Restriction map for
pFN21AB8454
(Circular) (Six-base) MAPSORT of: pFN21AB8454.seq Check: 6949 from: 1 to: 6304
pFN21AB8454 6304 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2940 4114 278
Size: 53 83 186 936 1404 1174 2468
Fragments arranged by size:
2468 1404 1174 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3647 1937
Size: 1710 4594
Acc65I G'GTAC_C
Cuts at: 2454 3625 2454
Size: 1171 5133
AclI AA'CG_TT
Cuts at: 1563 4431 4804 1563
Size: 2868 373 3063
Fragments arranged by size:
3063 2868 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3163 3488 4367 5751 6019 1632
Size: 1531 325 879 1384 268 1917
Fragments arranged by size:
1917 1531 1384 879 325 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6107
AflIII A'CryG_T
Cuts at: 1203 3444 5203 6180 1203
Size: 2241 1759 977 1327
Fragments arranged by size:
2241 1759 1327 977
AgeI A'CCGG_T
Cuts at: 3480 5135 3480
Size: 1655 4649
AhdI GACnn_n'nnGTC
Cuts at: 5033 5033
Size: 6304
AleI CACnn'nnGTG
Cuts at: 1166 2564 1166
Size: 1398 4906
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2390 3161 3254 3465 1184
Size: 1206 771 93 211 4023
Fragments arranged by size:
4023 1206 771 211 93
AlwNI CAG_nnn'CTG
Cuts at: 2254 4047 5619 2254
Size: 1793 1572 2939
Fragments arranged by size:
2939 1793 1572
ApaLI G'TGCA_C
Cuts at: 4361 5517 4361
Size: 1156 5148
ApoI r'AATT_y
Cuts at: 1493 3613 3839 3991 6100 1493
Size: 2120 226 152 2109 1697
Fragments arranged by size:
2120 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4858 160
Size: 872 3826 1606
Fragments arranged by size:
3826 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6304
AvaI C'yCGr_G
Cuts at: 1870 1943 2109 2189 2306 2392 2469 3278 3557
Size: 73 166 80 117 86 77 809 279
Cuts at: 3557 3576 3629 1870
Size: 19 53 4545
Fragments arranged by size:
4545 809 279 166 117 86 80 77
73 53 19
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2438 2471 2438
Size: 33 6271
BamHI G'GATC_C
Cuts at: 3634 4001 3634
Size: 367 5937
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2366 2454 2492 2602 2614
Size: 325 217 627 579 88 38 110 12
Cuts at: 2614 2783 3074 3543 3625 5080 618
Size: 169 291 469 82 1455 1842
Fragments arranged by size:
1842 1455 627 579 469 325 291 217
169 110 88 82 38 12
BanII G_rGCy'C
Cuts at: 1391 2283 3021 3519 3623 1391
Size: 892 738 498 104 4072
Fragments arranged by size:
4072 892 738 498 104
BbeI G_GCGC'C
Cuts at: 2496 3547 2496
Size: 1051 5253
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2812 2932 928
Size: 971 913 120 4300
Fragments arranged by size:
4300 971 913 120
BbvCI CC'TCA_GC
Cuts at: 2126 2126
Size: 6304
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4495 4529 1903
Size: 34 2558 34 3678
Fragments arranged by size:
3678 2558 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4196 5406 4196
Size: 1210 5094
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6304
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2529 2533 4915
Size: 107 122 71 631 605 857 4 2382
Cuts at: 4915 136
Size: 1525
Fragments arranged by size:
2382 1525 857 631 605 122 107 71
4
BglII A'GATC_T
Cuts at: 6299 6299
Size: 6304
BlpI GC'TnA_GC
Cuts at: 3721 3721
Size: 6304
Bme1580I G_kGCm'C
Cuts at: 1790 2559 2607 3077 4365 5521 1790
Size: 769 48 470 1288 1156 2573
Fragments arranged by size:
2573 1288 1156 769 470 48
BmgBI CAC'GTC
Cuts at: 1318 2671 2695 1318
Size: 1353 24 4927
Fragments arranged by size:
4927 1353 24
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4035 4993 442
Size: 416 53 83 399 24 2618 958 1753
Fragments arranged by size:
2618 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6304
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2399 3101 4964 1239
Size: 1160 702 1863 2579
Fragments arranged by size:
2579 1863 1160 702
Bpu10I CC'TnA_GC
Cuts at: 2126 2462 2126
Size: 336 5968
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2741 2978 4429 5294 5592 2741
Size: 237 1451 865 298 3453
Fragments arranged by size:
3453 1451 865 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4967 882
Size: 617 3468 2219
Fragments arranged by size:
3468 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4095 493
Size: 711 2891 2702
Fragments arranged by size:
2891 2702 711
BsaBI GATnn'nnATC
Cuts at: 6298 6298
Size: 6304
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2349 2493 2703 2904
Size: 53 83 186 936 816 144 210 201
Cuts at: 2904 2937 2982 3544 4111 4493 5128 275
Size: 33 45 562 567 382 635 1451
Fragments arranged by size:
1451 936 816 635 567 562 382 210
201 186 144 83 53 45 33
BsaWI w'CCGG_w
Cuts at: 3480 4737 5135 5409 5556 5967 3480
Size: 1257 398 274 147 411 3817
Fragments arranged by size:
3817 1257 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2504 2534 3074 3104 1178
Size: 30 165 30 1101 30 540 30 4378
Fragments arranged by size:
4378 1101 540 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2291 2420 2422 1186
Size: 1105 129 2 5068
Fragments arranged by size:
5068 1105 129 2
BseYI C'CCAG_C
Cuts at: 2261 2543 2686 3226 3330 3434 5507 6003 2261
Size: 282 143 540 104 104 2073 496 2562
Fragments arranged by size:
2562 2073 540 496 282 143 104 104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2057 2588 3082 3224 3236 1163
Size: 573 321 531 494 142 12 4231
Fragments arranged by size:
4231 573 531 494 321 142 12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2274 2402 2753 2848 3481 3754
Size: 275 186 273 128 351 95 633 273
Cuts at: 3754 4515 5543 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 633 351 275 273 273
186 128 95
BsiHKAI G_wGCw'C
Cuts at: 2283 2814 3444 3527 4365 4450 5521 2283
Size: 531 630 83 838 85 1071 3066
Fragments arranged by size:
3066 1071 838 630 531 85 83
BsmI GAATG_Cn'
Cuts at: 3815 3908 3815
Size: 93 6211
BsmBI CGTCTCn'nnnn_
Cuts at: 2008 2911 2008
Size: 903 5401
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2283 2559 2607 2814 3021 3077 3444
Size: 399 493 276 48 207 207 56 367
Cuts at: 3444 3519 3527 3623 4365 4450 5521 1391
Size: 75 8 96 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 493 399 367 276 207
207 96 85 75 56 48 8
BspEI T'CCGG_A
Cuts at: 5967 5967
Size: 6304
BspHI T'CATG_A
Cuts at: 4193 5923 4193
Size: 1730 4574
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2217 2589 2670 2886 2931 3471 3501 3659
Size: 1373 372 81 216 45 540 30 158
Cuts at: 3659 844
Size: 3489
Fragments arranged by size:
3489 1373 540 372 216 158 81 45
30
BsrBI CCG'CTC
Cuts at: 1394 1823 2596 2755 4191 1394
Size: 429 773 159 1436 3507
Fragments arranged by size:
3507 1436 773 429 159
BsrDI GCAATG_nn'
Cuts at: 65 1248 4799 4973 65
Size: 1183 3551 174 1396
Fragments arranged by size:
3551 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2048 2342 2847 3200 3248 3480 4948
Size: 110 238 294 505 353 48 232 1468
Cuts at: 4948 5135 1700
Size: 187 2869
Fragments arranged by size:
2869 1468 505 353 294 238 232 187
110 48
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6304
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6304
BssSI C'ACGA_G
Cuts at: 2651 2681 3299 4364 5376 2651
Size: 30 618 1065 1012 3579
Fragments arranged by size:
3579 1065 1012 618 30
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2254 794
Size: 1460 4844
BstBI TT'CG_AA
Cuts at: 5108 5108
Size: 6304
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6304
BstXI CCAn_nnnn'nTGG
Cuts at: 2377 2377
Size: 6304
BstYI r'GATC_y
Cuts at: 1972 2964 3634 4001 4386 4403 5855 5941 5953
Size: 992 670 367 385 17 1452 86 12
Cuts at: 5953 6299 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 992 670 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2415 2415
Size: 6304
BtgI C'CryG_G
Cuts at: 513 1065 2747 3370 513
Size: 552 1682 623 3447
Fragments arranged by size:
3447 1682 623 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5565
Fragments arranged by size:
5565 683 56
BtsI GCAGTG_nn'
Cuts at: 3126 3224 3256 3820 4614 4634 3126
Size: 98 32 564 794 20 4796
Fragments arranged by size:
4796 794 564 98 32 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6304
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6269
DraI TTT'AAA
Cuts at: 3608 3608
Size: 6304
DraIII CAC_nnn'GTG
Cuts at: 2290 2809 2290
Size: 519 5785
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5311 809
Size: 507 3995 1802
Fragments arranged by size:
3995 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2271 2399
Size: 54 1425 50 275 18 216 225 128
Cuts at: 2399 2444 2750 2845 3202 3751 4640 6269 8
Size: 45 306 95 357 549 889 1629 43
Fragments arranged by size:
1629 1425 889 549 357 306 275 225
216 128 95 54 50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2271 2399 2750 2845 3751 1537
Size: 275 459 128 351 95 906 4090
Fragments arranged by size:
4090 906 459 351 275 128 95
EarI CTCTTCn'nnn_
Cuts at: 1353 4234 1353
Size: 2881 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2789 3131 3295 3611 4891 5265
Size: 1064 138 1200 342 164 316 1280 374
Cuts at: 5265 5411 387
Size: 146 1280
Fragments arranged by size:
1280 1280 1200 1064 374 342 316 164
146 138
EcoICRI GAG'CTC
Cuts at: 2281 2281
Size: 6304
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2399 3101 3163 3488 4367 4964 5751
Size: 393 767 702 62 325 879 597 787
Cuts at: 5751 6019 1239
Size: 268 1524
Fragments arranged by size:
1524 879 787 767 702 597 393 325
268 62
EcoNI CCTnn'n_nnAGG
Cuts at: 2894 2894
Size: 6304
EcoO109I rG'GnC_Cy
Cuts at: 2201 3355 3554 2201
Size: 1154 199 4951
Fragments arranged by size:
4951 1154 199
EcoRI G'AATT_C
Cuts at: 3613 3991 3613
Size: 378 5926
FseI GG_CCGG'CC
Cuts at: 2052 2052
Size: 6304
FspI TGC'GCA
Cuts at: 2333 2882 2918 3038 4810 2333
Size: 549 36 120 1772 3827
Fragments arranged by size:
3827 1772 549 120 36
FspAI rTGC'GCAy
Cuts at: 2333 2333
Size: 6304
HaeII r_GCGC'y
Cuts at: 1678 2469 2496 3547 5451 1678
Size: 791 27 1051 1904 2531
Fragments arranged by size:
2531 1904 1051 791 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2891 2923 3074 3106 4945
Size: 32 135 32 1319 32 151 32 1839
Cuts at: 4945 4977 5019 5051 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1839 1319 151 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2647 3648 3894 669
Size: 988 281 709 1001 246 3079
Fragments arranged by size:
3079 1001 988 709 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6304
HpaI GTT'AAC
Cuts at: 3894 3894
Size: 6304
KasI G'GCGC_C
Cuts at: 2492 3543 2492
Size: 1051 5253
KpnI G_GTAC'C
Cuts at: 2458 3629 2458
Size: 1171 5133
MfeI C'AATT_G
Cuts at: 3903 3903
Size: 6304
MluI A'CGCG_T
Cuts at: 6180 6180
Size: 6304
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2601 2748 5418 5602 1305
Size: 1296 147 2670 184 2007
Fragments arranged by size:
2670 2007 1296 184 147
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4482
Fragments arranged by size:
4482 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2372 2564 3212 3447 4262 4621
Size: 648 843 363 192 648 235 815 359
Cuts at: 4621 4780 518
Size: 159 2042
Fragments arranged by size:
2042 843 815 648 648 363 359 235
192 159
MspA1I CmG'CkG
Cuts at: 1606 2254 2547 2749 3372 3720 4397 5545 5790
Size: 648 293 202 623 348 677 1148 245
Cuts at: 5790 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 648 623 348 293 245
202
NaeI GCC'GGC
Cuts at: 2050 2344 2849 3250 2050
Size: 294 505 401 5104
Fragments arranged by size:
5104 505 401 294
NarI GG'CG_CC
Cuts at: 2493 3544 2493
Size: 1051 5253
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5752
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6304
NgoMIV G'CCGG_C
Cuts at: 2048 2342 2847 3248 2048
Size: 294 505 401 5104
Fragments arranged by size:
5104 505 401 294
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6304
NotI GC'GGCC_GC
Cuts at: 2750 3751 2750
Size: 1001 5303
NspI r_CATG'y
Cuts at: 1132 1229 3037 3112 3448 3662 5207 1132
Size: 97 1808 75 336 214 1545 2229
Fragments arranged by size:
2229 1808 1545 336 214 97 75
PasI CC'CwG_GG
Cuts at: 1348 2607 1348
Size: 1259 5045
PciI A'CATG_T
Cuts at: 3444 5203 3444
Size: 1759 4545
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2290 2686 3211 1108
Size: 1182 396 525 4201
Fragments arranged by size:
4201 1182 525 396
PfoI T'CCnGG_A
Cuts at: 2763 2823 2763
Size: 60 6244
PmeI GTTT'AAAC
Cuts at: 3608 3608
Size: 6304
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4373 4405 5886 5918 4373
Size: 32 1481 32 4759
Fragments arranged by size:
4759 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3355 3554 3355
Size: 199 6105
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6304
PsiI TTA'TAA
Cuts at: 3874 3874
Size: 6304
PstI C_TGCA'G
Cuts at: 830 2250 3117 3430 3656 830
Size: 1420 867 313 226 3478
Fragments arranged by size:
3478 1420 867 313 226
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6304
PvuII CAG'CTG
Cuts at: 2547 2547
Size: 6304
SacI G_AGCT'C
Cuts at: 2283 2283
Size: 6304
SacII CC_GC'GG
Cuts at: 2750 3373 2750
Size: 623 5681
SalI G'TCGA_C
Cuts at: 1936 3646 1936
Size: 1710 4594
SanDI GG'GwC_CC
Cuts at: 3554 3554
Size: 6304
SbfI CC_TGCA'GG
Cuts at: 3656 3656
Size: 6304
ScaI AGT'ACT
Cuts at: 2573 3161 4552 6264 2573
Size: 588 1391 1712 2613
Fragments arranged by size:
2613 1712 1391 588
SexAI A'CCwGG_T
Cuts at: 2136 2136
Size: 6304
SfcI C'TryA_G
Cuts at: 826 1045 2246 3113 3426 3652 4787 5468 5659
Size: 219 1201 867 313 226 1135 681 191
Cuts at: 5659 826
Size: 1471
Fragments arranged by size:
1471 1201 1135 867 681 313 226 219
191
SfoI GGC'GCC
Cuts at: 2494 3545 2494
Size: 1051 5253
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6304
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6304
SmaI CCC'GGG
Cuts at: 1872 2394 2471 3578 3631 1872
Size: 522 77 1107 53 4545
Fragments arranged by size:
4545 1107 522 77 53
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2756 2993 4408 5309 5571 820
Size: 197 926 813 237 1415 901 262 1553
Fragments arranged by size:
1553 1415 926 901 813 262 237 197
SnaBI TAC'GTA
Cuts at: 493 4095 493
Size: 3602 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6304
SphI G_CATG'C
Cuts at: 1132 3662 1132
Size: 2530 3774
SspI AAT'ATT
Cuts at: 5 52 4228 5
Size: 47 4176 2081
Fragments arranged by size:
4176 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6304
StyI C'CwwG_G
Cuts at: 513 1065 2337 2589 3743 513
Size: 552 1272 252 1154 3074
Fragments arranged by size:
3074 1272 1154 552 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2497 2909 3029 4331 4348 4501 4686
Size: 1255 917 412 120 1302 17 153 185
Cuts at: 4686 5131 325
Size: 445 1498
Fragments arranged by size:
1498 1302 1255 917 445 412 185 153
120 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2571 2793 3159
Size: 274 80 33 51 1443 594 222 366
Cuts at: 3159 4550 6262 96
Size: 1391 1712 138
Fragments arranged by size:
1712 1443 1391 594 366 274 222 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2504 2536 6264 6296 2504
Size: 32 3728 32 2512
Fragments arranged by size:
3728 2512 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2089 2903 2936 2981 1316
Size: 773 814 33 45 4639
Fragments arranged by size:
4639 814 773 45 33
XbaI T'CTAG_A
Cuts at: 3640 3640
Size: 6304
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2596 2596
Size: 6304
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6304
XmaI C'CCGG_G
Cuts at: 1870 2392 2469 3576 3629 1870
Size: 522 77 1107 53 4545
Fragments arranged by size:
4545 1107 522 77 53
XmnI GAAnn'nnTTC
Cuts at: 4433 4433
Size: 6304
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2938 4112 276
Size: 53 83 186 936 1404 1174 2468
Fragments arranged by size:
2468 1404 1174 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BfrBI BplI
BsiWI BstZ17I EcoRV FalI NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SapI SfiI SrfI SwaI