Restriction map for
pFN21AB8397
(Circular) (Six-base) MAPSORT of: pFN21AB8397.seq Check: 3788 from: 1 to: 6091
pFN21AB8397 6091 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3901 278
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2985 3434 1937
Size: 1048 449 4594
Fragments arranged by size:
4594 1048 449
Acc65I G'GTAC_C
Cuts at: 3412 3412
Size: 6091
AclI AA'CG_TT
Cuts at: 1563 3390 4218 4591 1563
Size: 1827 828 373 3063
Fragments arranged by size:
3063 1827 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2561 3099 4154 5538 5806 1632
Size: 929 538 1055 1384 268 1917
Fragments arranged by size:
1917 1384 1055 929 538 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5894
AflIII A'CryG_T
Cuts at: 1203 2747 4990 5967 1203
Size: 1544 2243 977 1327
Fragments arranged by size:
2243 1544 1327 977
AgeI A'CCGG_T
Cuts at: 4922 4922
Size: 6091
AhdI GACnn_n'nnGTC
Cuts at: 4820 4820
Size: 6091
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6091
AlwNI CAG_nnn'CTG
Cuts at: 3834 5406 3834
Size: 1572 4519
ApaLI G'TGCA_C
Cuts at: 4148 5304 4148
Size: 1156 4935
ApoI r'AATT_y
Cuts at: 1493 3095 3317 3400 3626 3778 5887 1493
Size: 1602 222 83 226 152 2109 1697
Fragments arranged by size:
2109 1697 1602 226 222 152 83
AseI AT'TA_AT
Cuts at: 160 1032 3074 3080 4645 160
Size: 872 2042 6 1565 1606
Fragments arranged by size:
2042 1606 1565 872 6
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
AvaI C'yCGr_G
Cuts at: 1870 1943 3416 1870
Size: 73 1473 4545
Fragments arranged by size:
4545 1473 73
AvrII C'CTAG_G
Cuts at: 2998 2998
Size: 6091
BamHI G'GATC_C
Cuts at: 3154 3421 3788 3154
Size: 267 367 5457
Fragments arranged by size:
5457 367 267
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2944 3412 4867 618
Size: 325 217 627 1157 468 1455 1842
Fragments arranged by size:
1842 1455 1157 627 468 325 217
BanII G_rGCy'C
Cuts at: 1391 3144 3410 1391
Size: 1753 266 4072
Fragments arranged by size:
4072 1753 266
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2099 2921 3093 928
Size: 971 200 822 172 3926
Fragments arranged by size:
3926 971 822 200 172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4282 4316 1903
Size: 34 2345 34 3678
Fragments arranged by size:
3678 2345 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3983 5193 3983
Size: 1210 4881
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6091
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4702 136
Size: 107 122 71 631 605 3030 1525
Fragments arranged by size:
3030 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6086 6086
Size: 6091
BlpI GC'TnA_GC
Cuts at: 3508 3508
Size: 6091
Bme1580I G_kGCm'C
Cuts at: 1790 4152 5308 1790
Size: 2362 1156 2573
Fragments arranged by size:
2573 2362 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6091
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3822 4780 442
Size: 416 53 83 399 24 2405 958 1753
Fragments arranged by size:
2405 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2089 2219 2262 2916 3243 4751 1239
Size: 850 130 43 654 327 1508 2579
Fragments arranged by size:
2579 1508 850 654 327 130 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2115 2804 3261 4216 5081 5379 2115
Size: 689 457 955 865 298 2827
Fragments arranged by size:
2827 955 865 689 457 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4754 882
Size: 617 3255 2219
Fragments arranged by size:
3255 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3882 493
Size: 711 2678 2702
Fragments arranged by size:
2702 2678 711
BsaBI GATnn'nnATC
Cuts at: 6085 6085
Size: 6091
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3898 4280 4915 275
Size: 53 83 186 936 2365 382 635 1451
Fragments arranged by size:
2365 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2795 4524 4922 5196 5343 5754 2795
Size: 1729 398 274 147 411 3132
Fragments arranged by size:
3132 1729 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5866
Fragments arranged by size:
5866 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2360 1186
Size: 1174 4917
BseYI C'CCAG_C
Cuts at: 3382 5294 5790 3382
Size: 1912 496 3683
Fragments arranged by size:
3683 1912 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2781 1163
Size: 573 1045 4473
Fragments arranged by size:
4473 1045 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3541 4302 5330 1540
Size: 275 186 1540 761 1028 2301
Fragments arranged by size:
2301 1540 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4152 4237 5308 4152
Size: 85 1071 4935
Fragments arranged by size:
4935 1071 85
BsmI GAATG_Cn'
Cuts at: 3602 3695 3602
Size: 93 5998
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3144 3410 4152 4237 5308 1391
Size: 399 1354 266 742 85 1071 2174
Fragments arranged by size:
2174 1354 1071 742 399 266 85
BspEI T'CCGG_A
Cuts at: 2795 5754 2795
Size: 2959 3132
BspHI T'CATG_A
Cuts at: 3980 5710 3980
Size: 1730 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3446 844
Size: 2602 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2725 3978 1394
Size: 429 902 1253 3507
Fragments arranged by size:
3507 1253 902 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3128 4586 4760 65
Size: 1183 1880 1458 174 1396
Fragments arranged by size:
1880 1458 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4735 4922 1700
Size: 110 2925 187 2869
Fragments arranged by size:
2925 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6091
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6091
BssSI C'ACGA_G
Cuts at: 4151 5163 4151
Size: 1012 5079
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6091
BstBI TT'CG_AA
Cuts at: 4895 4895
Size: 6091
BstEII G'GTnAC_C
Cuts at: 1184 3033 1184
Size: 1849 4242
BstYI r'GATC_y
Cuts at: 1972 3154 3421 3788 4173 4190 5642 5728 5740
Size: 1182 267 367 385 17 1452 86 12
Cuts at: 5740 6086 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1182 385 367 346 267 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5539
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2025 487
Size: 683 56 799 4553
Fragments arranged by size:
4553 799 683 56
BtsI GCAGTG_nn'
Cuts at: 3607 4401 4421 3607
Size: 794 20 5277
Fragments arranged by size:
5277 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6091
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6056
DraI TTT'AAA
Cuts at: 3271 3395 3271
Size: 124 5967
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5098 809
Size: 507 3782 1802
Fragments arranged by size:
3782 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2851 2974 3538
Size: 54 1425 50 275 18 1021 123 564
Cuts at: 3538 4427 6056 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1021 889 564 275 123 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3538 1537
Size: 275 1726 4090
Fragments arranged by size:
4090 1726 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2039 2337 2391 3118 4021 1353
Size: 686 298 54 727 903 3423
Fragments arranged by size:
3423 903 727 686 298 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4678 5052 5198 387
Size: 1064 138 3089 374 146 1280
Fragments arranged by size:
3089 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2089 2219 2262 2561 2916 3099 3243
Size: 393 457 130 43 299 355 183 144
Cuts at: 3243 4154 4751 5538 5806 1239
Size: 911 597 787 268 1524
Fragments arranged by size:
1524 911 787 597 457 393 355 299
268 183 144 130 43
EcoO109I rG'GnC_Cy
Cuts at: 2995 2995
Size: 6091
EcoRI G'AATT_C
Cuts at: 3400 3778 3400
Size: 378 5713
FspI TGC'GCA
Cuts at: 4597 4597
Size: 6091
HaeII r_GCGC'y
Cuts at: 1678 5238 1678
Size: 3560 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3110 3139 3142 3171 4732
Size: 32 135 32 1538 29 3 29 1561
Cuts at: 4732 4764 4806 4838 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1561 1538 135 42 32 32 32
32 29 29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3435 3681 669
Size: 988 281 1497 246 3079
Fragments arranged by size:
3079 1497 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3254 748
Size: 2506 3585
HpaI GTT'AAC
Cuts at: 3681 3681
Size: 6091
KpnI G_GTAC'C
Cuts at: 3416 3416
Size: 6091
MfeI C'AATT_G
Cuts at: 3690 3690
Size: 6091
MluI A'CGCG_T
Cuts at: 5967 5967
Size: 6091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2390 2805 5205 5389 1305
Size: 1085 415 2400 184 2007
Fragments arranged by size:
2400 2007 1085 415 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2853 10
Size: 54 1425 343 1021 3248
Fragments arranged by size:
3248 1425 1021 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4049 4408 4567 518
Size: 648 2883 359 159 2042
Fragments arranged by size:
2883 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3507 4184 5332 5577 1606
Size: 1901 677 1148 245 2120
Fragments arranged by size:
2120 1901 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5539
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6091
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6091
NotI GC'GGCC_GC
Cuts at: 3538 3538
Size: 6091
NspI r_CATG'y
Cuts at: 1132 1229 3382 3449 4994 1132
Size: 97 2153 67 1545 2229
Fragments arranged by size:
2229 2153 1545 97 67
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6091
PciI A'CATG_T
Cuts at: 4990 4990
Size: 6091
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6091
PmeI GTTT'AAAC
Cuts at: 3395 3395
Size: 6091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2714 2746 4160 4192 5673 5705 2714
Size: 32 1414 32 1481 32 3100
Fragments arranged by size:
3100 1481 1414 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6091
PsiI TTA'TAA
Cuts at: 2222 3661 2222
Size: 1439 4652
PstI C_TGCA'G
Cuts at: 830 2334 3443 830
Size: 1504 1109 3478
Fragments arranged by size:
3478 1504 1109
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6091
SalI G'TCGA_C
Cuts at: 1936 3433 1936
Size: 1497 4594
SbfI CC_TGCA'GG
Cuts at: 3443 3443
Size: 6091
ScaI AGT'ACT
Cuts at: 4339 6051 4339
Size: 1712 4379
SfcI C'TryA_G
Cuts at: 826 1045 2316 2330 3439 4574 5255 5446 826
Size: 219 1271 14 1109 1135 681 191 1471
Fragments arranged by size:
1471 1271 1135 1109 681 219 191 14
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6091
SmaI CCC'GGG
Cuts at: 1872 3418 1872
Size: 1546 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2130 2819 3276 4195 5096 5358
Size: 197 926 187 689 457 919 901 262
Cuts at: 5358 820
Size: 1553
Fragments arranged by size:
1553 926 919 901 689 457 262 197
187
SnaBI TAC'GTA
Cuts at: 493 3882 493
Size: 3389 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6091
SphI G_CATG'C
Cuts at: 1132 3382 3449 1132
Size: 2250 67 3774
Fragments arranged by size:
3774 2250 67
SspI AAT'ATT
Cuts at: 5 52 2642 3078 4015 5
Size: 47 2590 436 937 2081
Fragments arranged by size:
2590 2081 937 436 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6091
StyI C'CwwG_G
Cuts at: 513 1065 2778 2998 3169 3530 513
Size: 552 1713 220 171 361 3074
Fragments arranged by size:
3074 1713 552 361 220 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4118 4135 4288 4473 4918 325
Size: 1255 2538 17 153 185 445 1498
Fragments arranged by size:
2538 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4337 6049 96
Size: 274 80 33 51 1443 2360 1712 138
Fragments arranged by size:
2360 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2602 2634 2790 2822 6051 6083 2602
Size: 32 156 32 3229 32 2610
Fragments arranged by size:
3229 2610 156 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6091
XbaI T'CTAG_A
Cuts at: 3427 3427
Size: 6091
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6091
XmaI C'CCGG_G
Cuts at: 1870 3416 1870
Size: 1546 4545
XmnI GAAnn'nnTTC
Cuts at: 2918 4220 2918
Size: 1302 4789
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3899 276
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI