Restriction map for
pFN21AB8402
(Circular) (Six-base) MAPSORT of: pFN21AB8402.seq Check: 5332 from: 1 to: 7051
pFN21AB8402 7051 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2541 4861 278
Size: 53 83 186 936 1005 2320 2468
Fragments arranged by size:
2468 2320 1005 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4394 1937
Size: 2457 4594
Acc65I G'GTAC_C
Cuts at: 4372 4372
Size: 7051
AclI AA'CG_TT
Cuts at: 1563 5178 5551 1563
Size: 3615 373 3063
Fragments arranged by size:
3615 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2191 2377 3644 3668 3680 3836 4085 5114
Size: 559 186 1267 24 12 156 249 1029
Cuts at: 5114 6498 6766 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1267 1029 559 268 249 186
156 24 12
AfeI AGC'GCT
Cuts at: 2915 3134 3218 3638 3890 2915
Size: 219 84 420 252 6076
Fragments arranged by size:
6076 420 252 219 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6854
AflIII A'CryG_T
Cuts at: 1203 2645 5950 6927 1203
Size: 1442 3305 977 1327
Fragments arranged by size:
3305 1442 1327 977
AgeI A'CCGG_T
Cuts at: 5882 5882
Size: 7051
AhdI GACnn_n'nnGTC
Cuts at: 5780 5780
Size: 7051
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7051
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7051
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3260 3292 3512 3544 3680 3712 3260
Size: 32 220 32 136 32 6599
Fragments arranged by size:
6599 220 136 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3165 3409 3424 3622 3844 3928 4093 4794 6366
Size: 244 15 198 222 84 165 701 1572
Cuts at: 6366 3165
Size: 3850
Fragments arranged by size:
3850 1572 701 244 222 198 165 84
15
ApaI G_GGCC'C
Cuts at: 2804 3108 2804
Size: 304 6747
ApaLI G'TGCA_C
Cuts at: 2896 3011 3593 3761 5108 6264 2896
Size: 115 582 168 1347 1156 3683
Fragments arranged by size:
3683 1347 1156 582 168 115
ApoI r'AATT_y
Cuts at: 1493 2320 2663 4360 4586 4738 6847 1493
Size: 827 343 1697 226 152 2109 1697
Fragments arranged by size:
2109 1697 1697 827 343 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5605 160
Size: 872 4573 1606
Fragments arranged by size:
4573 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7051
AvaI C'yCGr_G
Cuts at: 1870 1943 2417 2706 3016 4187 4376 1870
Size: 73 474 289 310 1171 189 4545
Fragments arranged by size:
4545 1171 474 310 289 189 73
BamHI G'GATC_C
Cuts at: 2378 4381 4748 2378
Size: 2003 367 4681
Fragments arranged by size:
4681 2003 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2949 3414 4167 4372 5827
Size: 325 217 627 1162 465 753 205 1455
Cuts at: 5827 618
Size: 1842
Fragments arranged by size:
1842 1455 1162 753 627 465 325 217
205
BanII G_rGCy'C
Cuts at: 1391 2205 2426 2449 2804 3108 3499 4370 1391
Size: 814 221 23 355 304 391 871 4072
Fragments arranged by size:
4072 871 814 391 355 304 221 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6080
BbvCI CC'TCA_GC
Cuts at: 4163 4163
Size: 7051
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2398 2432 3286 3320 5242 5276 1903
Size: 34 461 34 854 34 1922 34 3678
Fragments arranged by size:
3678 1922 854 461 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4943 6153 4943
Size: 1210 5841
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7051
BfrBI ATG'CAT
Cuts at: 2266 2266
Size: 7051
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2020 3651 4089
Size: 107 122 71 631 605 348 1631 438
Cuts at: 4089 4192 5662 136
Size: 103 1470 1525
Fragments arranged by size:
1631 1525 1470 631 605 438 348 122
107 103 71
BglII A'GATC_T
Cuts at: 7046 7046
Size: 7051
BlpI GC'TnA_GC
Cuts at: 3467 3830 4079 4313 4468 3467
Size: 363 249 234 155 6050
Fragments arranged by size:
6050 363 249 234 155
Bme1580I G_kGCm'C
Cuts at: 1790 2804 2900 3015 3108 3455 3597 3765 3791
Size: 1014 96 115 93 347 142 168 26
Cuts at: 3791 5112 6268 1790
Size: 1321 1156 2573
Fragments arranged by size:
2573 1321 1156 1014 347 168 142 115
96 93 26
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7051
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2466 2887 2918
Size: 416 53 83 399 24 1049 421 31
Cuts at: 2918 4782 5740 442
Size: 1864 958 1753
Fragments arranged by size:
1864 1753 1049 958 421 416 399 83
53 31 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7051
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2721 2721
Size: 7051
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2986 3201 3500 5711 1239
Size: 1747 215 299 2211 2579
Fragments arranged by size:
2579 2211 1747 299 215
Bpu10I CC'TnA_GC
Cuts at: 4163 4163
Size: 7051
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5176 6041 6339 5176
Size: 865 298 5888
Fragments arranged by size:
5888 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3265 3769 5714 882
Size: 617 1766 504 1945 2219
Fragments arranged by size:
2219 1945 1766 617 504
BsaAI yAC'GTr
Cuts at: 493 1204 2648 4842 493
Size: 711 1444 2194 2702
Fragments arranged by size:
2702 2194 1444 711
BsaBI GATnn'nnATC
Cuts at: 7045 7045
Size: 7051
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2439 2538 4858 5240
Size: 53 83 186 936 906 99 2320 382
Cuts at: 5240 5875 275
Size: 635 1451
Fragments arranged by size:
2320 1451 936 906 635 382 186 99
83 53
BsaWI w'CCGG_w
Cuts at: 2640 2724 2808 3363 3874 5484 5882 6156 6303
Size: 84 84 555 511 1610 398 274 147
Cuts at: 6303 6714 2640
Size: 411 2977
Fragments arranged by size:
2977 1610 555 511 411 398 274 147
84 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3260 3290 3512 3542 3680
Size: 30 165 30 1857 30 222 30 138
Cuts at: 3680 3710 1178
Size: 30 4519
Fragments arranged by size:
4519 1857 222 165 138 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2242 2439 1186
Size: 1056 197 5798
Fragments arranged by size:
5798 1056 197
BseYI C'CCAG_C
Cuts at: 2559 2855 6254 6750 2559
Size: 296 3399 496 2860
Fragments arranged by size:
3399 2860 496 296
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3410 3830 3914 4079 1163
Size: 573 1674 420 84 165 4135
Fragments arranged by size:
4135 1674 573 420 165 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4501 5262 6290 1540
Size: 275 186 2500 761 1028 2301
Fragments arranged by size:
2500 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2558 2900 3015 3333 3585 3597 3753 3765 4002
Size: 342 115 318 252 12 156 12 237
Cuts at: 4002 4254 5112 5197 6268 2558
Size: 252 858 85 1071 3341
Fragments arranged by size:
3341 1071 858 342 318 252 252 237
156 115 85 12 12
BsmI GAATG_Cn'
Cuts at: 4562 4655 4562
Size: 93 6958
BsmBI CGTCTCn'nnnn_
Cuts at: 2146 2675 2146
Size: 529 6522
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2205 2426 2449 2558 2804 2900 3015
Size: 399 415 221 23 109 246 96 115
Cuts at: 3015 3108 3333 3455 3499 3585 3597 3753 3765
Size: 93 225 122 44 86 12 156 12
Cuts at: 3765 3791 4002 4254 4370 5112 5197 6268 1391
Size: 26 211 252 116 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 415 399 252 246 225
221 211 156 122 116 115 109 96
93 86 85 44 26 23 12 12
BspEI T'CCGG_A
Cuts at: 2808 3874 6714 2808
Size: 1066 2840 3145
Fragments arranged by size:
3145 2840 1066
BspHI T'CATG_A
Cuts at: 4940 6670 4940
Size: 1730 5321
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3232 4406 844
Size: 2388 1174 3489
Fragments arranged by size:
3489 2388 1174
BsrBI CCG'CTC
Cuts at: 1394 1823 4938 1394
Size: 429 3115 3507
Fragments arranged by size:
3507 3115 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3205 4335 5546 5720 65
Size: 1183 1957 1130 1211 174 1396
Fragments arranged by size:
1957 1396 1211 1183 1130 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2946 2971 3501 5695 5882 1700
Size: 110 1136 25 530 2194 187 2869
Fragments arranged by size:
2869 2194 1136 530 187 110 25
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7051
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7051
BssSI C'ACGA_G
Cuts at: 2840 3700 5111 6123 2840
Size: 860 1411 1012 3768
Fragments arranged by size:
3768 1411 1012 860
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2904 794
Size: 2110 4941
BstBI TT'CG_AA
Cuts at: 5855 5855
Size: 7051
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7051
BstXI CCAn_nnnn'nTGG
Cuts at: 2515 2515
Size: 7051
BstYI r'GATC_y
Cuts at: 1972 2077 2353 2378 2518 4381 4748 5133 5150
Size: 105 276 25 140 1863 367 385 17
Cuts at: 5150 6602 6688 6700 7046 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1863 1452 385 367 346 276 140
105 86 25 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2693 2992 513
Size: 552 939 689 299 4572
Fragments arranged by size:
4572 939 689 552 299
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6312
Fragments arranged by size:
6312 683 56
BtsI GCAGTG_nn'
Cuts at: 3013 3320 4117 4567 5361 5381 3013
Size: 307 797 450 794 20 4683
Fragments arranged by size:
4683 797 794 450 307 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7051
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7016
DraI TTT'AAA
Cuts at: 4355 4355
Size: 7051
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6058 809
Size: 507 4742 1802
Fragments arranged by size:
4742 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4498 5387 7016
Size: 54 1425 50 275 18 2668 889 1629
Cuts at: 7016 8
Size: 43
Fragments arranged by size:
2668 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4498 1537
Size: 275 2686 4090
Fragments arranged by size:
4090 2686 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4981 1353
Size: 3628 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2693 2741 3212 3464 3632
Size: 1064 138 435 669 48 471 252 168
Cuts at: 3632 3800 4049 5638 6012 6158 387
Size: 168 249 1589 374 146 1280
Fragments arranged by size:
1589 1280 1064 669 471 435 374 252
249 168 168 146 138 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2191 2377 2986 3201 3500 3644 3668
Size: 393 559 186 609 215 299 144 24
Cuts at: 3668 3680 3836 4085 5114 5711 6498 6766 1239
Size: 12 156 249 1029 597 787 268 1524
Fragments arranged by size:
1524 1029 787 609 597 559 393 299
268 249 215 186 156 144 24 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2403 3044 3239 2403
Size: 641 195 6215
Fragments arranged by size:
6215 641 195
EcoO109I rG'GnC_Cy
Cuts at: 2504 2652 2800 2801 2997 3104 3105 3609 3861
Size: 148 148 1 196 107 1 504 252
Cuts at: 3861 4090 4194 2504
Size: 229 104 5361
Fragments arranged by size:
5361 504 252 229 196 148 148 107
104 1 1
EcoRI G'AATT_C
Cuts at: 2320 4360 4738 2320
Size: 2040 378 4633
Fragments arranged by size:
4633 2040 378
FseI GG_CCGG'CC
Cuts at: 2975 2975
Size: 7051
FspI TGC'GCA
Cuts at: 3204 5557 3204
Size: 2353 4698
FspAI rTGC'GCAy
Cuts at: 3204 3204
Size: 7051
HaeII r_GCGC'y
Cuts at: 1678 2917 3086 3136 3150 3220 3640 3844 3892
Size: 1239 169 50 14 70 420 204 48
Cuts at: 3892 6198 1678
Size: 2306 2531
Fragments arranged by size:
2531 2306 1239 420 204 169 70 50
48 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5692 5724 5766 5798 1373
Size: 32 135 32 4120 32 42 32 2626
Fragments arranged by size:
4120 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4395 4641 669
Size: 988 281 2457 246 3079
Fragments arranged by size:
3079 2457 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4241 748
Size: 3493 3558
HpaI GTT'AAC
Cuts at: 4641 4641
Size: 7051
KpnI G_GTAC'C
Cuts at: 4376 4376
Size: 7051
MfeI C'AATT_G
Cuts at: 4650 4650
Size: 7051
MluI A'CGCG_T
Cuts at: 6927 6927
Size: 7051
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2860 6165 6349 1305
Size: 1555 3305 184 2007
Fragments arranged by size:
3305 2007 1555 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5229
Fragments arranged by size:
5229 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5009 5368 5527 518
Size: 648 3843 359 159 2042
Fragments arranged by size:
3843 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2293 2994 3348 3406 3421 3600 3768 3826
Size: 687 701 354 58 15 179 168 58
Cuts at: 3826 3925 3936 4075 4467 5144 6292 6537 1606
Size: 99 11 139 392 677 1148 245 2120
Fragments arranged by size:
2120 1148 701 687 677 392 354 245
179 168 139 99 58 58 15 11
NaeI GCC'GGC
Cuts at: 2973 2973
Size: 7051
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5560
Fragments arranged by size:
5560 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7051
NgoMIV G'CCGG_C
Cuts at: 2971 2971
Size: 7051
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7051
NotI GC'GGCC_GC
Cuts at: 4498 4498
Size: 7051
NsiI A_TGCA'T
Cuts at: 2268 2268
Size: 7051
NspI r_CATG'y
Cuts at: 1132 1229 2114 3490 4162 4409 5954 1132
Size: 97 885 1376 672 247 1545 2229
Fragments arranged by size:
2229 1545 1376 885 672 247 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7051
PciI A'CATG_T
Cuts at: 5950 5950
Size: 7051
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3461 1108
Size: 2353 4698
PfoI T'CCnGG_A
Cuts at: 2180 2180
Size: 7051
PmeI GTTT'AAAC
Cuts at: 4355 4355
Size: 7051
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3260 3292 3512 3544 3680 3712 5120 5152 6633
Size: 32 220 32 136 32 1408 32 1481
Cuts at: 6633 6665 3260
Size: 32 3646
Fragments arranged by size:
3646 1481 1408 220 136 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2504 2504
Size: 7051
PshAI GACnn'nnGTC
Cuts at: 1613 2138 1613
Size: 525 6526
PsiI TTA'TAA
Cuts at: 4621 4621
Size: 7051
PspOMI G'GGCC_C
Cuts at: 2800 3104 2800
Size: 304 6747
PstI C_TGCA'G
Cuts at: 830 3090 3170 3243 3287 4403 830
Size: 2260 80 73 44 1116 3478
Fragments arranged by size:
3478 2260 1116 80 73 44
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7051
PvuII CAG'CTG
Cuts at: 2293 3406 3826 3925 4075 2293
Size: 1113 420 99 150 5269
Fragments arranged by size:
5269 1113 420 150 99
SacII CC_GC'GG
Cuts at: 2995 2995
Size: 7051
SalI G'TCGA_C
Cuts at: 1936 4393 1936
Size: 2457 4594
SbfI CC_TGCA'GG
Cuts at: 3170 3287 4403 3170
Size: 117 1116 5818
Fragments arranged by size:
5818 1116 117
ScaI AGT'ACT
Cuts at: 5299 7011 5299
Size: 1712 5339
SfcI C'TryA_G
Cuts at: 826 1045 3086 3166 3239 3283 3535 4399 5534
Size: 219 2041 80 73 44 252 864 1135
Cuts at: 5534 6215 6406 826
Size: 681 191 1471
Fragments arranged by size:
2041 1471 1135 864 681 252 219 191
80 73 44
SfiI GGCCn_nnn'nGGCC
Cuts at: 4192 4192
Size: 7051
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7051
SgrAI Cr'CCGG_yG
Cuts at: 1700 2946 1700
Size: 1246 5805
SmaI CCC'GGG
Cuts at: 1872 4378 1872
Size: 2506 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5155 6056 6318 820
Size: 197 926 3212 901 262 1553
Fragments arranged by size:
3212 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4842 493
Size: 4349 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7051
SphI G_CATG'C
Cuts at: 1132 3490 4409 1132
Size: 2358 919 3774
Fragments arranged by size:
3774 2358 919
SspI AAT'ATT
Cuts at: 5 52 4975 5
Size: 47 4923 2081
Fragments arranged by size:
4923 2081 47
StuI AGG'CCT
Cuts at: 1517 2398 4111 1517
Size: 881 1713 4457
Fragments arranged by size:
4457 1713 881
StyI C'CwwG_G
Cuts at: 513 1065 2004 2404 2567 4490 513
Size: 552 939 400 163 1923 3074
Fragments arranged by size:
3074 1923 939 552 400 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2573 3575 5078 5095 5248 5433 5878
Size: 1255 993 1002 1503 17 153 185 445
Cuts at: 5878 325
Size: 1498
Fragments arranged by size:
1503 1498 1255 1002 993 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4261 5297 7009
Size: 274 80 33 51 1443 2284 1036 1712
Cuts at: 7009 96
Size: 138
Fragments arranged by size:
2284 1712 1443 1036 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4256 4288 7011 7043 4256
Size: 32 2723 32 4264
Fragments arranged by size:
4264 2723 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7051
XbaI T'CTAG_A
Cuts at: 2080 2146 4387 2080
Size: 66 2241 4744
Fragments arranged by size:
4744 2241 66
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2372 2372
Size: 7051
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7051
XmaI C'CCGG_G
Cuts at: 1870 4376 1870
Size: 2506 4545
XmnI GAAnn'nnTTC
Cuts at: 3359 4223 5180 3359
Size: 864 957 5230
Fragments arranged by size:
5230 957 864
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2539 4859 276
Size: 53 83 186 936 1005 2320 2468
Fragments arranged by size:
2468 2320 1005 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BsiWI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FalI KasI NarI NruI PacI
PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfoI SrfI SwaI