Restriction map for
pFN21AB8418
(Circular) (Six-base) MAPSORT of: pFN21AB8418.seq Check: 4902 from: 1 to: 7132
pFN21AB8418 7132 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4942 278
Size: 53 83 186 936 3406 2468
Fragments arranged by size:
3406 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4475 1937
Size: 2538 4594
Acc65I G'GTAC_C
Cuts at: 4453 4453
Size: 7132
AclI AA'CG_TT
Cuts at: 1563 5259 5632 1563
Size: 3696 373 3063
Fragments arranged by size:
3696 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4401 5195 6579 6847 1632
Size: 2769 794 1384 268 1917
Fragments arranged by size:
2769 1917 1384 794 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6935
AflIII A'CryG_T
Cuts at: 1203 2696 3032 6031 7008 1203
Size: 1493 336 2999 977 1327
Fragments arranged by size:
2999 1493 1327 977 336
AgeI A'CCGG_T
Cuts at: 5963 5963
Size: 7132
AhdI GACnn_n'nnGTC
Cuts at: 5861 5861
Size: 7132
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7132
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7132
AlwNI CAG_nnn'CTG
Cuts at: 4875 6447 4875
Size: 1572 5560
ApaLI G'TGCA_C
Cuts at: 2381 5189 6345 2381
Size: 2808 1156 3168
Fragments arranged by size:
3168 2808 1156
ApoI r'AATT_y
Cuts at: 1493 2039 2083 2474 2482 2602 2855 2922 3006
Size: 546 44 391 8 120 253 67 84
Cuts at: 3006 3107 3174 3342 3426 3510 3762 3779 3846
Size: 101 67 168 84 84 252 17 67
Cuts at: 3846 4182 4266 4350 4441 4667 4819 6928 1493
Size: 336 84 84 91 226 152 2109 1697
Fragments arranged by size:
2109 1697 546 391 336 253 252 226
168 152 120 101 91 84 84 84
84 84 67 67 67 44 17 8
AseI AT'TA_AT
Cuts at: 160 1032 2673 5686 160
Size: 872 1641 3013 1606
Fragments arranged by size:
3013 1641 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7132
AvaI C'yCGr_G
Cuts at: 1870 1943 4457 1870
Size: 73 2514 4545
Fragments arranged by size:
4545 2514 73
BamHI G'GATC_C
Cuts at: 4462 4829 4462
Size: 367 6765
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4453 5908 618
Size: 325 217 627 2666 1455 1842
Fragments arranged by size:
2666 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4451 1391
Size: 3060 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2119 928
Size: 971 220 5941
Fragments arranged by size:
5941 971 220
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5323 5357 1903
Size: 34 3386 34 3678
Fragments arranged by size:
3678 3386 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5024 6234 5024
Size: 1210 5922
BclI T'GATC_A
Cuts at: 1548 2160 1548
Size: 612 6520
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5743 136
Size: 107 122 71 631 605 4071 1525
Fragments arranged by size:
4071 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7127 7127
Size: 7132
BlpI GC'TnA_GC
Cuts at: 4549 4549
Size: 7132
Bme1580I G_kGCm'C
Cuts at: 1790 2385 5193 6349 1790
Size: 595 2808 1156 2573
Fragments arranged by size:
2808 2573 1156 595
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7132
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2225 4863 5821
Size: 416 53 83 399 24 808 2638 958
Cuts at: 5821 442
Size: 1753
Fragments arranged by size:
2638 1753 958 808 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7132
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4385 4385
Size: 7132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 2192 2700 2868 2952 3036 3288 3372
Size: 830 123 508 168 84 84 252 84
Cuts at: 3372 3624 3708 3792 3876 4044 4128 4212 4380
Size: 252 84 84 84 168 84 84 168
Cuts at: 4380 5792 1239
Size: 1412 2579
Fragments arranged by size:
2579 1412 830 508 252 252 168 168
168 123 84 84 84 84 84 84
84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5257 6122 6420 5257
Size: 865 298 5969
Fragments arranged by size:
5969 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5795 882
Size: 617 4296 2219
Fragments arranged by size:
4296 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4923 493
Size: 711 3719 2702
Fragments arranged by size:
3719 2702 711
BsaBI GATnn'nnATC
Cuts at: 7126 7126
Size: 7132
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4939 5321 5956 275
Size: 53 83 186 936 3406 382 635 1451
Fragments arranged by size:
3406 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5565 5963 6237 6384 6795 5565
Size: 398 274 147 411 5902
Fragments arranged by size:
5902 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6907
Fragments arranged by size:
6907 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 2218 2403 3956 1186
Size: 880 152 185 1553 4362
Fragments arranged by size:
4362 1553 880 185 152
BseYI C'CCAG_C
Cuts at: 6335 6831 6335
Size: 496 6636
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6559
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4582 5343 6371 1540
Size: 275 186 2581 761 1028 2301
Fragments arranged by size:
2581 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2385 5193 5278 6349 2385
Size: 2808 85 1071 3168
Fragments arranged by size:
3168 2808 1071 85
BsmI GAATG_Cn'
Cuts at: 4643 4736 4643
Size: 93 7039
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2385 4451 5193 5278 6349 1391
Size: 399 595 2066 742 85 1071 2174
Fragments arranged by size:
2174 2066 1071 742 595 399 85
BspEI T'CCGG_A
Cuts at: 6795 6795
Size: 7132
BspHI T'CATG_A
Cuts at: 5021 6751 5021
Size: 1730 5402
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4487 844
Size: 3643 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5019 1394
Size: 429 3196 3507
Fragments arranged by size:
3507 3196 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2066 5627 5801 65
Size: 1183 818 3561 174 1396
Fragments arranged by size:
3561 1396 1183 818 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3144 3480 5776 5963 1700
Size: 110 1334 336 2296 187 2869
Fragments arranged by size:
2869 2296 1334 336 187 110
BsrGI T'GTAC_A
Cuts at: 96 2338 3119 96
Size: 2242 781 4109
Fragments arranged by size:
4109 2242 781
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7132
BssSI C'ACGA_G
Cuts at: 5192 6204 5192
Size: 1012 6120
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7132
BstBI TT'CG_AA
Cuts at: 5936 5936
Size: 7132
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7132
BstYI r'GATC_y
Cuts at: 1972 2284 4462 4829 5214 5231 6683 6769 6781
Size: 312 2178 367 385 17 1452 86 12
Cuts at: 6781 7127 1972
Size: 346 1977
Fragments arranged by size:
2178 1977 1452 385 367 346 312 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5641
Fragments arranged by size:
5641 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2056 487
Size: 683 56 830 5563
Fragments arranged by size:
5563 830 683 56
BtsI GCAGTG_nn'
Cuts at: 4648 5442 5462 4648
Size: 794 20 6318
Fragments arranged by size:
6318 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7132
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2521 2556 529
Size: 35 1957 35 5105
Fragments arranged by size:
5105 1957 35 35
DraI TTT'AAA
Cuts at: 2346 4066 4436 2346
Size: 1720 370 5042
Fragments arranged by size:
5042 1720 370
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6139 809
Size: 507 4823 1802
Fragments arranged by size:
4823 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2264 4579
Size: 54 1425 50 275 18 201 233 2315
Cuts at: 4579 5468 7097 8
Size: 889 1629 43
Fragments arranged by size:
2315 1629 1425 889 275 233 201 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4579 1537
Size: 275 2767 4090
Fragments arranged by size:
4090 2767 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5062 1353
Size: 3709 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5719 6093 6239 387
Size: 1064 138 4130 374 146 1280
Fragments arranged by size:
4130 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2192 2700 2868 2952 3036 3288
Size: 393 437 123 508 168 84 84 252
Cuts at: 3288 3372 3624 3708 3792 3876 4044 4128 4212
Size: 84 252 84 84 84 168 84 84
Cuts at: 4212 4380 4401 5195 5792 6579 6847 1239
Size: 168 21 794 597 787 268 1524
Fragments arranged by size:
1524 794 787 597 508 437 393 268
252 252 168 168 168 123 84 84
84 84 84 84 84 84 21
EcoRI G'AATT_C
Cuts at: 2039 3006 3342 3426 4266 4441 4819 2039
Size: 967 336 84 840 175 378 4352
Fragments arranged by size:
4352 967 840 378 336 175 84
FspI TGC'GCA
Cuts at: 5638 5638
Size: 7132
HaeII r_GCGC'y
Cuts at: 1678 6279 1678
Size: 4601 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2152 2184 2757 2789 4393
Size: 32 135 32 580 32 573 32 1604
Cuts at: 4393 4425 5773 5805 5847 5879 1373
Size: 32 1348 32 42 32 2626
Fragments arranged by size:
2626 1604 1348 580 573 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3258 4476 4722 669
Size: 988 281 1320 1218 246 3079
Fragments arranged by size:
3079 1320 1218 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2631 2799 2883 3135 3219 3239 3471 3555
Size: 1883 168 84 252 84 20 232 84
Cuts at: 3555 3575 3639 3723 3807 3891 4059 4143 4311
Size: 20 64 84 84 84 168 84 168
Cuts at: 4311 748
Size: 3569
Fragments arranged by size:
3569 1883 252 232 168 168 168 84
84 84 84 84 84 84 64 20
20
HpaI GTT'AAC
Cuts at: 3258 4722 3258
Size: 1464 5668
KpnI G_GTAC'C
Cuts at: 4457 4457
Size: 7132
MfeI C'AATT_G
Cuts at: 4731 4731
Size: 7132
MluI A'CGCG_T
Cuts at: 7008 7008
Size: 7132
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6246 6430 1305
Size: 4941 184 2007
Fragments arranged by size:
4941 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2033 2266 10
Size: 54 1425 343 201 233 4876
Fragments arranged by size:
4876 1425 343 233 201 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2334 2361 5090 5449 5608 518
Size: 648 1168 27 2729 359 159 2042
Fragments arranged by size:
2729 2042 1168 648 359 159 27
MspA1I CmG'CkG
Cuts at: 1606 4548 5225 6373 6618 1606
Size: 2942 677 1148 245 2120
Fragments arranged by size:
2942 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5641
Fragments arranged by size:
5641 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7132
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7132
NotI GC'GGCC_GC
Cuts at: 4579 4579
Size: 7132
NspI r_CATG'y
Cuts at: 1132 1229 2560 2700 3036 4490 6035 1132
Size: 97 1331 140 336 1454 1545 2229
Fragments arranged by size:
2229 1545 1454 1331 336 140 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7132
PciI A'CATG_T
Cuts at: 2696 3032 6031 2696
Size: 336 2999 3797
Fragments arranged by size:
3797 2999 336
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7132
PfoI T'CCnGG_A
Cuts at: 2929 2929
Size: 7132
PmeI GTTT'AAAC
Cuts at: 4436 4436
Size: 7132
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5201 5233 6714 6746 5201
Size: 32 1481 32 5587
Fragments arranged by size:
5587 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7132
PsiI TTA'TAA
Cuts at: 2397 4702 2397
Size: 2305 4827
PstI C_TGCA'G
Cuts at: 830 3103 3439 4484 830
Size: 2273 336 1045 3478
Fragments arranged by size:
3478 2273 1045 336
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7132
SalI G'TCGA_C
Cuts at: 1936 4474 1936
Size: 2538 4594
SbfI CC_TGCA'GG
Cuts at: 4484 4484
Size: 7132
ScaI AGT'ACT
Cuts at: 5380 7092 5380
Size: 1712 5420
SexAI A'CCwGG_T
Cuts at: 2121 2121
Size: 7132
SfcI C'TryA_G
Cuts at: 826 1045 2113 3099 3435 4480 5615 6296 6487
Size: 219 1068 986 336 1045 1135 681 191
Cuts at: 6487 826
Size: 1471
Fragments arranged by size:
1471 1135 1068 1045 986 681 336 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7132
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7132
SmaI CCC'GGG
Cuts at: 1872 4459 1872
Size: 2587 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5236 6137 6399 820
Size: 197 926 3293 901 262 1553
Fragments arranged by size:
3293 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4923 493
Size: 4430 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7132
SphI G_CATG'C
Cuts at: 1132 2560 4490 1132
Size: 1428 1930 3774
Fragments arranged by size:
3774 1930 1428
SspI AAT'ATT
Cuts at: 5 52 2439 4325 5056 5
Size: 47 2387 1886 731 2081
Fragments arranged by size:
2387 2081 1886 731 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7132
StyI C'CwwG_G
Cuts at: 513 1065 2004 2189 4571 513
Size: 552 939 185 2382 3074
Fragments arranged by size:
3074 2382 939 552 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5159 5176 5329 5514 5959 325
Size: 1255 3579 17 153 185 445 1498
Fragments arranged by size:
3579 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2338 3119 5378
Size: 274 80 33 51 1443 361 781 2259
Cuts at: 5378 7090 96
Size: 1712 138
Fragments arranged by size:
2259 1712 1443 781 361 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7092 7124 7092
Size: 32 7100
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7132
XbaI T'CTAG_A
Cuts at: 4468 4468
Size: 7132
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7132
XmaI C'CCGG_G
Cuts at: 1870 4457 1870
Size: 2587 4545
XmnI GAAnn'nnTTC
Cuts at: 5261 5261
Size: 7132
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4940 276
Size: 53 83 186 936 3406 2468
Fragments arranged by size:
3406 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI XcmI