Restriction map for
pFN21AB8413
(Circular) (Six-base) MAPSORT of: pFN21AB8413.seq Check: 5875 from: 1 to: 6865
pFN21AB8413 6865 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4675 278
Size: 53 83 186 936 3139 2468
Fragments arranged by size:
3139 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4208 1937
Size: 2271 4594
Acc65I G'GTAC_C
Cuts at: 4186 4186
Size: 6865
AclI AA'CG_TT
Cuts at: 1563 4992 5365 1563
Size: 3429 373 3063
Fragments arranged by size:
3429 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2558 2756 4928 6312 6580 1632
Size: 926 198 2172 1384 268 1917
Fragments arranged by size:
2172 1917 1384 926 268 198
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6668
AflIII A'CryG_T
Cuts at: 1203 2562 4011 5764 6741 1203
Size: 1359 1449 1753 977 1327
Fragments arranged by size:
1753 1449 1359 1327 977
AgeI A'CCGG_T
Cuts at: 5696 5696
Size: 6865
AhdI GACnn_n'nnGTC
Cuts at: 2983 3568 5594 2983
Size: 585 2026 4254
Fragments arranged by size:
4254 2026 585
AleI CACnn'nnGTG
Cuts at: 1166 2433 3747 1166
Size: 1267 1314 4284
Fragments arranged by size:
4284 1314 1267
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2402 1184
Size: 1218 5647
AlwNI CAG_nnn'CTG
Cuts at: 2623 2987 3640 4608 6180 2623
Size: 364 653 968 1572 3308
Fragments arranged by size:
3308 1572 968 653 364
ApaLI G'TGCA_C
Cuts at: 4922 6078 4922
Size: 1156 5709
ApoI r'AATT_y
Cuts at: 1493 3804 4174 4400 4552 6661 1493
Size: 2311 370 226 152 2109 1697
Fragments arranged by size:
2311 2109 1697 370 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2721 5419 160
Size: 872 1689 2698 1606
Fragments arranged by size:
2698 1689 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6865
AvaI C'yCGr_G
Cuts at: 1870 1943 2250 3450 3608 4190 1870
Size: 73 307 1200 158 582 4545
Fragments arranged by size:
4545 1200 582 307 158 73
BamHI G'GATC_C
Cuts at: 4195 4562 4195
Size: 367 6498
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4186 5641 618
Size: 325 217 627 2399 1455 1842
Fragments arranged by size:
2399 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2475 3243 3348 4134 4184 1391
Size: 1084 768 105 786 50 4072
Fragments arranged by size:
4072 1084 786 768 105 50
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3207 928
Size: 971 1308 4586
Fragments arranged by size:
4586 1308 971
BbvCI CC'TCA_GC
Cuts at: 2175 3590 2175
Size: 1415 5450
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3431 3465 5056 5090 1903
Size: 34 50 34 1410 34 1591 34 3678
Fragments arranged by size:
3678 1591 1410 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4757 5967 4757
Size: 1210 5655
BclI T'GATC_A
Cuts at: 1548 3624 1548
Size: 2076 4789
BfrBI ATG'CAT
Cuts at: 2774 2774
Size: 6865
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3222 5476 136
Size: 107 122 71 631 605 1550 2254 1525
Fragments arranged by size:
2254 1550 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6860 6860
Size: 6865
BlpI GC'TnA_GC
Cuts at: 4052 4282 4052
Size: 230 6635
Bme1580I G_kGCm'C
Cuts at: 1790 3997 4926 6082 1790
Size: 2207 929 1156 2573
Fragments arranged by size:
2573 2207 1156 929
BmgBI CAC'GTC
Cuts at: 1318 2344 2563 1318
Size: 1026 219 5620
Fragments arranged by size:
5620 1026 219
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2275 3846 4596
Size: 416 53 83 399 24 858 1571 750
Cuts at: 4596 5554 442
Size: 958 1753
Fragments arranged by size:
1753 1571 958 858 750 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6865
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2615 2660 4106 5525 1239
Size: 1376 45 1446 1419 2579
Fragments arranged by size:
2579 1446 1419 1376 45
Bpu10I CC'TnA_GC
Cuts at: 2175 3590 2175
Size: 1415 5450
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3638 4990 5855 6153 3638
Size: 1352 865 298 4350
Fragments arranged by size:
4350 1352 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2291 2686 3926 5528 882
Size: 617 792 395 1240 1602 2219
Fragments arranged by size:
2219 1602 1240 792 617 395
BsaAI yAC'GTr
Cuts at: 493 1204 4656 493
Size: 711 3452 2702
Fragments arranged by size:
3452 2702 711
BsaBI GATnn'nnATC
Cuts at: 6859 6859
Size: 6865
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4672 5054 5689 275
Size: 53 83 186 936 3139 382 635 1451
Fragments arranged by size:
3139 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2793 3171 5298 5696 5970 6117 6528 2793
Size: 378 2127 398 274 147 411 3130
Fragments arranged by size:
3130 2127 411 398 378 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3088 3118 1178
Size: 30 165 30 1685 30 4925
Fragments arranged by size:
4925 1685 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2977 3096 1186
Size: 1791 119 4955
Fragments arranged by size:
4955 1791 119
BseYI C'CCAG_C
Cuts at: 2061 3058 4133 6068 6564 2061
Size: 997 1075 1935 496 2362
Fragments arranged by size:
2362 1935 1075 997 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2241 1163
Size: 573 505 5787
Fragments arranged by size:
5787 573 505
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4315 5076 6104 1540
Size: 275 186 2314 761 1028 2301
Fragments arranged by size:
2314 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2475 3243 3348 3874 4926 5011 6082 2475
Size: 768 105 526 1052 85 1071 3258
Fragments arranged by size:
3258 1071 1052 768 526 105 85
BsmI GAATG_Cn'
Cuts at: 2447 3120 3218 4376 4469 2447
Size: 673 98 1158 93 4843
Fragments arranged by size:
4843 1158 673 98 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2475 3243 3348 3874 3997 4134 4184
Size: 399 685 768 105 526 123 137 50
Cuts at: 4184 4926 5011 6082 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 768 742 685 526 399 137
123 105 85 50
BspEI T'CCGG_A
Cuts at: 3171 6528 3171
Size: 3357 3508
BspHI T'CATG_A
Cuts at: 4754 6484 4754
Size: 1730 5135
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3259 4220 844
Size: 2415 961 3489
Fragments arranged by size:
3489 2415 961
BsrBI CCG'CTC
Cuts at: 1394 1823 4752 1394
Size: 429 2929 3507
Fragments arranged by size:
3507 2929 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2709 3004 3304 5360 5534 65
Size: 1183 1461 295 300 2056 174 1396
Fragments arranged by size:
2056 1461 1396 1183 300 295 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3645 3714 5509 5696 1700
Size: 110 1835 69 1795 187 2869
Fragments arranged by size:
2869 1835 1795 187 110 69
BsrGI T'GTAC_A
Cuts at: 96 2181 3036 96
Size: 2085 855 3925
Fragments arranged by size:
3925 2085 855
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6865
BssSI C'ACGA_G
Cuts at: 2399 4925 5937 2399
Size: 2526 1012 3327
Fragments arranged by size:
3327 2526 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6865
BstBI TT'CG_AA
Cuts at: 5669 5669
Size: 6865
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6865
BstXI CCAn_nnnn'nTGG
Cuts at: 3478 3478
Size: 6865
BstYI r'GATC_y
Cuts at: 1972 3600 3612 4195 4562 4947 4964 6416 6502
Size: 1628 12 583 367 385 17 1452 86
Cuts at: 6502 6514 6860 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1628 1452 583 385 367 346 86
17 12 12
Bsu36I CC'TnA_GG
Cuts at: 2745 2745
Size: 6865
BtgI C'CryG_G
Cuts at: 513 1065 2482 2553 2652 3087 513
Size: 552 1417 71 99 435 4291
Fragments arranged by size:
4291 1417 552 435 99 71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3931 487
Size: 683 56 2705 3421
Fragments arranged by size:
3421 2705 683 56
BtsI GCAGTG_nn'
Cuts at: 2209 3235 4381 5175 5195 2209
Size: 1026 1146 794 20 3879
Fragments arranged by size:
3879 1146 1026 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6865
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2382 2417 529
Size: 35 1818 35 4977
Fragments arranged by size:
4977 1818 35 35
DraI TTT'AAA
Cuts at: 4169 4169
Size: 6865
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5872 809
Size: 507 4556 1802
Fragments arranged by size:
4556 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2045 2325
Size: 54 1425 50 275 18 204 11 280
Cuts at: 2325 2586 3223 4312 5201 6830 8
Size: 261 637 1089 889 1629 43
Fragments arranged by size:
1629 1425 1089 889 637 280 275 261
204 54 50 43 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 4312 1537
Size: 275 2500 4090
Fragments arranged by size:
4090 2500 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3336 4795 1353
Size: 1983 1459 3423
Fragments arranged by size:
3423 1983 1459
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5452 5826 5972 387
Size: 1064 138 3863 374 146 1280
Fragments arranged by size:
3863 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2473 3241 3346 2473
Size: 768 105 5992
Fragments arranged by size:
5992 768 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2558 2615 2660 2756 4106 4928 5525
Size: 393 926 57 45 96 1350 822 597
Cuts at: 5525 6312 6580 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1350 926 822 787 597 393 268
96 57 45
EcoO109I rG'GnC_Cy
Cuts at: 2277 2580 3736 2277
Size: 303 1156 5406
Fragments arranged by size:
5406 1156 303
EcoRI G'AATT_C
Cuts at: 4174 4552 4174
Size: 378 6487
FspI TGC'GCA
Cuts at: 5371 5371
Size: 6865
HaeII r_GCGC'y
Cuts at: 1678 3640 6012 1678
Size: 1962 2372 2531
Fragments arranged by size:
2531 2372 1962
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2111 2143 2649 2681 2839
Size: 32 135 32 539 32 506 32 158
Cuts at: 2839 2871 2969 3001 3429 3461 3538 3554 3570
Size: 32 98 32 428 32 77 16 16
Cuts at: 3570 3586 5506 5538 5580 5612 1373
Size: 16 1920 32 42 32 2626
Fragments arranged by size:
2626 1920 539 506 428 158 135 98
77 42 32 32 32 32 32 32
32 32 32 16 16 16
HincII GTy'rAC
Cuts at: 669 1657 1938 4209 4455 669
Size: 988 281 2271 246 3079
Fragments arranged by size:
3079 2271 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6865
HpaI GTT'AAC
Cuts at: 4455 4455
Size: 6865
KpnI G_GTAC'C
Cuts at: 4190 4190
Size: 6865
MfeI C'AATT_G
Cuts at: 3579 4464 3579
Size: 885 5980
MluI A'CGCG_T
Cuts at: 6741 6741
Size: 6865
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2313 2709 3876 5979 6163 1305
Size: 1008 396 1167 2103 184 2007
Fragments arranged by size:
2103 2007 1167 1008 396 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2036 2047 2327 2588 10
Size: 54 1425 343 204 11 280 261 4287
Fragments arranged by size:
4287 1425 343 280 261 204 54 11
MslI CAynn'nnrTG
Cuts at: 518 1166 2369 2384 2433 3248 3747 4061 4823
Size: 648 1203 15 49 815 499 314 762
Cuts at: 4823 5182 5341 518
Size: 359 159 2042
Fragments arranged by size:
2042 1203 815 762 648 499 359 314
159 49 15
MspA1I CmG'CkG
Cuts at: 1606 2179 2266 3367 4281 4958 6106 6351 1606
Size: 573 87 1101 914 677 1148 245 2120
Fragments arranged by size:
2120 1148 1101 914 677 573 245 87
NcoI C'CATG_G
Cuts at: 513 1065 2482 2553 3087 513
Size: 552 1417 71 534 4291
Fragments arranged by size:
4291 1417 552 534 71
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6865
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6865
NotI GC'GGCC_GC
Cuts at: 4312 4312
Size: 6865
NsiI A_TGCA'T
Cuts at: 2776 2776
Size: 6865
NspI r_CATG'y
Cuts at: 1132 1229 3136 4015 4223 5768 1132
Size: 97 1907 879 208 1545 2229
Fragments arranged by size:
2229 1907 1545 879 208 97
PasI CC'CwG_GG
Cuts at: 1348 3005 1348
Size: 1657 5208
PciI A'CATG_T
Cuts at: 4011 5764 4011
Size: 1753 5112
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2043 2269 2554 1108
Size: 935 226 285 5419
Fragments arranged by size:
5419 935 285 226
PfoI T'CCnGG_A
Cuts at: 2592 3109 4077 2592
Size: 517 968 5380
Fragments arranged by size:
5380 968 517
PmeI GTTT'AAAC
Cuts at: 4169 4169
Size: 6865
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4934 4966 6447 6479 4934
Size: 32 1481 32 5320
Fragments arranged by size:
5320 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2277 2580 3736 2277
Size: 303 1156 5406
Fragments arranged by size:
5406 1156 303
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6865
PsiI TTA'TAA
Cuts at: 4435 4435
Size: 6865
PspXI vC'TCGA_Gb
Cuts at: 3450 3450
Size: 6865
PstI C_TGCA'G
Cuts at: 830 3270 3798 4217 830
Size: 2440 528 419 3478
Fragments arranged by size:
3478 2440 528 419
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6865
PvuII CAG'CTG
Cuts at: 2179 2266 3367 2179
Size: 87 1101 5677
Fragments arranged by size:
5677 1101 87
SacI G_AGCT'C
Cuts at: 2475 3243 3348 2475
Size: 768 105 5992
Fragments arranged by size:
5992 768 105
SalI G'TCGA_C
Cuts at: 1936 4207 1936
Size: 2271 4594
SapI GCTCTTCn'nnn_
Cuts at: 3336 3336
Size: 6865
SbfI CC_TGCA'GG
Cuts at: 3270 3798 4217 3270
Size: 528 419 5918
Fragments arranged by size:
5918 528 419
ScaI AGT'ACT
Cuts at: 3968 5113 6825 3968
Size: 1145 1712 4008
Fragments arranged by size:
4008 1712 1145
SfcI C'TryA_G
Cuts at: 826 1045 2431 3266 3794 4213 5348 6029 6220
Size: 219 1386 835 528 419 1135 681 191
Cuts at: 6220 826
Size: 1471
Fragments arranged by size:
1471 1386 1135 835 681 528 419 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6865
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6865
SmaI CCC'GGG
Cuts at: 1872 2252 4192 1872
Size: 380 1940 4545
Fragments arranged by size:
4545 1940 380
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3450 3617 4969 5870 6132 820
Size: 197 926 1507 167 1352 901 262 1553
Fragments arranged by size:
1553 1507 1352 926 901 262 197 167
SnaBI TAC'GTA
Cuts at: 493 4656 493
Size: 4163 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6865
SphI G_CATG'C
Cuts at: 1132 4223 1132
Size: 3091 3774
SspI AAT'ATT
Cuts at: 5 52 4789 5
Size: 47 4737 2081
Fragments arranged by size:
4737 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6865
StyI C'CwwG_G
Cuts at: 513 1065 2482 2553 3087 3123 4125 4304 513
Size: 552 1417 71 534 36 1002 179 3074
Fragments arranged by size:
3074 1417 1002 552 534 179 71 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2112 3032 3345 4892 4909 5062 5247
Size: 1255 532 920 313 1547 17 153 185
Cuts at: 5247 5692 325
Size: 445 1498
Fragments arranged by size:
1547 1498 1255 920 532 445 313 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 3036 3138
Size: 274 80 33 51 1443 204 855 102
Cuts at: 3138 3966 5111 6823 96
Size: 828 1145 1712 138
Fragments arranged by size:
1712 1443 1145 855 828 274 204 138
102 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4104 4136 6825 6857 4104
Size: 32 2689 32 4112
Fragments arranged by size:
4112 2689 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2024 2281 4148 1316
Size: 708 257 1867 4033
Fragments arranged by size:
4033 1867 708 257
XbaI T'CTAG_A
Cuts at: 4201 4201
Size: 6865
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2320 3107 4036 2320
Size: 787 929 5149
Fragments arranged by size:
5149 929 787
XhoI C'TCGA_G
Cuts at: 1943 3450 1943
Size: 1507 5358
XmaI C'CCGG_G
Cuts at: 1870 2250 4190 1870
Size: 380 1940 4545
Fragments arranged by size:
4545 1940 380
XmnI GAAnn'nnTTC
Cuts at: 4994 4994
Size: 6865
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4673 276
Size: 53 83 186 936 3139 2468
Fragments arranged by size:
3139 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI