Restriction map for
pFN21AB8385
(Circular) (Six-base) MAPSORT of: pFN21AB8385.seq Check: 5007 from: 1 to: 5974
pFN21AB8385 5974 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2318 2908 2318
Size: 590 5384
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3784 278
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2946 3317 1937
Size: 1009 371 4594
Fragments arranged by size:
4594 1009 371
Acc65I G'GTAC_C
Cuts at: 3295 3295
Size: 5974
AclI AA'CG_TT
Cuts at: 1563 4101 4474 1563
Size: 2538 373 3063
Fragments arranged by size:
3063 2538 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2671 4037 5421 5689 1632
Size: 1039 1366 1384 268 1917
Fragments arranged by size:
1917 1384 1366 1039 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5777
AflIII A'CryG_T
Cuts at: 1203 2941 3255 4873 5850 1203
Size: 1738 314 1618 977 1327
Fragments arranged by size:
1738 1618 1327 977 314
AgeI A'CCGG_T
Cuts at: 4805 4805
Size: 5974
AhdI GACnn_n'nnGTC
Cuts at: 2749 4703 2749
Size: 1954 4020
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5974
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3131 1184
Size: 1947 4027
AlwNI CAG_nnn'CTG
Cuts at: 2385 3717 5289 2385
Size: 1332 1572 3070
Fragments arranged by size:
3070 1572 1332
ApaI G_GGCC'C
Cuts at: 2225 2707 2225
Size: 482 5492
ApaLI G'TGCA_C
Cuts at: 4031 5187 4031
Size: 1156 4818
ApoI r'AATT_y
Cuts at: 1493 3283 3509 3661 5770 1493
Size: 1790 226 152 2109 1697
Fragments arranged by size:
2109 1790 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2145 2145
Size: 5974
AseI AT'TA_AT
Cuts at: 160 1032 4528 160
Size: 872 3496 1606
Fragments arranged by size:
3496 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
AvaI C'yCGr_G
Cuts at: 1870 1943 2140 2167 3299 1870
Size: 73 197 27 1132 4545
Fragments arranged by size:
4545 1132 197 73 27
BamHI G'GATC_C
Cuts at: 3304 3671 3304
Size: 367 5607
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2111 2824 3295 4750 618
Size: 325 217 627 324 713 471 1455 1842
Fragments arranged by size:
1842 1455 713 627 471 325 324 217
BanII G_rGCy'C
Cuts at: 1391 2141 2225 2595 2707 3293 1391
Size: 750 84 370 112 586 4072
Fragments arranged by size:
4072 750 586 370 112 84
BbeI G_GCGC'C
Cuts at: 2115 2828 2115
Size: 713 5261
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5003
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4165 4199 1903
Size: 34 2228 34 3678
Fragments arranged by size:
3678 2228 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3866 5076 3866
Size: 1210 4764
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5974
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3105 4585 136
Size: 107 122 71 631 605 1433 1480 1525
Fragments arranged by size:
1525 1480 1433 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5969 5969
Size: 5974
BlpI GC'TnA_GC
Cuts at: 3391 3391
Size: 5974
Bme1580I G_kGCm'C
Cuts at: 1790 2225 2707 4035 5191 1790
Size: 435 482 1328 1156 2573
Fragments arranged by size:
2573 1328 1156 482 435
BmgBI CAC'GTC
Cuts at: 1318 2047 2604 2944 2991 1318
Size: 729 557 340 47 4301
Fragments arranged by size:
4301 729 557 340 47
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2794 3705 4663
Size: 416 53 83 399 24 1377 911 958
Cuts at: 4663 442
Size: 1753
Fragments arranged by size:
1753 1377 958 911 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2791 3080 4634 1239
Size: 1552 289 1554 2579
Fragments arranged by size:
2579 1554 1552 289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2417 2915 4099 4964 5262 2417
Size: 498 1184 865 298 3129
Fragments arranged by size:
3129 1184 865 498 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4637 882
Size: 617 3138 2219
Fragments arranged by size:
3138 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2821 3070 3765 493
Size: 711 1617 249 695 2702
Fragments arranged by size:
2702 1617 711 695 249
BsaBI GATnn'nnATC
Cuts at: 5968 5968
Size: 5974
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2112 2825 2997 3781
Size: 53 83 186 936 579 713 172 784
Cuts at: 3781 4163 4798 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 784 713 635 579 382 186
172 83 53
BsaWI w'CCGG_w
Cuts at: 4407 4805 5079 5226 5637 4407
Size: 398 274 147 411 4744
Fragments arranged by size:
4744 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5749
Fragments arranged by size:
5749 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2353 3077 3088 3139 1186
Size: 1167 724 11 51 4021
Fragments arranged by size:
4021 1167 724 51 11
BseYI C'CCAG_C
Cuts at: 2496 3095 5177 5673 2496
Size: 599 2082 496 2797
Fragments arranged by size:
2797 2082 599 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2801 1163
Size: 573 1065 4336
Fragments arranged by size:
4336 1065 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2875 3424 4185 5213 1540
Size: 275 186 874 549 761 1028 2301
Fragments arranged by size:
2301 1028 874 761 549 275 186
BsiHKAI G_wGCw'C
Cuts at: 2790 2807 3272 4035 4120 5191 2790
Size: 17 465 763 85 1071 3573
Fragments arranged by size:
3573 1071 763 465 85 17
BsmI GAATG_Cn'
Cuts at: 2490 3485 3578 2490
Size: 995 93 4886
Fragments arranged by size:
4886 995 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2141 2225 2595 2707 2790 2807 3272
Size: 399 351 84 370 112 83 17 465
Cuts at: 3272 3293 4035 4120 5191 1391
Size: 21 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 465 399 370 351 112
85 84 83 21 17
BspEI T'CCGG_A
Cuts at: 5637 5637
Size: 5974
BspHI T'CATG_A
Cuts at: 3863 5593 3863
Size: 1730 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2318 2908 3329 844
Size: 1474 590 421 3489
Fragments arranged by size:
3489 1474 590 421
BsrBI CCG'CTC
Cuts at: 1394 1823 2286 3861 1394
Size: 429 463 1575 3507
Fragments arranged by size:
3507 1575 463 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2016 4469 4643 65
Size: 1183 768 2453 174 1396
Fragments arranged by size:
2453 1396 1183 768 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2114 2843 4618 4805 1700
Size: 110 304 729 1775 187 2869
Fragments arranged by size:
2869 1775 729 304 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5974
BssHII G'CGCG_C
Cuts at: 1925 2145 2584 2675 1925
Size: 220 439 91 5224
Fragments arranged by size:
5224 439 220 91
BssSI C'ACGA_G
Cuts at: 4034 5046 4034
Size: 1012 4962
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2415 2622 794
Size: 1621 207 4146
Fragments arranged by size:
4146 1621 207
BstBI TT'CG_AA
Cuts at: 4778 4778
Size: 5974
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5974
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5974
BstYI r'GATC_y
Cuts at: 1972 2032 2728 3304 3671 4056 4073 5525 5611
Size: 60 696 576 367 385 17 1452 86
Cuts at: 5611 5623 5969 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 696 576 385 367 346 86
60 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2095 513
Size: 552 939 91 4392
Fragments arranged by size:
4392 939 552 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5235
Fragments arranged by size:
5235 683 56
BtsI GCAGTG_nn'
Cuts at: 2548 3056 3490 4284 4304 2548
Size: 508 434 794 20 4218
Fragments arranged by size:
4218 794 508 434 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5974
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5939
DraI TTT'AAA
Cuts at: 3278 3278
Size: 5974
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4981 809
Size: 507 3665 1802
Fragments arranged by size:
3665 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2299 2545
Size: 54 1425 50 275 18 219 250 246
Cuts at: 2545 2670 2835 3421 4310 5939 8
Size: 125 165 586 889 1629 43
Fragments arranged by size:
1629 1425 889 586 275 250 246 219
165 125 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3421 1537
Size: 275 1609 4090
Fragments arranged by size:
4090 1609 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2690 3173 3904 1353
Size: 1337 483 731 3423
Fragments arranged by size:
3423 1337 731 483
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4561 4935 5081 387
Size: 1064 138 2972 374 146 1280
Fragments arranged by size:
2972 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2671 2791 3080 4037 4634 5421 5689
Size: 393 1039 120 289 957 597 787 268
Cuts at: 5689 1239
Size: 1524
Fragments arranged by size:
1524 1039 957 787 597 393 289 268
120
EcoO109I rG'GnC_Cy
Cuts at: 2221 2533 2221
Size: 312 5662
EcoRI G'AATT_C
Cuts at: 3283 3661 3283
Size: 378 5596
FspI TGC'GCA
Cuts at: 3055 3137 4480 3055
Size: 82 1343 4549
Fragments arranged by size:
4549 1343 82
FspAI rTGC'GCAy
Cuts at: 3055 3055
Size: 5974
HaeII r_GCGC'y
Cuts at: 1678 2115 2186 2694 2828 2939 5121 1678
Size: 437 71 508 134 111 2182 2531
Fragments arranged by size:
2531 2182 508 437 134 111 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2735 2767 4615 4647 4689
Size: 32 135 32 1163 32 1848 32 42
Cuts at: 4689 4721 1373
Size: 32 2626
Fragments arranged by size:
2626 1848 1163 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2947 3318 3564 669
Size: 988 281 1009 371 246 3079
Fragments arranged by size:
3079 1009 988 371 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5974
HpaI GTT'AAC
Cuts at: 3564 3564
Size: 5974
KasI G'GCGC_C
Cuts at: 2111 2824 2111
Size: 713 5261
KpnI G_GTAC'C
Cuts at: 3299 3299
Size: 5974
MfeI C'AATT_G
Cuts at: 3573 3573
Size: 5974
MluI A'CGCG_T
Cuts at: 5850 5850
Size: 5974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2516 2991 5088 5272 1305
Size: 1211 475 2097 184 2007
Fragments arranged by size:
2097 2007 1211 475 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4152
Fragments arranged by size:
4152 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3932 4291 4450 518
Size: 648 843 1923 359 159 2042
Fragments arranged by size:
2042 1923 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2025 2097 2815 2964 3390 4067 5215 5460
Size: 419 72 718 149 426 677 1148 245
Cuts at: 5460 1606
Size: 2120
Fragments arranged by size:
2120 1148 718 677 426 419 245 149
72
NaeI GCC'GGC
Cuts at: 2116 2845 2116
Size: 729 5245
NarI GG'CG_CC
Cuts at: 2112 2825 2112
Size: 713 5261
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4483
Fragments arranged by size:
4483 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5974
NgoMIV G'CCGG_C
Cuts at: 2114 2843 2114
Size: 729 5245
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5974
NotI GC'GGCC_GC
Cuts at: 3421 3421
Size: 5974
NspI r_CATG'y
Cuts at: 1132 1229 2370 2629 2855 3259 3332 4877 1132
Size: 97 1141 259 226 404 73 1545 2229
Fragments arranged by size:
2229 1545 1141 404 259 226 97 73
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5974
PciI A'CATG_T
Cuts at: 3255 4873 3255
Size: 1618 4356
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5974
PfoI T'CCnGG_A
Cuts at: 2724 2910 2724
Size: 186 5788
PmeI GTTT'AAAC
Cuts at: 3278 3278
Size: 5974
PmlI CAC'GTG
Cuts at: 2821 2821
Size: 5974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4043 4075 5556 5588 4043
Size: 32 1481 32 4429
Fragments arranged by size:
4429 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2533 2533
Size: 5974
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5974
PsiI TTA'TAA
Cuts at: 3544 3544
Size: 5974
PspOMI G'GGCC_C
Cuts at: 2221 2703 2221
Size: 482 5492
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2345 2377 2345
Size: 32 5942
PstI C_TGCA'G
Cuts at: 830 2736 3039 3326 830
Size: 1906 303 287 3478
Fragments arranged by size:
3478 1906 303 287
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5974
PvuII CAG'CTG
Cuts at: 2025 2964 2025
Size: 939 5035
SacII CC_GC'GG
Cuts at: 2098 2098
Size: 5974
SalI G'TCGA_C
Cuts at: 1936 2945 3316 1936
Size: 1009 371 4594
Fragments arranged by size:
4594 1009 371
SanDI GG'GwC_CC
Cuts at: 2533 2533
Size: 5974
SapI GCTCTTCn'nnn_
Cuts at: 2690 3173 2690
Size: 483 5491
SbfI CC_TGCA'GG
Cuts at: 3039 3326 3039
Size: 287 5687
ScaI AGT'ACT
Cuts at: 4222 5934 4222
Size: 1712 4262
SexAI A'CCwGG_T
Cuts at: 2745 2745
Size: 5974
SfcI C'TryA_G
Cuts at: 826 1045 2732 3035 3322 4457 5138 5329 826
Size: 219 1687 303 287 1135 681 191 1471
Fragments arranged by size:
1687 1471 1135 681 303 287 219 191
SfoI GGC'GCC
Cuts at: 2113 2826 2113
Size: 713 5261
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
SgrAI Cr'CCGG_yG
Cuts at: 1700 2114 1700
Size: 414 5560
SmaI CCC'GGG
Cuts at: 1872 2169 3301 1872
Size: 297 1132 4545
Fragments arranged by size:
4545 1132 297
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2432 2930 4078 4979 5241 820
Size: 197 926 489 498 1148 901 262 1553
Fragments arranged by size:
1553 1148 926 901 498 489 262 197
SnaBI TAC'GTA
Cuts at: 493 3765 493
Size: 3272 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5974
SphI G_CATG'C
Cuts at: 1132 2629 2855 3332 1132
Size: 1497 226 477 3774
Fragments arranged by size:
3774 1497 477 226
SspI AAT'ATT
Cuts at: 5 52 3898 5
Size: 47 3846 2081
Fragments arranged by size:
3846 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5974
StyI C'CwwG_G
Cuts at: 513 1065 2004 3413 513
Size: 552 939 1409 3074
Fragments arranged by size:
3074 1409 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4001 4018 4171 4356 4801 325
Size: 1255 2421 17 153 185 445 1498
Fragments arranged by size:
2421 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2958 4220 5932
Size: 274 80 33 51 1443 981 1262 1712
Cuts at: 5932 96
Size: 138
Fragments arranged by size:
1712 1443 1262 981 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5934 5966 5934
Size: 32 5942
Tth111I GACn'n_nGTC
Cuts at: 1316 2876 1316
Size: 1560 4414
XbaI T'CTAG_A
Cuts at: 3310 3310
Size: 5974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2641 2641
Size: 5974
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5974
XmaI C'CCGG_G
Cuts at: 1870 2167 3299 1870
Size: 297 1132 4545
Fragments arranged by size:
4545 1132 297
XmnI GAAnn'nnTTC
Cuts at: 2469 4103 2469
Size: 1634 4340
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3782 276
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI NruI NsiI PacI PspXI RsrII SacI
SfiI SrfI SwaI