Restriction map for
pFN21AB8384
(Circular) (Six-base) MAPSORT of: pFN21AB8384.seq Check: 5786 from: 1 to: 6553
pFN21AB8384 6553 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4363 278
Size: 53 83 186 936 2827 2468
Fragments arranged by size:
2827 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3759 3896 1937
Size: 1822 137 4594
Fragments arranged by size:
4594 1822 137
Acc65I G'GTAC_C
Cuts at: 2111 3874 2111
Size: 1763 4790
AclI AA'CG_TT
Cuts at: 1563 4680 5053 1563
Size: 3117 373 3063
Fragments arranged by size:
3117 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2081 2186 2647 2781 3057 3327 4616 6000
Size: 449 105 461 134 276 270 1289 1384
Cuts at: 6000 6268 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1289 461 449 276 270 268
134 105
AflII C'TTAA_G
Cuts at: 820 1017 2741 820
Size: 197 1724 4632
Fragments arranged by size:
4632 1724 197
AflIII A'CryG_T
Cuts at: 1203 2271 5452 6429 1203
Size: 1068 3181 977 1327
Fragments arranged by size:
3181 1327 1068 977
AgeI A'CCGG_T
Cuts at: 5384 5384
Size: 6553
AhdI GACnn_n'nnGTC
Cuts at: 5282 5282
Size: 6553
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6553
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2988 1184
Size: 1804 4749
AlwNI CAG_nnn'CTG
Cuts at: 3134 3467 4296 5868 3134
Size: 333 829 1572 3819
Fragments arranged by size:
3819 1572 829 333
ApaI G_GGCC'C
Cuts at: 2056 2573 3013 3641 2056
Size: 517 440 628 4968
Fragments arranged by size:
4968 628 517 440
ApaLI G'TGCA_C
Cuts at: 4610 5766 4610
Size: 1156 5397
ApoI r'AATT_y
Cuts at: 1493 2168 3442 3675 3862 4088 4240 6349 1493
Size: 675 1274 233 187 226 152 2109 1697
Fragments arranged by size:
2109 1697 1274 675 233 226 187 152
AseI AT'TA_AT
Cuts at: 160 1032 5107 160
Size: 872 4075 1606
Fragments arranged by size:
4075 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6553
AvaI C'yCGr_G
Cuts at: 1870 1943 2592 2781 3006 3231 3878 1870
Size: 73 649 189 225 225 647 4545
Fragments arranged by size:
4545 649 647 225 225 189 73
BamHI G'GATC_C
Cuts at: 2148 2289 2646 3883 4250 2148
Size: 141 357 1237 367 4451
Fragments arranged by size:
4451 1237 367 357 141
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2111 3077 3121 3405 3874
Size: 325 217 627 324 966 44 284 469
Cuts at: 3874 5329 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 966 627 469 325 324 284
217 44
BanII G_rGCy'C
Cuts at: 1391 2036 2056 2573 2836 2942 3013 3208 3518
Size: 645 20 517 263 106 71 195 310
Cuts at: 3518 3641 3872 1391
Size: 123 231 4072
Fragments arranged by size:
4072 645 517 310 263 231 195 123
106 71 20
BbeI G_GCGC'C
Cuts at: 3409 3409
Size: 6553
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2510 928
Size: 971 611 4971
Fragments arranged by size:
4971 971 611
BbvCI CC'TCA_GC
Cuts at: 2070 2070
Size: 6553
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4744 4778 1903
Size: 34 2807 34 3678
Fragments arranged by size:
3678 2807 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3367 4445 5655 3367
Size: 1078 1210 4265
Fragments arranged by size:
4265 1210 1078
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6553
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2981 5164 136
Size: 107 122 71 631 605 1309 2183 1525
Fragments arranged by size:
2183 1525 1309 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2427 6548 2427
Size: 4121 2432
BlpI GC'TnA_GC
Cuts at: 3970 3970
Size: 6553
Bme1580I G_kGCm'C
Cuts at: 1790 2056 2573 2623 3013 3082 3124 3141 3239
Size: 266 517 50 390 69 42 17 98
Cuts at: 3239 3641 4614 5770 1790
Size: 402 973 1156 2573
Fragments arranged by size:
2573 1156 973 517 402 390 266 98
69 50 42 17
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6553
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2052 2644 2898
Size: 416 53 83 399 24 635 592 254
Cuts at: 2898 3032 3478 3845 4284 5242 442
Size: 134 446 367 439 958 1753
Fragments arranged by size:
1753 958 635 592 446 439 416 399
367 254 134 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6553
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2247 3561 5213 1239
Size: 1008 1314 1652 2579
Fragments arranged by size:
2579 1652 1314 1008
Bpu10I CC'TnA_GC
Cuts at: 2070 3517 2070
Size: 1447 5106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3440 3475 4678 5543 5841 3440
Size: 35 1203 865 298 4152
Fragments arranged by size:
4152 1203 865 298 35
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5216 882
Size: 617 3717 2219
Fragments arranged by size:
3717 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2909 4344 493
Size: 711 1705 1435 2702
Fragments arranged by size:
2702 1705 1435 711
BsaBI GATnn'nnATC
Cuts at: 2352 6547 2352
Size: 4195 2358
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3406 4360 4742 5377
Size: 53 83 186 936 1873 954 382 635
Cuts at: 5377 275
Size: 1451
Fragments arranged by size:
1873 1451 954 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4986 5384 5658 5805 6216 4986
Size: 398 274 147 411 5323
Fragments arranged by size:
5323 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2258 2288 2600 2630 1178
Size: 30 165 30 855 30 312 30 5101
Fragments arranged by size:
5101 855 312 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2279 1186
Size: 1093 5460
BseYI C'CCAG_C
Cuts at: 2075 2584 2859 3127 3648 5756 6252 2075
Size: 509 275 268 521 2108 496 2376
Fragments arranged by size:
2376 2108 521 509 496 275 268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2150 2709 3750 1163
Size: 573 414 559 1041 3966
Fragments arranged by size:
3966 1041 573 559 414
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4003 4764 5792 1540
Size: 275 186 2002 761 1028 2301
Fragments arranged by size:
2301 2002 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2313 2942 3208 4614 4699 5770 2313
Size: 629 266 1406 85 1071 3096
Fragments arranged by size:
3096 1406 1071 629 266 85
BsmI GAATG_Cn'
Cuts at: 4064 4157 4064
Size: 93 6460
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2056 2313 2573 2623 2836 2942
Size: 399 246 20 257 260 50 213 106
Cuts at: 2942 3013 3082 3124 3141 3208 3239 3518 3641
Size: 71 69 42 17 67 31 279 123
Cuts at: 3641 3872 4614 4699 5770 1391
Size: 231 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 279 260 257 246
231 213 123 106 85 71 69 67
50 42 31 20 17
BspEI T'CCGG_A
Cuts at: 6216 6216
Size: 6553
BspHI T'CATG_A
Cuts at: 4442 6172 4442
Size: 1730 4823
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2956 2970 3908 844
Size: 2112 14 938 3489
Fragments arranged by size:
3489 2112 938 14
BsrBI CCG'CTC
Cuts at: 1394 1823 2644 2917 4440 1394
Size: 429 821 273 1523 3507
Fragments arranged by size:
3507 1523 821 429 273
BsrDI GCAATG_nn'
Cuts at: 65 1248 2266 5048 5222 65
Size: 1183 1018 2782 174 1396
Fragments arranged by size:
2782 1396 1183 1018 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2894 5197 5384 1700
Size: 110 1084 2303 187 2869
Fragments arranged by size:
2869 2303 1084 187 110
BsrGI T'GTAC_A
Cuts at: 96 2161 96
Size: 2065 4488
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6553
BssSI C'ACGA_G
Cuts at: 4613 5625 4613
Size: 1012 5541
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2618 3386 3593 794
Size: 1824 768 207 3754
Fragments arranged by size:
3754 1824 768 207
BstBI TT'CG_AA
Cuts at: 5357 5357
Size: 6553
BstEII G'GTnAC_C
Cuts at: 1184 3266 3495 1184
Size: 2082 229 4242
Fragments arranged by size:
4242 2082 229
BstYI r'GATC_y
Cuts at: 1972 2148 2289 2427 2646 2872 3883 4250 4635
Size: 176 141 138 219 226 1011 367 385
Cuts at: 4635 4652 6104 6190 6202 6548 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1011 385 367 346 226 219
176 141 138 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2212 2868 2212
Size: 656 5897
BtgI C'CryG_G
Cuts at: 513 1065 2004 2748 3471 3500 513
Size: 552 939 744 723 29 3566
Fragments arranged by size:
3566 939 744 723 552 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5814
Fragments arranged by size:
5814 683 56
BtsI GCAGTG_nn'
Cuts at: 3095 3762 4069 4863 4883 3095
Size: 667 307 794 20 4765
Fragments arranged by size:
4765 794 667 307 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6553
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6518
DraI TTT'AAA
Cuts at: 3857 3857
Size: 6553
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2512 3290 5560 809
Size: 507 1196 778 2270 1802
Fragments arranged by size:
2270 1802 1196 778 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2461 4000 4889
Size: 54 1425 50 275 18 631 1539 889
Cuts at: 4889 6518 8
Size: 1629 43
Fragments arranged by size:
1629 1539 1425 889 631 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4000 1537
Size: 275 2188 4090
Fragments arranged by size:
4090 2188 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2301 2757 2918 4483 1353
Size: 948 456 161 1565 3423
Fragments arranged by size:
3423 1565 948 456 161
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3018 5140 5514 5660 387
Size: 1064 138 1429 2122 374 146 1280
Fragments arranged by size:
2122 1429 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2940 3206 2940
Size: 266 6287
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2186 2247 2647 2781 3057 3327
Size: 393 449 105 61 400 134 276 270
Cuts at: 3327 3561 4616 5213 6000 6268 1239
Size: 234 1055 597 787 268 1524
Fragments arranged by size:
1524 1055 787 597 449 400 393 276
270 268 234 134 105 61
EcoO109I rG'GnC_Cy
Cuts at: 2052 2053 2216 3010 3637 3638 2052
Size: 1 163 794 627 1 4967
Fragments arranged by size:
4967 794 627 163 1 1
EcoRI G'AATT_C
Cuts at: 3442 3862 4240 3442
Size: 420 378 5755
Fragments arranged by size:
5755 420 378
FspI TGC'GCA
Cuts at: 5059 5059
Size: 6553
HaeII r_GCGC'y
Cuts at: 1678 3409 5700 1678
Size: 1731 2291 2531
Fragments arranged by size:
2531 2291 1731
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2222 2254 5194 5226 5268
Size: 32 135 32 650 32 2940 32 42
Cuts at: 5268 5300 1373
Size: 32 2626
Fragments arranged by size:
2940 2626 650 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3897 4143 669
Size: 988 281 1959 246 3079
Fragments arranged by size:
3079 1959 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6553
HpaI GTT'AAC
Cuts at: 4143 4143
Size: 6553
KasI G'GCGC_C
Cuts at: 3405 3405
Size: 6553
KpnI G_GTAC'C
Cuts at: 2115 3878 2115
Size: 1763 4790
MfeI C'AATT_G
Cuts at: 4152 4152
Size: 6553
MluI A'CGCG_T
Cuts at: 6429 6429
Size: 6553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5667 5851 1305
Size: 4362 184 2007
Fragments arranged by size:
4362 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4731
Fragments arranged by size:
4731 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2733 3149 4511 4870 5029 518
Size: 648 1567 416 1362 359 159 2042
Fragments arranged by size:
2042 1567 1362 648 416 359 159
MspA1I CmG'CkG
Cuts at: 1606 3134 3305 3467 3539 3969 4646 5794 6039
Size: 1528 171 162 72 430 677 1148 245
Cuts at: 6039 1606
Size: 2120
Fragments arranged by size:
2120 1528 1148 677 430 245 171 162
72
NarI GG'CG_CC
Cuts at: 3406 3406
Size: 6553
NcoI C'CATG_G
Cuts at: 513 1065 2004 2748 3471 3500 513
Size: 552 939 744 723 29 3566
Fragments arranged by size:
3566 939 744 723 552 29
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6553
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6553
NotI GC'GGCC_GC
Cuts at: 4000 4000
Size: 6553
NspI r_CATG'y
Cuts at: 1132 1229 2275 2732 2777 3800 3911 5456 1132
Size: 97 1046 457 45 1023 111 1545 2229
Fragments arranged by size:
2229 1545 1046 1023 457 111 97 45
PasI CC'CwG_GG
Cuts at: 1348 3598 1348
Size: 2250 4303
PciI A'CATG_T
Cuts at: 2271 5452 2271
Size: 3181 3372
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2807 2828 1108
Size: 1699 21 4833
Fragments arranged by size:
4833 1699 21
PmeI GTTT'AAAC
Cuts at: 3857 3857
Size: 6553
PmlI CAC'GTG
Cuts at: 2909 2909
Size: 6553
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4622 4654 6135 6167 4622
Size: 32 1481 32 5008
Fragments arranged by size:
5008 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2216 2216
Size: 6553
PshAI GACnn'nnGTC
Cuts at: 1613 2322 1613
Size: 709 5844
PsiI TTA'TAA
Cuts at: 4123 4123
Size: 6553
PspOMI G'GGCC_C
Cuts at: 2052 2569 3009 3637 2052
Size: 517 440 628 4968
Fragments arranged by size:
4968 628 517 440
PstI C_TGCA'G
Cuts at: 830 2967 3905 830
Size: 2137 938 3478
Fragments arranged by size:
3478 2137 938
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6553
PvuII CAG'CTG
Cuts at: 3134 3305 3467 3539 3134
Size: 171 162 72 6148
Fragments arranged by size:
6148 171 162 72
SacI G_AGCT'C
Cuts at: 2942 3208 2942
Size: 266 6287
SalI G'TCGA_C
Cuts at: 1936 3895 1936
Size: 1959 4594
SapI GCTCTTCn'nnn_
Cuts at: 2301 2301
Size: 6553
SbfI CC_TGCA'GG
Cuts at: 2967 3905 2967
Size: 938 5615
ScaI AGT'ACT
Cuts at: 2363 3591 4801 6513 2363
Size: 1228 1210 1712 2403
Fragments arranged by size:
2403 1712 1228 1210
SfcI C'TryA_G
Cuts at: 826 1045 2281 2485 2696 2963 3901 5036 5717
Size: 219 1236 204 211 267 938 1135 681
Cuts at: 5717 5908 826
Size: 191 1471
Fragments arranged by size:
1471 1236 1135 938 681 267 219 211
204 191
SfoI GGC'GCC
Cuts at: 3407 3407
Size: 6553
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6553
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6553
SmaI CCC'GGG
Cuts at: 1872 3008 3233 3880 1872
Size: 1136 225 647 4545
Fragments arranged by size:
4545 1136 647 225
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2592 2741 3455 3490 4657 5558
Size: 197 926 649 149 714 35 1167 901
Cuts at: 5558 5820 820
Size: 262 1553
Fragments arranged by size:
1553 1167 926 901 714 649 262 197
149 35
SnaBI TAC'GTA
Cuts at: 493 4344 493
Size: 3851 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6553
SphI G_CATG'C
Cuts at: 1132 3911 1132
Size: 2779 3774
SspI AAT'ATT
Cuts at: 5 52 2480 4477 5
Size: 47 2428 1997 2081
Fragments arranged by size:
2428 2081 1997 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6553
StyI C'CwwG_G
Cuts at: 513 1065 2004 2121 2508 2748 3271 3471 3500
Size: 552 939 117 387 240 523 200 29
Cuts at: 3500 3681 3992 513
Size: 181 311 3074
Fragments arranged by size:
3074 939 552 523 387 311 240 200
181 117 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4580 4597 4750 4935 5380 325
Size: 1255 3000 17 153 185 445 1498
Fragments arranged by size:
3000 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2161 2249 2268
Size: 274 80 33 51 1443 184 88 19
Cuts at: 2268 2361 3589 4799 6511 96
Size: 93 1228 1210 1712 138
Fragments arranged by size:
1712 1443 1228 1210 274 184 138 93
88 80 51 33 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2350 2382 2600 2632 2911 2943 6513 6545 2350
Size: 32 218 32 279 32 3570 32 2358
Fragments arranged by size:
3570 2358 279 218 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6553
XbaI T'CTAG_A
Cuts at: 3889 3889
Size: 6553
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3507 3507
Size: 6553
XhoI C'TCGA_G
Cuts at: 1943 2592 1943
Size: 649 5904
XmaI C'CCGG_G
Cuts at: 1870 3006 3231 3878 1870
Size: 1136 225 647 4545
Fragments arranged by size:
4545 1136 647 225
XmnI GAAnn'nnTTC
Cuts at: 4682 4682
Size: 6553
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4361 276
Size: 53 83 186 936 2827 2468
Fragments arranged by size:
2827 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI
SwaI