Restriction map for
pFN21AB8450
(Circular) (Six-base) MAPSORT of: pFN21AB8450.seq Check: 3638 from: 1 to: 6619
pFN21AB8450 6619 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3619 3619
Size: 6619
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4429 278
Size: 53 83 186 936 2893 2468
Fragments arranged by size:
2893 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3962 1937
Size: 2025 4594
Acc65I G'GTAC_C
Cuts at: 3940 3940
Size: 6619
AclI AA'CG_TT
Cuts at: 1563 4746 5119 1563
Size: 3183 373 3063
Fragments arranged by size:
3183 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2502 2681 3263 4682 6066 6334 1632
Size: 870 179 582 1419 1384 268 1917
Fragments arranged by size:
1917 1419 1384 870 582 268 179
AfeI AGC'GCT
Cuts at: 3512 3512
Size: 6619
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6422
AflIII A'CryG_T
Cuts at: 1203 5518 6495 1203
Size: 4315 977 1327
Fragments arranged by size:
4315 1327 977
AgeI A'CCGG_T
Cuts at: 5450 5450
Size: 6619
AhdI GACnn_n'nnGTC
Cuts at: 5348 5348
Size: 6619
AleI CACnn'nnGTG
Cuts at: 1166 2540 3521 1166
Size: 1374 981 4264
Fragments arranged by size:
4264 1374 981
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3651 3863 1184
Size: 2467 212 3940
Fragments arranged by size:
3940 2467 212
AlwNI CAG_nnn'CTG
Cuts at: 2426 3644 4362 5934 2426
Size: 1218 718 1572 3111
Fragments arranged by size:
3111 1572 1218 718
ApaI G_GGCC'C
Cuts at: 3454 3454
Size: 6619
ApaLI G'TGCA_C
Cuts at: 4676 5832 4676
Size: 1156 5463
ApoI r'AATT_y
Cuts at: 1493 3928 4154 4306 6415 1493
Size: 2435 226 152 2109 1697
Fragments arranged by size:
2435 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5173 160
Size: 872 4141 1606
Fragments arranged by size:
4141 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6619
AvaI C'yCGr_G
Cuts at: 1870 1943 3903 3944 1870
Size: 73 1960 41 4545
Fragments arranged by size:
4545 1960 73 41
BamHI G'GATC_C
Cuts at: 3265 3949 4316 3265
Size: 684 367 5568
Fragments arranged by size:
5568 684 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2395 3940 5395 618
Size: 325 217 627 608 1545 1455 1842
Fragments arranged by size:
1842 1545 1455 627 608 325 217
BanII G_rGCy'C
Cuts at: 1391 2910 3454 3938 1391
Size: 1519 544 484 4072
Fragments arranged by size:
4072 1519 544 484
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5648
BbvCI CC'TCA_GC
Cuts at: 2801 2801
Size: 6619
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4810 4844 1903
Size: 34 2873 34 3678
Fragments arranged by size:
3678 2873 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4511 5721 4511
Size: 1210 5409
BclI T'GATC_A
Cuts at: 1548 2313 1548
Size: 765 5854
BfrBI ATG'CAT
Cuts at: 2333 2333
Size: 6619
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5230 136
Size: 107 122 71 631 605 3558 1525
Fragments arranged by size:
3558 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2976 6614 2976
Size: 3638 2981
BlpI GC'TnA_GC
Cuts at: 4036 4036
Size: 6619
Bme1580I G_kGCm'C
Cuts at: 1790 3239 3454 4680 5836 1790
Size: 1449 215 1226 1156 2573
Fragments arranged by size:
2573 1449 1226 1156 215
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6619
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2206 4350 5308
Size: 416 53 83 399 24 789 2144 958
Cuts at: 5308 442
Size: 1753
Fragments arranged by size:
2144 1753 958 789 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6619
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2431 3832 2431
Size: 1401 5218
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2053 2639 2771 2849 3782 3881 5279 1239
Size: 814 586 132 78 933 99 1398 2579
Fragments arranged by size:
2579 1398 933 814 586 132 99 78
Bpu10I CC'TnA_GC
Cuts at: 2801 2801
Size: 6619
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3155 4744 5609 5907 3155
Size: 1589 865 298 3867
Fragments arranged by size:
3867 1589 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3101 5282 882
Size: 617 1602 2181 2219
Fragments arranged by size:
2219 2181 1602 617
BsaAI yAC'GTr
Cuts at: 493 1204 4410 493
Size: 711 3206 2702
Fragments arranged by size:
3206 2702 711
BsaBI GATnn'nnATC
Cuts at: 2570 6613 2570
Size: 4043 2576
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4426 4808 5443 275
Size: 53 83 186 936 2893 382 635 1451
Fragments arranged by size:
2893 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2118 2562 3314 5052 5450 5724 5871 6282 2118
Size: 444 752 1738 398 274 147 411 2455
Fragments arranged by size:
2455 1738 752 444 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2258 2288 2708 2738 2858
Size: 30 165 30 855 30 420 30 120
Cuts at: 2858 2888 1178
Size: 30 4909
Fragments arranged by size:
4909 855 420 165 120 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2672 2757 2857 3119 3878 1186
Size: 1486 85 100 262 759 3927
Fragments arranged by size:
3927 1486 759 262 100 85
BseYI C'CCAG_C
Cuts at: 2261 2891 3835 5822 6318 2261
Size: 630 944 1987 496 2562
Fragments arranged by size:
2562 1987 944 630 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2366 1163
Size: 573 630 5416
Fragments arranged by size:
5416 630 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3502 4069 4830 5858 1540
Size: 275 186 1501 567 761 1028 2301
Fragments arranged by size:
2301 1501 1028 761 567 275 186
BsiHKAI G_wGCw'C
Cuts at: 2231 3171 4680 4765 5836 2231
Size: 940 1509 85 1071 3014
Fragments arranged by size:
3014 1509 1071 940 85
BsmI GAATG_Cn'
Cuts at: 2135 2785 3782 4130 4223 2135
Size: 650 997 348 93 4531
Fragments arranged by size:
4531 997 650 348 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2728 2839 3572 2728
Size: 111 733 5775
Fragments arranged by size:
5775 733 111
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2231 2910 3171 3239 3454 3938 4680
Size: 399 441 679 261 68 215 484 742
Cuts at: 4680 4765 5836 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 679 484 441 399 261
215 85 68
BspEI T'CCGG_A
Cuts at: 3314 6282 3314
Size: 2968 3651
BspHI T'CATG_A
Cuts at: 3078 4508 6238 3078
Size: 1430 1730 3459
Fragments arranged by size:
3459 1730 1430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3219 3619 3974 844
Size: 2375 400 355 3489
Fragments arranged by size:
3489 2375 400 355
BsrBI CCG'CTC
Cuts at: 1394 1823 2937 4506 1394
Size: 429 1114 1569 3507
Fragments arranged by size:
3507 1569 1114 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2058 5114 5288 65
Size: 1183 810 3056 174 1396
Fragments arranged by size:
3056 1396 1183 810 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2470 5263 5450 1700
Size: 110 660 2793 187 2869
Fragments arranged by size:
2869 2793 660 187 110
BsrGI T'GTAC_A
Cuts at: 96 3189 96
Size: 3093 3526
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6619
BssSI C'ACGA_G
Cuts at: 4679 5691 4679
Size: 1012 5607
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6619
BstBI TT'CG_AA
Cuts at: 5423 5423
Size: 6619
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6619
BstYI r'GATC_y
Cuts at: 1972 2976 3265 3355 3640 3949 4316 4701 4718
Size: 1004 289 90 285 309 367 385 17
Cuts at: 4718 6170 6256 6268 6614 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1004 385 367 346 309 289
285 90 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2855 2855
Size: 6619
BtgI C'CryG_G
Cuts at: 513 1065 2508 3001 3060 3456 513
Size: 552 1443 493 59 396 3676
Fragments arranged by size:
3676 1443 552 493 396 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5880
Fragments arranged by size:
5880 683 56
BtsI GCAGTG_nn'
Cuts at: 4135 4929 4949 4135
Size: 794 20 5805
Fragments arranged by size:
5805 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6619
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6584
DraI TTT'AAA
Cuts at: 3923 3923
Size: 6619
DraIII CAC_nnn'GTG
Cuts at: 3880 3880
Size: 6619
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5626 809
Size: 507 4310 1802
Fragments arranged by size:
4310 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2472 3741 4066
Size: 54 1425 50 275 18 642 1269 325
Cuts at: 4066 4955 6584 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1269 889 642 325 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4066 1537
Size: 275 2254 4090
Fragments arranged by size:
4090 2254 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3432 4549 1353
Size: 2079 1117 3423
Fragments arranged by size:
3423 2079 1117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5206 5580 5726 387
Size: 1064 138 3617 374 146 1280
Fragments arranged by size:
3617 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2053 2502 2639 2681 2771 2849 3263
Size: 393 421 449 137 42 90 78 414
Cuts at: 3263 3782 3881 4682 5279 6066 6334 1239
Size: 519 99 801 597 787 268 1524
Fragments arranged by size:
1524 801 787 597 519 449 421 414
393 268 137 99 90 78 42
EcoNI CCTnn'n_nnAGG
Cuts at: 3017 3017
Size: 6619
EcoO109I rG'GnC_Cy
Cuts at: 2246 2631 3010 3450 3451 3811 2246
Size: 385 379 440 1 360 5054
Fragments arranged by size:
5054 440 385 379 360 1
EcoRI G'AATT_C
Cuts at: 3928 4306 3928
Size: 378 6241
EcoRV GAT'ATC
Cuts at: 3589 3589
Size: 6619
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3043 3043
Size: 6619
FspI TGC'GCA
Cuts at: 3146 3257 3491 5125 3146
Size: 111 234 1634 4640
Fragments arranged by size:
4640 1634 234 111
HaeII r_GCGC'y
Cuts at: 1678 3514 5766 1678
Size: 1836 2252 2531
Fragments arranged by size:
2531 2252 1836
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2708 2740 3347 3379 3578
Size: 32 135 32 1136 32 607 32 199
Cuts at: 3578 3610 5260 5292 5334 5366 1373
Size: 32 1650 32 42 32 2626
Fragments arranged by size:
2626 1650 1136 607 199 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2092 2560 3963 4209 669
Size: 988 281 154 468 1403 246 3079
Fragments arranged by size:
3079 1403 988 468 281 246 154
HindIII A'AGCT_T
Cuts at: 748 3686 748
Size: 2938 3681
HpaI GTT'AAC
Cuts at: 4209 4209
Size: 6619
KpnI G_GTAC'C
Cuts at: 3944 3944
Size: 6619
MfeI C'AATT_G
Cuts at: 4218 4218
Size: 6619
MluI A'CGCG_T
Cuts at: 6495 6495
Size: 6619
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2550 3366 3849 5733 5917 1305
Size: 1245 816 483 1884 184 2007
Fragments arranged by size:
2007 1884 1245 816 483 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4797
Fragments arranged by size:
4797 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2465 2540 3162 3182 3521 4577 4936
Size: 648 1299 75 622 20 339 1056 359
Cuts at: 4936 5095 518
Size: 159 2042
Fragments arranged by size:
2042 1299 1056 648 622 359 339 159
75 20
MspA1I CmG'CkG
Cuts at: 1606 2440 2701 2743 3739 3847 4035 4712 5860
Size: 834 261 42 996 108 188 677 1148
Cuts at: 5860 6105 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 996 834 677 261 245 188
108 42
NaeI GCC'GGC
Cuts at: 2472 2472
Size: 6619
NcoI C'CATG_G
Cuts at: 513 1065 3001 3456 513
Size: 552 1936 455 3676
Fragments arranged by size:
3676 1936 552 455
NdeI CA'TA_TG
Cuts at: 387 2335 387
Size: 1948 4671
NgoMIV G'CCGG_C
Cuts at: 2470 2470
Size: 6619
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6619
NotI GC'GGCC_GC
Cuts at: 4066 4066
Size: 6619
NsiI A_TGCA'T
Cuts at: 2335 2335
Size: 6619
NspI r_CATG'y
Cuts at: 1132 1229 2354 2776 3889 3977 5522 1132
Size: 97 1125 422 1113 88 1545 2229
Fragments arranged by size:
2229 1545 1125 1113 422 97 88
PasI CC'CwG_GG
Cuts at: 1348 2603 1348
Size: 1255 5364
PciI A'CATG_T
Cuts at: 5518 5518
Size: 6619
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2048 2734 1108
Size: 940 686 4993
Fragments arranged by size:
4993 940 686
PfoI T'CCnGG_A
Cuts at: 2616 2616
Size: 6619
PmeI GTTT'AAAC
Cuts at: 3923 3923
Size: 6619
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2833 2865 4688 4720 6201 6233 2833
Size: 32 1823 32 1481 32 3219
Fragments arranged by size:
3219 1823 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2631 3010 2631
Size: 379 6240
PshAI GACnn'nnGTC
Cuts at: 1613 2636 1613
Size: 1023 5596
PsiI TTA'TAA
Cuts at: 4189 4189
Size: 6619
PspOMI G'GGCC_C
Cuts at: 3450 3450
Size: 6619
PstI C_TGCA'G
Cuts at: 830 3758 3856 3971 830
Size: 2928 98 115 3478
Fragments arranged by size:
3478 2928 115 98
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6619
PvuII CAG'CTG
Cuts at: 2440 2701 2743 2440
Size: 261 42 6316
Fragments arranged by size:
6316 261 42
SalI G'TCGA_C
Cuts at: 1936 3961 1936
Size: 2025 4594
SanDI GG'GwC_CC
Cuts at: 2631 2631
Size: 6619
SbfI CC_TGCA'GG
Cuts at: 3971 3971
Size: 6619
ScaI AGT'ACT
Cuts at: 4867 6579 4867
Size: 1712 4907
SfcI C'TryA_G
Cuts at: 826 1045 3754 3852 3967 5102 5783 5974 826
Size: 219 2709 98 115 1135 681 191 1471
Fragments arranged by size:
2709 1471 1135 681 219 191 115 98
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6619
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6619
SmaI CCC'GGG
Cuts at: 1872 3905 3946 1872
Size: 2033 41 4545
Fragments arranged by size:
4545 2033 41
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3170 4723 5624 5886 820
Size: 197 926 1227 1553 901 262 1553
Fragments arranged by size:
1553 1553 1227 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4410 493
Size: 3917 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6619
SphI G_CATG'C
Cuts at: 1132 2354 3889 3977 1132
Size: 1222 1535 88 3774
Fragments arranged by size:
3774 1535 1222 88
SspI AAT'ATT
Cuts at: 5 52 4543 5
Size: 47 4491 2081
Fragments arranged by size:
4491 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6619
StyI C'CwwG_G
Cuts at: 513 1065 2187 2817 3001 3456 4058 513
Size: 552 1122 630 184 455 602 3074
Fragments arranged by size:
3074 1122 630 602 552 455 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2214 2295 2413 4646 4663 4816 5001
Size: 1255 634 81 118 2233 17 153 185
Cuts at: 5001 5446 325
Size: 445 1498
Fragments arranged by size:
2233 1498 1255 634 445 185 153 118
81 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3189 3222 4865
Size: 274 80 33 51 1443 1212 33 1643
Cuts at: 4865 6577 96
Size: 1712 138
Fragments arranged by size:
1712 1643 1443 1212 274 138 80 51
33 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3703 3735 6579 6611 3703
Size: 32 2844 32 3711
Fragments arranged by size:
3711 2844 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2720 1316
Size: 1404 5215
XbaI T'CTAG_A
Cuts at: 3955 3955
Size: 6619
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2073 2824 2011
Size: 62 751 5806
Fragments arranged by size:
5806 751 62
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6619
XmaI C'CCGG_G
Cuts at: 1870 3903 3944 1870
Size: 2033 41 4545
Fragments arranged by size:
4545 2033 41
XmnI GAAnn'nnTTC
Cuts at: 2946 3023 3689 4748 2946
Size: 77 666 1059 4817
Fragments arranged by size:
4817 1059 666 77
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4427 276
Size: 53 83 186 936 2893 2468
Fragments arranged by size:
2893 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BsiWI BstXI BstZ17I
EcoICRI FseI FspAI KasI NarI NruI PacI PmlI
PspXI PsrI RsrII SacI SacII SapI SexAI SfiI
SfoI SrfI SwaI