Restriction map for
pFN21AB8428
(Circular) (Six-base) MAPSORT of: pFN21AB8428.seq Check: 1978 from: 1 to: 6106
pFN21AB8428 6106 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2713 2948 2713
Size: 235 5871
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3916 278
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3449 1937
Size: 1512 4594
Acc65I G'GTAC_C
Cuts at: 3427 3427
Size: 6106
AclI AA'CG_TT
Cuts at: 1563 4233 4606 1563
Size: 2670 373 3063
Fragments arranged by size:
3063 2670 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2597 4169 5553 5821 1632
Size: 965 1572 1384 268 1917
Fragments arranged by size:
1917 1572 1384 965 268
AflII C'TTAA_G
Cuts at: 820 1017 2642 820
Size: 197 1625 4284
Fragments arranged by size:
4284 1625 197
AflIII A'CryG_T
Cuts at: 1203 3090 5005 5982 1203
Size: 1887 1915 977 1327
Fragments arranged by size:
1915 1887 1327 977
AgeI A'CCGG_T
Cuts at: 2532 4937 2532
Size: 2405 3701
AhdI GACnn_n'nnGTC
Cuts at: 2950 4835 2950
Size: 1885 4221
AleI CACnn'nnGTG
Cuts at: 1166 2598 1166
Size: 1432 4674
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3162 1184
Size: 1978 4128
AlwNI CAG_nnn'CTG
Cuts at: 2069 2474 2755 2806 2815 3037 3175 3275 3849
Size: 405 281 51 9 222 138 100 574
Cuts at: 3849 5421 2069
Size: 1572 2754
Fragments arranged by size:
2754 1572 574 405 281 222 138 100
51 9
ApaI G_GGCC'C
Cuts at: 3224 3224
Size: 6106
ApaLI G'TGCA_C
Cuts at: 3086 4163 5319 3086
Size: 1077 1156 3873
Fragments arranged by size:
3873 1156 1077
ApoI r'AATT_y
Cuts at: 1493 3415 3641 3793 5902 1493
Size: 1922 226 152 2109 1697
Fragments arranged by size:
2109 1922 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4660 160
Size: 872 3628 1606
Fragments arranged by size:
3628 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
AvaI C'yCGr_G
Cuts at: 1870 1943 2042 2153 3201 3431 1870
Size: 73 99 111 1048 230 4545
Fragments arranged by size:
4545 1048 230 111 99 73
BamHI G'GATC_C
Cuts at: 3436 3803 3436
Size: 367 5739
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3180 3427 4882 618
Size: 325 217 627 1393 247 1455 1842
Fragments arranged by size:
1842 1455 1393 627 325 247 217
BanII G_rGCy'C
Cuts at: 1391 2270 3065 3135 3224 3425 1391
Size: 879 795 70 89 201 4072
Fragments arranged by size:
4072 879 795 201 89 70
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2577 928
Size: 971 678 4457
Fragments arranged by size:
4457 971 678
BbvCI CC'TCA_GC
Cuts at: 2492 2492
Size: 6106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4297 4331 1903
Size: 34 2360 34 3678
Fragments arranged by size:
3678 2360 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2234 3998 5208 2234
Size: 1764 1210 3132
Fragments arranged by size:
3132 1764 1210
BclI T'GATC_A
Cuts at: 1548 2817 1548
Size: 1269 4837
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4717 136
Size: 107 122 71 631 605 3045 1525
Fragments arranged by size:
3045 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6101 6101
Size: 6106
BlpI GC'TnA_GC
Cuts at: 2604 3059 3261 3523 2604
Size: 455 202 262 5187
Fragments arranged by size:
5187 455 262 202
Bme1580I G_kGCm'C
Cuts at: 1790 2670 3090 3224 4167 5323 1790
Size: 880 420 134 943 1156 2573
Fragments arranged by size:
2573 1156 943 880 420 134
BmgBI CAC'GTC
Cuts at: 1318 2351 1318
Size: 1033 5073
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2217 3837 4795
Size: 416 53 83 399 24 800 1620 958
Cuts at: 4795 442
Size: 1753
Fragments arranged by size:
1753 1620 958 800 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6106
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3385 3385
Size: 6106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3062 3355 4766 1239
Size: 1823 293 1411 2579
Fragments arranged by size:
2579 1823 1411 293
Bpu10I CC'TnA_GC
Cuts at: 2492 2492
Size: 6106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2714 3119 4231 5096 5394 2714
Size: 405 1112 865 298 3426
Fragments arranged by size:
3426 1112 865 405 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4769 882
Size: 617 3270 2219
Fragments arranged by size:
3270 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3897 493
Size: 711 2693 2702
Fragments arranged by size:
2702 2693 711
BsaBI GATnn'nnATC
Cuts at: 6100 6100
Size: 6106
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3913 4295 4930 275
Size: 53 83 186 936 2380 382 635 1451
Fragments arranged by size:
2380 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2532 2709 4539 4937 5211 5358 5769 2532
Size: 177 1830 398 274 147 411 2869
Fragments arranged by size:
2869 1830 411 398 274 177 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2399 2414 2429 2444 1178
Size: 30 165 30 996 15 15 15 4840
Fragments arranged by size:
4840 996 165 30 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2375 2747 2750 3092 1186
Size: 1189 372 3 342 4200
Fragments arranged by size:
4200 1189 372 342 3
BseYI C'CCAG_C
Cuts at: 2663 5309 5805 2663
Size: 2646 496 2964
Fragments arranged by size:
2964 2646 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2373 2423 1163
Size: 573 637 50 4846
Fragments arranged by size:
4846 637 573 50
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3556 4317 5345 1540
Size: 275 186 1555 761 1028 2301
Fragments arranged by size:
2301 1555 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3090 3135 4167 4252 5323 3090
Size: 45 1032 85 1071 3873
Fragments arranged by size:
3873 1071 1032 85 45
BsmI GAATG_Cn'
Cuts at: 3617 3710 3617
Size: 93 6013
BsmBI CGTCTCn'nnnn_
Cuts at: 2705 2705
Size: 6106
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2270 2670 3065 3090 3135 3224 3425
Size: 399 480 400 395 25 45 89 201
Cuts at: 3425 4167 4252 5323 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 480 400 399 395 201
89 85 45 25
BspEI T'CCGG_A
Cuts at: 5769 5769
Size: 6106
BspHI T'CATG_A
Cuts at: 3187 3995 5725 3187
Size: 808 1730 3568
Fragments arranged by size:
3568 1730 808
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2517 2713 2751 2814 2948 3461 844
Size: 1673 196 38 63 134 513 3489
Fragments arranged by size:
3489 1673 513 196 134 63 38
BsrBI CCG'CTC
Cuts at: 1394 1823 2584 2785 3993 1394
Size: 429 761 201 1208 3507
Fragments arranged by size:
3507 1208 761 429 201
BsrDI GCAATG_nn'
Cuts at: 65 1248 2337 4601 4775 65
Size: 1183 1089 2264 174 1396
Fragments arranged by size:
2264 1396 1183 1089 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2532 2908 3265 3403 4750 4937 1700
Size: 110 722 376 357 138 1347 187 2869
Fragments arranged by size:
2869 1347 722 376 357 187 138 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6106
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6106
BssSI C'ACGA_G
Cuts at: 4166 5178 4166
Size: 1012 5094
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3175 794
Size: 2381 3725
BstBI TT'CG_AA
Cuts at: 4910 4910
Size: 6106
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6106
BstYI r'GATC_y
Cuts at: 1972 3436 3803 4188 4205 5657 5743 5755 6101
Size: 1464 367 385 17 1452 86 12 346
Cuts at: 6101 1972
Size: 1977
Fragments arranged by size:
1977 1464 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4615
Fragments arranged by size:
4615 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5367
Fragments arranged by size:
5367 683 56
BtsI GCAGTG_nn'
Cuts at: 2213 2835 3276 3622 4416 4436 2213
Size: 622 441 346 794 20 3883
Fragments arranged by size:
3883 794 622 441 346 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6106
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2982 3017 529
Size: 35 2418 35 3618
Fragments arranged by size:
3618 2418 35 35
DraI TTT'AAA
Cuts at: 3410 3410
Size: 6106
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5113 809
Size: 507 3797 1802
Fragments arranged by size:
3797 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2233 3553 4442
Size: 54 1425 50 275 18 403 1320 889
Cuts at: 4442 6071 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1320 889 403 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3553 1537
Size: 275 1741 4090
Fragments arranged by size:
4090 1741 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4036 1353
Size: 2683 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2695 4693 5067 5213 387
Size: 1064 138 1106 1998 374 146 1280
Fragments arranged by size:
1998 1280 1106 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3133 3133
Size: 6106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2597 3062 3355 4169 4766 5553 5821
Size: 393 965 465 293 814 597 787 268
Cuts at: 5821 1239
Size: 1524
Fragments arranged by size:
1524 965 814 787 597 465 393 293
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2383 2747 2383
Size: 364 5742
EcoO109I rG'GnC_Cy
Cuts at: 2803 2866 3022 3220 3221 2803
Size: 63 156 198 1 5688
Fragments arranged by size:
5688 198 156 63 1
EcoRI G'AATT_C
Cuts at: 3415 3793 3415
Size: 378 5728
FspI TGC'GCA
Cuts at: 2759 4612 2759
Size: 1853 4253
HaeII r_GCGC'y
Cuts at: 1678 5253 1678
Size: 3575 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2051 2083 2936 2968 4747
Size: 32 135 32 479 32 853 32 1779
Cuts at: 4747 4779 4821 4853 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1779 853 479 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3450 3696 669
Size: 988 281 1512 246 3079
Fragments arranged by size:
3079 1512 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2606 748
Size: 1858 4248
HpaI GTT'AAC
Cuts at: 3696 3696
Size: 6106
KpnI G_GTAC'C
Cuts at: 3431 3431
Size: 6106
MfeI C'AATT_G
Cuts at: 3705 3705
Size: 6106
MluI A'CGCG_T
Cuts at: 5982 5982
Size: 6106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5220 5404 1305
Size: 3915 184 2007
Fragments arranged by size:
3915 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2235 10
Size: 54 1425 343 403 3881
Fragments arranged by size:
3881 1425 403 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2451 2598 3186 3317 4064 4423 4582
Size: 648 1285 147 588 131 747 359 159
Cuts at: 4582 518
Size: 2042
Fragments arranged by size:
2042 1285 747 648 588 359 159 147
131
MspA1I CmG'CkG
Cuts at: 1606 2357 2825 2848 3522 4199 5347 5592 1606
Size: 751 468 23 674 677 1148 245 2120
Fragments arranged by size:
2120 1148 751 677 674 468 245 23
NaeI GCC'GGC
Cuts at: 2910 3267 2910
Size: 357 5749
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4615
Fragments arranged by size:
4615 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6106
NgoMIV G'CCGG_C
Cuts at: 2908 3265 2908
Size: 357 5749
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6106
NotI GC'GGCC_GC
Cuts at: 3553 3553
Size: 6106
NspI r_CATG'y
Cuts at: 1132 1229 2164 3094 3464 5009 1132
Size: 97 935 930 370 1545 2229
Fragments arranged by size:
2229 1545 935 930 370 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6106
PciI A'CATG_T
Cuts at: 3090 5005 3090
Size: 1915 4191
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6106
PmeI GTTT'AAAC
Cuts at: 3410 3410
Size: 6106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4175 4207 5688 5720 4175
Size: 32 1481 32 4561
Fragments arranged by size:
4561 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2803 2803
Size: 6106
PshAI GACnn'nnGTC
Cuts at: 1613 3361 1613
Size: 1748 4358
PsiI TTA'TAA
Cuts at: 3676 3676
Size: 6106
PspOMI G'GGCC_C
Cuts at: 3220 3220
Size: 6106
PstI C_TGCA'G
Cuts at: 830 2811 2842 2929 2959 3171 3458 830
Size: 1981 31 87 30 212 287 3478
Fragments arranged by size:
3478 1981 287 212 87 31 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6106
SacI G_AGCT'C
Cuts at: 3135 3135
Size: 6106
SalI G'TCGA_C
Cuts at: 1936 3448 1936
Size: 1512 4594
SbfI CC_TGCA'GG
Cuts at: 3458 3458
Size: 6106
ScaI AGT'ACT
Cuts at: 4354 6066 4354
Size: 1712 4394
SexAI A'CCwGG_T
Cuts at: 3114 3114
Size: 6106
SfcI C'TryA_G
Cuts at: 826 1045 2747 2807 2838 2925 2955 3167 3454
Size: 219 1702 60 31 87 30 212 287
Cuts at: 3454 4589 5270 5461 826
Size: 1135 681 191 1471
Fragments arranged by size:
1702 1471 1135 681 287 219 212 191
87 60 31 30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6106
SmaI CCC'GGG
Cuts at: 1872 3203 3433 1872
Size: 1331 230 4545
Fragments arranged by size:
4545 1331 230
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2642 2693 3134 4210 5111 5373
Size: 197 926 699 51 441 1076 901 262
Cuts at: 5373 820
Size: 1553
Fragments arranged by size:
1553 1076 926 901 699 441 262 197
51
SnaBI TAC'GTA
Cuts at: 493 3897 493
Size: 3404 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6106
SphI G_CATG'C
Cuts at: 1132 2164 3464 1132
Size: 1032 1300 3774
Fragments arranged by size:
3774 1300 1032
SrfI GCCC'GGGC
Cuts at: 3203 3203
Size: 6106
SspI AAT'ATT
Cuts at: 5 52 4030 5
Size: 47 3978 2081
Fragments arranged by size:
3978 2081 47
StuI AGG'CCT
Cuts at: 1517 3143 1517
Size: 1626 4480
StyI C'CwwG_G
Cuts at: 513 1065 2004 2285 3300 3545 513
Size: 552 939 281 1015 245 3074
Fragments arranged by size:
3074 1015 939 552 281 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4133 4150 4303 4488 4933 325
Size: 1255 2553 17 153 185 445 1498
Fragments arranged by size:
2553 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2415 4352 6064
Size: 274 80 33 51 1443 438 1937 1712
Cuts at: 6064 96
Size: 138
Fragments arranged by size:
1937 1712 1443 438 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6066 6098 6066
Size: 32 6074
Tth111I GACn'n_nGTC
Cuts at: 1316 3288 1316
Size: 1972 4134
XbaI T'CTAG_A
Cuts at: 2035 3442 2035
Size: 1407 4699
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3316 3316
Size: 6106
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6106
XmaI C'CCGG_G
Cuts at: 1870 3201 3431 1870
Size: 1331 230 4545
Fragments arranged by size:
4545 1331 230
XmnI GAAnn'nnTTC
Cuts at: 4235 4235
Size: 6106
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3914 276
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspXI
PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI
SwaI