Restriction map for
pFN21AB8388
(Circular) (Six-base) MAPSORT of: pFN21AB8388.seq Check: 7326 from: 1 to: 6451
pFN21AB8388 6451 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2419 2419
Size: 6451
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2771 4261 278
Size: 53 83 186 936 1235 1490 2468
Fragments arranged by size:
2468 1490 1235 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3794 1937
Size: 1857 4594
Acc65I G'GTAC_C
Cuts at: 3772 3772
Size: 6451
AclI AA'CG_TT
Cuts at: 1563 4578 4951 1563
Size: 3015 373 3063
Fragments arranged by size:
3063 3015 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2621 2637 2753 2837 3116 3336 4514 5898
Size: 989 16 116 84 279 220 1178 1384
Cuts at: 5898 6166 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1178 989 279 268 220 116
84 16
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6254
AflIII A'CryG_T
Cuts at: 1203 5350 6327 1203
Size: 4147 977 1327
Fragments arranged by size:
4147 1327 977
AgeI A'CCGG_T
Cuts at: 5282 5282
Size: 6451
AhdI GACnn_n'nnGTC
Cuts at: 5180 5180
Size: 6451
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6451
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6451
AlwNI CAG_nnn'CTG
Cuts at: 2344 2815 2890 3271 4194 5766 2344
Size: 471 75 381 923 1572 3029
Fragments arranged by size:
3029 1572 923 471 381 75
ApaI G_GGCC'C
Cuts at: 2707 3059 3615 2707
Size: 352 556 5543
Fragments arranged by size:
5543 556 352
ApaLI G'TGCA_C
Cuts at: 3482 3659 4508 5664 3482
Size: 177 849 1156 4269
Fragments arranged by size:
4269 1156 849 177
ApoI r'AATT_y
Cuts at: 1493 3760 3986 4138 6247 1493
Size: 2267 226 152 2109 1697
Fragments arranged by size:
2267 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5005 160
Size: 872 3973 1606
Fragments arranged by size:
3973 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6451
AvaI C'yCGr_G
Cuts at: 1870 1943 2651 2969 3776 1870
Size: 73 708 318 807 4545
Fragments arranged by size:
4545 807 708 318 73
BamHI G'GATC_C
Cuts at: 3781 4148 3781
Size: 367 6084
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2188 2227 2353 2378 2431
Size: 325 217 627 401 39 126 25 53
Cuts at: 2431 2608 3274 3448 3772 5227 618
Size: 177 666 174 324 1455 1842
Fragments arranged by size:
1842 1455 666 627 401 325 324 217
177 174 126 53 39 25
BanII G_rGCy'C
Cuts at: 1391 2707 2901 3059 3521 3615 3652 3770 1391
Size: 1316 194 158 462 94 37 118 4072
Fragments arranged by size:
4072 1316 462 194 158 118 94 37
BbeI G_GCGC'C
Cuts at: 2192 2357 2382 3452 2192
Size: 165 25 1070 5191
Fragments arranged by size:
5191 1070 165 25
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2771 3227 928
Size: 971 872 456 4152
Fragments arranged by size:
4152 971 872 456
BbvCI CC'TCA_GC
Cuts at: 2298 2556 2298
Size: 258 6193
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3385 3419 4642 4676 1903
Size: 34 1448 34 1223 34 3678
Fragments arranged by size:
3678 1448 1223 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4343 5553 4343
Size: 1210 5241
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6451
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2150 2422 3422
Size: 107 122 71 631 605 478 272 1000
Cuts at: 3422 3647 5062 136
Size: 225 1415 1525
Fragments arranged by size:
1525 1415 1000 631 605 478 272 225
122 107 71
BglII A'GATC_T
Cuts at: 6446 6446
Size: 6451
BlpI GC'TnA_GC
Cuts at: 2912 3068 3868 2912
Size: 156 800 5495
Fragments arranged by size:
5495 800 156
Bme1580I G_kGCm'C
Cuts at: 1790 2436 2707 3059 3474 3486 3615 3663 4512
Size: 646 271 352 415 12 129 48 849
Cuts at: 4512 5668 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 849 646 415 352 271 129
48 12
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6451
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3722 4182 5140
Size: 416 53 83 399 24 2305 460 958
Cuts at: 5140 442
Size: 1753
Fragments arranged by size:
2305 1753 958 460 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6451
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2045 2565 2954 3267 5111 1239
Size: 806 520 389 313 1844 2579
Fragments arranged by size:
2579 1844 806 520 389 313
Bpu10I CC'TnA_GC
Cuts at: 2298 2556 3063 3651 2298
Size: 258 507 588 5098
Fragments arranged by size:
5098 588 507 258
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2885 3725 4576 5441 5739 2885
Size: 840 851 865 298 3597
Fragments arranged by size:
3597 865 851 840 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2720 5114 882
Size: 617 1221 2394 2219
Fragments arranged by size:
2394 2219 1221 617
BsaAI yAC'GTr
Cuts at: 493 1204 2075 2864 4242 493
Size: 711 871 789 1378 2702
Fragments arranged by size:
2702 1378 871 789 711
BsaBI GATnn'nnATC
Cuts at: 6445 6445
Size: 6451
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2189 2354 2379 2768
Size: 53 83 186 936 656 165 25 389
Cuts at: 2768 3449 4258 4640 5275 275
Size: 681 809 382 635 1451
Fragments arranged by size:
1451 936 809 681 656 635 389 382
186 165 83 53 25
BsaWI w'CCGG_w
Cuts at: 4884 5282 5556 5703 6114 4884
Size: 398 274 147 411 5221
Fragments arranged by size:
5221 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2428 2458 2969 2999 3149
Size: 30 165 30 1025 30 511 30 150
Cuts at: 3149 3179 3503 3533 1178
Size: 30 324 30 4096
Fragments arranged by size:
4096 1025 511 324 165 150 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 3050 3231 1186
Size: 907 957 181 4406
Fragments arranged by size:
4406 957 907 181
BseYI C'CCAG_C
Cuts at: 2337 2417 2568 2590 3081 3116 3305 3428 3522
Size: 80 151 22 491 35 189 123 94
Cuts at: 3522 3669 5654 6150 2337
Size: 147 1985 496 2638
Fragments arranged by size:
2638 1985 496 491 189 151 147 123
94 80 35 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3218 1163
Size: 573 1482 4396
Fragments arranged by size:
4396 1482 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3603 3642 3901 4662 5690 1540
Size: 275 186 1602 39 259 761 1028 2301
Fragments arranged by size:
2301 1602 1028 761 275 259 186 39
BsiHKAI G_wGCw'C
Cuts at: 2074 2901 3486 3663 3693 4512 4597 5668 2074
Size: 827 585 177 30 819 85 1071 2857
Fragments arranged by size:
2857 1071 827 819 585 177 85 30
BsmI GAATG_Cn'
Cuts at: 3962 4055 3962
Size: 93 6358
BsmBI CGTCTCn'nnnn_
Cuts at: 2009 2009
Size: 6451
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2074 2436 2707 2901 3059 3474 3486
Size: 399 284 362 271 194 158 415 12
Cuts at: 3486 3521 3615 3652 3663 3693 3770 4512 4597
Size: 35 94 37 11 30 77 742 85
Cuts at: 4597 5668 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 415 399 362 284 271
194 158 94 85 77 37 35 30
12 11
BspEI T'CCGG_A
Cuts at: 6114 6114
Size: 6451
BspHI T'CATG_A
Cuts at: 4340 6070 4340
Size: 1730 4721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2419 3806 844
Size: 1575 1387 3489
Fragments arranged by size:
3489 1575 1387
BsrBI CCG'CTC
Cuts at: 1394 1823 2089 2854 4338 1394
Size: 429 266 765 1484 3507
Fragments arranged by size:
3507 1484 765 429 266
BsrDI GCAATG_nn'
Cuts at: 65 1248 4946 5120 65
Size: 1183 3698 174 1396
Fragments arranged by size:
3698 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2212 2224 2365 3250 3602 3637 5095
Size: 110 402 12 141 885 352 35 1458
Cuts at: 5095 5282 1700
Size: 187 2869
Fragments arranged by size:
2869 1458 885 402 352 187 141 110
35 12
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6451
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6451
BssSI C'ACGA_G
Cuts at: 2906 4511 5523 2906
Size: 1605 1012 3834
Fragments arranged by size:
3834 1605 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6451
BstBI TT'CG_AA
Cuts at: 5255 5255
Size: 6451
BstEII G'GTnAC_C
Cuts at: 1184 2284 3709 1184
Size: 1100 1425 3926
Fragments arranged by size:
3926 1425 1100
BstXI CCAn_nnnn'nTGG
Cuts at: 2140 2140
Size: 6451
BstYI r'GATC_y
Cuts at: 1972 3781 4148 4533 4550 6002 6088 6100 6446
Size: 1809 367 385 17 1452 86 12 346
Cuts at: 6446 1972
Size: 1977
Fragments arranged by size:
1977 1809 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2328 3168 3318 3546 3642 3705 513
Size: 552 1263 840 150 228 96 63 3259
Fragments arranged by size:
3259 1263 840 552 228 150 96 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3310 3388 3397 3547 487
Size: 683 56 2084 78 9 150 3391
Fragments arranged by size:
3391 2084 683 150 78 56 9
BtsI GCAGTG_nn'
Cuts at: 3558 3967 4761 4781 3558
Size: 409 794 20 5228
Fragments arranged by size:
5228 794 409 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6451
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6416
DraI TTT'AAA
Cuts at: 3755 3755
Size: 6451
DraIII CAC_nnn'GTG
Cuts at: 3169 3169
Size: 6451
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5458 809
Size: 507 4142 1802
Fragments arranged by size:
4142 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2142 3600 3639
Size: 54 1425 50 275 18 312 1458 39
Cuts at: 3639 3898 4787 6416 8
Size: 259 889 1629 43
Fragments arranged by size:
1629 1458 1425 889 312 275 259 54
50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 3600 3639 3898 1537
Size: 275 1788 39 259 4090
Fragments arranged by size:
4090 1788 275 259 39
EarI CTCTTCn'nnn_
Cuts at: 1353 2639 2813 4381 1353
Size: 1286 174 1568 3423
Fragments arranged by size:
3423 1568 1286 174
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5038 5412 5558 387
Size: 1064 138 3449 374 146 1280
Fragments arranged by size:
3449 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2899 2899
Size: 6451
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2565 2621 2637 2753 2837 2954
Size: 393 413 520 56 16 116 84 117
Cuts at: 2954 3116 3267 3336 4514 5111 5898 6166 1239
Size: 162 151 69 1178 597 787 268 1524
Fragments arranged by size:
1524 1178 787 597 520 413 393 268
162 151 117 116 84 69 56 16
EcoO109I rG'GnC_Cy
Cuts at: 2595 2703 3611 3612 2595
Size: 108 908 1 5434
Fragments arranged by size:
5434 908 108 1
EcoRI G'AATT_C
Cuts at: 3760 4138 3760
Size: 378 6073
FseI GG_CCGG'CC
Cuts at: 3606 3606
Size: 6451
FspI TGC'GCA
Cuts at: 4957 4957
Size: 6451
HaeII r_GCGC'y
Cuts at: 1678 2192 2357 2382 2537 2798 3452 5598 1678
Size: 514 165 25 155 261 654 2146 2531
Fragments arranged by size:
2531 2146 654 514 261 165 155 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2692 2724 2758 2790 2969
Size: 32 135 32 1120 32 34 32 179
Cuts at: 2969 3001 3061 3093 5092 5124 5166 5198 1373
Size: 32 60 32 1999 32 42 32 2626
Fragments arranged by size:
2626 1999 1120 179 135 60 42 34
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3592 3795 4041 669
Size: 988 281 1654 203 246 3079
Fragments arranged by size:
3079 1654 988 281 246 203
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6451
HpaI GTT'AAC
Cuts at: 4041 4041
Size: 6451
KasI G'GCGC_C
Cuts at: 2188 2353 2378 3448 2188
Size: 165 25 1070 5191
Fragments arranged by size:
5191 1070 165 25
KpnI G_GTAC'C
Cuts at: 3776 3776
Size: 6451
MfeI C'AATT_G
Cuts at: 4050 4050
Size: 6451
MluI A'CGCG_T
Cuts at: 6327 6327
Size: 6451
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5565 5749 1305
Size: 4260 184 2007
Fragments arranged by size:
4260 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2144 10
Size: 54 1425 343 312 4317
Fragments arranged by size:
4317 1425 343 312 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3479 4409 4768 4927 518
Size: 648 2313 930 359 159 2042
Fragments arranged by size:
2313 2042 930 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2330 2560 2615 2887 3526 3673 3867 4544
Size: 724 230 55 272 639 147 194 677
Cuts at: 4544 5692 5937 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 724 677 639 272 245 230
194 147 55
NaeI GCC'GGC
Cuts at: 2226 2367 3604 3639 2226
Size: 141 1237 35 5038
Fragments arranged by size:
5038 1237 141 35
NarI GG'CG_CC
Cuts at: 2189 2354 2379 3449 2189
Size: 165 25 1070 5191
Fragments arranged by size:
5191 1070 165 25
NcoI C'CATG_G
Cuts at: 513 1065 3705 513
Size: 552 2640 3259
Fragments arranged by size:
3259 2640 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6451
NgoMIV G'CCGG_C
Cuts at: 2224 2365 3602 3637 2224
Size: 141 1237 35 5038
Fragments arranged by size:
5038 1237 141 35
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6451
NotI GC'GGCC_GC
Cuts at: 3898 3898
Size: 6451
NspI r_CATG'y
Cuts at: 1132 1229 3809 5354 1132
Size: 97 2580 1545 2229
Fragments arranged by size:
2580 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6451
PciI A'CATG_T
Cuts at: 5350 5350
Size: 6451
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2483 2543 2923 3169 3271 3295 3676 1108
Size: 1375 60 380 246 102 24 381 3883
Fragments arranged by size:
3883 1375 381 380 246 102 60 24
PfoI T'CCnGG_A
Cuts at: 2040 2040
Size: 6451
PmeI GTTT'AAAC
Cuts at: 3755 3755
Size: 6451
PmlI CAC'GTG
Cuts at: 2075 2864 2075
Size: 789 5662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4520 4552 6033 6065 4520
Size: 32 1481 32 4906
Fragments arranged by size:
4906 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2595 2595
Size: 6451
PshAI GACnn'nnGTC
Cuts at: 1613 3707 1613
Size: 2094 4357
PsiI TTA'TAA
Cuts at: 4021 4021
Size: 6451
PspOMI G'GGCC_C
Cuts at: 2703 3055 3611 2703
Size: 352 556 5543
Fragments arranged by size:
5543 556 352
PstI C_TGCA'G
Cuts at: 830 3384 3803 830
Size: 2554 419 3478
Fragments arranged by size:
3478 2554 419
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6451
PvuII CAG'CTG
Cuts at: 2560 2615 3526 3673 2560
Size: 55 911 147 5338
Fragments arranged by size:
5338 911 147 55
SacI G_AGCT'C
Cuts at: 2901 2901
Size: 6451
SacII CC_GC'GG
Cuts at: 2331 2331
Size: 6451
SalI G'TCGA_C
Cuts at: 1936 3793 1936
Size: 1857 4594
SanDI GG'GwC_CC
Cuts at: 2595 2595
Size: 6451
SbfI CC_TGCA'GG
Cuts at: 3803 3803
Size: 6451
ScaI AGT'ACT
Cuts at: 4699 6411 4699
Size: 1712 4739
SfcI C'TryA_G
Cuts at: 826 1045 3380 3799 4934 5615 5806 826
Size: 219 2335 419 1135 681 191 1471
Fragments arranged by size:
2335 1471 1135 681 419 219 191
SfoI GGC'GCC
Cuts at: 2190 2355 2380 3450 2190
Size: 165 25 1070 5191
Fragments arranged by size:
5191 1070 165 25
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6451
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6451
SmaI CCC'GGG
Cuts at: 1872 2653 3778 1872
Size: 781 1125 4545
Fragments arranged by size:
4545 1125 781
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2900 3740 4555 5456 5718 820
Size: 197 926 957 840 815 901 262 1553
Fragments arranged by size:
1553 957 926 901 840 815 262 197
SnaBI TAC'GTA
Cuts at: 493 4242 493
Size: 3749 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6451
SphI G_CATG'C
Cuts at: 1132 3809 1132
Size: 2677 3774
SspI AAT'ATT
Cuts at: 5 52 4375 5
Size: 47 4323 2081
Fragments arranged by size:
4323 2081 47
StuI AGG'CCT
Cuts at: 1517 2984 1517
Size: 1467 4984
StyI C'CwwG_G
Cuts at: 513 1065 3705 3890 513
Size: 552 2640 185 3074
Fragments arranged by size:
3074 2640 552 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2494 2599 3162 4478 4495 4648 4833
Size: 1255 914 105 563 1316 17 153 185
Cuts at: 4833 5278 325
Size: 445 1498
Fragments arranged by size:
1498 1316 1255 914 563 445 185 153
105 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4697 6409 96
Size: 274 80 33 51 1443 2720 1712 138
Fragments arranged by size:
2720 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2428 2460 3149 3181 3503 3535 6411 6443 2428
Size: 32 689 32 322 32 2876 32 2436
Fragments arranged by size:
2876 2436 689 322 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6451
XbaI T'CTAG_A
Cuts at: 3787 3787
Size: 6451
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6451
XmaI C'CCGG_G
Cuts at: 1870 2651 3776 1870
Size: 781 1125 4545
Fragments arranged by size:
4545 1125 781
XmnI GAAnn'nnTTC
Cuts at: 4580 4580
Size: 6451
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2769 4259 276
Size: 53 83 186 936 1235 1490 2468
Fragments arranged by size:
2468 1490 1235 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I Bsu36I EcoNI EcoRV FalI FspAI NruI NsiI
PacI PspXI PsrI RsrII SapI SexAI SfiI SrfI
SwaI XcmI