Restriction map for
pFN21AB8435
(Circular) (Six-base) MAPSORT of: pFN21AB8435.seq Check: 25 from: 1 to: 6214
pFN21AB8435 6214 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4024 278
Size: 53 83 186 936 2488 2468
Fragments arranged by size:
2488 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3557 1937
Size: 1620 4594
Acc65I G'GTAC_C
Cuts at: 3535 3535
Size: 6214
AclI AA'CG_TT
Cuts at: 1563 4341 4714 1563
Size: 2778 373 3063
Fragments arranged by size:
3063 2778 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2228 2754 2825 3246 4277 5661 5929 1632
Size: 596 526 71 421 1031 1384 268 1917
Fragments arranged by size:
1917 1384 1031 596 526 421 268 71
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6017
AflIII A'CryG_T
Cuts at: 1203 3331 5113 6090 1203
Size: 2128 1782 977 1327
Fragments arranged by size:
2128 1782 1327 977
AgeI A'CCGG_T
Cuts at: 5045 5045
Size: 6214
AhdI GACnn_n'nnGTC
Cuts at: 2096 2704 4943 2096
Size: 608 2239 3367
Fragments arranged by size:
3367 2239 608
AleI CACnn'nnGTG
Cuts at: 1166 2015 1166
Size: 849 5365
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6214
AlwNI CAG_nnn'CTG
Cuts at: 2176 2349 2365 2761 3957 5529 2176
Size: 173 16 396 1196 1572 2861
Fragments arranged by size:
2861 1572 1196 396 173 16
ApaLI G'TGCA_C
Cuts at: 3335 3473 4271 5427 3335
Size: 138 798 1156 4122
Fragments arranged by size:
4122 1156 798 138
ApoI r'AATT_y
Cuts at: 1493 2941 3523 3749 3901 6010 1493
Size: 1448 582 226 152 2109 1697
Fragments arranged by size:
2109 1697 1448 582 226 152
AscI GG'CGCG_CC
Cuts at: 3492 3492
Size: 6214
AseI AT'TA_AT
Cuts at: 160 1032 4768 160
Size: 872 3736 1606
Fragments arranged by size:
3736 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
AvaI C'yCGr_G
Cuts at: 1870 1943 3487 3539 1870
Size: 73 1544 52 4545
Fragments arranged by size:
4545 1544 73 52
BamHI G'GATC_C
Cuts at: 3544 3911 3544
Size: 367 5847
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2690 3444 3535 4990 618
Size: 325 217 627 903 754 91 1455 1842
Fragments arranged by size:
1842 1455 903 754 627 325 217 91
BanII G_rGCy'C
Cuts at: 1391 2298 3533 1391
Size: 907 1235 4072
Fragments arranged by size:
4072 1235 907
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2222 2263 2310 3060 3108 928
Size: 971 323 41 47 750 48 4034
Fragments arranged by size:
4034 971 750 323 48 47 41
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2321 2355 4405 4439 1903
Size: 34 384 34 2050 34 3678
Fragments arranged by size:
3678 2050 384 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2363 4106 5316 2363
Size: 1743 1210 3261
Fragments arranged by size:
3261 1743 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6214
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2798 3456 4825
Size: 107 122 71 631 605 1126 658 1369
Cuts at: 4825 136
Size: 1525
Fragments arranged by size:
1525 1369 1126 658 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6209 6209
Size: 6214
BlpI GC'TnA_GC
Cuts at: 3631 3631
Size: 6214
Bme1580I G_kGCm'C
Cuts at: 1790 3339 3477 4275 5431 1790
Size: 1549 138 798 1156 2573
Fragments arranged by size:
2573 1549 1156 798 138
BmgBI CAC'GTC
Cuts at: 1318 3340 1318
Size: 2022 4192
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3242 3945 4903
Size: 416 53 83 399 24 1825 703 958
Cuts at: 4903 442
Size: 1753
Fragments arranged by size:
1825 1753 958 703 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6214
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2205 2933 2205
Size: 728 5486
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2198 2387 3083 4874 1239
Size: 959 189 696 1791 2579
Fragments arranged by size:
2579 1791 959 696 189
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2399 2888 4339 5204 5502 2399
Size: 489 1451 865 298 3111
Fragments arranged by size:
3111 1451 865 489 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2403 3425 4877 882
Size: 617 904 1022 1452 2219
Fragments arranged by size:
2219 1452 1022 904 617
BsaAI yAC'GTr
Cuts at: 493 1204 3334 4005 493
Size: 711 2130 671 2702
Fragments arranged by size:
2702 2130 711 671
BsaBI GATnn'nnATC
Cuts at: 6208 6208
Size: 6214
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4021 4403 5038 275
Size: 53 83 186 936 2488 382 635 1451
Fragments arranged by size:
2488 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4647 5045 5319 5466 5877 4647
Size: 398 274 147 411 4984
Fragments arranged by size:
4984 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2228 2258 2713 2743 3034
Size: 30 165 30 825 30 455 30 291
Cuts at: 3034 3064 1178
Size: 30 4328
Fragments arranged by size:
4328 825 455 291 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2264 3443 1186
Size: 1078 1179 3957
Fragments arranged by size:
3957 1179 1078
BseYI C'CCAG_C
Cuts at: 5417 5913 5417
Size: 496 5718
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2008 2092 2516 3039 1163
Size: 573 272 84 424 523 4338
Fragments arranged by size:
4338 573 523 424 272 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3664 4425 5453 1540
Size: 275 186 1663 761 1028 2301
Fragments arranged by size:
2301 1663 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2298 3339 3477 4275 4360 5431 2298
Size: 1041 138 798 85 1071 3081
Fragments arranged by size:
3081 1071 1041 798 138 85
BsmI GAATG_Cn'
Cuts at: 2212 3725 3818 2212
Size: 1513 93 4608
Fragments arranged by size:
4608 1513 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2298 3339 3477 3533 4275 4360 5431
Size: 399 508 1041 138 56 742 85 1071
Cuts at: 5431 1391
Size: 2174
Fragments arranged by size:
2174 1071 1041 742 508 399 138 85
56
BspEI T'CCGG_A
Cuts at: 5877 5877
Size: 6214
BspHI T'CATG_A
Cuts at: 4103 5833 4103
Size: 1730 4484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2030 2436 3291 3502 3569 844
Size: 1186 406 855 211 67 3489
Fragments arranged by size:
3489 1186 855 406 211 67
BsrBI CCG'CTC
Cuts at: 1394 1823 4101 1394
Size: 429 2278 3507
Fragments arranged by size:
3507 2278 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4709 4883 65
Size: 1183 3461 174 1396
Fragments arranged by size:
3461 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2111 3405 4858 5045 1700
Size: 110 301 1294 1453 187 2869
Fragments arranged by size:
2869 1453 1294 301 187 110
BsrGI T'GTAC_A
Cuts at: 96 2616 96
Size: 2520 3694
BssHII G'CGCG_C
Cuts at: 1925 3492 1925
Size: 1567 4647
BssSI C'ACGA_G
Cuts at: 2627 4274 5286 2627
Size: 1647 1012 3555
Fragments arranged by size:
3555 1647 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3472 794
Size: 2678 3536
BstBI TT'CG_AA
Cuts at: 5018 5018
Size: 6214
BstEII G'GTnAC_C
Cuts at: 1184 2017 1184
Size: 833 5381
BstXI CCAn_nnnn'nTGG
Cuts at: 2646 2929 2646
Size: 283 5931
BstYI r'GATC_y
Cuts at: 1972 2193 2361 3544 3911 4296 4313 5765 5851
Size: 221 168 1183 367 385 17 1452 86
Cuts at: 5851 5863 6209 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1183 385 367 346 221 168
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2553 2553
Size: 6214
BtgI C'CryG_G
Cuts at: 513 1065 2004 2013 2813 2832 3138 513
Size: 552 939 9 800 19 306 3589
Fragments arranged by size:
3589 939 800 552 306 19 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3349 487
Size: 683 56 2123 3352
Fragments arranged by size:
3352 2123 683 56
BtsI GCAGTG_nn'
Cuts at: 2466 3481 3730 4524 4544 2466
Size: 1015 249 794 20 4136
Fragments arranged by size:
4136 1015 794 249 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6214
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6179
DraI TTT'AAA
Cuts at: 3518 3518
Size: 6214
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5221 809
Size: 507 3905 1802
Fragments arranged by size:
3905 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2113 2436
Size: 54 1425 50 275 18 177 106 323
Cuts at: 2436 2931 3288 3661 4550 6179 8
Size: 495 357 373 889 1629 43
Fragments arranged by size:
1629 1425 889 495 373 357 323 275
177 106 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3661 1537
Size: 275 1849 4090
Fragments arranged by size:
4090 1849 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4144 1353
Size: 2791 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4801 5175 5321 387
Size: 1064 138 3212 374 146 1280
Fragments arranged by size:
3212 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2296 2296
Size: 6214
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 2228 2387 2754 2825 3083 3246
Size: 393 566 30 159 367 71 258 163
Cuts at: 3246 4277 4874 5661 5929 1239
Size: 1031 597 787 268 1524
Fragments arranged by size:
1524 1031 787 597 566 393 367 268
258 163 159 71 30
EcoO109I rG'GnC_Cy
Cuts at: 2557 2800 2557
Size: 243 5971
EcoRI G'AATT_C
Cuts at: 3523 3901 3523
Size: 378 5836
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3062 3062
Size: 6214
FseI GG_CCGG'CC
Cuts at: 3409 3409
Size: 6214
FspI TGC'GCA
Cuts at: 4720 4720
Size: 6214
HaeII r_GCGC'y
Cuts at: 1678 5361 1678
Size: 3683 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2082 2114 2228 2260 2690
Size: 32 135 32 510 32 114 32 430
Cuts at: 2690 2722 3034 3066 4855 4887 4929 4961 1373
Size: 32 312 32 1789 32 42 32 2626
Fragments arranged by size:
2626 1789 510 430 312 135 114 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3163 3558 3804 669
Size: 988 281 1225 395 246 3079
Fragments arranged by size:
3079 1225 988 395 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6214
HpaI GTT'AAC
Cuts at: 3804 3804
Size: 6214
KpnI G_GTAC'C
Cuts at: 3539 3539
Size: 6214
MfeI C'AATT_G
Cuts at: 3813 3813
Size: 6214
MluI A'CGCG_T
Cuts at: 6090 6090
Size: 6214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2113 2241 5328 5512 1305
Size: 808 128 3087 184 2007
Fragments arranged by size:
3087 2007 808 184 128
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2438 2933 3290 10
Size: 54 1425 343 177 429 495 357 2934
Fragments arranged by size:
2934 1425 495 429 357 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2015 2121 2924 4172 4531 4690 518
Size: 648 849 106 803 1248 359 159 2042
Fragments arranged by size:
2042 1248 849 803 648 359 159 106
MspA1I CmG'CkG
Cuts at: 1606 3508 3630 4307 5455 5700 1606
Size: 1902 122 677 1148 245 2120
Fragments arranged by size:
2120 1902 1148 677 245 122
NaeI GCC'GGC
Cuts at: 3407 3407
Size: 6214
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4723
Fragments arranged by size:
4723 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6214
NgoMIV G'CCGG_C
Cuts at: 3405 3405
Size: 6214
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6214
NotI GC'GGCC_GC
Cuts at: 3661 3661
Size: 6214
NspI r_CATG'y
Cuts at: 1132 1229 2604 3572 5117 1132
Size: 97 1375 968 1545 2229
Fragments arranged by size:
2229 1545 1375 968 97
PasI CC'CwG_GG
Cuts at: 1348 3203 1348
Size: 1855 4359
PciI A'CATG_T
Cuts at: 5113 5113
Size: 6214
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2176 2266 1108
Size: 1068 90 5056
Fragments arranged by size:
5056 1068 90
PfoI T'CCnGG_A
Cuts at: 2364 2364
Size: 6214
PmeI GTTT'AAAC
Cuts at: 3518 3518
Size: 6214
PmlI CAC'GTG
Cuts at: 3334 3334
Size: 6214
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4283 4315 5796 5828 4283
Size: 32 1481 32 4669
Fragments arranged by size:
4669 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2557 2557
Size: 6214
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6214
PsiI TTA'TAA
Cuts at: 3784 3784
Size: 6214
PstI C_TGCA'G
Cuts at: 830 2461 3513 3566 830
Size: 1631 1052 53 3478
Fragments arranged by size:
3478 1631 1052 53
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6214
SacI G_AGCT'C
Cuts at: 2298 2298
Size: 6214
SalI G'TCGA_C
Cuts at: 1936 3556 1936
Size: 1620 4594
SbfI CC_TGCA'GG
Cuts at: 3566 3566
Size: 6214
ScaI AGT'ACT
Cuts at: 2324 4462 6174 2324
Size: 2138 1712 2364
Fragments arranged by size:
2364 2138 1712
SexAI A'CCwGG_T
Cuts at: 2265 3240 2265
Size: 975 5239
SfcI C'TryA_G
Cuts at: 826 1045 2326 2457 3509 3562 4697 5378 5569
Size: 219 1281 131 1052 53 1135 681 191
Cuts at: 5569 826
Size: 1471
Fragments arranged by size:
1471 1281 1135 1052 681 219 191 131
53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6214
SmaI CCC'GGG
Cuts at: 1872 3541 1872
Size: 1669 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2414 2903 4318 5219 5481 820
Size: 197 926 471 489 1415 901 262 1553
Fragments arranged by size:
1553 1415 926 901 489 471 262 197
SnaBI TAC'GTA
Cuts at: 493 4005 493
Size: 3512 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6214
SphI G_CATG'C
Cuts at: 1132 3572 1132
Size: 2440 3774
SspI AAT'ATT
Cuts at: 5 52 4138 5
Size: 47 4086 2081
Fragments arranged by size:
4086 2081 47
StuI AGG'CCT
Cuts at: 1517 3182 1517
Size: 1665 4549
StyI C'CwwG_G
Cuts at: 513 1065 2004 3653 513
Size: 552 939 1649 3074
Fragments arranged by size:
3074 1649 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4241 4258 4411 4596 5041 325
Size: 1255 2661 17 153 185 445 1498
Fragments arranged by size:
2661 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2322 2616 3387
Size: 274 80 33 51 1443 345 294 771
Cuts at: 3387 4460 6172 96
Size: 1073 1712 138
Fragments arranged by size:
1712 1443 1073 771 345 294 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2713 2745 6174 6206 2713
Size: 32 3429 32 2721
Fragments arranged by size:
3429 2721 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2723 1316
Size: 1407 4807
XbaI T'CTAG_A
Cuts at: 3550 3550
Size: 6214
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6214
XmaI C'CCGG_G
Cuts at: 1870 3539 1870
Size: 1669 4545
XmnI GAAnn'nnTTC
Cuts at: 2882 4343 2882
Size: 1461 4753
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4022 276
Size: 53 83 186 936 2488 2468
Fragments arranged by size:
2488 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV
FspAI KasI NarI NruI NsiI PacI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI
SrfI SwaI XcmI