Restriction map for
pFN21AB8451
(Circular) (Six-base) MAPSORT of: pFN21AB8451.seq Check: 669 from: 1 to: 5797
pFN21AB8451 5797 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2271 3607 278
Size: 53 83 186 936 735 1336 2468
Fragments arranged by size:
2468 1336 936 735 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3140 1937
Size: 1203 4594
Acc65I G'GTAC_C
Cuts at: 2565 3118 2565
Size: 553 5244
AclI AA'CG_TT
Cuts at: 1563 3924 4297 1563
Size: 2361 373 3063
Fragments arranged by size:
3063 2361 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2258 2284 2438 2477 2720 2911 3860 5244
Size: 626 26 154 39 243 191 949 1384
Cuts at: 5244 5512 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 949 626 268 243 191 154
39 26
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5600
AflIII A'CryG_T
Cuts at: 1203 4696 5673 1203
Size: 3493 977 1327
Fragments arranged by size:
3493 1327 977
AgeI A'CCGG_T
Cuts at: 4628 4628
Size: 5797
AhdI GACnn_n'nnGTC
Cuts at: 4526 4526
Size: 5797
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5797
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5797
AlwNI CAG_nnn'CTG
Cuts at: 2041 2074 2083 2188 2368 2407 2788 3540 5112
Size: 33 9 105 180 39 381 752 1572
Cuts at: 5112 2041
Size: 2726
Fragments arranged by size:
2726 1572 752 381 180 105 39 33
9
ApaI G_GGCC'C
Cuts at: 2015 2015
Size: 5797
ApaLI G'TGCA_C
Cuts at: 3854 5010 3854
Size: 1156 4641
ApoI r'AATT_y
Cuts at: 1493 3106 3332 3484 5593 1493
Size: 1613 226 152 2109 1697
Fragments arranged by size:
2109 1697 1613 226 152
AscI GG'CGCG_CC
Cuts at: 2519 2519
Size: 5797
AseI AT'TA_AT
Cuts at: 160 1032 4351 160
Size: 872 3319 1606
Fragments arranged by size:
3319 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
AvaI C'yCGr_G
Cuts at: 1870 1943 2674 2945 3122 1870
Size: 73 731 271 177 4545
Fragments arranged by size:
4545 731 271 177 73
BamHI G'GATC_C
Cuts at: 2499 3127 3494 2499
Size: 628 367 4802
Fragments arranged by size:
4802 628 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2062 2102 2333 2565 2572
Size: 325 217 627 275 40 231 232 7
Cuts at: 2572 3118 4573 618
Size: 546 1455 1842
Fragments arranged by size:
1842 1455 627 546 325 275 232 231
217 40 7
BanII G_rGCy'C
Cuts at: 1391 2015 2099 2496 3024 3116 1391
Size: 624 84 397 528 92 4072
Fragments arranged by size:
4072 624 528 397 92 84
BbeI G_GCGC'C
Cuts at: 2106 2337 2106
Size: 231 5566
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2263 928
Size: 971 364 4462
Fragments arranged by size:
4462 971 364
BbvCI CC'TCA_GC
Cuts at: 2904 3041 2904
Size: 137 5660
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3988 4022 1903
Size: 34 2051 34 3678
Fragments arranged by size:
3678 2051 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3689 4899 3689
Size: 1210 4587
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5797
BfrBI ATG'CAT
Cuts at: 2599 2599
Size: 5797
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2071 2094 4408
Size: 107 122 71 631 605 399 23 2314
Cuts at: 4408 136
Size: 1525
Fragments arranged by size:
2314 1525 631 605 399 122 107 71
23
BglII A'GATC_T
Cuts at: 5792 5792
Size: 5797
BlpI GC'TnA_GC
Cuts at: 2369 2646 3214 2369
Size: 277 568 4952
Fragments arranged by size:
4952 568 277
Bme1580I G_kGCm'C
Cuts at: 1790 2015 2575 2726 3858 5014 1790
Size: 225 560 151 1132 1156 2573
Fragments arranged by size:
2573 1156 1132 560 225 151
BmgBI CAC'GTC
Cuts at: 1318 2286 1318
Size: 968 4829
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3528 4486 442
Size: 416 53 83 399 24 2111 958 1753
Fragments arranged by size:
2111 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2639 3026 3104 4457 1239
Size: 1400 387 78 1353 2579
Fragments arranged by size:
2579 1400 1353 387 78
Bpu10I CC'TnA_GC
Cuts at: 2495 2813 2904 3041 2495
Size: 318 91 137 5251
Fragments arranged by size:
5251 318 137 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2213 3922 4787 5085 2213
Size: 1709 865 298 2925
Fragments arranged by size:
2925 1709 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4460 882
Size: 617 2961 2219
Fragments arranged by size:
2961 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3588 493
Size: 711 2384 2702
Fragments arranged by size:
2702 2384 711
BsaBI GATnn'nnATC
Cuts at: 5791 5791
Size: 5797
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2103 2133 2268 2334
Size: 53 83 186 936 570 30 135 66
Cuts at: 2334 3604 3986 4621 275
Size: 1270 382 635 1451
Fragments arranged by size:
1451 1270 936 635 570 382 186 135
83 66 53 30
BsaWI w'CCGG_w
Cuts at: 4230 4628 4902 5049 5460 4230
Size: 398 274 147 411 4567
Fragments arranged by size:
4567 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5572
Fragments arranged by size:
5572 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2549 2746 3023 1186
Size: 1363 197 277 3960
Fragments arranged by size:
3960 1363 277 197
BseYI C'CCAG_C
Cuts at: 2462 2729 5000 5496 2462
Size: 267 2271 496 2763
Fragments arranged by size:
2763 2271 496 267
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2919 1163
Size: 573 1183 4041
Fragments arranged by size:
4041 1183 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2436 3247 4008 5036 1540
Size: 275 186 435 811 761 1028 2301
Fragments arranged by size:
2301 1028 811 761 435 275 186
BsiHKAI G_wGCw'C
Cuts at: 2382 2496 3024 3858 3943 5014 2382
Size: 114 528 834 85 1071 3165
Fragments arranged by size:
3165 1071 834 528 114 85
BsmI GAATG_Cn'
Cuts at: 3308 3401 3308
Size: 93 5704
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2099 2382 2496 2575 2726 3024
Size: 399 225 84 283 114 79 151 298
Cuts at: 3024 3116 3858 3943 5014 1391
Size: 92 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 298 283 225 151
114 92 85 84 79
BspEI T'CCGG_A
Cuts at: 5460 5460
Size: 5797
BspHI T'CATG_A
Cuts at: 3686 5416 3686
Size: 1730 4067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3085 3152 844
Size: 2241 67 3489
Fragments arranged by size:
3489 2241 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3684 1394
Size: 429 1861 3507
Fragments arranged by size:
3507 1861 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4292 4466 65
Size: 1183 3044 174 1396
Fragments arranged by size:
3044 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2021 2031 2278 2543 4441 4628 1700
Size: 110 211 10 247 265 1898 187 2869
Fragments arranged by size:
2869 1898 265 247 211 187 110 10
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5797
BssHII G'CGCG_C
Cuts at: 1925 2198 2519 1925
Size: 273 321 5203
Fragments arranged by size:
5203 321 273
BssSI C'ACGA_G
Cuts at: 2243 3857 4869 2243
Size: 1614 1012 3171
Fragments arranged by size:
3171 1614 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2074 2083 794
Size: 1280 9 4508
Fragments arranged by size:
4508 1280 9
BstBI TT'CG_AA
Cuts at: 4601 4601
Size: 5797
BstEII G'GTnAC_C
Cuts at: 1184 2317 1184
Size: 1133 4664
BstXI CCAn_nnnn'nTGG
Cuts at: 2329 2470 2842 2329
Size: 141 372 5284
Fragments arranged by size:
5284 372 141
BstYI r'GATC_y
Cuts at: 1972 2499 3127 3494 3879 3896 5348 5434 5446
Size: 527 628 367 385 17 1452 86 12
Cuts at: 5446 5792 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 628 527 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2736 2736
Size: 5797
BtgI C'CryG_G
Cuts at: 513 1065 2628 2827 513
Size: 552 1563 199 3483
Fragments arranged by size:
3483 1563 552 199
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5058
Fragments arranged by size:
5058 683 56
BtsI GCAGTG_nn'
Cuts at: 3313 4107 4127 3313
Size: 794 20 4983
Fragments arranged by size:
4983 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5797
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5762
DraI TTT'AAA
Cuts at: 3101 3101
Size: 5797
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2891 4804 809
Size: 507 1575 1913 1802
Fragments arranged by size:
1913 1802 1575 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2280 2433 2553
Size: 54 1425 50 275 18 450 153 120
Cuts at: 2553 2844 3244 4133 5762 8
Size: 291 400 889 1629 43
Fragments arranged by size:
1629 1425 889 450 400 291 275 153
120 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2433 3244 1537
Size: 275 621 811 4090
Fragments arranged by size:
4090 811 621 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2675 3727 1353
Size: 1322 1052 3423
Fragments arranged by size:
3423 1322 1052
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2543 4384 4758 4904 387
Size: 1064 138 954 1841 374 146 1280
Fragments arranged by size:
1841 1280 1064 954 374 146 138
EcoICRI GAG'CTC
Cuts at: 2494 3022 2494
Size: 528 5269
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2258 2284 2438 2477 2639 2720 2911
Size: 393 626 26 154 39 162 81 191
Cuts at: 2911 3026 3104 3860 4457 5244 5512 1239
Size: 115 78 756 597 787 268 1524
Fragments arranged by size:
1524 787 756 626 597 393 268 191
162 154 115 81 78 39 26
EcoO109I rG'GnC_Cy
Cuts at: 2012 2452 2665 2809 2012
Size: 440 213 144 5000
Fragments arranged by size:
5000 440 213 144
EcoRI G'AATT_C
Cuts at: 3106 3484 3106
Size: 378 5419
EcoRV GAT'ATC
Cuts at: 2620 2620
Size: 5797
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2686 2686
Size: 5797
FspI TGC'GCA
Cuts at: 4303 4303
Size: 5797
HaeII r_GCGC'y
Cuts at: 1678 2106 2337 2369 4944 1678
Size: 428 231 32 2575 2531
Fragments arranged by size:
2575 2531 428 231 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2609 2641 4438 4470 4512
Size: 32 135 32 1037 32 1797 32 42
Cuts at: 4512 4544 1373
Size: 32 2626
Fragments arranged by size:
2626 1797 1037 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3141 3387 669
Size: 988 281 1203 246 3079
Fragments arranged by size:
3079 1203 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5797
HpaI GTT'AAC
Cuts at: 3387 3387
Size: 5797
KasI G'GCGC_C
Cuts at: 2102 2333 2102
Size: 231 5566
KpnI G_GTAC'C
Cuts at: 2569 3122 2569
Size: 553 5244
MfeI C'AATT_G
Cuts at: 3396 3396
Size: 5797
MluI A'CGCG_T
Cuts at: 5673 5673
Size: 5797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4911 5095 1305
Size: 3606 184 2007
Fragments arranged by size:
3606 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2555 2846 10
Size: 54 1425 343 723 291 2961
Fragments arranged by size:
2961 1425 723 343 291 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2327 2714 3755 4114 4273 518
Size: 648 1161 387 1041 359 159 2042
Fragments arranged by size:
2042 1161 1041 648 387 359 159
MspA1I CmG'CkG
Cuts at: 1606 2071 2404 2698 2903 3213 3890 5038 5283
Size: 465 333 294 205 310 677 1148 245
Cuts at: 5283 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 465 333 310 294 245
205
NaeI GCC'GGC
Cuts at: 2023 2280 2023
Size: 257 5540
NarI GG'CG_CC
Cuts at: 2103 2334 2103
Size: 231 5566
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5245
NdeI CA'TA_TG
Cuts at: 387 2971 387
Size: 2584 3213
NgoMIV G'CCGG_C
Cuts at: 2021 2278 2021
Size: 257 5540
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5797
NotI GC'GGCC_GC
Cuts at: 3244 3244
Size: 5797
NsiI A_TGCA'T
Cuts at: 2601 2601
Size: 5797
NspI r_CATG'y
Cuts at: 1132 1229 3155 4700 1132
Size: 97 1926 1545 2229
Fragments arranged by size:
2229 1926 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5797
PciI A'CATG_T
Cuts at: 4696 4696
Size: 5797
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2152 2562 2614 1108
Size: 1044 410 52 4291
Fragments arranged by size:
4291 1044 410 52
PfoI T'CCnGG_A
Cuts at: 2502 2502
Size: 5797
PmeI GTTT'AAAC
Cuts at: 3101 3101
Size: 5797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3866 3898 5379 5411 3866
Size: 32 1481 32 4252
Fragments arranged by size:
4252 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2452 2809 2452
Size: 357 5440
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5797
PsiI TTA'TAA
Cuts at: 3367 3367
Size: 5797
PspOMI G'GGCC_C
Cuts at: 2011 2011
Size: 5797
PstI C_TGCA'G
Cuts at: 830 2079 3149 830
Size: 1249 1070 3478
Fragments arranged by size:
3478 1249 1070
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5797
PvuII CAG'CTG
Cuts at: 2404 2698 2903 2404
Size: 294 205 5298
Fragments arranged by size:
5298 294 205
SacI G_AGCT'C
Cuts at: 2496 3024 2496
Size: 528 5269
SalI G'TCGA_C
Cuts at: 1936 3139 1936
Size: 1203 4594
SbfI CC_TGCA'GG
Cuts at: 3149 3149
Size: 5797
ScaI AGT'ACT
Cuts at: 4045 5757 4045
Size: 1712 4085
SfcI C'TryA_G
Cuts at: 826 1045 2075 3145 4280 4961 5152 826
Size: 219 1030 1070 1135 681 191 1471
Fragments arranged by size:
1471 1135 1070 1030 681 219 191
SfoI GGC'GCC
Cuts at: 2104 2335 2104
Size: 231 5566
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5797
SmaI CCC'GGG
Cuts at: 1872 2676 3124 1872
Size: 804 448 4545
Fragments arranged by size:
4545 804 448
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2228 3901 4802 5064 820
Size: 197 926 285 1673 901 262 1553
Fragments arranged by size:
1673 1553 926 901 285 262 197
SnaBI TAC'GTA
Cuts at: 493 3588 493
Size: 3095 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5797
SphI G_CATG'C
Cuts at: 1132 3155 1132
Size: 2023 3774
SspI AAT'ATT
Cuts at: 5 52 3721 5
Size: 47 3669 2081
Fragments arranged by size:
3669 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5797
StyI C'CwwG_G
Cuts at: 513 1065 2857 2991 3236 513
Size: 552 1792 134 245 3074
Fragments arranged by size:
3074 1792 552 245 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2743 3824 3841 3994 4179 4624 325
Size: 1255 1163 1081 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1163 1081 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2310 2421 4043
Size: 274 80 33 51 1443 333 111 1622
Cuts at: 4043 5755 96
Size: 1712 138
Fragments arranged by size:
1712 1622 1443 333 274 138 111 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5757 5789 5757
Size: 32 5765
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5797
XbaI T'CTAG_A
Cuts at: 3133 3133
Size: 5797
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5797
XmaI C'CCGG_G
Cuts at: 1870 2674 3122 1870
Size: 804 448 4545
Fragments arranged by size:
4545 804 448
XmnI GAAnn'nnTTC
Cuts at: 3926 3926
Size: 5797
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2269 3605 276
Size: 53 83 186 936 735 1336 2468
Fragments arranged by size:
2468 1336 936 735 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BplI BsiWI BsmBI
BstZ17I DraIII EcoNI FseI FspAI NruI PacI PmlI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SrfI SwaI XcmI