Restriction map for
pFN21AB8429
(Circular) (Six-base) MAPSORT of: pFN21AB8429.seq Check: 1902 from: 1 to: 6010
pFN21AB8429 6010 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3031 3031
Size: 6010
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2976 3820 278
Size: 53 83 186 936 1440 844 2468
Fragments arranged by size:
2468 1440 936 844 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2876 3353 1937
Size: 939 477 4594
Fragments arranged by size:
4594 939 477
Acc65I G'GTAC_C
Cuts at: 3199 3331 3199
Size: 132 5878
AclI AA'CG_TT
Cuts at: 1563 4137 4510 1563
Size: 2574 373 3063
Fragments arranged by size:
3063 2574 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2219 2434 2618 2714 4073 5457 5725 1632
Size: 587 215 184 96 1359 1384 268 1917
Fragments arranged by size:
1917 1384 1359 587 268 215 184 96
AfeI AGC'GCT
Cuts at: 3296 3296
Size: 6010
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5813
AflIII A'CryG_T
Cuts at: 1203 2772 4909 5886 1203
Size: 1569 2137 977 1327
Fragments arranged by size:
2137 1569 1327 977
AgeI A'CCGG_T
Cuts at: 2137 3229 4841 2137
Size: 1092 1612 3306
Fragments arranged by size:
3306 1612 1092
AhdI GACnn_n'nnGTC
Cuts at: 4739 4739
Size: 6010
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6010
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2285 1184
Size: 1101 4909
AlwNI CAG_nnn'CTG
Cuts at: 2959 3016 3151 3154 3753 5325 2959
Size: 57 135 3 599 1572 3644
Fragments arranged by size:
3644 1572 599 135 57 3
ApaI G_GGCC'C
Cuts at: 2782 2782
Size: 6010
ApaLI G'TGCA_C
Cuts at: 2324 2585 4067 5223 2324
Size: 261 1482 1156 3111
Fragments arranged by size:
3111 1482 1156 261
ApoI r'AATT_y
Cuts at: 1493 3319 3545 3697 5806 1493
Size: 1826 226 152 2109 1697
Fragments arranged by size:
2109 1826 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4564 160
Size: 872 3532 1606
Fragments arranged by size:
3532 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
AvaI C'yCGr_G
Cuts at: 1870 1943 2660 3335 1870
Size: 73 717 675 4545
Fragments arranged by size:
4545 717 675 73
BamHI G'GATC_C
Cuts at: 3340 3707 3340
Size: 367 5643
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2140 3199 3331 4786 618
Size: 325 217 627 353 1059 132 1455 1842
Fragments arranged by size:
1842 1455 1059 627 353 325 217 132
BanII G_rGCy'C
Cuts at: 1391 2714 2782 2850 3176 3329 1391
Size: 1323 68 68 326 153 4072
Fragments arranged by size:
4072 1323 326 153 68 68
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5039
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2401 2435 4201 4235 1903
Size: 34 464 34 1766 34 3678
Fragments arranged by size:
3678 1766 464 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3902 5112 3902
Size: 1210 4800
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6010
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3287 4621 136
Size: 107 122 71 631 605 1615 1334 1525
Fragments arranged by size:
1615 1525 1334 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6005 6005
Size: 6010
BlpI GC'TnA_GC
Cuts at: 2510 2738 3427 2510
Size: 228 689 5093
Fragments arranged by size:
5093 689 228
Bme1580I G_kGCm'C
Cuts at: 1790 2328 2589 2782 4071 5227 1790
Size: 538 261 193 1289 1156 2573
Fragments arranged by size:
2573 1289 1156 538 261 193
BmgBI CAC'GTC
Cuts at: 1318 2545 2734 1318
Size: 1227 189 4594
Fragments arranged by size:
4594 1227 189
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2760 3112 3741
Size: 416 53 83 399 24 1343 352 629
Cuts at: 3741 4699 442
Size: 958 1753
Fragments arranged by size:
1753 1343 958 629 416 399 352 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6010
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3130 3190 3130
Size: 60 5950
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2234 2999 4670 1239
Size: 995 765 1671 2579
Fragments arranged by size:
2579 1671 995 765
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4135 5000 5298 4135
Size: 865 298 4847
Fragments arranged by size:
4847 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2586 4673 882
Size: 617 1087 2087 2219
Fragments arranged by size:
2219 2087 1087 617
BsaAI yAC'GTr
Cuts at: 493 1204 3801 493
Size: 711 2597 2702
Fragments arranged by size:
2702 2597 711
BsaBI GATnn'nnATC
Cuts at: 2642 6004 2642
Size: 3362 2648
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2973 3186 3817 4199
Size: 53 83 186 936 1440 213 631 382
Cuts at: 4199 4834 275
Size: 635 1451
Fragments arranged by size:
1451 1440 936 635 631 382 213 186
83 53
BsaWI w'CCGG_w
Cuts at: 2137 2925 3229 4443 4841 5115 5262 5673 2137
Size: 788 304 1214 398 274 147 411 2474
Fragments arranged by size:
2474 1214 788 411 398 304 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5785
Fragments arranged by size:
5785 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2043 2231 3122 3179 1186
Size: 857 188 891 57 4017
Fragments arranged by size:
4017 891 857 188 57
BseYI C'CCAG_C
Cuts at: 2033 2602 2949 3195 3241 5213 5709 2033
Size: 569 347 246 46 1972 496 2334
Fragments arranged by size:
2334 1972 569 496 347 246 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2174 2258 2288 2552 1163
Size: 573 438 84 30 264 4621
Fragments arranged by size:
4621 573 438 264 84 30
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2746 2750 3460 4221 5249 1540
Size: 275 186 745 4 710 761 1028 2301
Fragments arranged by size:
2301 1028 761 745 710 275 186 4
BsiHKAI G_wGCw'C
Cuts at: 2328 2443 2589 2619 2739 2796 2850 3116 3176
Size: 115 146 30 120 57 54 266 60
Cuts at: 3176 4071 4156 5227 2328
Size: 895 85 1071 3111
Fragments arranged by size:
3111 1071 895 266 146 120 115 85
60 57 54 30
BsmI GAATG_Cn'
Cuts at: 3521 3614 3521
Size: 93 5917
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2328 2443 2589 2619 2714 2739 2782
Size: 399 538 115 146 30 95 25 43
Cuts at: 2782 2796 2850 3116 3176 3329 4071 4156 5227
Size: 14 54 266 60 153 742 85 1071
Cuts at: 5227 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 538 399 266 153 146
115 95 85 60 54 43 30 25
14
BspEI T'CCGG_A
Cuts at: 2925 5673 2925
Size: 2748 3262
BspHI T'CATG_A
Cuts at: 2443 3899 5629 2443
Size: 1456 1730 2824
Fragments arranged by size:
2824 1730 1456
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2487 2697 3031 3309 3365 844
Size: 1643 210 334 278 56 3489
Fragments arranged by size:
3489 1643 334 278 210 56
BsrBI CCG'CTC
Cuts at: 1394 1823 2254 3897 1394
Size: 429 431 1643 3507
Fragments arranged by size:
3507 1643 431 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2997 3079 4505 4679 65
Size: 1183 1749 82 1426 174 1396
Fragments arranged by size:
1749 1426 1396 1183 174 82
BsrFI r'CCGG_y
Cuts at: 1700 1810 2137 2745 3229 4654 4841 1700
Size: 110 327 608 484 1425 187 2869
Fragments arranged by size:
2869 1425 608 484 327 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6010
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6010
BssSI C'ACGA_G
Cuts at: 4070 5082 4070
Size: 1012 4998
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3154 794
Size: 2360 3650
BstBI TT'CG_AA
Cuts at: 4814 4814
Size: 6010
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6010
BstXI CCAn_nnnn'nTGG
Cuts at: 2062 2389 2062
Size: 327 5683
BstYI r'GATC_y
Cuts at: 1972 2202 3340 3707 4092 4109 5561 5647 5659
Size: 230 1138 367 385 17 1452 86 12
Cuts at: 5659 6005 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1138 385 367 346 230 86
17 12
BstZ17I GTA'TAC
Cuts at: 2877 2877
Size: 6010
Bsu36I CC'TnA_GG
Cuts at: 2886 2886
Size: 6010
BtgI C'CryG_G
Cuts at: 513 1065 2332 2755 513
Size: 552 1267 423 3768
Fragments arranged by size:
3768 1267 552 423
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5271
Fragments arranged by size:
5271 683 56
BtsI GCAGTG_nn'
Cuts at: 2162 2288 3526 4320 4340 2162
Size: 126 1238 794 20 3832
Fragments arranged by size:
3832 1238 794 126 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6010
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5975
DraI TTT'AAA
Cuts at: 3314 3314
Size: 6010
DraIII CAC_nnn'GTG
Cuts at: 2916 2916
Size: 6010
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5017 809
Size: 507 3701 1802
Fragments arranged by size:
3701 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2743 2747
Size: 54 1425 50 275 18 216 697 4
Cuts at: 2747 2812 2961 3159 3279 3457 4346 5975 8
Size: 65 149 198 120 178 889 1629 43
Fragments arranged by size:
1629 1425 889 697 275 216 198 178
149 120 65 54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2743 2747 3457 1537
Size: 275 931 4 710 4090
Fragments arranged by size:
4090 931 710 275 4
EarI CTCTTCn'nnn_
Cuts at: 1353 2244 2345 2498 2801 2855 3164 3940 1353
Size: 891 101 153 303 54 309 776 3423
Fragments arranged by size:
3423 891 776 309 303 153 101 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4597 4971 5117 387
Size: 1064 138 3008 374 146 1280
Fragments arranged by size:
3008 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2848 3174 2848
Size: 326 5684
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2219 2234 2434 2618 2714 2999 4073
Size: 393 587 15 200 184 96 285 1074
Cuts at: 4073 4670 5457 5725 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1074 787 597 587 393 285 268
200 184 96 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2342 2693 2342
Size: 351 5659
EcoO109I rG'GnC_Cy
Cuts at: 2563 2686 2779 2983 2563
Size: 123 93 204 5590
Fragments arranged by size:
5590 204 123 93
EcoRI G'AATT_C
Cuts at: 3319 3697 3319
Size: 378 5632
FseI GG_CCGG'CC
Cuts at: 2749 2749
Size: 6010
FspI TGC'GCA
Cuts at: 4516 4516
Size: 6010
HaeII r_GCGC'y
Cuts at: 1678 2959 3298 5157 1678
Size: 1281 339 1859 2531
Fragments arranged by size:
2531 1859 1281 339
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2094 2126 2363 2395 2617
Size: 32 135 32 522 32 237 32 222
Cuts at: 2617 2649 4651 4683 4725 4757 1373
Size: 32 2002 32 42 32 2626
Fragments arranged by size:
2626 2002 522 237 222 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3354 3600 669
Size: 988 281 1416 246 3079
Fragments arranged by size:
3079 1416 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6010
HpaI GTT'AAC
Cuts at: 3600 3600
Size: 6010
KpnI G_GTAC'C
Cuts at: 3203 3335 3203
Size: 132 5878
MfeI C'AATT_G
Cuts at: 3609 3609
Size: 6010
MluI A'CGCG_T
Cuts at: 5886 5886
Size: 6010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5124 5308 1305
Size: 3819 184 2007
Fragments arranged by size:
3819 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2814 10
Size: 54 1425 343 982 3206
Fragments arranged by size:
3206 1425 982 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3968 4327 4486 518
Size: 648 2802 359 159 2042
Fragments arranged by size:
2802 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2197 2465 2742 3151 3426 4103 5251 5496
Size: 591 268 277 409 275 677 1148 245
Cuts at: 5496 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 591 409 277 275 268
245
NaeI GCC'GGC
Cuts at: 2747 2747
Size: 6010
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5458
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6010
NgoMIV G'CCGG_C
Cuts at: 2745 2745
Size: 6010
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6010
NotI GC'GGCC_GC
Cuts at: 3457 3457
Size: 6010
NspI r_CATG'y
Cuts at: 1132 1229 2776 3142 3368 4913 1132
Size: 97 1547 366 226 1545 2229
Fragments arranged by size:
2229 1547 1545 366 226 97
PasI CC'CwG_GG
Cuts at: 1348 2782 1348
Size: 1434 4576
PciI A'CATG_T
Cuts at: 2772 4909 2772
Size: 2137 3873
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6010
PfoI T'CCnGG_A
Cuts at: 2343 2424 2976 2343
Size: 81 552 5377
Fragments arranged by size:
5377 552 81
PmeI GTTT'AAAC
Cuts at: 3314 3314
Size: 6010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4079 4111 5592 5624 4079
Size: 32 1481 32 4465
Fragments arranged by size:
4465 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2686 2686
Size: 6010
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6010
PsiI TTA'TAA
Cuts at: 3580 3580
Size: 6010
PspOMI G'GGCC_C
Cuts at: 2778 2778
Size: 6010
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2801 2833 2801
Size: 32 5978
PstI C_TGCA'G
Cuts at: 830 2484 3362 830
Size: 1654 878 3478
Fragments arranged by size:
3478 1654 878
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6010
PvuII CAG'CTG
Cuts at: 2197 3151 2197
Size: 954 5056
SacI G_AGCT'C
Cuts at: 2850 3176 2850
Size: 326 5684
SalI G'TCGA_C
Cuts at: 1936 3352 1936
Size: 1416 4594
SapI GCTCTTCn'nnn_
Cuts at: 2244 2801 2855 3164 2244
Size: 557 54 309 5090
Fragments arranged by size:
5090 557 309 54
SbfI CC_TGCA'GG
Cuts at: 3362 3362
Size: 6010
ScaI AGT'ACT
Cuts at: 2285 4258 5970 2285
Size: 1973 1712 2325
Fragments arranged by size:
2325 1973 1712
SfcI C'TryA_G
Cuts at: 826 1045 2480 3358 4493 5174 5365 826
Size: 219 1435 878 1135 681 191 1471
Fragments arranged by size:
1471 1435 1135 878 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
SgrAI Cr'CCGG_yG
Cuts at: 1700 2137 3229 1700
Size: 437 1092 4481
Fragments arranged by size:
4481 1092 437
SmaI CCC'GGG
Cuts at: 1872 3337 1872
Size: 1465 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4114 5015 5277 820
Size: 197 926 2171 901 262 1553
Fragments arranged by size:
2171 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3801 493
Size: 3308 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6010
SphI G_CATG'C
Cuts at: 1132 3368 1132
Size: 2236 3774
SspI AAT'ATT
Cuts at: 5 52 3934 5
Size: 47 3882 2081
Fragments arranged by size:
3882 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6010
StyI C'CwwG_G
Cuts at: 513 1065 2055 2113 2230 3270 3449 513
Size: 552 990 58 117 1040 179 3074
Fragments arranged by size:
3074 1040 990 552 179 117 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4037 4054 4207 4392 4837 325
Size: 1255 2457 17 153 185 445 1498
Fragments arranged by size:
2457 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2283 4256 5968
Size: 274 80 33 51 1443 306 1973 1712
Cuts at: 5968 96
Size: 138
Fragments arranged by size:
1973 1712 1443 306 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5970 6002 5970
Size: 32 5978
Tth111I GACn'n_nGTC
Cuts at: 1316 2546 1316
Size: 1230 4780
XbaI T'CTAG_A
Cuts at: 3346 3346
Size: 6010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3268 3268
Size: 6010
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6010
XmaI C'CCGG_G
Cuts at: 1870 3335 1870
Size: 1465 4545
XmnI GAAnn'nnTTC
Cuts at: 4139 4139
Size: 6010
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2974 3818 276
Size: 53 83 186 936 1440 844 2468
Fragments arranged by size:
2468 1440 936 844 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI EcoRV FalI FspAI KasI NarI NruI
NsiI PacI PmlI PspXI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI