Restriction map for
pFN21AB8387
(Circular) (Six-base) MAPSORT of: pFN21AB8387.seq Check: 216 from: 1 to: 6409
pFN21AB8387 6409 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4219 278
Size: 53 83 186 936 2683 2468
Fragments arranged by size:
2683 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2505 3752 1937
Size: 568 1247 4594
Fragments arranged by size:
4594 1247 568
Acc65I G'GTAC_C
Cuts at: 2180 2982 3730 2180
Size: 802 748 4859
Fragments arranged by size:
4859 802 748
AclI AA'CG_TT
Cuts at: 1563 4536 4909 1563
Size: 2973 373 3063
Fragments arranged by size:
3063 2973 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2577 3090 3597 3657 3699 4472 5856 6124
Size: 945 513 507 60 42 773 1384 268
Cuts at: 6124 1632
Size: 1917
Fragments arranged by size:
1917 1384 945 773 513 507 268 60
42
AflII C'TTAA_G
Cuts at: 820 1017 2312 2351 820
Size: 197 1295 39 4878
Fragments arranged by size:
4878 1295 197 39
AflIII A'CryG_T
Cuts at: 1203 3688 5308 6285 1203
Size: 2485 1620 977 1327
Fragments arranged by size:
2485 1620 1327 977
AgeI A'CCGG_T
Cuts at: 2786 5240 2786
Size: 2454 3955
AhdI GACnn_n'nnGTC
Cuts at: 5138 5138
Size: 6409
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6409
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3247 1184
Size: 2063 4346
AlwNI CAG_nnn'CTG
Cuts at: 4152 5724 4152
Size: 1572 4837
ApaLI G'TGCA_C
Cuts at: 4466 5622 4466
Size: 1156 5253
ApoI r'AATT_y
Cuts at: 1493 2122 2765 3555 3718 3944 4096 6205 1493
Size: 629 643 790 163 226 152 2109 1697
Fragments arranged by size:
2109 1697 790 643 629 226 163 152
AseI AT'TA_AT
Cuts at: 160 1032 4963 160
Size: 872 3931 1606
Fragments arranged by size:
3931 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6409
AvaI C'yCGr_G
Cuts at: 1870 1943 3117 3734 1870
Size: 73 1174 617 4545
Fragments arranged by size:
4545 1174 617 73
AvrII C'CTAG_G
Cuts at: 3277 3277
Size: 6409
BamHI G'GATC_C
Cuts at: 3739 4106 3739
Size: 367 6042
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2180 2982 3296 3730 5185
Size: 325 217 627 393 802 314 434 1455
Cuts at: 5185 618
Size: 1842
Fragments arranged by size:
1842 1455 802 627 434 393 325 314
217
BanII G_rGCy'C
Cuts at: 1391 3728 1391
Size: 2337 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5438
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4600 4634 1903
Size: 34 2663 34 3678
Fragments arranged by size:
3678 2663 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3187 4301 5511 3187
Size: 1114 1210 4085
Fragments arranged by size:
4085 1210 1114
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6409
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2030 5020 136
Size: 107 122 71 631 605 358 2990 1525
Fragments arranged by size:
2990 1525 631 605 358 122 107 71
BglII A'GATC_T
Cuts at: 3448 6404 3448
Size: 2956 3453
BlpI GC'TnA_GC
Cuts at: 3826 3826
Size: 6409
Bme1580I G_kGCm'C
Cuts at: 1790 3299 4470 5626 1790
Size: 1509 1171 1156 2573
Fragments arranged by size:
2573 1509 1171 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6409
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4140 5098 442
Size: 416 53 83 399 24 2723 958 1753
Fragments arranged by size:
2723 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6409
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2421 2665 3422 5069 1239
Size: 1182 244 757 1647 2579
Fragments arranged by size:
2579 1647 1182 757 244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2530 3671 4534 5399 5697 2530
Size: 1141 863 865 298 3242
Fragments arranged by size:
3242 1141 865 863 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3319 3581 5072 882
Size: 617 1820 262 1491 2219
Fragments arranged by size:
2219 1820 1491 617 262
BsaAI yAC'GTr
Cuts at: 493 1204 4200 493
Size: 711 2996 2702
Fragments arranged by size:
2996 2702 711
BsaBI GATnn'nnATC
Cuts at: 6403 6403
Size: 6409
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4216 4598 5233 275
Size: 53 83 186 936 2683 382 635 1451
Fragments arranged by size:
2683 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2786 4842 5240 5514 5661 6072 2786
Size: 2056 398 274 147 411 3123
Fragments arranged by size:
3123 2056 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3624 3654 1178
Size: 30 165 30 2221 30 3933
Fragments arranged by size:
3933 2221 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6409
BseYI C'CCAG_C
Cuts at: 2099 5612 6108 2099
Size: 3513 496 2400
Fragments arranged by size:
3513 2400 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2988 1163
Size: 573 1252 4584
Fragments arranged by size:
4584 1252 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2107 3859 4620 5648 1540
Size: 275 186 106 1752 761 1028 2301
Fragments arranged by size:
2301 1752 1028 761 275 186 106
BsiHKAI G_wGCw'C
Cuts at: 4470 4555 5626 4470
Size: 85 1071 5253
Fragments arranged by size:
5253 1071 85
BsiWI C'GTAC_G
Cuts at: 2901 2901
Size: 6409
BsmI GAATG_Cn'
Cuts at: 3118 3320 3920 4013 3118
Size: 202 600 93 5514
Fragments arranged by size:
5514 600 202 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3299 3728 4470 4555 5626 1391
Size: 399 1509 429 742 85 1071 2174
Fragments arranged by size:
2174 1509 1071 742 429 399 85
BspEI T'CCGG_A
Cuts at: 6072 6072
Size: 6409
BspHI T'CATG_A
Cuts at: 2958 3430 4298 6028 2958
Size: 472 868 1730 3339
Fragments arranged by size:
3339 1730 868 472
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3764 844
Size: 2920 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4296 1394
Size: 429 2473 3507
Fragments arranged by size:
3507 2473 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4904 5078 65
Size: 1183 3656 174 1396
Fragments arranged by size:
3656 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2061 2786 5053 5240 1700
Size: 110 251 725 2267 187 2869
Fragments arranged by size:
2869 2267 725 251 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6409
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6409
BssSI C'ACGA_G
Cuts at: 4469 5481 4469
Size: 1012 5397
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6409
BstBI TT'CG_AA
Cuts at: 2455 5213 2455
Size: 2758 3651
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6409
BstXI CCAn_nnnn'nTGG
Cuts at: 3235 3451 3235
Size: 216 6193
BstYI r'GATC_y
Cuts at: 1972 2887 3448 3739 4106 4491 4508 5960 6046
Size: 915 561 291 367 385 17 1452 86
Cuts at: 6046 6058 6404 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 915 561 385 367 346 291
86 17 12
BstZ17I GTA'TAC
Cuts at: 2506 2506
Size: 6409
BtgI C'CryG_G
Cuts at: 513 1065 2028 2986 513
Size: 552 963 958 3936
Fragments arranged by size:
3936 963 958 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2089 487
Size: 683 56 863 4807
Fragments arranged by size:
4807 863 683 56
BtsI GCAGTG_nn'
Cuts at: 3925 4719 4739 3925
Size: 794 20 5595
Fragments arranged by size:
5595 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6409
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6374
DraI TTT'AAA
Cuts at: 2764 3713 2764
Size: 949 5460
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2472 5416 809
Size: 507 1156 2944 1802
Fragments arranged by size:
2944 1802 1156 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 3856 4745
Size: 54 1425 50 275 18 189 1837 889
Cuts at: 4745 6374 8
Size: 1629 43
Fragments arranged by size:
1837 1629 1425 889 275 189 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3856 1537
Size: 275 2044 4090
Fragments arranged by size:
4090 2044 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4339 1353
Size: 2986 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4996 5370 5516 387
Size: 1064 138 3407 374 146 1280
Fragments arranged by size:
3407 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2421 2577 2665 3090 3422 3597 3657
Size: 393 789 156 88 425 332 175 60
Cuts at: 3657 3699 4472 5069 5856 6124 1239
Size: 42 773 597 787 268 1524
Fragments arranged by size:
1524 789 787 773 597 425 393 332
268 175 156 88 60 42
EcoRI G'AATT_C
Cuts at: 3718 4096 3718
Size: 378 6031
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2327 2366 2327
Size: 39 6370
FspI TGC'GCA
Cuts at: 4915 4915
Size: 6409
HaeII r_GCGC'y
Cuts at: 1678 5556 1678
Size: 3878 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2354 2386 2477 2509 5050
Size: 32 135 32 782 32 91 32 2541
Cuts at: 5050 5082 5124 5156 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2541 782 135 91 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3753 3999 669
Size: 988 281 1815 246 3079
Fragments arranged by size:
3079 1815 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2140 748
Size: 1392 5017
HpaI GTT'AAC
Cuts at: 3999 3999
Size: 6409
KpnI G_GTAC'C
Cuts at: 2184 2986 3734 2184
Size: 802 748 4859
Fragments arranged by size:
4859 802 748
MfeI C'AATT_G
Cuts at: 4008 4008
Size: 6409
MluI A'CGCG_T
Cuts at: 6285 6285
Size: 6409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5523 5707 1305
Size: 4218 184 2007
Fragments arranged by size:
4218 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4587
Fragments arranged by size:
4587 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3233 4367 4726 4885 518
Size: 648 2067 1134 359 159 2042
Fragments arranged by size:
2067 2042 1134 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2030 3825 4502 5650 5895 1606
Size: 424 1795 677 1148 245 2120
Fragments arranged by size:
2120 1795 1148 677 424 245
NaeI GCC'GGC
Cuts at: 2063 2063
Size: 6409
NcoI C'CATG_G
Cuts at: 513 1065 2986 513
Size: 552 1921 3936
Fragments arranged by size:
3936 1921 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6409
NgoMIV G'CCGG_C
Cuts at: 2061 2061
Size: 6409
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6409
NotI GC'GGCC_GC
Cuts at: 3856 3856
Size: 6409
NspI r_CATG'y
Cuts at: 1132 1229 3692 3767 5312 1132
Size: 97 2463 75 1545 2229
Fragments arranged by size:
2463 2229 1545 97 75
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6409
PciI A'CATG_T
Cuts at: 3688 5308 3688
Size: 1620 4789
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2177 1108
Size: 1069 5340
PfoI T'CCnGG_A
Cuts at: 3399 3399
Size: 6409
PmeI GTTT'AAAC
Cuts at: 3713 3713
Size: 6409
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4478 4510 5991 6023 4478
Size: 32 1481 32 4864
Fragments arranged by size:
4864 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6409
PsiI TTA'TAA
Cuts at: 3218 3979 3218
Size: 761 5648
PstI C_TGCA'G
Cuts at: 830 3205 3761 830
Size: 2375 556 3478
Fragments arranged by size:
3478 2375 556
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6409
SacII CC_GC'GG
Cuts at: 2031 2031
Size: 6409
SalI G'TCGA_C
Cuts at: 1936 3751 1936
Size: 1815 4594
SbfI CC_TGCA'GG
Cuts at: 3761 3761
Size: 6409
ScaI AGT'ACT
Cuts at: 4657 6369 4657
Size: 1712 4697
SfcI C'TryA_G
Cuts at: 826 1045 2161 3201 3757 4892 5573 5764 826
Size: 219 1116 1040 556 1135 681 191 1471
Fragments arranged by size:
1471 1135 1116 1040 681 556 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6409
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6409
SmaI CCC'GGG
Cuts at: 1872 3736 1872
Size: 1864 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2312 2351 2509 3650 4513 5414
Size: 197 926 369 39 158 1141 863 901
Cuts at: 5414 5676 820
Size: 262 1553
Fragments arranged by size:
1553 1141 926 901 863 369 262 197
158 39
SnaBI TAC'GTA
Cuts at: 493 4200 493
Size: 3707 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6409
SphI G_CATG'C
Cuts at: 1132 3767 1132
Size: 2635 3774
SspI AAT'ATT
Cuts at: 5 52 2637 3616 4333 5
Size: 47 2585 979 717 2081
Fragments arranged by size:
2585 2081 979 717 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6409
StyI C'CwwG_G
Cuts at: 513 1065 2986 3277 3848 513
Size: 552 1921 291 571 3074
Fragments arranged by size:
3074 1921 571 552 291
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2954 3278 4436 4453 4606 4791 5236
Size: 1255 1374 324 1158 17 153 185 445
Cuts at: 5236 325
Size: 1498
Fragments arranged by size:
1498 1374 1255 1158 445 324 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2202 2376 3170
Size: 274 80 33 51 1443 225 174 794
Cuts at: 3170 3379 4655 6367 96
Size: 209 1276 1712 138
Fragments arranged by size:
1712 1443 1276 794 274 225 209 174
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2706 2738 6369 6401 2706
Size: 32 3631 32 2714
Fragments arranged by size:
3631 2714 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2472 1316
Size: 1156 5253
XbaI T'CTAG_A
Cuts at: 2443 3745 2443
Size: 1302 5107
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6409
XmaI C'CCGG_G
Cuts at: 1870 3734 1870
Size: 1864 4545
XmnI GAAnn'nnTTC
Cuts at: 2143 2390 2451 3027 4538 2143
Size: 247 61 576 1511 4014
Fragments arranged by size:
4014 1511 576 247 61
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4217 276
Size: 53 83 186 936 2683 2468
Fragments arranged by size:
2683 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SacII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsmBI Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI