Restriction map for
pFN21AB8446
(Circular) (Six-base) MAPSORT of: pFN21AB8446.seq Check: 8451 from: 1 to: 5632
pFN21AB8446 5632 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3442 278
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2975 1937
Size: 1038 4594
Acc65I G'GTAC_C
Cuts at: 2953 2953
Size: 5632
AclI AA'CG_TT
Cuts at: 1563 3759 4132 1563
Size: 2196 373 3063
Fragments arranged by size:
3063 2196 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3695 5079 5347 1632
Size: 2063 1384 268 1917
Fragments arranged by size:
2063 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2153 820
Size: 197 1136 4299
Fragments arranged by size:
4299 1136 197
AflIII A'CryG_T
Cuts at: 1203 4531 5508 1203
Size: 3328 977 1327
Fragments arranged by size:
3328 1327 977
AgeI A'CCGG_T
Cuts at: 4463 4463
Size: 5632
AhdI GACnn_n'nnGTC
Cuts at: 4361 4361
Size: 5632
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5632
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5632
AlwNI CAG_nnn'CTG
Cuts at: 2401 3375 4947 2401
Size: 974 1572 3086
Fragments arranged by size:
3086 1572 974
ApaI G_GGCC'C
Cuts at: 2595 2595
Size: 5632
ApaLI G'TGCA_C
Cuts at: 3689 4845 3689
Size: 1156 4476
ApoI r'AATT_y
Cuts at: 1493 2126 2219 2258 2941 3167 3319 5428 1493
Size: 633 93 39 683 226 152 2109 1697
Fragments arranged by size:
2109 1697 683 633 226 152 93 39
AseI AT'TA_AT
Cuts at: 160 1032 4186 160
Size: 872 3154 1606
Fragments arranged by size:
3154 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
AvaI C'yCGr_G
Cuts at: 1870 1943 2957 1870
Size: 73 1014 4545
Fragments arranged by size:
4545 1014 73
BamHI G'GATC_C
Cuts at: 2962 3329 2962
Size: 367 5265
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2953 4408 618
Size: 325 217 627 1166 1455 1842
Fragments arranged by size:
1842 1455 1166 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2595 2752 2812 2951 1391
Size: 1204 157 60 139 4072
Fragments arranged by size:
4072 1204 157 139 60
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4661
BbvCI CC'TCA_GC
Cuts at: 2687 2828 2687
Size: 141 5491
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3823 3857 1903
Size: 34 1886 34 3678
Fragments arranged by size:
3678 1886 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3524 4734 3524
Size: 1210 4422
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5632
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4243 136
Size: 107 122 71 631 605 2571 1525
Fragments arranged by size:
2571 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2161 5627 2161
Size: 3466 2166
BlpI GC'TnA_GC
Cuts at: 3049 3049
Size: 5632
Bme1580I G_kGCm'C
Cuts at: 1790 2595 3693 4849 1790
Size: 805 1098 1156 2573
Fragments arranged by size:
2573 1156 1098 805
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5632
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2734 3363 4321
Size: 416 53 83 399 24 1317 629 958
Cuts at: 4321 442
Size: 1753
Fragments arranged by size:
1753 1317 958 629 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2378 2663 2678 4292 1239
Size: 1139 285 15 1614 2579
Fragments arranged by size:
2579 1614 1139 285 15
Bpu10I CC'TnA_GC
Cuts at: 2687 2828 2687
Size: 141 5491
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3757 4622 4920 3757
Size: 865 298 4469
Fragments arranged by size:
4469 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2709 4295 882
Size: 617 1210 1586 2219
Fragments arranged by size:
2219 1586 1210 617
BsaAI yAC'GTr
Cuts at: 493 1204 3423 493
Size: 711 2219 2702
Fragments arranged by size:
2702 2219 711
BsaBI GATnn'nnATC
Cuts at: 5626 5626
Size: 5632
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3439 3821 4456 275
Size: 53 83 186 936 1906 382 635 1451
Fragments arranged by size:
1906 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2043 4065 4463 4737 4884 5295 2043
Size: 2022 398 274 147 411 2380
Fragments arranged by size:
2380 2022 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2540 2570 1178
Size: 30 165 30 1137 30 4240
Fragments arranged by size:
4240 1137 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5632
BseYI C'CCAG_C
Cuts at: 4835 5331 4835
Size: 496 5136
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5059
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3082 3843 4871 1540
Size: 275 186 1081 761 1028 2301
Fragments arranged by size:
2301 1081 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2812 3693 3778 4849 2812
Size: 881 85 1071 3595
Fragments arranged by size:
3595 1071 881 85
BsmI GAATG_Cn'
Cuts at: 2217 3143 3236 2217
Size: 926 93 4613
Fragments arranged by size:
4613 926 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2595 2752 2812 2951 3693 3778 4849
Size: 399 805 157 60 139 742 85 1071
Cuts at: 4849 1391
Size: 2174
Fragments arranged by size:
2174 1071 805 742 399 157 139 85
60
BspEI T'CCGG_A
Cuts at: 5295 5295
Size: 5632
BspHI T'CATG_A
Cuts at: 3521 5251 3521
Size: 1730 3902
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2559 2808 2987 844
Size: 1715 249 179 3489
Fragments arranged by size:
3489 1715 249 179
BsrBI CCG'CTC
Cuts at: 1394 1823 2773 3519 1394
Size: 429 950 746 3507
Fragments arranged by size:
3507 950 746 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4127 4301 65
Size: 1183 2879 174 1396
Fragments arranged by size:
2879 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2730 4276 4463 1700
Size: 110 920 1546 187 2869
Fragments arranged by size:
2869 1546 920 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5632
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5632
BssSI C'ACGA_G
Cuts at: 3692 4704 3692
Size: 1012 4620
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2429 2851 794
Size: 1635 422 3575
Fragments arranged by size:
3575 1635 422
BstBI TT'CG_AA
Cuts at: 4436 4436
Size: 5632
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5632
BstXI CCAn_nnnn'nTGG
Cuts at: 2587 2587
Size: 5632
BstYI r'GATC_y
Cuts at: 1972 2161 2649 2962 3329 3714 3731 5183 5269
Size: 189 488 313 367 385 17 1452 86
Cuts at: 5269 5281 5627 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 488 385 367 346 313 189
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2892 513
Size: 552 1827 3253
Fragments arranged by size:
3253 1827 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4893
Fragments arranged by size:
4893 683 56
BtsI GCAGTG_nn'
Cuts at: 2786 3148 3942 3962 2786
Size: 362 794 20 4456
Fragments arranged by size:
4456 794 362 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5632
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5597
DraI TTT'AAA
Cuts at: 2238 2936 2238
Size: 698 4934
DraIII CAC_nnn'GTG
Cuts at: 2602 2602
Size: 5632
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4639 809
Size: 507 3323 1802
Fragments arranged by size:
3323 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2835 3079 3968
Size: 54 1425 50 275 18 1005 244 889
Cuts at: 3968 5597 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1005 889 275 244 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3079 1537
Size: 275 1267 4090
Fragments arranged by size:
4090 1267 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3562 1353
Size: 2209 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2642 2749 4219 4593 4739 387
Size: 1064 138 1053 107 1470 374 146 1280
Fragments arranged by size:
1470 1280 1064 1053 374 146 138 107
EcoICRI GAG'CTC
Cuts at: 2810 2810
Size: 5632
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2378 2663 2678 3695 4292 5079 5347
Size: 393 746 285 15 1017 597 787 268
Cuts at: 5347 1239
Size: 1524
Fragments arranged by size:
1524 1017 787 746 597 393 285 268
15
EcoNI CCTnn'n_nnAGG
Cuts at: 2837 2837
Size: 5632
EcoO109I rG'GnC_Cy
Cuts at: 2344 2548 2591 2592 2344
Size: 204 43 1 5384
Fragments arranged by size:
5384 204 43 1
EcoRI G'AATT_C
Cuts at: 2941 3319 2941
Size: 378 5254
EcoRV GAT'ATC
Cuts at: 2205 2205
Size: 5632
FspI TGC'GCA
Cuts at: 2338 2804 4138 2338
Size: 466 1334 3832
Fragments arranged by size:
3832 1334 466
HaeII r_GCGC'y
Cuts at: 1678 2710 4779 1678
Size: 1032 2069 2531
Fragments arranged by size:
2531 2069 1032
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2540 2572 4273 4305 4347
Size: 32 135 32 968 32 1701 32 42
Cuts at: 4347 4379 1373
Size: 32 2626
Fragments arranged by size:
2626 1701 968 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2976 3222 669
Size: 988 281 1038 246 3079
Fragments arranged by size:
3079 1038 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5632
HpaI GTT'AAC
Cuts at: 3222 3222
Size: 5632
KpnI G_GTAC'C
Cuts at: 2957 2957
Size: 5632
MfeI C'AATT_G
Cuts at: 3231 3231
Size: 5632
MluI A'CGCG_T
Cuts at: 5508 5508
Size: 5632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2116 2163 4746 4930 1305
Size: 811 47 2583 184 2007
Fragments arranged by size:
2583 2007 811 184 47
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2837 10
Size: 54 1425 343 1005 2805
Fragments arranged by size:
2805 1425 1005 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3590 3949 4108 518
Size: 648 2424 359 159 2042
Fragments arranged by size:
2424 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2616 2641 3048 3725 4873 5118 1606
Size: 1010 25 407 677 1148 245 2120
Fragments arranged by size:
2120 1148 1010 677 407 245 25
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5080
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5632
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5632
NotI GC'GGCC_GC
Cuts at: 3079 3079
Size: 5632
NspI r_CATG'y
Cuts at: 1132 1229 2990 4535 1132
Size: 97 1761 1545 2229
Fragments arranged by size:
2229 1761 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5632
PciI A'CATG_T
Cuts at: 4531 4531
Size: 5632
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2401 2602 1108
Size: 1293 201 4138
Fragments arranged by size:
4138 1293 201
PfoI T'CCnGG_A
Cuts at: 2676 2694 2676
Size: 18 5614
PmeI GTTT'AAAC
Cuts at: 2936 2936
Size: 5632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3701 3733 5214 5246 3701
Size: 32 1481 32 4087
Fragments arranged by size:
4087 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2548 2548
Size: 5632
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5632
PsiI TTA'TAA
Cuts at: 3202 3202
Size: 5632
PspOMI G'GGCC_C
Cuts at: 2591 2591
Size: 5632
PstI C_TGCA'G
Cuts at: 830 2370 2984 830
Size: 1540 614 3478
Fragments arranged by size:
3478 1540 614
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5632
PvuII CAG'CTG
Cuts at: 2641 2641
Size: 5632
SacI G_AGCT'C
Cuts at: 2812 2812
Size: 5632
SalI G'TCGA_C
Cuts at: 1936 2974 1936
Size: 1038 4594
SbfI CC_TGCA'GG
Cuts at: 2984 2984
Size: 5632
ScaI AGT'ACT
Cuts at: 3880 5592 3880
Size: 1712 3920
SfcI C'TryA_G
Cuts at: 826 1045 2071 2366 2980 4115 4796 4987 826
Size: 219 1026 295 614 1135 681 191 1471
Fragments arranged by size:
1471 1135 1026 681 614 295 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5632
SmaI CCC'GGG
Cuts at: 1872 2959 1872
Size: 1087 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2153 3736 4637 4899 820
Size: 197 926 210 1583 901 262 1553
Fragments arranged by size:
1583 1553 926 901 262 210 197
SnaBI TAC'GTA
Cuts at: 493 3423 493
Size: 2930 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5632
SphI G_CATG'C
Cuts at: 1132 2990 1132
Size: 1858 3774
SspI AAT'ATT
Cuts at: 5 52 3556 5
Size: 47 3504 2081
Fragments arranged by size:
3504 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5632
StyI C'CwwG_G
Cuts at: 513 1065 2586 2838 3071 513
Size: 552 1521 252 233 3074
Fragments arranged by size:
3074 1521 552 252 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3659 3676 3829 4014 4459 325
Size: 1255 2079 17 153 185 445 1498
Fragments arranged by size:
2079 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3878 5590 96
Size: 274 80 33 51 1443 1901 1712 138
Fragments arranged by size:
1901 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5592 5624 5592
Size: 32 5600
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5632
XbaI T'CTAG_A
Cuts at: 2968 2968
Size: 5632
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2470 2470
Size: 5632
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5632
XmaI C'CCGG_G
Cuts at: 1870 2957 1870
Size: 1087 4545
XmnI GAAnn'nnTTC
Cuts at: 2258 2444 3761 2258
Size: 186 1317 4129
Fragments arranged by size:
4129 1317 186
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3440 276
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI