Restriction map for
pFN21AB8403
(Circular) (Six-base) MAPSORT of: pFN21AB8403.seq Check: 1511 from: 1 to: 6850
pFN21AB8403 6850 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2508 4660 278
Size: 53 83 186 936 972 2152 2468
Fragments arranged by size:
2468 2152 972 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2475 4193 1937
Size: 538 1718 4594
Fragments arranged by size:
4594 1718 538
Acc65I G'GTAC_C
Cuts at: 4171 4171
Size: 6850
AclI AA'CG_TT
Cuts at: 1563 4977 5350 1563
Size: 3414 373 3063
Fragments arranged by size:
3414 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2964 3522 4913 6297 6565 1632
Size: 1332 558 1391 1384 268 1917
Fragments arranged by size:
1917 1391 1384 1332 558 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6653
AflIII A'CryG_T
Cuts at: 1203 2098 3103 3771 5749 6726 1203
Size: 895 1005 668 1978 977 1327
Fragments arranged by size:
1978 1327 1005 977 895 668
AgeI A'CCGG_T
Cuts at: 5681 5681
Size: 6850
AhdI GACnn_n'nnGTC
Cuts at: 5579 5579
Size: 6850
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6850
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6850
AlwNI CAG_nnn'CTG
Cuts at: 2431 4019 4593 6165 2431
Size: 1588 574 1572 3116
Fragments arranged by size:
3116 1588 1572 574
ApaLI G'TGCA_C
Cuts at: 4907 6063 4907
Size: 1156 5694
ApoI r'AATT_y
Cuts at: 1493 2348 2386 2805 2826 3451 3479 3581 3871
Size: 855 38 419 21 625 28 102 290
Cuts at: 3871 4159 4385 4537 6646 1493
Size: 288 226 152 2109 1697
Fragments arranged by size:
2109 1697 855 625 419 290 288 226
152 102 38 28 21
AseI AT'TA_AT
Cuts at: 160 1032 3035 3474 5404 160
Size: 872 2003 439 1930 1606
Fragments arranged by size:
2003 1930 1606 872 439
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6850
AvaI C'yCGr_G
Cuts at: 1870 1943 4175 1870
Size: 73 2232 4545
Fragments arranged by size:
4545 2232 73
BamHI G'GATC_C
Cuts at: 4180 4547 4180
Size: 367 6483
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4171 5626 618
Size: 325 217 627 2384 1455 1842
Fragments arranged by size:
2384 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2583 4169 1391
Size: 1192 1586 4072
Fragments arranged by size:
4072 1586 1192
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2301 928
Size: 971 402 5477
Fragments arranged by size:
5477 971 402
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2019 2053 3053 3087 5041 5075 1903
Size: 34 82 34 1000 34 1954 34 3678
Fragments arranged by size:
3678 1954 1000 82 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2855 3721 4742 5952 2855
Size: 866 1021 1210 3753
Fragments arranged by size:
3753 1210 1021 866
BclI T'GATC_A
Cuts at: 1548 2053 1548
Size: 505 6345
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5461 136
Size: 107 122 71 631 605 3789 1525
Fragments arranged by size:
3789 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6845 6845
Size: 6850
BlpI GC'TnA_GC
Cuts at: 2838 4267 2838
Size: 1429 5421
Bme1580I G_kGCm'C
Cuts at: 1790 3092 4911 6067 1790
Size: 1302 1819 1156 2573
Fragments arranged by size:
2573 1819 1302 1156
BmgBI CAC'GTC
Cuts at: 1318 2020 1318
Size: 702 6148
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2771 4581 5539
Size: 416 53 83 399 24 1354 1810 958
Cuts at: 5539 442
Size: 1753
Fragments arranged by size:
1810 1753 1354 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6850
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2890 2890
Size: 6850
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2616 3919 5510 1239
Size: 1377 1303 1591 2579
Fragments arranged by size:
2579 1591 1377 1303
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3837 4044 4975 5840 6138 3837
Size: 207 931 865 298 4549
Fragments arranged by size:
4549 931 865 298 207
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5513 882
Size: 617 4014 2219
Fragments arranged by size:
4014 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4641 493
Size: 711 3437 2702
Fragments arranged by size:
3437 2702 711
BsaBI GATnn'nnATC
Cuts at: 6844 6844
Size: 6850
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2505 4657 5039 5674
Size: 53 83 186 936 972 2152 382 635
Cuts at: 5674 275
Size: 1451
Fragments arranged by size:
2152 1451 972 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5283 5681 5955 6102 6513 5283
Size: 398 274 147 411 5620
Fragments arranged by size:
5620 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6625
Fragments arranged by size:
6625 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3642 1186
Size: 2456 4394
BseYI C'CCAG_C
Cuts at: 6053 6549 6053
Size: 496 6354
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6277
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4300 5061 6089 1540
Size: 275 186 2299 761 1028 2301
Fragments arranged by size:
2301 2299 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4911 4996 6067 4911
Size: 85 1071 5694
Fragments arranged by size:
5694 1071 85
BsmI GAATG_Cn'
Cuts at: 4361 4454 4361
Size: 93 6757
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2583 3092 4169 4911 4996 6067 1391
Size: 399 793 509 1077 742 85 1071 2174
Fragments arranged by size:
2174 1077 1071 793 742 509 399 85
BspEI T'CCGG_A
Cuts at: 6513 6513
Size: 6850
BspHI T'CATG_A
Cuts at: 2050 3985 4739 6469 2050
Size: 1935 754 1730 2431
Fragments arranged by size:
2431 1935 1730 754
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2207 2505 4205 844
Size: 1363 298 1700 3489
Fragments arranged by size:
3489 1700 1363 298
BsrBI CCG'CTC
Cuts at: 1394 1823 4737 1394
Size: 429 2914 3507
Fragments arranged by size:
3507 2914 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5345 5519 65
Size: 1183 4097 174 1396
Fragments arranged by size:
4097 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5494 5681 1700
Size: 110 3684 187 2869
Fragments arranged by size:
3684 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2936 96
Size: 2840 4010
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6850
BssSI C'ACGA_G
Cuts at: 4910 5922 4910
Size: 1012 5838
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6850
BstBI TT'CG_AA
Cuts at: 3178 5654 3178
Size: 2476 4374
BstEII G'GTnAC_C
Cuts at: 1184 2194 1184
Size: 1010 5840
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6850
BstYI r'GATC_y
Cuts at: 1972 2104 2395 2531 4180 4547 4932 4949 6401
Size: 132 291 136 1649 367 385 17 1452
Cuts at: 6401 6487 6499 6845 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1649 1452 385 367 346 291 136
132 86 17 12
BstZ17I GTA'TAC
Cuts at: 2476 2476
Size: 6850
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5359
Fragments arranged by size:
5359 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6111
Fragments arranged by size:
6111 683 56
BtsI GCAGTG_nn'
Cuts at: 4366 5160 5180 4366
Size: 794 20 6036
Fragments arranged by size:
6036 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6850
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6815
DraI TTT'AAA
Cuts at: 2644 2929 2991 3334 4154 2644
Size: 285 62 343 820 5340
Fragments arranged by size:
5340 820 343 285 62
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3518 5857 809
Size: 507 2202 2339 1802
Fragments arranged by size:
2339 2202 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4297 5186 6815
Size: 54 1425 50 275 18 2467 889 1629
Cuts at: 6815 8
Size: 43
Fragments arranged by size:
2467 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4297 1537
Size: 275 2485 4090
Fragments arranged by size:
4090 2485 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2411 4109 4780 1353
Size: 1058 1698 671 3423
Fragments arranged by size:
3423 1698 1058 671
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2030 5437 5811 5957 387
Size: 1064 138 441 3407 374 146 1280
Fragments arranged by size:
3407 1280 1064 441 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2616 2964 3522 3919 4913 5510 6297
Size: 393 984 348 558 397 994 597 787
Cuts at: 6297 6565 1239
Size: 268 1524
Fragments arranged by size:
1524 994 984 787 597 558 397 393
348 268
EcoO109I rG'GnC_Cy
Cuts at: 3839 3839
Size: 6850
EcoRI G'AATT_C
Cuts at: 3479 4159 4537 3479
Size: 680 378 5792
Fragments arranged by size:
5792 680 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2993 3575 2993
Size: 582 6268
FspI TGC'GCA
Cuts at: 5356 5356
Size: 6850
HaeII r_GCGC'y
Cuts at: 1678 5997 1678
Size: 4319 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2128 2160 3024 3056 3677
Size: 32 135 32 556 32 864 32 621
Cuts at: 3677 3709 5491 5523 5565 5597 1373
Size: 32 1782 32 42 32 2626
Fragments arranged by size:
2626 1782 864 621 556 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2684 3511 4194 4440 669
Size: 988 281 746 827 683 246 3079
Fragments arranged by size:
3079 988 827 746 683 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6850
HpaI GTT'AAC
Cuts at: 4440 4440
Size: 6850
KpnI G_GTAC'C
Cuts at: 4175 4175
Size: 6850
MfeI C'AATT_G
Cuts at: 4449 4449
Size: 6850
MluI A'CGCG_T
Cuts at: 6726 6726
Size: 6850
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2509 5964 6148 1305
Size: 1204 3455 184 2007
Fragments arranged by size:
3455 2007 1204 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5028
Fragments arranged by size:
5028 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3350 4808 5167 5326 518
Size: 648 2184 1458 359 159 2042
Fragments arranged by size:
2184 2042 1458 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2431 4266 4943 6091 6336 1606
Size: 825 1835 677 1148 245 2120
Fragments arranged by size:
2120 1835 1148 825 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5359
Fragments arranged by size:
5359 939 552
NdeI CA'TA_TG
Cuts at: 387 3351 387
Size: 2964 3886
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6850
NotI GC'GGCC_GC
Cuts at: 4297 4297
Size: 6850
NspI r_CATG'y
Cuts at: 1132 1229 2102 3107 3620 3775 4208 5753 1132
Size: 97 873 1005 513 155 433 1545 2229
Fragments arranged by size:
2229 1545 1005 873 513 433 155 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6850
PciI A'CATG_T
Cuts at: 2098 3103 3771 5749 2098
Size: 1005 668 1978 3199
Fragments arranged by size:
3199 1978 1005 668
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6850
PmeI GTTT'AAAC
Cuts at: 4154 4154
Size: 6850
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4919 4951 6432 6464 4919
Size: 32 1481 32 5305
Fragments arranged by size:
5305 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3839 3839
Size: 6850
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6850
PsiI TTA'TAA
Cuts at: 2325 4420 2325
Size: 2095 4755
PstI C_TGCA'G
Cuts at: 830 4202 830
Size: 3372 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6850
PvuII CAG'CTG
Cuts at: 2431 2431
Size: 6850
SalI G'TCGA_C
Cuts at: 1936 4192 1936
Size: 2256 4594
SbfI CC_TGCA'GG
Cuts at: 4202 4202
Size: 6850
ScaI AGT'ACT
Cuts at: 5098 6810 5098
Size: 1712 5138
SexAI A'CCwGG_T
Cuts at: 2722 2722
Size: 6850
SfcI C'TryA_G
Cuts at: 826 1045 2308 2423 3948 4198 5333 6014 6205
Size: 219 1263 115 1525 250 1135 681 191
Cuts at: 6205 826
Size: 1471
Fragments arranged by size:
1525 1471 1263 1135 681 250 219 191
115
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6850
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6850
SmaI CCC'GGG
Cuts at: 1872 4177 1872
Size: 2305 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3852 4059 4954 5855 6117 820
Size: 197 926 1909 207 895 901 262 1553
Fragments arranged by size:
1909 1553 926 901 895 262 207 197
SnaBI TAC'GTA
Cuts at: 493 4641 493
Size: 4148 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6850
SphI G_CATG'C
Cuts at: 1132 4208 1132
Size: 3076 3774
SspI AAT'ATT
Cuts at: 5 52 2186 4774 5
Size: 47 2134 2588 2081
Fragments arranged by size:
2588 2134 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6850
StyI C'CwwG_G
Cuts at: 513 1065 2004 4289 513
Size: 552 939 2285 3074
Fragments arranged by size:
3074 2285 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2815 4877 4894 5047 5232 5677 325
Size: 1255 1235 2062 17 153 185 445 1498
Fragments arranged by size:
2062 1498 1255 1235 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2095 2936 3416
Size: 274 80 33 51 1443 118 841 480
Cuts at: 3416 4087 5096 6808 96
Size: 671 1009 1712 138
Fragments arranged by size:
1712 1443 1009 841 671 480 274 138
118 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3583 3615 6810 6842 3583
Size: 32 3195 32 3591
Fragments arranged by size:
3591 3195 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2461 1316
Size: 1145 5705
XbaI T'CTAG_A
Cuts at: 3623 4186 3623
Size: 563 6287
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6850
XmaI C'CCGG_G
Cuts at: 1870 4175 1870
Size: 2305 4545
XmnI GAAnn'nnTTC
Cuts at: 3360 3582 4979 3360
Size: 222 1397 5231
Fragments arranged by size:
5231 1397 222
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2506 4658 276
Size: 53 83 186 936 972 2152 2468
Fragments arranged by size:
2468 2152 972 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI