Restriction map for
pFN21AB8433
(Circular) (Six-base) MAPSORT of: pFN21AB8433.seq Check: 5499 from: 1 to: 5554
pFN21AB8433 5554 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2218 2218
Size: 5554
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3364 278
Size: 53 83 186 936 1828 2468
Fragments arranged by size:
2468 1828 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2897 1937
Size: 960 4594
Acc65I G'GTAC_C
Cuts at: 2400 2875 2400
Size: 475 5079
AclI AA'CG_TT
Cuts at: 1563 3681 4054 1563
Size: 2118 373 3063
Fragments arranged by size:
3063 2118 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2114 2303 2666 3617 5001 5269 1632
Size: 482 189 363 951 1384 268 1917
Fragments arranged by size:
1917 1384 951 482 363 268 189
AfeI AGC'GCT
Cuts at: 2843 2843
Size: 5554
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5357
AflIII A'CryG_T
Cuts at: 1203 2454 4453 5430 1203
Size: 1251 1999 977 1327
Fragments arranged by size:
1999 1327 1251 977
AgeI A'CCGG_T
Cuts at: 4385 4385
Size: 5554
AhdI GACnn_n'nnGTC
Cuts at: 4283 4283
Size: 5554
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5554
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2240 1184
Size: 1056 4498
AlwNI CAG_nnn'CTG
Cuts at: 2161 2251 2554 3297 4869 2161
Size: 90 303 743 1572 2846
Fragments arranged by size:
2846 1572 743 303 90
ApaLI G'TGCA_C
Cuts at: 3611 4767 3611
Size: 1156 4398
ApoI r'AATT_y
Cuts at: 1493 2863 3089 3241 5350 1493
Size: 1370 226 152 2109 1697
Fragments arranged by size:
2109 1697 1370 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4108 160
Size: 872 3076 1606
Fragments arranged by size:
3076 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5554
AvaI C'yCGr_G
Cuts at: 1870 1943 2346 2879 1870
Size: 73 403 533 4545
Fragments arranged by size:
4545 533 403 73
BamHI G'GATC_C
Cuts at: 2884 3251 2884
Size: 367 5187
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2400 2875 4330 618
Size: 325 217 627 613 475 1455 1842
Fragments arranged by size:
1842 1455 627 613 475 325 217
BanII G_rGCy'C
Cuts at: 1391 2410 2735 2775 2873 1391
Size: 1019 325 40 98 4072
Fragments arranged by size:
4072 1019 325 98 40
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2330 928
Size: 971 431 4152
Fragments arranged by size:
4152 971 431
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3745 3779 1903
Size: 34 1808 34 3678
Fragments arranged by size:
3678 1808 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3446 4656 3446
Size: 1210 4344
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5554
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4165 136
Size: 107 122 71 631 605 2493 1525
Fragments arranged by size:
2493 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5549 5549
Size: 5554
BlpI GC'TnA_GC
Cuts at: 2971 2971
Size: 5554
Bme1580I G_kGCm'C
Cuts at: 1790 2561 3615 4771 1790
Size: 771 1054 1156 2573
Fragments arranged by size:
2573 1156 1054 771
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5554
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2357 2559 3285
Size: 416 53 83 399 24 940 202 726
Cuts at: 3285 4243 442
Size: 958 1753
Fragments arranged by size:
1753 958 940 726 416 399 202 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5554
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2141 2195 2228 2747 4214 1239
Size: 902 54 33 519 1467 2579
Fragments arranged by size:
2579 1467 902 519 54 33
Bpu10I CC'TnA_GC
Cuts at: 2205 2205
Size: 5554
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3679 4544 4842 3679
Size: 865 298 4391
Fragments arranged by size:
4391 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4217 882
Size: 617 2718 2219
Fragments arranged by size:
2718 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3345 493
Size: 711 2141 2702
Fragments arranged by size:
2702 2141 711
BsaBI GATnn'nnATC
Cuts at: 5548 5548
Size: 5554
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3361 3743 4378 275
Size: 53 83 186 936 1828 382 635 1451
Fragments arranged by size:
1828 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2622 3987 4385 4659 4806 5217 2622
Size: 1365 398 274 147 411 2959
Fragments arranged by size:
2959 1365 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5329
Fragments arranged by size:
5329 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5554
BseYI C'CCAG_C
Cuts at: 2554 2815 4757 5253 2554
Size: 261 1942 496 2855
Fragments arranged by size:
2855 1942 496 261
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2237 2567 2675 1163
Size: 573 501 330 108 4042
Fragments arranged by size:
4042 573 501 330 108
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2715 3004 3765 4793 1540
Size: 275 186 714 289 761 1028 2301
Fragments arranged by size:
2301 1028 761 714 289 275 186
BsiHKAI G_wGCw'C
Cuts at: 2235 2735 2775 3615 3700 4771 2235
Size: 500 40 840 85 1071 3018
Fragments arranged by size:
3018 1071 840 500 85 40
BsmI GAATG_Cn'
Cuts at: 3065 3158 3065
Size: 93 5461
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2235 2410 2561 2735 2775 2873 3615
Size: 399 445 175 151 174 40 98 742
Cuts at: 3615 3700 4771 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 445 399 175 174 151
98 85 40
BspEI T'CCGG_A
Cuts at: 2622 5217 2622
Size: 2595 2959
BspHI T'CATG_A
Cuts at: 2301 3443 5173 2301
Size: 1142 1730 2682
Fragments arranged by size:
2682 1730 1142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2218 2909 844
Size: 1374 691 3489
Fragments arranged by size:
3489 1374 691
BsrBI CCG'CTC
Cuts at: 1394 1823 3441 1394
Size: 429 1618 3507
Fragments arranged by size:
3507 1618 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4049 4223 65
Size: 1183 2801 174 1396
Fragments arranged by size:
2801 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4198 4385 1700
Size: 110 2388 187 2869
Fragments arranged by size:
2869 2388 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5554
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5554
BssSI C'ACGA_G
Cuts at: 3614 4626 3614
Size: 1012 4542
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5554
BstBI TT'CG_AA
Cuts at: 4358 4358
Size: 5554
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5554
BstXI CCAn_nnnn'nTGG
Cuts at: 2173 2173
Size: 5554
BstYI r'GATC_y
Cuts at: 1972 2523 2884 3251 3636 3653 5105 5191 5203
Size: 551 361 367 385 17 1452 86 12
Cuts at: 5203 5549 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 551 385 367 361 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2660 513
Size: 552 939 656 3407
Fragments arranged by size:
3407 939 656 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4815
Fragments arranged by size:
4815 683 56
BtsI GCAGTG_nn'
Cuts at: 3070 3864 3884 3070
Size: 794 20 4740
Fragments arranged by size:
4740 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5554
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5519
DraI TTT'AAA
Cuts at: 2858 2858
Size: 5554
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4561 809
Size: 507 3245 1802
Fragments arranged by size:
3245 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2163 2478 2579
Size: 54 1425 50 275 18 333 315 101
Cuts at: 2579 2608 2712 3001 3890 5519 8
Size: 29 104 289 889 1629 43
Fragments arranged by size:
1629 1425 889 333 315 289 275 104
101 54 50 43 29 18
EagI C'GGCC_G
Cuts at: 1537 1812 2712 3001 1537
Size: 275 900 289 4090
Fragments arranged by size:
4090 900 289 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2508 3484 1353
Size: 1155 976 3423
Fragments arranged by size:
3423 1155 976
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2054 4141 4515 4661 387
Size: 1064 138 465 2087 374 146 1280
Fragments arranged by size:
2087 1280 1064 465 374 146 138
EcoICRI GAG'CTC
Cuts at: 2733 2773 2733
Size: 40 5514
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2141 2195 2228 2303 2666 2747
Size: 393 482 27 54 33 75 363 81
Cuts at: 2747 3617 4214 5001 5269 1239
Size: 870 597 787 268 1524
Fragments arranged by size:
1524 870 787 597 482 393 363 268
81 75 54 33 27
EcoO109I rG'GnC_Cy
Cuts at: 2158 2239 2278 2158
Size: 81 39 5434
Fragments arranged by size:
5434 81 39
EcoRI G'AATT_C
Cuts at: 2863 3241 2863
Size: 378 5176
FspI TGC'GCA
Cuts at: 4060 4060
Size: 5554
HaeII r_GCGC'y
Cuts at: 1678 2845 4701 1678
Size: 1167 1856 2531
Fragments arranged by size:
2531 1856 1167
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4195 4227 4269 4301 1373
Size: 32 135 32 2623 32 42 32 2626
Fragments arranged by size:
2626 2623 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2898 3144 669
Size: 988 281 960 246 3079
Fragments arranged by size:
3079 988 960 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5554
HpaI GTT'AAC
Cuts at: 3144 3144
Size: 5554
KpnI G_GTAC'C
Cuts at: 2404 2879 2404
Size: 475 5079
MfeI C'AATT_G
Cuts at: 3153 3153
Size: 5554
MluI A'CGCG_T
Cuts at: 5430 5430
Size: 5554
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2316 2649 4668 4852 1305
Size: 1011 333 2019 184 2007
Fragments arranged by size:
2019 2007 1011 333 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2165 10
Size: 54 1425 343 333 3399
Fragments arranged by size:
3399 1425 343 333 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2453 3512 3871 4030 518
Size: 648 1287 1059 359 159 2042
Fragments arranged by size:
2042 1287 1059 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2330 2551 2970 3647 4795 5040 1606
Size: 724 221 419 677 1148 245 2120
Fragments arranged by size:
2120 1148 724 677 419 245 221
NcoI C'CATG_G
Cuts at: 513 1065 2004 2660 513
Size: 552 939 656 3407
Fragments arranged by size:
3407 939 656 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5554
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5554
NotI GC'GGCC_GC
Cuts at: 3001 3001
Size: 5554
NspI r_CATG'y
Cuts at: 1132 1229 2458 2912 4457 1132
Size: 97 1229 454 1545 2229
Fragments arranged by size:
2229 1545 1229 454 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5554
PciI A'CATG_T
Cuts at: 2454 4453 2454
Size: 1999 3555
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5554
PfoI T'CCnGG_A
Cuts at: 2172 2172
Size: 5554
PmeI GTTT'AAAC
Cuts at: 2858 2858
Size: 5554
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3623 3655 5136 5168 3623
Size: 32 1481 32 4009
Fragments arranged by size:
4009 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2158 2278 2158
Size: 120 5434
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5554
PsiI TTA'TAA
Cuts at: 3124 3124
Size: 5554
PstI C_TGCA'G
Cuts at: 830 2229 2906 830
Size: 1399 677 3478
Fragments arranged by size:
3478 1399 677
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5554
PvuII CAG'CTG
Cuts at: 2551 2551
Size: 5554
SacI G_AGCT'C
Cuts at: 2735 2775 2735
Size: 40 5514
SalI G'TCGA_C
Cuts at: 1936 2896 1936
Size: 960 4594
SbfI CC_TGCA'GG
Cuts at: 2906 2906
Size: 5554
ScaI AGT'ACT
Cuts at: 3802 5514 3802
Size: 1712 3842
SfcI C'TryA_G
Cuts at: 826 1045 2225 2902 4037 4718 4909 826
Size: 219 1180 677 1135 681 191 1471
Fragments arranged by size:
1471 1180 1135 681 677 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5554
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5554
SmaI CCC'GGG
Cuts at: 1872 2348 2881 1872
Size: 476 533 4545
Fragments arranged by size:
4545 533 476
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3658 4559 4821 820
Size: 197 926 1715 901 262 1553
Fragments arranged by size:
1715 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3345 493
Size: 2852 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5554
SphI G_CATG'C
Cuts at: 1132 2912 1132
Size: 1780 3774
SrfI GCCC'GGGC
Cuts at: 2348 2348
Size: 5554
SspI AAT'ATT
Cuts at: 5 52 3478 5
Size: 47 3426 2081
Fragments arranged by size:
3426 2081 47
StuI AGG'CCT
Cuts at: 1517 2500 1517
Size: 983 4571
StyI C'CwwG_G
Cuts at: 513 1065 2004 2382 2660 2993 513
Size: 552 939 378 278 333 3074
Fragments arranged by size:
3074 939 552 378 333 278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2321 3581 3598 3751 3936 4381 325
Size: 1255 741 1260 17 153 185 445 1498
Fragments arranged by size:
1498 1260 1255 741 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2613 3800 5512
Size: 274 80 33 51 1443 636 1187 1712
Cuts at: 5512 96
Size: 138
Fragments arranged by size:
1712 1443 1187 636 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5514 5546 5514
Size: 32 5522
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5554
XbaI T'CTAG_A
Cuts at: 2890 2890
Size: 5554
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2599 2599
Size: 5554
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5554
XmaI C'CCGG_G
Cuts at: 1870 2346 2879 1870
Size: 476 533 4545
Fragments arranged by size:
4545 533 476
XmnI GAAnn'nnTTC
Cuts at: 3683 3683
Size: 5554
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3362 276
Size: 53 83 186 936 1828 2468
Fragments arranged by size:
2468 1828 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SwaI