Restriction map for
pFN21AB8390
(Circular) (Six-base) MAPSORT of: pFN21AB8390.seq Check: 3947 from: 1 to: 5899
pFN21AB8390 5899 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2161 2313 2534 2961 3051 2161
Size: 152 221 427 90 5009
Fragments arranged by size:
5009 427 221 152 90
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2936 3709 278
Size: 53 83 186 936 1400 773 2468
Fragments arranged by size:
2468 1400 936 773 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3242 1937
Size: 1305 4594
Acc65I G'GTAC_C
Cuts at: 3220 3220
Size: 5899
AclI AA'CG_TT
Cuts at: 1563 4026 4399 1563
Size: 2463 373 3063
Fragments arranged by size:
3063 2463 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2963 3962 5346 5614 1632
Size: 1331 999 1384 268 1917
Fragments arranged by size:
1917 1384 1331 999 268
AfeI AGC'GCT
Cuts at: 3068 3095 3068
Size: 27 5872
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5702
AflIII A'CryG_T
Cuts at: 1203 2892 4798 5775 1203
Size: 1689 1906 977 1327
Fragments arranged by size:
1906 1689 1327 977
AgeI A'CCGG_T
Cuts at: 4730 4730
Size: 5899
AhdI GACnn_n'nnGTC
Cuts at: 4628 4628
Size: 5899
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5899
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5899
AlwNI CAG_nnn'CTG
Cuts at: 3642 5214 3642
Size: 1572 4327
ApaI G_GGCC'C
Cuts at: 2653 2735 2653
Size: 82 5817
ApaLI G'TGCA_C
Cuts at: 3956 5112 3956
Size: 1156 4743
ApoI r'AATT_y
Cuts at: 1493 3208 3434 3586 5695 1493
Size: 1715 226 152 2109 1697
Fragments arranged by size:
2109 1715 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4453 160
Size: 872 3421 1606
Fragments arranged by size:
3421 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
AvaI C'yCGr_G
Cuts at: 1870 1943 2401 2861 3080 3224 1870
Size: 73 458 460 219 144 4545
Fragments arranged by size:
4545 460 458 219 144 73
BamHI G'GATC_C
Cuts at: 3229 3596 3229
Size: 367 5532
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2152 2173 2591 2636 2814
Size: 325 217 627 365 21 418 45 178
Cuts at: 2814 2855 3039 3220 4675 618
Size: 41 184 181 1455 1842
Fragments arranged by size:
1842 1455 627 418 365 325 217 184
181 178 45 41 21
BanII G_rGCy'C
Cuts at: 1391 2198 2653 2735 3218 1391
Size: 807 455 82 483 4072
Fragments arranged by size:
4072 807 483 455 82
BbeI G_GCGC'C
Cuts at: 2156 2595 2640 2859 2156
Size: 439 45 219 5196
Fragments arranged by size:
5196 439 219 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2303 2936 928
Size: 971 404 633 3891
Fragments arranged by size:
3891 971 633 404
BbvCI CC'TCA_GC
Cuts at: 3162 3162
Size: 5899
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4090 4124 1903
Size: 34 2153 34 3678
Fragments arranged by size:
3678 2153 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3791 5001 3791
Size: 1210 4689
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5899
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2015 2133 2142
Size: 107 122 71 631 605 343 118 9
Cuts at: 2142 2634 2681 2795 2864 4510 136
Size: 492 47 114 69 1646 1525
Fragments arranged by size:
1646 1525 631 605 492 343 122 118
114 107 71 69 47 9
BglII A'GATC_T
Cuts at: 5894 5894
Size: 5899
BlpI GC'TnA_GC
Cuts at: 3316 3316
Size: 5899
Bme1580I G_kGCm'C
Cuts at: 1790 2178 2448 2653 2735 3960 5116 1790
Size: 388 270 205 82 1225 1156 2573
Fragments arranged by size:
2573 1225 1156 388 270 205 82
BmgBI CAC'GTC
Cuts at: 1318 3127 1318
Size: 1809 4090
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3630 4588 442
Size: 416 53 83 399 24 2213 958 1753
Fragments arranged by size:
2213 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5899
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2036 2188 4559 1239
Size: 797 152 2371 2579
Fragments arranged by size:
2579 2371 797 152
Bpu10I CC'TnA_GC
Cuts at: 3162 3162
Size: 5899
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4024 4889 5187 4024
Size: 865 298 4736
Fragments arranged by size:
4736 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4562 882
Size: 617 3063 2219
Fragments arranged by size:
3063 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2443 2893 2902 3690 493
Size: 711 1239 450 9 788 2702
Fragments arranged by size:
2702 1239 788 711 450 9
BsaBI GATnn'nnATC
Cuts at: 5893 5893
Size: 5899
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2153 2288 2592 2637
Size: 53 83 186 936 620 135 304 45
Cuts at: 2637 2856 2933 3706 4088 4723 275
Size: 219 77 773 382 635 1451
Fragments arranged by size:
1451 936 773 635 620 382 304 219
186 135 83 77 53 45
BsaWI w'CCGG_w
Cuts at: 2025 4332 4730 5004 5151 5562 2025
Size: 2307 398 274 147 411 2362
Fragments arranged by size:
2362 2307 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5674
Fragments arranged by size:
5674 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5899
BseYI C'CCAG_C
Cuts at: 2066 2191 2273 5102 5598 2066
Size: 125 82 2829 496 2367
Fragments arranged by size:
2829 2367 496 125 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5326
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2685 2700 3349 4110 5138 1540
Size: 275 186 684 15 649 761 1028 2301
Fragments arranged by size:
2301 1028 761 684 649 275 186 15
BsiHKAI G_wGCw'C
Cuts at: 3960 4045 5116 3960
Size: 85 1071 4743
Fragments arranged by size:
4743 1071 85
BsmI GAATG_Cn'
Cuts at: 3410 3503 3410
Size: 93 5806
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2178 2198 2448 2653 2735 3218 3960
Size: 399 388 20 250 205 82 483 742
Cuts at: 3960 4045 5116 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 483 399 388 250 205
85 82 20
BspEI T'CCGG_A
Cuts at: 2025 5562 2025
Size: 3537 2362
BspHI T'CATG_A
Cuts at: 3788 5518 3788
Size: 1730 4169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2161 2313 2496 2534 2961 3051 3254 844
Size: 1317 152 183 38 427 90 203 3489
Fragments arranged by size:
3489 1317 427 203 183 152 90 38
BsrBI CCG'CTC
Cuts at: 1394 1823 3786 1394
Size: 429 1963 3507
Fragments arranged by size:
3507 1963 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2105 4394 4568 65
Size: 1183 857 2289 174 1396
Fragments arranged by size:
2289 1396 1183 857 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2236 2383 4543 4730 1700
Size: 110 426 147 2160 187 2869
Fragments arranged by size:
2869 2160 426 187 147 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5899
BssHII G'CGCG_C
Cuts at: 1925 2430 1925
Size: 505 5394
BssSI C'ACGA_G
Cuts at: 2330 3959 4971 2330
Size: 1629 1012 3258
Fragments arranged by size:
3258 1629 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5899
BstBI TT'CG_AA
Cuts at: 4703 4703
Size: 5899
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5899
BstYI r'GATC_y
Cuts at: 1972 3229 3596 3981 3998 5450 5536 5548 5894
Size: 1257 367 385 17 1452 86 12 346
Cuts at: 5894 1972
Size: 1977
Fragments arranged by size:
1977 1452 1257 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2010 2219 2567 2802 513
Size: 552 939 6 209 348 235 3610
Fragments arranged by size:
3610 939 552 348 235 209 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5160
Fragments arranged by size:
5160 683 56
BtsI GCAGTG_nn'
Cuts at: 2255 3415 4209 4229 2255
Size: 1160 794 20 3925
Fragments arranged by size:
3925 1160 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5899
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5864
DraI TTT'AAA
Cuts at: 3203 3203
Size: 5899
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4906 809
Size: 507 3590 1802
Fragments arranged by size:
3590 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2016 2112
Size: 54 1425 50 275 18 177 9 96
Cuts at: 2112 2134 2682 2697 2799 2805 3346 4235 5864
Size: 22 548 15 102 6 541 889 1629
Cuts at: 5864 8
Size: 43
Fragments arranged by size:
1629 1425 889 548 541 275 177 102
96 54 50 43 22 18 15 9
6
EagI C'GGCC_G
Cuts at: 1537 1812 2682 2697 3346 1537
Size: 275 870 15 649 4090
Fragments arranged by size:
4090 870 649 275 15
EarI CTCTTCn'nnn_
Cuts at: 1353 2216 3002 3829 1353
Size: 863 786 827 3423
Fragments arranged by size:
3423 863 827 786
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2487 2882 4486 4860 5006 387
Size: 1064 138 898 395 1604 374 146 1280
Fragments arranged by size:
1604 1280 1064 898 395 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2188 2963 3962 4559 5346 5614
Size: 393 404 152 775 999 597 787 268
Cuts at: 5614 1239
Size: 1524
Fragments arranged by size:
1524 999 787 775 597 404 393 268
152
EcoO109I rG'GnC_Cy
Cuts at: 2604 2649 2650 2788 3193 2604
Size: 45 1 138 405 5310
Fragments arranged by size:
5310 405 138 45 1
EcoRI G'AATT_C
Cuts at: 3208 3586 3208
Size: 378 5521
FspI TGC'GCA
Cuts at: 2328 2748 3104 4405 2328
Size: 420 356 1301 3822
Fragments arranged by size:
3822 1301 420 356
HaeII r_GCGC'y
Cuts at: 1678 2073 2156 2595 2640 2859 3070 3097 5046
Size: 395 83 439 45 219 211 27 1949
Cuts at: 5046 1678
Size: 2531
Fragments arranged by size:
2531 1949 439 395 219 211 83 45
27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4540 4572 4614 4646 1373
Size: 32 135 32 2968 32 42 32 2626
Fragments arranged by size:
2968 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3243 3489 669
Size: 988 281 1305 246 3079
Fragments arranged by size:
3079 1305 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5899
HpaI GTT'AAC
Cuts at: 3489 3489
Size: 5899
KasI G'GCGC_C
Cuts at: 2152 2591 2636 2855 2152
Size: 439 45 219 5196
Fragments arranged by size:
5196 439 219 45
KpnI G_GTAC'C
Cuts at: 3224 3224
Size: 5899
MfeI C'AATT_G
Cuts at: 3498 3498
Size: 5899
MluI A'CGCG_T
Cuts at: 5775 5775
Size: 5899
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2508 2718 5013 5197 1305
Size: 1203 210 2295 184 2007
Fragments arranged by size:
2295 2007 1203 210 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4077
Fragments arranged by size:
4077 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3857 4216 4375 518
Size: 648 2691 359 159 2042
Fragments arranged by size:
2691 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2012 2427 2687 2823 2829 3037 3315 3992
Size: 406 415 260 136 6 208 278 677
Cuts at: 3992 5140 5385 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 415 406 278 260 245
208 136 6
NaeI GCC'GGC
Cuts at: 2238 2238
Size: 5899
NarI GG'CG_CC
Cuts at: 2153 2592 2637 2856 2153
Size: 439 45 219 5196
Fragments arranged by size:
5196 439 219 45
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4408
Fragments arranged by size:
4408 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5899
NgoMIV G'CCGG_C
Cuts at: 2236 2236
Size: 5899
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5899
NotI GC'GGCC_GC
Cuts at: 2682 2697 3346 2682
Size: 15 649 5235
Fragments arranged by size:
5235 649 15
NruI TCG'CGA
Cuts at: 3119 3119
Size: 5899
NspI r_CATG'y
Cuts at: 1132 1229 2236 3257 4802 1132
Size: 97 1007 1021 1545 2229
Fragments arranged by size:
2229 1545 1021 1007 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5899
PciI A'CATG_T
Cuts at: 4798 4798
Size: 5899
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5899
PfoI T'CCnGG_A
Cuts at: 2052 2617 2052
Size: 565 5334
PmeI GTTT'AAAC
Cuts at: 3203 3203
Size: 5899
PmlI CAC'GTG
Cuts at: 2893 2902 2893
Size: 9 5890
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3968 4000 5481 5513 3968
Size: 32 1481 32 4354
Fragments arranged by size:
4354 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3193 3193
Size: 5899
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5899
PsiI TTA'TAA
Cuts at: 3469 3469
Size: 5899
PspOMI G'GGCC_C
Cuts at: 2649 2731 2649
Size: 82 5817
PspXI vC'TCGA_Gb
Cuts at: 2401 2401
Size: 5899
PstI C_TGCA'G
Cuts at: 830 2043 2172 2310 2781 3036 3251 830
Size: 1213 129 138 471 255 215 3478
Fragments arranged by size:
3478 1213 471 255 215 138 129
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5899
PvuII CAG'CTG
Cuts at: 2427 2427
Size: 5899
SacII CC_GC'GG
Cuts at: 2013 2013
Size: 5899
SalI G'TCGA_C
Cuts at: 1936 3241 1936
Size: 1305 4594
SapI GCTCTTCn'nnn_
Cuts at: 2216 3002 2216
Size: 786 5113
SbfI CC_TGCA'GG
Cuts at: 2172 3251 2172
Size: 1079 4820
ScaI AGT'ACT
Cuts at: 4147 5859 4147
Size: 1712 4187
SexAI A'CCwGG_T
Cuts at: 3195 3195
Size: 5899
SfcI C'TryA_G
Cuts at: 826 1045 2039 2122 2168 2306 2777 3032 3247
Size: 219 994 83 46 138 471 255 215
Cuts at: 3247 4382 5063 5254 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 994 681 471 255 219 215
191 138 83 46
SfiI GGCCn_nnn'nGGCC
Cuts at: 2015 2015
Size: 5899
SfoI GGC'GCC
Cuts at: 2154 2593 2638 2857 2154
Size: 439 45 219 5196
Fragments arranged by size:
5196 439 219 45
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5899
SmaI CCC'GGG
Cuts at: 1872 3226 1872
Size: 1354 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2401 4003 4904 5166 820
Size: 197 926 458 1602 901 262 1553
Fragments arranged by size:
1602 1553 926 901 458 262 197
SnaBI TAC'GTA
Cuts at: 493 3690 493
Size: 3197 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5899
SphI G_CATG'C
Cuts at: 1132 3257 1132
Size: 2125 3774
SspI AAT'ATT
Cuts at: 5 52 3823 5
Size: 47 3771 2081
Fragments arranged by size:
3771 2081 47
StuI AGG'CCT
Cuts at: 1517 2045 2721 1517
Size: 528 676 4695
Fragments arranged by size:
4695 676 528
StyI C'CwwG_G
Cuts at: 513 1065 2004 2784 3338 513
Size: 552 939 780 554 3074
Fragments arranged by size:
3074 939 780 554 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2557 3926 3943 4096 4281 4726 325
Size: 1255 977 1369 17 153 185 445 1498
Fragments arranged by size:
1498 1369 1255 977 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4145 5857 96
Size: 274 80 33 51 1443 2168 1712 138
Fragments arranged by size:
2168 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5859 5891 5859
Size: 32 5867
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5899
XbaI T'CTAG_A
Cuts at: 3235 3235
Size: 5899
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2791 2011
Size: 780 5119
XhoI C'TCGA_G
Cuts at: 1943 2401 1943
Size: 458 5441
XmaI C'CCGG_G
Cuts at: 1870 3224 1870
Size: 1354 4545
XmnI GAAnn'nnTTC
Cuts at: 3011 4028 3011
Size: 1017 4882
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2934 3707 276
Size: 53 83 186 936 1400 773 2468
Fragments arranged by size:
2468 1400 936 773 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NsiI PacI PsrI RsrII SacI SanDI
SrfI SwaI