117 3922 3954 3996
Size: 32 135 32 513 32 1805 32 42
Cuts at: 3996 4028 1373
Size: 32 2626
Fragments arranged by size:
2626 1805 513 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2625 2871 669
Size: 988 281 687 246 3079
Fragments arranged by size:
3079 988 687 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5281
HpaI GTT'AAC
Cuts at: 2871 2871
Size: 5281
KpnI G_GTAC'C
Cuts at: 2606 2606
Size: 5281
MfeI C'AATT_G
Cuts at: 2880 2880
Size: 5281
MluI A'CGCG_T
Cuts at: 5157 5157
Size: 5281
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4395 4579 1305
Size: 3090 184 2007
Fragments arranged by size:
3090 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2441 10
Size: 54 1425 343 609 2850
Fragments arranged by size:
2850 1425 609 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2339 3239 3598 3757 518
Size: 648 1173 900 359 159 2042
Fragments arranged by size:
2042 1173 900 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2697 3374 4522 4767 1606
Size: 1091 677 1148 245 2120
Fragments arranged by size:
2120 1148 1091 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4729
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5281
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5281
NotI GC'GGCC_GC
Cuts at: 2728 2728
Size: 5281
NspI r_CATG'y
Cuts at: 1132 1229 2639 4184 1132
Size: 97 1410 1545 2229
Fragments arranged by size:
2229 1545 1410 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5281
PciI A'CATG_T
Cuts at: 4180 4180
Size: 5281
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5281
PfoI T'CCnGG_A
Cuts at: 2089 2089
Size: 5281
PmeI GTTT'AAAC
Cuts at: 2585 2585
Size: 5281
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3350 3382 4863 4895 3350
Size: 32 1481 32 3736
Fragments arranged by size:
3736 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2231 1613
Size: 618 4663
PsiI TTA'TAA
Cuts at: 2851 2851
Size: 5281
PstI C_TGCA'G
Cuts at: 830 2573 2633 830
Size: 1743 60 3478
Fragments arranged by size:
3478 1743 60
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5281
SalI G'TCGA_C
Cuts at: 1936 2623 1936
Size: 687 4594
SbfI CC_TGCA'GG
Cuts at: 2633 2633
Size: 5281
ScaI AGT'ACT
Cuts at: 3529 5241 3529
Size: 1712 3569
SfcI C'TryA_G
Cuts at: 826 1045 2569 2629 3764 4445 4636 826
Size: 219 1524 60 1135 681 191 1471
Fragments arranged by size:
1524 1471 1135 681 219 191 60
SfiI GGCCn_nnn'nGGCC
Cuts at: 2525 2525
Size: 5281
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5281
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5281
SmaI CCC'GGG
Cuts at: 1872 2608 1872
Size: 736 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3385 4286 4548 820
Size: 197 926 1442 901 262 1553
Fragments arranged by size:
1553 1442 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3072 493
Size: 2579 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5281
SphI G_CATG'C
Cuts at: 1132 2639 1132
Size: 1507 3774
SspI AAT'ATT
Cuts at: 5 52 3205 5
Size: 47 3153 2081
Fragments arranged by size:
3153 2081 47
StuI AGG'CCT
Cuts at: 1517 2496 1517
Size: 979 4302
StyI C'CwwG_G
Cuts at: 513 1065 2442 2720 513
Size: 552 1377 278 3074
Fragments arranged by size:
3074 1377 552 278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2344 2501 3308 3325 3478 3663 4108
Size: 1255 764 157 807 17 153 185 445
Cuts at: 4108 325
Size: 1498
Fragments arranged by size:
1498 1255 807 764 445 185 157 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2097 3527 5239
Size: 274 80 33 51 1443 120 1430 1712
Cuts at: 5239 96
Size: 138
Fragments arranged by size:
1712 1443 1430 274 138 120 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2319 2351 5241 5273 2319
Size: 32 2890 32 2327
Fragments arranged by size:
2890 2327 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5281
XbaI T'CTAG_A
Cuts at: 2617 2617
Size: 5281
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2020 2137 2338 2020
Size: 117 201 4963
Fragments arranged by size:
4963 201 117
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5281
XmaI C'CCGG_G
Cuts at: 1870 2606 1870
Size: 736 4545
XmnI GAAnn'nnTTC
Cuts at: 3410 3410
Size: 5281
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2233 3089 276
Size: 53 83 186 936 699 856 2468
Fragments arranged by size:
2468 936 856 699 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfoI SrfI
SwaI