Restriction map for
pFN21AB8457
(Circular) (Six-base) MAPSORT of: pFN21AB8457.seq Check: 1878 from: 1 to: 5878
pFN21AB8457 5878 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2797 2797
Size: 5878
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3688 278
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3221 1937
Size: 1284 4594
Acc65I G'GTAC_C
Cuts at: 3199 3199
Size: 5878
AclI AA'CG_TT
Cuts at: 1563 4005 4378 1563
Size: 2442 373 3063
Fragments arranged by size:
3063 2442 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3941 5325 5593 1632
Size: 2309 1384 268 1917
Fragments arranged by size:
2309 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5681
AflIII A'CryG_T
Cuts at: 1203 2184 4777 5754 1203
Size: 981 2593 977 1327
Fragments arranged by size:
2593 1327 981 977
AgeI A'CCGG_T
Cuts at: 4709 4709
Size: 5878
AhdI GACnn_n'nnGTC
Cuts at: 4607 4607
Size: 5878
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5878
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5878
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2348 2380 2465 2497 2348
Size: 32 85 32 5729
Fragments arranged by size:
5729 85 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2768 2789 2831 2897 2964 3621 5193 2768
Size: 21 42 66 67 657 1572 3453
Fragments arranged by size:
3453 1572 657 67 66 42 21
ApaI G_GGCC'C
Cuts at: 2616 2891 2616
Size: 275 5603
ApaLI G'TGCA_C
Cuts at: 3935 5091 3935
Size: 1156 4722
ApoI r'AATT_y
Cuts at: 1493 3140 3187 3413 3565 5674 1493
Size: 1647 47 226 152 2109 1697
Fragments arranged by size:
2109 1697 1647 226 152 47
AseI AT'TA_AT
Cuts at: 160 1032 4432 160
Size: 872 3400 1606
Fragments arranged by size:
3400 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
AvaI C'yCGr_G
Cuts at: 1870 1943 2129 2558 2931 3045 3203 1870
Size: 73 186 429 373 114 158 4545
Fragments arranged by size:
4545 429 373 186 158 114 73
BamHI G'GATC_C
Cuts at: 3208 3575 3208
Size: 367 5511
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2030 2284 3082 3199 4654
Size: 325 217 627 243 254 798 117 1455
Cuts at: 4654 618
Size: 1842
Fragments arranged by size:
1842 1455 798 627 325 254 243 217
117
BanII G_rGCy'C
Cuts at: 1391 2559 2616 2891 3197 1391
Size: 1168 57 275 306 4072
Fragments arranged by size:
4072 1168 306 275 57
BbeI G_GCGC'C
Cuts at: 2034 2288 3086 2034
Size: 254 798 4826
Fragments arranged by size:
4826 798 254
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2167 928
Size: 971 268 4639
Fragments arranged by size:
4639 971 268
BbvCI CC'TCA_GC
Cuts at: 2195 2624 2782 2898 3153 2195
Size: 429 158 116 255 4920
Fragments arranged by size:
4920 429 255 158 116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4069 4103 1903
Size: 34 50 34 2048 34 3678
Fragments arranged by size:
3678 2048 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3770 4980 3770
Size: 1210 4668
BclI T'GATC_A
Cuts at: 1548 2115 1548
Size: 567 5311
BfrBI ATG'CAT
Cuts at: 2152 2152
Size: 5878
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4489 136
Size: 107 122 71 631 605 2817 1525
Fragments arranged by size:
2817 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5873 5873
Size: 5878
BlpI GC'TnA_GC
Cuts at: 2417 3295 2417
Size: 878 5000
Bme1580I G_kGCm'C
Cuts at: 1790 2616 2891 3114 3939 5095 1790
Size: 826 275 223 825 1156 2573
Fragments arranged by size:
2573 1156 826 825 275 223
BmgBI CAC'GTC
Cuts at: 1318 2187 1318
Size: 869 5009
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2885 2909 2974
Size: 416 53 83 399 24 1468 24 65
Cuts at: 2974 3101 3609 4567 442
Size: 127 508 958 1753
Fragments arranged by size:
1753 1468 958 508 416 399 127 83
65 53 24 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2443 2661 4538 1239
Size: 1204 218 1877 2579
Fragments arranged by size:
2579 1877 1204 218
Bpu10I CC'TnA_GC
Cuts at: 2195 2624 2782 2898 3153 2195
Size: 429 158 116 255 4920
Fragments arranged by size:
4920 429 255 158 116
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4003 4868 5166 4003
Size: 865 298 4715
Fragments arranged by size:
4715 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2523 4541 882
Size: 617 1024 2018 2219
Fragments arranged by size:
2219 2018 1024 617
BsaAI yAC'GTr
Cuts at: 493 1204 3669 493
Size: 711 2465 2702
Fragments arranged by size:
2702 2465 711
BsaBI GATnn'nnATC
Cuts at: 5872 5872
Size: 5878
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2031 2285 3083 3685
Size: 53 83 186 936 498 254 798 602
Cuts at: 3685 4067 4702 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 798 635 602 498 382 254
186 83 53
BsaWI w'CCGG_w
Cuts at: 4311 4709 4983 5130 5541 4311
Size: 398 274 147 411 4648
Fragments arranged by size:
4648 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2348 2378 2441 2471 1178
Size: 30 165 30 945 30 63 30 4585
Fragments arranged by size:
4585 945 165 63 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2612 3170 1186
Size: 1426 558 3894
Fragments arranged by size:
3894 1426 558
BseYI C'CCAG_C
Cuts at: 2070 2373 2698 2740 2761 2824 2879 2976 3113
Size: 303 325 42 21 63 55 97 137
Cuts at: 3113 5081 5577 2070
Size: 1968 496 2371
Fragments arranged by size:
2371 1968 496 325 303 137 97 63
55 42 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2298 2470 1163
Size: 573 562 172 4571
Fragments arranged by size:
4571 573 562 172
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3328 4089 5117 1540
Size: 275 186 1327 761 1028 2301
Fragments arranged by size:
2301 1327 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2225 3939 4024 5095 2225
Size: 1714 85 1071 3008
Fragments arranged by size:
3008 1714 1071 85
BsmI GAATG_Cn'
Cuts at: 3389 3482 3389
Size: 93 5785
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2225 2559 2616 2891 3114 3197 3939
Size: 399 435 334 57 275 223 83 742
Cuts at: 3939 4024 5095 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 435 399 334 275 223
85 83 57
BspEI T'CCGG_A
Cuts at: 5541 5541
Size: 5878
BspHI T'CATG_A
Cuts at: 2643 3767 5497 2643
Size: 1124 1730 3024
Fragments arranged by size:
3024 1730 1124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2797 2947 3233 844
Size: 1953 150 286 3489
Fragments arranged by size:
3489 1953 286 150
BsrBI CCG'CTC
Cuts at: 1394 1823 2508 2571 3022 3765 1394
Size: 429 685 63 451 743 3507
Fragments arranged by size:
3507 743 685 451 429 63
BsrDI GCAATG_nn'
Cuts at: 65 1248 4373 4547 65
Size: 1183 3125 174 1396
Fragments arranged by size:
3125 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2332 4522 4709 1700
Size: 110 522 2190 187 2869
Fragments arranged by size:
2869 2190 522 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5878
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5878
BssSI C'ACGA_G
Cuts at: 2402 3938 4950 2402
Size: 1536 1012 3330
Fragments arranged by size:
3330 1536 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2269 794
Size: 1475 4403
BstBI TT'CG_AA
Cuts at: 4682 4682
Size: 5878
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5878
BstXI CCAn_nnnn'nTGG
Cuts at: 2047 2155 2917 2047
Size: 108 762 5008
Fragments arranged by size:
5008 762 108
BstYI r'GATC_y
Cuts at: 1972 3208 3575 3960 3977 5429 5515 5527 5873
Size: 1236 367 385 17 1452 86 12 346
Cuts at: 5873 1972
Size: 1977
Fragments arranged by size:
1977 1452 1236 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2161 2203 2445 2718 2802 2851 513
Size: 552 1096 42 242 273 84 49 3540
Fragments arranged by size:
3540 1096 552 273 242 84 49 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2213 487
Size: 683 56 987 4152
Fragments arranged by size:
4152 987 683 56
BtsI GCAGTG_nn'
Cuts at: 3394 4188 4208 3394
Size: 794 20 5064
Fragments arranged by size:
5064 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5878
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2188 2223 529
Size: 35 1624 35 4184
Fragments arranged by size:
4184 1624 35 35
DraI TTT'AAA
Cuts at: 3182 3182
Size: 5878
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4885 809
Size: 507 3569 1802
Fragments arranged by size:
3569 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2250 2334 2452
Size: 54 1425 50 275 18 420 84 118
Cuts at: 2452 2986 3325 4214 5843 8
Size: 534 339 889 1629 43
Fragments arranged by size:
1629 1425 889 534 420 339 275 118
84 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3325 1537
Size: 275 1513 4090
Fragments arranged by size:
4090 1513 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3066 3808 1353
Size: 1713 742 3423
Fragments arranged by size:
3423 1713 742
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2129 2582 4465 4839 4985 387
Size: 1064 138 540 453 1883 374 146 1280
Fragments arranged by size:
1883 1280 1064 540 453 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2443 2661 3941 4538 5325 5593 1239
Size: 393 811 218 1280 597 787 268 1524
Fragments arranged by size:
1524 1280 811 787 597 393 268 218
EcoO109I rG'GnC_Cy
Cuts at: 2613 2723 2807 2927 2936 3102 2613
Size: 110 84 120 9 166 5389
Fragments arranged by size:
5389 166 120 110 84 9
EcoRI G'AATT_C
Cuts at: 3187 3565 3187
Size: 378 5500
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2437 2437
Size: 5878
FspI TGC'GCA
Cuts at: 4384 4384
Size: 5878
HaeII r_GCGC'y
Cuts at: 1678 2034 2288 2768 3086 5025 1678
Size: 356 254 480 318 1939 2531
Fragments arranged by size:
2531 1939 480 356 318 254
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2212 2244 2290 2322 2441
Size: 32 135 32 640 32 46 32 119
Cuts at: 2441 2473 2891 2923 4519 4551 4593 4625 1373
Size: 32 418 32 1596 32 42 32 2626
Fragments arranged by size:
2626 1596 640 418 135 119 46 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3222 3468 669
Size: 988 281 1284 246 3079
Fragments arranged by size:
3079 1284 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5878
HpaI GTT'AAC
Cuts at: 3468 3468
Size: 5878
KasI G'GCGC_C
Cuts at: 2030 2284 3082 2030
Size: 254 798 4826
Fragments arranged by size:
4826 798 254
KpnI G_GTAC'C
Cuts at: 3203 3203
Size: 5878
MfeI C'AATT_G
Cuts at: 3477 3477
Size: 5878
MluI A'CGCG_T
Cuts at: 5754 5754
Size: 5878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4992 5176 1305
Size: 3687 184 2007
Fragments arranged by size:
3687 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2252 10
Size: 54 1425 343 420 3636
Fragments arranged by size:
3636 1425 420 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2153 3836 4195 4354 518
Size: 648 987 1683 359 159 2042
Fragments arranged by size:
2042 1683 987 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2266 2669 2687 2879 3294 3971 5119 5364
Size: 660 403 18 192 415 677 1148 245
Cuts at: 5364 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 660 415 403 245 192
18
NarI GG'CG_CC
Cuts at: 2031 2285 3083 2031
Size: 254 798 4826
Fragments arranged by size:
4826 798 254
NcoI C'CATG_G
Cuts at: 513 1065 2851 513
Size: 552 1786 3540
Fragments arranged by size:
3540 1786 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5878
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5878
NotI GC'GGCC_GC
Cuts at: 3325 3325
Size: 5878
NsiI A_TGCA'T
Cuts at: 2154 2154
Size: 5878
NspI r_CATG'y
Cuts at: 1132 1229 2156 3236 4781 1132
Size: 97 927 1080 1545 2229
Fragments arranged by size:
2229 1545 1080 927 97
PasI CC'CwG_GG
Cuts at: 1348 2017 1348
Size: 669 5209
PciI A'CATG_T
Cuts at: 4777 4777
Size: 5878
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5878
PfoI T'CCnGG_A
Cuts at: 2216 2216
Size: 5878
PmeI GTTT'AAAC
Cuts at: 3182 3182
Size: 5878
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2348 2380 3947 3979 5460 5492 2348
Size: 32 1567 32 1481 32 2734
Fragments arranged by size:
2734 1567 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2723 2807 2927 2936 3102 2723
Size: 84 120 9 166 5499
Fragments arranged by size:
5499 166 120 84 9
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5878
PsiI TTA'TAA
Cuts at: 3448 3448
Size: 5878
PspOMI G'GGCC_C
Cuts at: 2612 2887 2612
Size: 275 5603
PstI C_TGCA'G
Cuts at: 830 2110 2265 2668 3230 830
Size: 1280 155 403 562 3478
Fragments arranged by size:
3478 1280 562 403 155
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5878
PvuII CAG'CTG
Cuts at: 2266 2669 2879 2266
Size: 403 210 5265
Fragments arranged by size:
5265 403 210
SalI G'TCGA_C
Cuts at: 1936 3220 1936
Size: 1284 4594
SanDI GG'GwC_CC
Cuts at: 2927 2927
Size: 5878
SbfI CC_TGCA'GG
Cuts at: 3230 3230
Size: 5878
ScaI AGT'ACT
Cuts at: 4126 5838 4126
Size: 1712 4166
SfcI C'TryA_G
Cuts at: 826 1045 2106 2261 2664 3226 4361 5042 5233
Size: 219 1061 155 403 562 1135 681 191
Cuts at: 5233 826
Size: 1471
Fragments arranged by size:
1471 1135 1061 681 562 403 219 191
155
SfoI GGC'GCC
Cuts at: 2032 2286 3084 2032
Size: 254 798 4826
Fragments arranged by size:
4826 798 254
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5878
SmaI CCC'GGG
Cuts at: 1872 2560 3047 3205 1872
Size: 688 487 158 4545
Fragments arranged by size:
4545 688 487 158
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3982 4883 5145 820
Size: 197 926 2039 901 262 1553
Fragments arranged by size:
2039 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3669 493
Size: 3176 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5878
SphI G_CATG'C
Cuts at: 1132 3236 1132
Size: 2104 3774
SrfI GCCC'GGGC
Cuts at: 2560 3047 2560
Size: 487 5391
SspI AAT'ATT
Cuts at: 5 52 3802 5
Size: 47 3750 2081
Fragments arranged by size:
3750 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5878
StyI C'CwwG_G
Cuts at: 513 1065 2608 2851 3317 513
Size: 552 1543 243 466 3074
Fragments arranged by size:
3074 1543 552 466 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2062 2139 2240 2541 3905 3922 4075
Size: 1255 482 77 101 301 1364 17 153
Cuts at: 4075 4260 4705 325
Size: 185 445 1498
Fragments arranged by size:
1498 1364 1255 482 445 301 185 153
101 77 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4124 5836 96
Size: 274 80 33 51 1443 2147 1712 138
Fragments arranged by size:
2147 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3059 3091 5838 5870 3059
Size: 32 2747 32 3067
Fragments arranged by size:
3067 2747 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2859 1316
Size: 1543 4335
XbaI T'CTAG_A
Cuts at: 3214 3214
Size: 5878
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2044 2467 2044
Size: 423 5455
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5878
XmaI C'CCGG_G
Cuts at: 1870 2558 3045 3203 1870
Size: 688 487 158 4545
Fragments arranged by size:
4545 688 487 158
XmnI GAAnn'nnTTC
Cuts at: 4007 4007
Size: 5878
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3686 276
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BplI BsiWI BsmBI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI
NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI
SacII SapI SexAI SfiI SwaI