Restriction map for
pFN21AB8417
(Circular) (Six-base) MAPSORT of: pFN21AB8417.seq Check: 6408 from: 1 to: 7060
pFN21AB8417 7060 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4870 278
Size: 53 83 186 936 3334 2468
Fragments arranged by size:
3334 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4403 1937
Size: 2466 4594
Acc65I G'GTAC_C
Cuts at: 4381 4381
Size: 7060
AclI AA'CG_TT
Cuts at: 1563 5187 5560 1563
Size: 3624 373 3063
Fragments arranged by size:
3624 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2728 3087 3588 5123 6507 6775 1632
Size: 1096 359 501 1535 1384 268 1917
Fragments arranged by size:
1917 1535 1384 1096 501 359 268
AflII C'TTAA_G
Cuts at: 820 1017 3872 820
Size: 197 2855 4008
Fragments arranged by size:
4008 2855 197
AflIII A'CryG_T
Cuts at: 1203 2251 3432 5959 6936 1203
Size: 1048 1181 2527 977 1327
Fragments arranged by size:
2527 1327 1181 1048 977
AgeI A'CCGG_T
Cuts at: 5891 5891
Size: 7060
AhdI GACnn_n'nnGTC
Cuts at: 5789 5789
Size: 7060
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7060
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7060
AlwNI CAG_nnn'CTG
Cuts at: 3203 4803 6375 3203
Size: 1600 1572 3888
Fragments arranged by size:
3888 1600 1572
ApaLI G'TGCA_C
Cuts at: 5117 6273 5117
Size: 1156 5904
ApoI r'AATT_y
Cuts at: 1493 2797 4369 4595 4747 6856 1493
Size: 1304 1572 226 152 2109 1697
Fragments arranged by size:
2109 1697 1572 1304 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3297 5614 160
Size: 872 2265 2317 1606
Fragments arranged by size:
2317 2265 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7060
AvaI C'yCGr_G
Cuts at: 1870 1943 4385 1870
Size: 73 2442 4545
Fragments arranged by size:
4545 2442 73
BamHI G'GATC_C
Cuts at: 4390 4757 4390
Size: 367 6693
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 4381 5836 618
Size: 325 217 627 221 2373 1455 1842
Fragments arranged by size:
2373 1842 1455 627 325 221 217
BanII G_rGCy'C
Cuts at: 1391 2136 3796 4379 1391
Size: 745 1660 583 4072
Fragments arranged by size:
4072 1660 745 583
BbeI G_GCGC'C
Cuts at: 2012 2012
Size: 7060
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2734 2861 3234 928
Size: 971 835 127 373 4754
Fragments arranged by size:
4754 971 835 373 127
BbvCI CC'TCA_GC
Cuts at: 3888 3888
Size: 7060
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5251 5285 1903
Size: 34 3314 34 3678
Fragments arranged by size:
3678 3314 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2848 3026 3491 4952 6162 2848
Size: 178 465 1461 1210 3746
Fragments arranged by size:
3746 1461 1210 465 178
BclI T'GATC_A
Cuts at: 1548 2164 1548
Size: 616 6444
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2524 5671 136
Size: 107 122 71 631 605 852 3147 1525
Fragments arranged by size:
3147 1525 852 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2749 3981 7055 2749
Size: 1232 3074 2754
Fragments arranged by size:
3074 2754 1232
BlpI GC'TnA_GC
Cuts at: 4477 4477
Size: 7060
Bme1580I G_kGCm'C
Cuts at: 1790 3961 5121 6277 1790
Size: 2171 1160 1156 2573
Fragments arranged by size:
2573 2171 1160 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7060
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2228 3542 4791
Size: 416 53 83 399 24 811 1314 1249
Cuts at: 4791 5749 442
Size: 958 1753
Fragments arranged by size:
1753 1314 1249 958 811 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7060
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2074 2074
Size: 7060
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2992 5720 1239
Size: 1753 2728 2579
Fragments arranged by size:
2728 2579 1753
Bpu10I CC'TnA_GC
Cuts at: 2225 3888 2225
Size: 1663 5397
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2105 3549 5185 6050 6348 2105
Size: 1444 1636 865 298 2817
Fragments arranged by size:
2817 1636 1444 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5723 882
Size: 617 4224 2219
Fragments arranged by size:
4224 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4851 493
Size: 711 3647 2702
Fragments arranged by size:
3647 2702 711
BsaBI GATnn'nnATC
Cuts at: 2277 7054 2277
Size: 4777 2283
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3126 4867 5249
Size: 53 83 186 936 476 1117 1741 382
Cuts at: 5249 5884 275
Size: 635 1451
Fragments arranged by size:
1741 1451 1117 936 635 476 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2382 3819 5493 5891 6165 6312 6723 2382
Size: 1437 1674 398 274 147 411 2719
Fragments arranged by size:
2719 1674 1437 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4029 4059 1178
Size: 30 165 30 2626 30 4179
Fragments arranged by size:
4179 2626 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2577 1186
Size: 1391 5669
BseYI C'CCAG_C
Cuts at: 3960 6263 6759 3960
Size: 2303 496 4261
Fragments arranged by size:
4261 2303 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3264 1163
Size: 573 1528 4959
Fragments arranged by size:
4959 1528 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4510 5271 6299 1540
Size: 275 186 2509 761 1028 2301
Fragments arranged by size:
2509 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5121 5206 6277 5121
Size: 85 1071 5904
Fragments arranged by size:
5904 1071 85
BsmI GAATG_Cn'
Cuts at: 3254 4571 4664 3254
Size: 1317 93 5650
Fragments arranged by size:
5650 1317 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 3796 3961 4379 5121 5206 6277
Size: 399 346 1660 165 418 742 85 1071
Cuts at: 6277 1391
Size: 2174
Fragments arranged by size:
2174 1660 1071 742 418 399 346 165
85
BspEI T'CCGG_A
Cuts at: 6723 6723
Size: 7060
BspHI T'CATG_A
Cuts at: 4949 6679 4949
Size: 1730 5330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4415 844
Size: 3571 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4947 1394
Size: 429 3124 3507
Fragments arranged by size:
3507 3124 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2551 5555 5729 65
Size: 1183 1303 3004 174 1396
Fragments arranged by size:
3004 1396 1303 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5704 5891 1700
Size: 110 3894 187 2869
Fragments arranged by size:
3894 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3973 96
Size: 3877 3183
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7060
BssSI C'ACGA_G
Cuts at: 3977 5120 6132 3977
Size: 1143 1012 4905
Fragments arranged by size:
4905 1143 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2095 3599 794
Size: 1301 1504 4255
Fragments arranged by size:
4255 1504 1301
BstBI TT'CG_AA
Cuts at: 5864 5864
Size: 7060
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7060
BstXI CCAn_nnnn'nTGG
Cuts at: 3509 3509
Size: 7060
BstYI r'GATC_y
Cuts at: 1972 2749 3981 4390 4757 5142 5159 6611 6697
Size: 777 1232 409 367 385 17 1452 86
Cuts at: 6697 6709 7055 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1232 777 409 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2707 513
Size: 552 939 703 4866
Fragments arranged by size:
4866 939 703 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6321
Fragments arranged by size:
6321 683 56
BtsI GCAGTG_nn'
Cuts at: 4576 5370 5390 4576
Size: 794 20 6246
Fragments arranged by size:
6246 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7060
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7025
DraI TTT'AAA
Cuts at: 4191 4338 4364 4191
Size: 147 26 6887
Fragments arranged by size:
6887 147 26
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6067 809
Size: 507 4751 1802
Fragments arranged by size:
4751 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 4507 5396
Size: 54 1425 50 275 18 234 2443 889
Cuts at: 5396 7025 8
Size: 1629 43
Fragments arranged by size:
2443 1629 1425 889 275 234 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4507 1537
Size: 275 2695 4090
Fragments arranged by size:
4090 2695 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2141 2916 4990 1353
Size: 788 775 2074 3423
Fragments arranged by size:
3423 2074 788 775
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5647 6021 6167 387
Size: 1064 138 4058 374 146 1280
Fragments arranged by size:
4058 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2728 2992 3087 3588 5123 5720 6507
Size: 393 1096 264 95 501 1535 597 787
Cuts at: 6507 6775 1239
Size: 268 1524
Fragments arranged by size:
1535 1524 1096 787 597 501 393 268
264 95
EcoNI CCTnn'n_nnAGG
Cuts at: 3027 3027
Size: 7060
EcoO109I rG'GnC_Cy
Cuts at: 3020 3884 3020
Size: 864 6196
EcoRI G'AATT_C
Cuts at: 2797 4369 4747 2797
Size: 1572 378 5110
Fragments arranged by size:
5110 1572 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2431 3092 2431
Size: 661 6399
FspI TGC'GCA
Cuts at: 5566 5566
Size: 7060
HaeII r_GCGC'y
Cuts at: 1678 2012 6207 1678
Size: 334 4195 2531
Fragments arranged by size:
4195 2531 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2941 2973 2990 3022 5701
Size: 32 135 32 1369 32 17 32 2679
Cuts at: 5701 5733 5775 5807 1373
Size: 32 42 32 2626
Fragments arranged by size:
2679 2626 1369 135 42 32 32 32
32 32 32 17
HincII GTy'rAC
Cuts at: 669 1657 1938 4035 4404 4650 669
Size: 988 281 2097 369 246 3079
Fragments arranged by size:
3079 2097 988 369 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7060
HpaI GTT'AAC
Cuts at: 4035 4650 4035
Size: 615 6445
KasI G'GCGC_C
Cuts at: 2008 2008
Size: 7060
KpnI G_GTAC'C
Cuts at: 4385 4385
Size: 7060
MfeI C'AATT_G
Cuts at: 2802 4003 4659 2802
Size: 1201 656 5203
Fragments arranged by size:
5203 1201 656
MluI A'CGCG_T
Cuts at: 6936 6936
Size: 7060
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3580 4200 6174 6358 1305
Size: 2275 620 1974 184 2007
Fragments arranged by size:
2275 2007 1974 620 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2066 10
Size: 54 1425 343 234 5004
Fragments arranged by size:
5004 1425 343 234 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5018 5377 5536 518
Size: 648 3852 359 159 2042
Fragments arranged by size:
3852 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4261 4298 4476 5153 6301 6546 1606
Size: 2655 37 178 677 1148 245 2120
Fragments arranged by size:
2655 2120 1148 677 245 178 37
NarI GG'CG_CC
Cuts at: 2009 2009
Size: 7060
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5569
Fragments arranged by size:
5569 939 552
NdeI CA'TA_TG
Cuts at: 387 2161 387
Size: 1774 5286
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7060
NotI GC'GGCC_GC
Cuts at: 4507 4507
Size: 7060
NspI r_CATG'y
Cuts at: 1132 1229 2255 2762 3436 4418 5963 1132
Size: 97 1026 507 674 982 1545 2229
Fragments arranged by size:
2229 1545 1026 982 674 507 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7060
PciI A'CATG_T
Cuts at: 2251 3432 5959 2251
Size: 1181 2527 3352
Fragments arranged by size:
3352 2527 1181
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7060
PmeI GTTT'AAAC
Cuts at: 4364 4364
Size: 7060
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5129 5161 6642 6674 5129
Size: 32 1481 32 5515
Fragments arranged by size:
5515 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3020 3884 3020
Size: 864 6196
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7060
PsiI TTA'TAA
Cuts at: 3102 3665 3738 4269 4630 3102
Size: 563 73 531 361 5532
Fragments arranged by size:
5532 563 531 361 73
PstI C_TGCA'G
Cuts at: 830 4412 830
Size: 3582 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7060
PvuII CAG'CTG
Cuts at: 4298 4298
Size: 7060
SalI G'TCGA_C
Cuts at: 1936 4402 1936
Size: 2466 4594
SapI GCTCTTCn'nnn_
Cuts at: 2141 2141
Size: 7060
SbfI CC_TGCA'GG
Cuts at: 4412 4412
Size: 7060
ScaI AGT'ACT
Cuts at: 2094 5308 7020 2094
Size: 3214 1712 2134
Fragments arranged by size:
3214 2134 1712
SfcI C'TryA_G
Cuts at: 826 1045 2028 3396 3570 4408 5543 6224 6415
Size: 219 983 1368 174 838 1135 681 191
Cuts at: 6415 826
Size: 1471
Fragments arranged by size:
1471 1368 1135 983 838 681 219 191
174
SfoI GGC'GCC
Cuts at: 2010 2010
Size: 7060
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7060
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7060
SmaI CCC'GGG
Cuts at: 1872 4387 1872
Size: 2515 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2120 3564 3872 5164 6065 6327
Size: 197 926 177 1444 308 1292 901 262
Cuts at: 6327 820
Size: 1553
Fragments arranged by size:
1553 1444 1292 926 901 308 262 197
177
SnaBI TAC'GTA
Cuts at: 493 4851 493
Size: 4358 2702
SpeI A'CTAG_T
Cuts at: 152 3143 4174 152
Size: 2991 1031 3038
Fragments arranged by size:
3038 2991 1031
SphI G_CATG'C
Cuts at: 1132 2762 4418 1132
Size: 1630 1656 3774
Fragments arranged by size:
3774 1656 1630
SspI AAT'ATT
Cuts at: 5 52 4104 4984 5
Size: 47 4052 880 2081
Fragments arranged by size:
4052 2081 880 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7060
StyI C'CwwG_G
Cuts at: 513 1065 2004 2128 2324 4063 4499 513
Size: 552 939 124 196 1739 436 3074
Fragments arranged by size:
3074 1739 939 552 436 196 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2593 3387 4292 5087 5104 5257 5442
Size: 1255 1013 794 905 795 17 153 185
Cuts at: 5442 5887 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1013 905 795 794 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2092 2594 2644
Size: 274 80 33 51 1443 115 502 50
Cuts at: 2644 3439 3832 3973 5306 7018 96
Size: 795 393 141 1333 1712 138
Fragments arranged by size:
1712 1443 1333 795 502 393 274 141
138 115 80 51 50 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4029 4061 7020 7052 4029
Size: 32 2959 32 4037
Fragments arranged by size:
4037 2959 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7060
XbaI T'CTAG_A
Cuts at: 4396 4396
Size: 7060
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7060
XmaI C'CCGG_G
Cuts at: 1870 4385 1870
Size: 2515 4545
XmnI GAAnn'nnTTC
Cuts at: 2910 3072 5189 2910
Size: 162 2117 4781
Fragments arranged by size:
4781 2117 162
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4868 276
Size: 53 83 186 936 3334 2468
Fragments arranged by size:
3334 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SrfI SwaI XcmI