Restriction map for
pFN21AB8422
(Circular) (Six-base) MAPSORT of: pFN21AB8422.seq Check: 5951 from: 1 to: 6937
pFN21AB8422 6937 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2783 2898 3588 2783
Size: 115 690 6132
Fragments arranged by size:
6132 690 115
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4747 278
Size: 53 83 186 936 3211 2468
Fragments arranged by size:
3211 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4280 1937
Size: 2343 4594
Acc65I G'GTAC_C
Cuts at: 4258 4258
Size: 6937
AclI AA'CG_TT
Cuts at: 1563 5064 5437 1563
Size: 3501 373 3063
Fragments arranged by size:
3501 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2581 2996 3164 3656 5000 6384 6652 1632
Size: 949 415 168 492 1344 1384 268 1917
Fragments arranged by size:
1917 1384 1344 949 492 415 268 168
AfeI AGC'GCT
Cuts at: 2759 2759
Size: 6937
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6740
AflIII A'CryG_T
Cuts at: 1203 2847 3403 5836 6813 1203
Size: 1644 556 2433 977 1327
Fragments arranged by size:
2433 1644 1327 977 556
AgeI A'CCGG_T
Cuts at: 5768 5768
Size: 6937
AhdI GACnn_n'nnGTC
Cuts at: 5666 5666
Size: 6937
AleI CACnn'nnGTG
Cuts at: 1166 3084 3336 1166
Size: 1918 252 4767
Fragments arranged by size:
4767 1918 252
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6937
AlwNI CAG_nnn'CTG
Cuts at: 3849 4680 6252 3849
Size: 831 1572 4534
Fragments arranged by size:
4534 1572 831
ApaI G_GGCC'C
Cuts at: 3173 4139 3173
Size: 966 5971
ApaLI G'TGCA_C
Cuts at: 2851 4994 6150 2851
Size: 2143 1156 3638
Fragments arranged by size:
3638 2143 1156
ApoI r'AATT_y
Cuts at: 1493 3542 3626 4246 4472 4624 6733 1493
Size: 2049 84 620 226 152 2109 1697
Fragments arranged by size:
2109 2049 1697 620 226 152 84
AseI AT'TA_AT
Cuts at: 160 1032 5491 160
Size: 872 4459 1606
Fragments arranged by size:
4459 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6937
AvaI C'yCGr_G
Cuts at: 1870 1943 2106 2207 2548 3217 3938 3971 4262
Size: 73 163 101 341 669 721 33 291
Cuts at: 4262 1870
Size: 4545
Fragments arranged by size:
4545 721 669 341 291 163 101 73
33
BamHI G'GATC_C
Cuts at: 4267 4634 4267
Size: 367 6570
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3805 4258 5713 618
Size: 325 217 627 2018 453 1455 1842
Fragments arranged by size:
2018 1842 1455 627 453 325 217
BanII G_rGCy'C
Cuts at: 1391 2661 3060 3173 3774 3894 4139 4256 1391
Size: 1270 399 113 601 120 245 117 4072
Fragments arranged by size:
4072 1270 601 399 245 120 117 113
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2072 2206 928
Size: 971 173 134 5659
Fragments arranged by size:
5659 971 173 134
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3208 3242 5128 5162 1903
Size: 34 50 34 1187 34 1886 34 3678
Fragments arranged by size:
3678 1886 1187 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4829 6039 4829
Size: 1210 5727
BclI T'GATC_A
Cuts at: 1548 2025 1548
Size: 477 6460
BfrBI ATG'CAT
Cuts at: 4013 4013
Size: 6937
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5548 136
Size: 107 122 71 631 605 3876 1525
Fragments arranged by size:
3876 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6932 6932
Size: 6937
BlpI GC'TnA_GC
Cuts at: 2882 4354 2882
Size: 1472 5465
Bme1580I G_kGCm'C
Cuts at: 1790 2577 2771 2855 2921 3173 3838 4139 4998
Size: 787 194 84 66 252 665 301 859
Cuts at: 4998 6154 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 859 787 665 301 252 194
84 66
BmgBI CAC'GTC
Cuts at: 1318 2704 2740 3508 1318
Size: 1386 36 768 4747
Fragments arranged by size:
4747 1386 768 36
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2158 4668 5626
Size: 416 53 83 399 24 741 2510 958
Cuts at: 5626 442
Size: 1753
Fragments arranged by size:
2510 1753 958 741 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6937
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3779 4001 4217 5597 1239
Size: 2540 222 216 1380 2579
Fragments arranged by size:
2579 2540 1380 222 216
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3044 3296 3464 5062 5927 6225 3044
Size: 252 168 1598 865 298 3756
Fragments arranged by size:
3756 1598 865 298 252 168
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2184 2669 5600 882
Size: 617 685 485 2931 2219
Fragments arranged by size:
2931 2219 685 617 485
BsaAI yAC'GTr
Cuts at: 493 1204 2734 3406 4728 493
Size: 711 1530 672 1322 2702
Fragments arranged by size:
2702 1530 1322 711 672
BsaBI GATnn'nnATC
Cuts at: 6931 6931
Size: 6937
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4744 5126 5761 275
Size: 53 83 186 936 3211 382 635 1451
Fragments arranged by size:
3211 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3549 5370 5768 6042 6189 6600 3549
Size: 1821 398 274 147 411 3886
Fragments arranged by size:
3886 1821 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2488 2518 3173 3203 3338
Size: 30 165 30 1085 30 655 30 135
Cuts at: 3338 3368 4030 4060 1178
Size: 30 662 30 4055
Fragments arranged by size:
4055 1085 662 655 165 135 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2324 2687 3766 3782 3872 3881 4013 4051
Size: 1138 363 1079 16 90 9 132 38
Cuts at: 4051 1186
Size: 4072
Fragments arranged by size:
4072 1138 1079 363 132 90 38 16
9
BseYI C'CCAG_C
Cuts at: 2474 2490 3050 3138 6140 6636 2474
Size: 16 560 88 3002 496 2775
Fragments arranged by size:
3002 2775 560 496 88 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2732 3317 3485 3566 3916 1163
Size: 573 996 585 168 81 350 4184
Fragments arranged by size:
4184 996 585 573 350 168 81
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3034 4387 5148 6176 1540
Size: 275 186 1033 1353 761 1028 2301
Fragments arranged by size:
2301 1353 1033 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2855 3060 3966 4998 5083 6154 2855
Size: 205 906 1032 85 1071 3638
Fragments arranged by size:
3638 1071 1032 906 205 85
BsmI GAATG_Cn'
Cuts at: 4448 4541 4448
Size: 93 6844
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2577 2661 2771 2855 2921 3060 3173
Size: 399 787 84 110 84 66 139 113
Cuts at: 3173 3774 3838 3894 3966 4139 4256 4998 5083
Size: 601 64 56 72 173 117 742 85
Cuts at: 5083 6154 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 787 742 601 399 173 139
117 113 110 85 84 84 72 66
64 56
BspEI T'CCGG_A
Cuts at: 6600 6600
Size: 6937
BspHI T'CATG_A
Cuts at: 4826 6556 4826
Size: 1730 5207
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2783 2898 3588 4292 844
Size: 1939 115 690 704 3489
Fragments arranged by size:
3489 1939 704 690 115
BsrBI CCG'CTC
Cuts at: 1394 1823 3685 4824 1394
Size: 429 1862 1139 3507
Fragments arranged by size:
3507 1862 1139 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5432 5606 65
Size: 1183 4184 174 1396
Fragments arranged by size:
4184 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2601 3740 3808 4085 5581 5768 1700
Size: 110 791 1139 68 277 1496 187 2869
Fragments arranged by size:
2869 1496 1139 791 277 187 110 68
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6937
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6937
BssSI C'ACGA_G
Cuts at: 3854 4997 6009 3854
Size: 1143 1012 4782
Fragments arranged by size:
4782 1143 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2859 3849 794
Size: 2065 990 3882
Fragments arranged by size:
3882 2065 990
BstBI TT'CG_AA
Cuts at: 2217 5741 2217
Size: 3524 3413
BstEII G'GTnAC_C
Cuts at: 1184 2809 4046 1184
Size: 1625 1237 4075
Fragments arranged by size:
4075 1625 1237
BstYI r'GATC_y
Cuts at: 1972 3943 4267 4634 5019 5036 6488 6574 6586
Size: 1971 324 367 385 17 1452 86 12
Cuts at: 6586 6932 1972
Size: 346 1977
Fragments arranged by size:
1977 1971 1452 385 367 346 324 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3749 3749
Size: 6937
BtgI C'CryG_G
Cuts at: 513 1065 2323 3034 513
Size: 552 1258 711 4416
Fragments arranged by size:
4416 1258 711 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6198
Fragments arranged by size:
6198 683 56
BtsI GCAGTG_nn'
Cuts at: 2083 2771 2857 3847 4227 4453 5247 5267 2083
Size: 688 86 990 380 226 794 20 3753
Fragments arranged by size:
3753 990 794 688 380 226 86 20
ClaI AT'CG_AT
Cuts at: 1554 2718 1554
Size: 1164 5773
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3514 3549 529
Size: 35 2950 35 3917
Fragments arranged by size:
3917 2950 35 35
DraI TTT'AAA
Cuts at: 4241 4241
Size: 6937
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5944 809
Size: 507 4628 1802
Fragments arranged by size:
4628 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3031 3387 4066
Size: 54 1425 50 275 18 1201 356 679
Cuts at: 4066 4384 5273 6902 8
Size: 318 889 1629 43
Fragments arranged by size:
1629 1425 1201 889 679 356 318 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3031 4384 1537
Size: 275 1219 1353 4090
Fragments arranged by size:
4090 1353 1219 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2237 2876 3140 3858 4028 4867 1353
Size: 884 639 264 718 170 839 3423
Fragments arranged by size:
3423 884 839 718 639 264 170
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5524 5898 6044 387
Size: 1064 138 3935 374 146 1280
Fragments arranged by size:
3935 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3058 3058
Size: 6937
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2581 2996 3164 3656 3779 4001 4217
Size: 393 949 415 168 492 123 222 216
Cuts at: 4217 5000 5597 6384 6652 1239
Size: 783 597 787 268 1524
Fragments arranged by size:
1524 949 787 783 597 492 415 393
268 222 216 168 123
EcoNI CCTnn'n_nnAGG
Cuts at: 2184 2184
Size: 6937
EcoO109I rG'GnC_Cy
Cuts at: 3169 3170 3688 4135 4136 3169
Size: 1 518 447 1 5970
Fragments arranged by size:
5970 518 447 1 1
EcoRI G'AATT_C
Cuts at: 3542 4246 4624 3542
Size: 704 378 5855
Fragments arranged by size:
5855 704 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4241 4241
Size: 6937
FspI TGC'GCA
Cuts at: 5443 5443
Size: 6937
HaeII r_GCGC'y
Cuts at: 1678 2481 2761 2983 3796 6084 1678
Size: 803 280 222 813 2288 2531
Fragments arranged by size:
2531 2288 813 803 280 222
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3932 3964 3998 4030 5578
Size: 32 135 32 2360 32 34 32 1548
Cuts at: 5578 5610 5652 5684 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2360 1548 135 42 34 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4281 4527 669
Size: 988 281 2343 246 3079
Fragments arranged by size:
3079 2343 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3527 748
Size: 2779 4158
HpaI GTT'AAC
Cuts at: 4527 4527
Size: 6937
KpnI G_GTAC'C
Cuts at: 4262 4262
Size: 6937
MfeI C'AATT_G
Cuts at: 4536 4536
Size: 6937
MluI A'CGCG_T
Cuts at: 2847 6813 2847
Size: 3966 2971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3939 6051 6235 1305
Size: 2634 2112 184 2007
Fragments arranged by size:
2634 2112 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3389 10
Size: 54 1425 343 1557 3558
Fragments arranged by size:
3558 1557 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2258 3084 3104 3336 3354 4173 4895
Size: 648 1092 826 20 232 18 819 722
Cuts at: 4895 5254 5413 518
Size: 359 159 2042
Fragments arranged by size:
2042 1092 826 819 722 648 359 232
159 20 18
MspA1I CmG'CkG
Cuts at: 1606 3036 3142 3226 4353 5030 6178 6423 1606
Size: 1430 106 84 1127 677 1148 245 2120
Fragments arranged by size:
2120 1430 1148 1127 677 245 106 84
NaeI GCC'GGC
Cuts at: 2603 4087 2603
Size: 1484 5453
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6385
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6937
NgoMIV G'CCGG_C
Cuts at: 2601 4085 2601
Size: 1484 5453
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6937
NotI GC'GGCC_GC
Cuts at: 3031 4384 3031
Size: 1353 5584
NsiI A_TGCA'T
Cuts at: 4015 4015
Size: 6937
NspI r_CATG'y
Cuts at: 1132 1229 2251 2905 3577 4295 5840 1132
Size: 97 1022 654 672 718 1545 2229
Fragments arranged by size:
2229 1545 1022 718 672 654 97
PasI CC'CwG_GG
Cuts at: 1348 3838 1348
Size: 2490 4447
PciI A'CATG_T
Cuts at: 5836 5836
Size: 6937
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2821 3706 1108
Size: 1713 885 4339
Fragments arranged by size:
4339 1713 885
PfoI T'CCnGG_A
Cuts at: 2136 2136
Size: 6937
PmeI GTTT'AAAC
Cuts at: 4241 4241
Size: 6937
PmlI CAC'GTG
Cuts at: 3406 3406
Size: 6937
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5006 5038 6519 6551 5006
Size: 32 1481 32 5392
Fragments arranged by size:
5392 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6937
PsiI TTA'TAA
Cuts at: 4507 4507
Size: 6937
PspOMI G'GGCC_C
Cuts at: 3169 4135 3169
Size: 966 5971
PstI C_TGCA'G
Cuts at: 830 2094 2281 3231 4289 830
Size: 1264 187 950 1058 3478
Fragments arranged by size:
3478 1264 1058 950 187
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6937
PvuII CAG'CTG
Cuts at: 3142 3226 3142
Size: 84 6853
SacI G_AGCT'C
Cuts at: 3060 3060
Size: 6937
SacII CC_GC'GG
Cuts at: 3037 3037
Size: 6937
SalI G'TCGA_C
Cuts at: 1936 4279 1936
Size: 2343 4594
SapI GCTCTTCn'nnn_
Cuts at: 2237 2876 2237
Size: 639 6298
SbfI CC_TGCA'GG
Cuts at: 4289 4289
Size: 6937
ScaI AGT'ACT
Cuts at: 5185 6897 5185
Size: 1712 5225
SexAI A'CCwGG_T
Cuts at: 2805 2805
Size: 6937
SfcI C'TryA_G
Cuts at: 826 1045 2090 2277 3227 3895 4285 5420 6101
Size: 219 1045 187 950 668 390 1135 681
Cuts at: 6101 6292 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1045 950 681 668 390 219
191 187
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6937
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6937
SmaI CCC'GGG
Cuts at: 1872 2209 2550 4264 1872
Size: 337 341 1714 4545
Fragments arranged by size:
4545 1714 341 337
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3059 3311 3479 5041 5942 6204
Size: 197 926 1116 252 168 1562 901 262
Cuts at: 6204 820
Size: 1553
Fragments arranged by size:
1562 1553 1116 926 901 262 252 197
168
SnaBI TAC'GTA
Cuts at: 493 4728 493
Size: 4235 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6937
SphI G_CATG'C
Cuts at: 1132 2251 4295 1132
Size: 1119 2044 3774
Fragments arranged by size:
3774 2044 1119
SspI AAT'ATT
Cuts at: 5 52 4861 5
Size: 47 4809 2081
Fragments arranged by size:
4809 2081 47
StuI AGG'CCT
Cuts at: 1517 2954 1517
Size: 1437 5500
StyI C'CwwG_G
Cuts at: 513 1065 3132 3700 3801 4017 4051 4376 513
Size: 552 2067 568 101 216 34 325 3074
Fragments arranged by size:
3074 2067 568 552 325 216 101 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2828 2975 3344 4964 4981 5134 5319
Size: 1255 1248 147 369 1620 17 153 185
Cuts at: 5319 5764 325
Size: 445 1498
Fragments arranged by size:
1620 1498 1255 1248 445 369 185 153
147 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2799 3180 5183
Size: 274 80 33 51 1443 822 381 2003
Cuts at: 5183 6895 96
Size: 1712 138
Fragments arranged by size:
2003 1712 1443 822 381 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3338 3370 4030 4062 6897 6929 3338
Size: 32 660 32 2835 32 3346
Fragments arranged by size:
3346 2835 660 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6937
XbaI T'CTAG_A
Cuts at: 4273 4273
Size: 6937
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2686 2803 2686
Size: 117 6820
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6937
XmaI C'CCGG_G
Cuts at: 1870 2207 2548 4262 1870
Size: 337 341 1714 4545
Fragments arranged by size:
4545 1714 341 337
XmnI GAAnn'nnTTC
Cuts at: 2870 3515 3542 5066 2870
Size: 645 27 1524 4741
Fragments arranged by size:
4741 1524 645 27
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4745 276
Size: 53 83 186 936 3211 2468
Fragments arranged by size:
3211 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI
KasI NarI NruI PacI PpuMI PspXI PsrI RsrII
SanDI SfiI SfoI SrfI SwaI