Restriction map for
pFN21AB8452
(Circular) (Six-base) MAPSORT of: pFN21AB8452.seq Check: 9499 from: 1 to: 5776
pFN21AB8452 5776 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3586 278
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3119 1937
Size: 1182 4594
Acc65I G'GTAC_C
Cuts at: 2544 3097 2544
Size: 553 5223
AclI AA'CG_TT
Cuts at: 1563 3903 4276 1563
Size: 2340 373 3063
Fragments arranged by size:
3063 2340 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2438 2456 2495 2699 3839 5223 5491 1632
Size: 806 18 39 204 1140 1384 268 1917
Fragments arranged by size:
1917 1384 1140 806 268 204 39 18
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5579
AflIII A'CryG_T
Cuts at: 1203 4675 5652 1203
Size: 3472 977 1327
Fragments arranged by size:
3472 1327 977
AgeI A'CCGG_T
Cuts at: 4607 4607
Size: 5776
AhdI GACnn_n'nnGTC
Cuts at: 2602 4505 2602
Size: 1903 3873
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5776
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5776
AlwNI CAG_nnn'CTG
Cuts at: 2416 2731 3519 5091 2416
Size: 315 788 1572 3101
Fragments arranged by size:
3101 1572 788 315
ApaLI G'TGCA_C
Cuts at: 3833 4989 3833
Size: 1156 4620
ApoI r'AATT_y
Cuts at: 1493 3085 3311 3463 5572 1493
Size: 1592 226 152 2109 1697
Fragments arranged by size:
2109 1697 1592 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4330 160
Size: 872 3298 1606
Fragments arranged by size:
3298 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
AvaI C'yCGr_G
Cuts at: 1870 1943 2107 2555 3101 1870
Size: 73 164 448 546 4545
Fragments arranged by size:
4545 546 448 164 73
BamHI G'GATC_C
Cuts at: 2391 3106 3473 2391
Size: 715 367 4694
Fragments arranged by size:
4694 715 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2111 2342 2544 3097 4552
Size: 325 217 627 324 231 202 553 1455
Cuts at: 4552 618
Size: 1842
Fragments arranged by size:
1842 1455 627 553 325 324 231 217
202
BanII G_rGCy'C
Cuts at: 1391 2012 2108 2554 2937 3095 1391
Size: 621 96 446 383 158 4072
Fragments arranged by size:
4072 621 446 383 158 96
BbeI G_GCGC'C
Cuts at: 2115 2115
Size: 5776
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2469 928
Size: 971 570 4235
Fragments arranged by size:
4235 971 570
BbvCI CC'TCA_GC
Cuts at: 2426 2426
Size: 5776
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3967 4001 1903
Size: 34 2030 34 3678
Fragments arranged by size:
3678 2030 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3668 4878 3668
Size: 1210 4566
BclI T'GATC_A
Cuts at: 1548 2631 1548
Size: 1083 4693
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2151 2504 3037
Size: 107 122 71 631 605 479 353 533
Cuts at: 3037 4387 136
Size: 1350 1525
Fragments arranged by size:
1525 1350 631 605 533 479 353 122
107 71
BglII A'GATC_T
Cuts at: 5771 5771
Size: 5776
BlpI GC'TnA_GC
Cuts at: 3020 3193 3020
Size: 173 5603
Bme1580I G_kGCm'C
Cuts at: 1790 2345 3837 4993 1790
Size: 555 1492 1156 2573
Fragments arranged by size:
2573 1492 1156 555
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5776
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2532 3507 4465
Size: 416 53 83 399 24 1115 975 958
Cuts at: 4465 442
Size: 1753
Fragments arranged by size:
1753 1115 975 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2858 4436 1239
Size: 1619 1578 2579
Fragments arranged by size:
2579 1619 1578
Bpu10I CC'TnA_GC
Cuts at: 2157 2426 2157
Size: 269 5507
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2222 3901 4766 5064 2222
Size: 1679 865 298 2934
Fragments arranged by size:
2934 1679 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2288 3061 4439 882
Size: 617 789 773 1378 2219
Fragments arranged by size:
2219 1378 789 773 617
BsaAI yAC'GTr
Cuts at: 493 1204 2530 3567 493
Size: 711 1326 1037 2702
Fragments arranged by size:
2702 1326 1037 711
BsaBI GATnn'nnATC
Cuts at: 5770 5770
Size: 5776
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2112 2705 3068 3583
Size: 53 83 186 936 579 593 363 515
Cuts at: 3583 3965 4600 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 635 593 579 515 382 363
186 83 53
BsaWI w'CCGG_w
Cuts at: 2388 4209 4607 4881 5028 5439 2388
Size: 1821 398 274 147 411 2725
Fragments arranged by size:
2725 1821 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5551
Fragments arranged by size:
5551 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5776
BseYI C'CCAG_C
Cuts at: 3043 4979 5475 3043
Size: 1936 496 3344
Fragments arranged by size:
3344 1936 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2744 2900 1163
Size: 573 1008 156 4039
Fragments arranged by size:
4039 1008 573 156
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2136 2146 3226 3987 5015 1540
Size: 275 186 135 10 1080 761 1028 2301
Fragments arranged by size:
2301 1080 1028 761 275 186 135 10
BsiHKAI G_wGCw'C
Cuts at: 2012 2132 3021 3837 3922 4993 2012
Size: 120 889 816 85 1071 2795
Fragments arranged by size:
2795 1071 889 816 120 85
BsmI GAATG_Cn'
Cuts at: 3287 3380 3287
Size: 93 5683
BsmBI CGTCTCn'nnnn_
Cuts at: 3059 3059
Size: 5776
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2108 2132 2345 2554 2937 3021
Size: 399 222 96 24 213 209 383 84
Cuts at: 3021 3095 3837 3922 4993 1391
Size: 74 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 383 222 213 209
96 85 84 74 24
BspEI T'CCGG_A
Cuts at: 5439 5439
Size: 5776
BspHI T'CATG_A
Cuts at: 2361 2688 3665 5395 2361
Size: 327 977 1730 2742
Fragments arranged by size:
2742 1730 977 327
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2100 3131 844
Size: 1256 1031 3489
Fragments arranged by size:
3489 1256 1031
BsrBI CCG'CTC
Cuts at: 1394 1823 3663 1394
Size: 429 1840 3507
Fragments arranged by size:
3507 1840 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4271 4445 65
Size: 1183 3023 174 1396
Fragments arranged by size:
3023 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2150 4420 4607 1700
Size: 110 340 2270 187 2869
Fragments arranged by size:
2869 2270 340 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5776
BssHII G'CGCG_C
Cuts at: 1925 2074 2212 1925
Size: 149 138 5489
Fragments arranged by size:
5489 149 138
BssSI C'ACGA_G
Cuts at: 2252 2402 3836 4848 2252
Size: 150 1434 1012 3180
Fragments arranged by size:
3180 1434 1012 150
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2731 794
Size: 1937 3839
BstBI TT'CG_AA
Cuts at: 4580 4580
Size: 5776
BstEII G'GTnAC_C
Cuts at: 1184 2050 2533 1184
Size: 866 483 4427
Fragments arranged by size:
4427 866 483
BstYI r'GATC_y
Cuts at: 1972 2391 2478 3106 3473 3858 3875 5327 5413
Size: 419 87 628 367 385 17 1452 86
Cuts at: 5413 5425 5771 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 628 419 385 367 346 87
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2100 2136 2607 513
Size: 552 1035 36 471 3682
Fragments arranged by size:
3682 1035 552 471 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2754 487
Size: 683 56 1528 3509
Fragments arranged by size:
3509 1528 683 56
BtsI GCAGTG_nn'
Cuts at: 2035 3292 4086 4106 2035
Size: 1257 794 20 3705
Fragments arranged by size:
3705 1257 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5741
DraI TTT'AAA
Cuts at: 3080 3080
Size: 5776
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4783 809
Size: 507 3467 1802
Fragments arranged by size:
3467 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 2143 2163
Size: 54 1425 50 275 18 303 10 20
Cuts at: 2163 2496 3223 4112 5741 8
Size: 333 727 889 1629 43
Fragments arranged by size:
1629 1425 889 727 333 303 275 54
50 43 20 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 2133 2143 3223 1537
Size: 275 321 10 1080 4090
Fragments arranged by size:
4090 1080 321 275 10
EarI CTCTTCn'nnn_
Cuts at: 1353 3706 1353
Size: 2353 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2004 2639 4363 4737 4883 387
Size: 1064 138 415 635 1724 374 146 1280
Fragments arranged by size:
1724 1280 1064 635 415 374 146 138
EcoICRI GAG'CTC
Cuts at: 2010 2010
Size: 5776
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2438 2456 2495 2699 2858 3839 4436
Size: 393 806 18 39 204 159 981 597
Cuts at: 4436 5223 5491 1239
Size: 787 268 1524
Fragments arranged by size:
1524 981 806 787 597 393 268 204
159 39 18
EcoO109I rG'GnC_Cy
Cuts at: 2089 2431 2764 2089
Size: 342 333 5101
Fragments arranged by size:
5101 342 333
EcoRI G'AATT_C
Cuts at: 3085 3463 3085
Size: 378 5398
FspI TGC'GCA
Cuts at: 4282 4282
Size: 5776
HaeII r_GCGC'y
Cuts at: 1678 2115 2157 2891 4923 1678
Size: 437 42 734 2032 2531
Fragments arranged by size:
2531 2032 734 437 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2588 2620 2780 2812 4417
Size: 32 135 32 1016 32 160 32 1605
Cuts at: 4417 4449 4491 4523 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1605 1016 160 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2974 3120 3366 669
Size: 988 281 1036 146 246 3079
Fragments arranged by size:
3079 1036 988 281 246 146
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5776
HpaI GTT'AAC
Cuts at: 3366 3366
Size: 5776
KasI G'GCGC_C
Cuts at: 2111 2111
Size: 5776
KpnI G_GTAC'C
Cuts at: 2548 3101 2548
Size: 553 5223
MfeI C'AATT_G
Cuts at: 3375 3375
Size: 5776
MluI A'CGCG_T
Cuts at: 5652 5652
Size: 5776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4890 5074 1305
Size: 3585 184 2007
Fragments arranged by size:
3585 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2165 2498 10
Size: 54 1425 343 333 333 3288
Fragments arranged by size:
3288 1425 343 333 333 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2693 3734 4093 4252 518
Size: 648 1527 1041 359 159 2042
Fragments arranged by size:
2042 1527 1041 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2677 2728 2869 3043 3192 3869 5017 5262
Size: 1071 51 141 174 149 677 1148 245
Cuts at: 5262 1606
Size: 2120
Fragments arranged by size:
2120 1148 1071 677 245 174 149 141
51
NarI GG'CG_CC
Cuts at: 2112 2112
Size: 5776
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5224
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5776
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5776
NotI GC'GGCC_GC
Cuts at: 3223 3223
Size: 5776
NspI r_CATG'y
Cuts at: 1132 1229 2248 3134 4679 1132
Size: 97 1019 886 1545 2229
Fragments arranged by size:
2229 1545 1019 886 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5776
PciI A'CATG_T
Cuts at: 4675 4675
Size: 5776
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5776
PfoI T'CCnGG_A
Cuts at: 2481 2835 2481
Size: 354 5422
PmeI GTTT'AAAC
Cuts at: 3080 3080
Size: 5776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3845 3877 5358 5390 3845
Size: 32 1481 32 4231
Fragments arranged by size:
4231 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2089 2431 2089
Size: 342 5434
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5776
PsiI TTA'TAA
Cuts at: 3346 3346
Size: 5776
PstI C_TGCA'G
Cuts at: 830 2097 2736 3128 830
Size: 1267 639 392 3478
Fragments arranged by size:
3478 1267 639 392
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5776
PvuII CAG'CTG
Cuts at: 2677 3043 2677
Size: 366 5410
SacI G_AGCT'C
Cuts at: 2012 2012
Size: 5776
SalI G'TCGA_C
Cuts at: 1936 3118 1936
Size: 1182 4594
SbfI CC_TGCA'GG
Cuts at: 3128 3128
Size: 5776
ScaI AGT'ACT
Cuts at: 4024 5736 4024
Size: 1712 4064
SexAI A'CCwGG_T
Cuts at: 2322 2322
Size: 5776
SfcI C'TryA_G
Cuts at: 826 1045 2093 2732 3124 4259 4940 5131 826
Size: 219 1048 639 392 1135 681 191 1471
Fragments arranged by size:
1471 1135 1048 681 639 392 219 191
SfoI GGC'GCC
Cuts at: 2113 2113
Size: 5776
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
SgrAI Cr'CCGG_yG
Cuts at: 1700 2150 1700
Size: 450 5326
SmaI CCC'GGG
Cuts at: 1872 3103 1872
Size: 1231 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2237 3880 4781 5043 820
Size: 197 926 294 1643 901 262 1553
Fragments arranged by size:
1643 1553 926 901 294 262 197
SnaBI TAC'GTA
Cuts at: 493 3567 493
Size: 3074 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5776
SphI G_CATG'C
Cuts at: 1132 3134 1132
Size: 2002 3774
SspI AAT'ATT
Cuts at: 5 52 3700 5
Size: 47 3648 2081
Fragments arranged by size:
3648 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5776
StyI C'CwwG_G
Cuts at: 513 1065 3060 3215 513
Size: 552 1995 155 3074
Fragments arranged by size:
3074 1995 552 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3803 3820 3973 4158 4603 325
Size: 1255 2223 17 153 185 445 1498
Fragments arranged by size:
2223 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4022 5734 96
Size: 274 80 33 51 1443 2045 1712 138
Fragments arranged by size:
2045 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5736 5768 5736
Size: 32 5744
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5776
XbaI T'CTAG_A
Cuts at: 3112 3112
Size: 5776
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5776
XmaI C'CCGG_G
Cuts at: 1870 3101 1870
Size: 1231 4545
XmnI GAAnn'nnTTC
Cuts at: 3905 3905
Size: 5776
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3584 276
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SfiI SrfI SwaI XcmI