Restriction map for
pFN21AB8414
(Circular) (Six-base) MAPSORT of: pFN21AB8414.seq Check: 4345 from: 1 to: 7444
pFN21AB8414 7444 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3161 5254 278
Size: 53 83 186 936 1625 2093 2468
Fragments arranged by size:
2468 2093 1625 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2106 2372 4787 1937
Size: 169 266 2415 4594
Fragments arranged by size:
4594 2415 266 169
Acc65I G'GTAC_C
Cuts at: 3042 4765 3042
Size: 1723 5721
AclI AA'CG_TT
Cuts at: 1563 5571 5944 1563
Size: 4008 373 3063
Fragments arranged by size:
4008 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2228 2341 3462 4470 5507 6891 7159 1632
Size: 596 113 1121 1008 1037 1384 268 1917
Fragments arranged by size:
1917 1384 1121 1037 1008 596 268 113
AfeI AGC'GCT
Cuts at: 4058 4058
Size: 7444
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7247
AflIII A'CryG_T
Cuts at: 1203 3402 4241 4727 6343 7320 1203
Size: 2199 839 486 1616 977 1327
Fragments arranged by size:
2199 1616 1327 977 839 486
AgeI A'CCGG_T
Cuts at: 6275 6275
Size: 7444
AhdI GACnn_n'nnGTC
Cuts at: 6173 6173
Size: 7444
AleI CACnn'nnGTG
Cuts at: 1166 2421 3405 3800 1166
Size: 1255 984 395 4810
Fragments arranged by size:
4810 1255 984 395
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3818 3925 1184
Size: 2634 107 4703
Fragments arranged by size:
4703 2634 107
AlwNI CAG_nnn'CTG
Cuts at: 2672 4011 4394 5187 6759 2672
Size: 1339 383 793 1572 3357
Fragments arranged by size:
3357 1572 1339 793 383
ApaI G_GGCC'C
Cuts at: 2206 2825 2206
Size: 619 6825
ApaLI G'TGCA_C
Cuts at: 2798 5501 6657 2798
Size: 2703 1156 3585
Fragments arranged by size:
3585 2703 1156
ApoI r'AATT_y
Cuts at: 1493 3827 4250 4753 4979 5131 7240 1493
Size: 2334 423 503 226 152 2109 1697
Fragments arranged by size:
2334 2109 1697 503 423 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5998 160
Size: 872 4966 1606
Fragments arranged by size:
4966 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7444
AvaI C'yCGr_G
Cuts at: 1870 1943 2032 2069 2260 4769 1870
Size: 73 89 37 191 2509 4545
Fragments arranged by size:
4545 2509 191 89 73 37
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3026 3059 3026
Size: 33 7411
BamHI G'GATC_C
Cuts at: 2310 4295 4774 5141 2310
Size: 1985 479 367 4613
Fragments arranged by size:
4613 1985 479 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3042 3794 4765 6220 618
Size: 325 217 627 1255 752 971 1455 1842
Fragments arranged by size:
1842 1455 1255 971 752 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2206 2825 4763 1391
Size: 815 619 1938 4072
Fragments arranged by size:
4072 1938 815 619
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3873 928
Size: 971 1974 4499
Fragments arranged by size:
4499 1974 971
BbvCI CC'TCA_GC
Cuts at: 3166 3166
Size: 7444
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2627 2661 2664 2698 5635 5669 1903
Size: 34 690 34 3 34 2937 34 3678
Fragments arranged by size:
3678 2937 690 34 34 34 34 3
BciVI GTATCCnnnnn_n'
Cuts at: 4127 4189 4490 5336 6546 4127
Size: 62 301 846 1210 5025
Fragments arranged by size:
5025 1210 846 301 62
BclI T'GATC_A
Cuts at: 1548 3631 1548
Size: 2083 5361
BfrBI ATG'CAT
Cuts at: 4116 4116
Size: 7444
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3176 4032 6055
Size: 107 122 71 631 605 1504 856 2023
Cuts at: 6055 136
Size: 1525
Fragments arranged by size:
2023 1525 1504 856 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2542 3121 7439 2542
Size: 579 4318 2547
Fragments arranged by size:
4318 2547 579
BlpI GC'TnA_GC
Cuts at: 4861 4861
Size: 7444
Bme1580I G_kGCm'C
Cuts at: 1790 2206 2368 2802 2825 4075 5505 6661 1790
Size: 416 162 434 23 1250 1430 1156 2573
Fragments arranged by size:
2573 1430 1250 1156 434 416 162 23
BmgBI CAC'GTC
Cuts at: 1318 2581 3526 4536 1318
Size: 1263 945 1010 4226
Fragments arranged by size:
4226 1263 1010 945
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2128 2362 3162
Size: 416 53 83 399 24 711 234 800
Cuts at: 3162 3667 3715 5175 6133 442
Size: 505 48 1460 958 1753
Fragments arranged by size:
1753 1460 958 800 711 505 416 399
234 83 53 48 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7444
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2676 3247 3539 3741 4003 6104 1239
Size: 1437 571 292 202 262 2101 2579
Fragments arranged by size:
2579 2101 1437 571 292 262 202
Bpu10I CC'TnA_GC
Cuts at: 3166 3740 3166
Size: 574 6870
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5569 6434 6732 5569
Size: 865 298 6281
Fragments arranged by size:
6281 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3558 3609 6107 882
Size: 617 2059 51 2498 2219
Fragments arranged by size:
2498 2219 2059 617 51
BsaAI yAC'GTr
Cuts at: 493 1204 2797 3403 5235 493
Size: 711 1593 606 1832 2702
Fragments arranged by size:
2702 1832 1593 711 606
BsaBI GATnn'nnATC
Cuts at: 4501 7438 4501
Size: 2937 4507
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3158 5251 5633 6268
Size: 53 83 186 936 1625 2093 382 635
Cuts at: 6268 275
Size: 1451
Fragments arranged by size:
2093 1625 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5877 6275 6549 6696 7107 5877
Size: 398 274 147 411 6214
Fragments arranged by size:
6214 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2951 2981 2987 3017 3782
Size: 30 165 30 1548 30 6 30 765
Cuts at: 3782 3812 4090 4120 1178
Size: 30 278 30 4502
Fragments arranged by size:
4502 1548 765 278 165 30 30 30
30 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2739 3576 1186
Size: 1553 837 5054
Fragments arranged by size:
5054 1553 837
BseYI C'CCAG_C
Cuts at: 2010 2399 2790 2817 3681 4382 6647 7143 2010
Size: 389 391 27 864 701 2265 496 2311
Fragments arranged by size:
2311 2265 864 701 496 391 389 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2674 3474 3824 3943 4396 4469 1163
Size: 573 938 800 350 119 453 73 4138
Fragments arranged by size:
4138 938 800 573 453 350 119 73
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4894 5655 6683 1540
Size: 275 186 2893 761 1028 2301
Fragments arranged by size:
2893 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2802 3419 3525 4307 5505 5590 6661 2802
Size: 617 106 782 1198 85 1071 3585
Fragments arranged by size:
3585 1198 1071 782 617 106 85
BsmI GAATG_Cn'
Cuts at: 2739 4955 5048 2739
Size: 2216 93 5135
Fragments arranged by size:
5135 2216 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3225 3225
Size: 7444
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2206 2368 2802 2825 3419 3525 4075
Size: 399 416 162 434 23 594 106 550
Cuts at: 4075 4307 4763 5505 5590 6661 1391
Size: 232 456 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 594 550 456 434 416
399 232 162 106 85 23
BspEI T'CCGG_A
Cuts at: 7107 7107
Size: 7444
BspHI T'CATG_A
Cuts at: 3666 5333 7063 3666
Size: 1667 1730 4047
Fragments arranged by size:
4047 1730 1667
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2055 3893 4799 844
Size: 1211 1838 906 3489
Fragments arranged by size:
3489 1838 1211 906
BsrBI CCG'CTC
Cuts at: 1394 1823 5331 1394
Size: 429 3508 3507
Fragments arranged by size:
3508 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3189 3676 3970 4546 5939 6113 65
Size: 1183 1941 487 294 576 1393 174 1396
Fragments arranged by size:
1941 1396 1393 1183 576 487 294 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6088 6275 1700
Size: 110 4278 187 2869
Fragments arranged by size:
4278 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4262 4562 4730 96
Size: 4166 300 168 2810
Fragments arranged by size:
4166 2810 300 168
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7444
BssSI C'ACGA_G
Cuts at: 5504 6516 5504
Size: 1012 6432
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2672 4122 4410 794
Size: 1878 1450 288 3828
Fragments arranged by size:
3828 1878 1450 288
BstBI TT'CG_AA
Cuts at: 6248 6248
Size: 7444
BstEII G'GTnAC_C
Cuts at: 1184 4106 1184
Size: 2922 4522
BstXI CCAn_nnnn'nTGG
Cuts at: 3329 3364 3329
Size: 35 7409
BstYI r'GATC_y
Cuts at: 1972 2310 2542 3121 4008 4295 4774 5141 5526
Size: 338 232 579 887 287 479 367 385
Cuts at: 5526 5543 6995 7081 7093 7439 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 887 579 479 385 367 346
338 287 232 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3174 3357 3639 4102 513
Size: 552 939 1170 183 282 463 3855
Fragments arranged by size:
3855 1170 939 552 463 282 183
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6705
Fragments arranged by size:
6705 683 56
BtsI GCAGTG_nn'
Cuts at: 2416 2428 2686 2962 3015 3462 3931 3958 4171
Size: 12 258 276 53 447 469 27 213
Cuts at: 4171 4231 4960 5754 5774 2416
Size: 60 729 794 20 4086
Fragments arranged by size:
4086 794 729 469 447 276 258 213
60 53 27 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7444
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7409
DraI TTT'AAA
Cuts at: 4748 4748
Size: 7444
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2747 6451 809
Size: 507 1431 3704 1802
Fragments arranged by size:
3704 1802 1431 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3069 3570 3615
Size: 54 1425 50 275 18 1239 501 45
Cuts at: 3615 4033 4339 4891 5780 7409 8
Size: 418 306 552 889 1629 43
Fragments arranged by size:
1629 1425 1239 889 552 501 418 306
275 54 50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4891 1537
Size: 275 3079 4090
Fragments arranged by size:
4090 3079 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2468 3045 4489 4632 5374 1353
Size: 1115 577 1444 143 742 3423
Fragments arranged by size:
3423 1444 1115 742 577 143
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2917 3431 4664 6031 6405 6551
Size: 1064 138 1328 514 1233 1367 374 146
Cuts at: 6551 387
Size: 1280
Fragments arranged by size:
1367 1328 1280 1233 1064 514 374 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2228 2341 2676 3247 3462 3539 3741
Size: 393 596 113 335 571 215 77 202
Cuts at: 3741 4003 4470 5507 6104 6891 7159 1239
Size: 262 467 1037 597 787 268 1524
Fragments arranged by size:
1524 1037 787 597 596 571 467 393
335 268 262 215 202 113 77
EcoO109I rG'GnC_Cy
Cuts at: 2202 2203 2271 2821 3709 3905 2202
Size: 1 68 550 888 196 5741
Fragments arranged by size:
5741 888 550 196 68 1
EcoRI G'AATT_C
Cuts at: 4250 4753 5131 4250
Size: 503 378 6563
Fragments arranged by size:
6563 503 378
EcoRV GAT'ATC
Cuts at: 4522 4522
Size: 7444
FspI TGC'GCA
Cuts at: 3536 5950 3536
Size: 2414 5030
FspAI rTGC'GCAy
Cuts at: 3536 3536
Size: 7444
HaeII r_GCGC'y
Cuts at: 1678 2066 2814 4060 6591 1678
Size: 388 748 1246 2531 2531
Fragments arranged by size:
2531 2531 1246 748 388
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2521 2553 2951 2983 3044
Size: 32 135 32 949 32 398 32 61
Cuts at: 3044 3076 3716 3748 6085 6117 6159 6191 1373
Size: 32 640 32 2337 32 42 32 2626
Fragments arranged by size:
2626 2337 949 640 398 135 61 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3511 4788 5034 669
Size: 988 281 1573 1277 246 3079
Fragments arranged by size:
3079 1573 1277 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3474 748
Size: 2726 4718
HpaI GTT'AAC
Cuts at: 5034 5034
Size: 7444
KpnI G_GTAC'C
Cuts at: 3046 4769 3046
Size: 1723 5721
MfeI C'AATT_G
Cuts at: 5043 5043
Size: 7444
MluI A'CGCG_T
Cuts at: 7320 7320
Size: 7444
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2075 2338 2677 6558 6742 1305
Size: 770 263 339 3881 184 2007
Fragments arranged by size:
3881 2007 770 339 263 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3071 3572 3617 4035 10
Size: 54 1425 343 1239 501 45 418 3419
Fragments arranged by size:
3419 1425 1239 501 418 343 54 45
MslI CAynn'nnrTG
Cuts at: 518 1166 2288 2421 2756 3327 3362 3405 3800
Size: 648 1122 133 335 571 35 43 395
Cuts at: 3800 3963 5402 5761 5920 518
Size: 163 1439 359 159 2042
Fragments arranged by size:
2042 1439 1122 648 571 395 359 335
163 159 133 43 35
MspA1I CmG'CkG
Cuts at: 1606 2022 2672 2817 3087 4023 4161 4860 5537
Size: 416 650 145 270 936 138 699 677
Cuts at: 5537 6685 6930 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 936 699 677 650 416 270
245 145 138
NcoI C'CATG_G
Cuts at: 513 1065 2004 3174 3357 3639 4102 513
Size: 552 939 1170 183 282 463 3855
Fragments arranged by size:
3855 1170 939 552 463 282 183
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7444
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7444
NotI GC'GGCC_GC
Cuts at: 4891 4891
Size: 7444
NsiI A_TGCA'T
Cuts at: 4118 4118
Size: 7444
NspI r_CATG'y
Cuts at: 1132 1229 2407 3245 4120 4245 4731 4802 6347
Size: 97 1178 838 875 125 486 71 1545
Cuts at: 6347 1132
Size: 2229
Fragments arranged by size:
2229 1545 1178 875 838 486 125 97
71
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7444
PciI A'CATG_T
Cuts at: 4241 4727 6343 4241
Size: 486 1616 5342
Fragments arranged by size:
5342 1616 486
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2118 2406 1108
Size: 902 108 288 6146
Fragments arranged by size:
6146 902 288 108
PfoI T'CCnGG_A
Cuts at: 2145 2511 2145
Size: 366 7078
PmeI GTTT'AAAC
Cuts at: 4748 4748
Size: 7444
PmlI CAC'GTG
Cuts at: 2797 3403 2797
Size: 606 6838
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3589 3621 5513 5545 7026 7058 3589
Size: 32 1892 32 1481 32 3975
Fragments arranged by size:
3975 1892 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2271 3709 3905 2271
Size: 1438 196 5810
Fragments arranged by size:
5810 1438 196
PshAI GACnn'nnGTC
Cuts at: 1613 2102 2358 1613
Size: 489 256 6699
Fragments arranged by size:
6699 489 256
PsiI TTA'TAA
Cuts at: 5014 5014
Size: 7444
PspOMI G'GGCC_C
Cuts at: 2202 2821 2202
Size: 619 6825
PstI C_TGCA'G
Cuts at: 830 2423 3022 3184 4064 4293 4796 830
Size: 1593 599 162 880 229 503 3478
Fragments arranged by size:
3478 1593 880 599 503 229 162
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7444
PvuII CAG'CTG
Cuts at: 2817 4023 4161 2817
Size: 1206 138 6100
Fragments arranged by size:
6100 1206 138
SalI G'TCGA_C
Cuts at: 1936 4786 1936
Size: 2850 4594
SanDI GG'GwC_CC
Cuts at: 2271 2271
Size: 7444
SbfI CC_TGCA'GG
Cuts at: 4293 4796 4293
Size: 503 6941
ScaI AGT'ACT
Cuts at: 5692 7404 5692
Size: 1712 5732
SfcI C'TryA_G
Cuts at: 826 1045 2419 3018 3079 3117 3180 3853 4060
Size: 219 1374 599 61 38 63 673 207
Cuts at: 4060 4289 4456 4792 5927 6608 6799 826
Size: 229 167 336 1135 681 191 1471
Fragments arranged by size:
1471 1374 1135 681 673 599 336 229
219 207 191 167 63 61 38
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7444
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7444
SmaI CCC'GGG
Cuts at: 1872 2034 4771 1872
Size: 162 2737 4545
Fragments arranged by size:
4545 2737 162
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2260 5548 6449 6711 820
Size: 197 926 317 3288 901 262 1553
Fragments arranged by size:
3288 1553 926 901 317 262 197
SnaBI TAC'GTA
Cuts at: 493 5235 493
Size: 4742 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7444
SphI G_CATG'C
Cuts at: 1132 4802 1132
Size: 3670 3774
SspI AAT'ATT
Cuts at: 5 52 5368 5
Size: 47 5316 2081
Fragments arranged by size:
5316 2081 47
StuI AGG'CCT
Cuts at: 1517 2910 1517
Size: 1393 6051
StyI C'CwwG_G
Cuts at: 513 1065 2004 3038 3174 3357 3639 4102 4883
Size: 552 939 1034 136 183 282 463 781
Cuts at: 4883 513
Size: 3074
Fragments arranged by size:
3074 1034 939 781 552 463 282 183
136
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3438 3563 4389 5471 5488 5641 5826
Size: 1255 1858 125 826 1082 17 153 185
Cuts at: 5826 6271 325
Size: 445 1498
Fragments arranged by size:
1858 1498 1255 1082 826 445 185 153
125 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4244 4262 4562
Size: 274 80 33 51 1443 2267 18 300
Cuts at: 4562 4730 5690 7402 96
Size: 168 960 1712 138
Fragments arranged by size:
2267 1712 1443 960 300 274 168 138
80 51 33 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2036 2068 2635 2667 3247 3279 4090 4122 4210
Size: 32 567 32 580 32 811 32 88
Cuts at: 4210 4242 7404 7436 2036
Size: 32 3162 32 2044
Fragments arranged by size:
3162 2044 811 580 567 88 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3707 1316
Size: 2391 5053
XbaI T'CTAG_A
Cuts at: 4780 4780
Size: 7444
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2304 2304
Size: 7444
XhoI C'TCGA_G
Cuts at: 1943 2260 1943
Size: 317 7127
XmaI C'CCGG_G
Cuts at: 1870 2032 4769 1870
Size: 162 2737 4545
Fragments arranged by size:
4545 2737 162
XmnI GAAnn'nnTTC
Cuts at: 5573 5573
Size: 7444
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3159 5252 276
Size: 53 83 186 936 1625 2093 2468
Fragments arranged by size:
2468 2093 1625 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BbeI BplI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoNI FalI FseI KasI NaeI
NarI NgoMIV NruI PacI PspXI PsrI RsrII SacI
SacII SapI SexAI SfiI SfoI SrfI SwaI