Restriction map for
pFN21AB8383
(Circular) (Six-base) MAPSORT of: pFN21AB8383.seq Check: 6802 from: 1 to: 6442
pFN21AB8383 6442 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4252 278
Size: 53 83 186 936 2716 2468
Fragments arranged by size:
2716 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3785 1937
Size: 1848 4594
Acc65I G'GTAC_C
Cuts at: 3763 3763
Size: 6442
AclI AA'CG_TT
Cuts at: 1563 4569 4942 1563
Size: 3006 373 3063
Fragments arranged by size:
3063 3006 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4505 5889 6157 1632
Size: 2873 1384 268 1917
Fragments arranged by size:
2873 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6245
AflIII A'CryG_T
Cuts at: 1203 2265 5341 6318 1203
Size: 1062 3076 977 1327
Fragments arranged by size:
3076 1327 1062 977
AgeI A'CCGG_T
Cuts at: 5273 5273
Size: 6442
AhdI GACnn_n'nnGTC
Cuts at: 3675 5171 3675
Size: 1496 4946
AleI CACnn'nnGTG
Cuts at: 1166 3719 1166
Size: 2553 3889
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6442
AlwNI CAG_nnn'CTG
Cuts at: 2390 4185 5757 2390
Size: 1795 1572 3075
Fragments arranged by size:
3075 1795 1572
ApaLI G'TGCA_C
Cuts at: 4499 5655 4499
Size: 1156 5286
ApoI r'AATT_y
Cuts at: 1493 2440 2848 3708 3751 3977 4129 6238 1493
Size: 947 408 860 43 226 152 2109 1697
Fragments arranged by size:
2109 1697 947 860 408 226 152 43
AseI AT'TA_AT
Cuts at: 160 1032 2601 4996 160
Size: 872 1569 2395 1606
Fragments arranged by size:
2395 1606 1569 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
AvaI C'yCGr_G
Cuts at: 1870 1943 3767 1870
Size: 73 1824 4545
Fragments arranged by size:
4545 1824 73
BamHI G'GATC_C
Cuts at: 3772 4139 3772
Size: 367 6075
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3763 5218 618
Size: 325 217 627 1976 1455 1842
Fragments arranged by size:
1976 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2202 2875 3124 3761 1391
Size: 811 673 249 637 4072
Fragments arranged by size:
4072 811 673 637 249
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3674 928
Size: 971 1775 3696
Fragments arranged by size:
3696 1775 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4633 4667 1903
Size: 34 2696 34 3678
Fragments arranged by size:
3678 2696 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4334 5544 4334
Size: 1210 5232
BclI T'GATC_A
Cuts at: 1548 2581 1548
Size: 1033 5409
BfrBI ATG'CAT
Cuts at: 3407 3659 3407
Size: 252 6190
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5053 136
Size: 107 122 71 631 605 3381 1525
Fragments arranged by size:
3381 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2705 6437 2705
Size: 3732 2710
BlpI GC'TnA_GC
Cuts at: 3859 3859
Size: 6442
Bme1580I G_kGCm'C
Cuts at: 1790 2384 4503 5659 1790
Size: 594 2119 1156 2573
Fragments arranged by size:
2573 2119 1156 594
BmgBI CAC'GTC
Cuts at: 1318 2266 1318
Size: 948 5494
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3558 4173 5131
Size: 416 53 83 399 24 2141 615 958
Cuts at: 5131 442
Size: 1753
Fragments arranged by size:
2141 1753 958 615 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6442
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5102 1239
Size: 3863 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4567 5432 5730 4567
Size: 865 298 5279
Fragments arranged by size:
5279 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5105 882
Size: 617 3606 2219
Fragments arranged by size:
3606 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4233 493
Size: 711 3029 2702
Fragments arranged by size:
3029 2702 711
BsaBI GATnn'nnATC
Cuts at: 6436 6436
Size: 6442
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4249 4631 5266 275
Size: 53 83 186 936 2716 382 635 1451
Fragments arranged by size:
2716 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4875 5273 5547 5694 6105 4875
Size: 398 274 147 411 5212
Fragments arranged by size:
5212 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2709 2739 3461 3491 3629
Size: 30 165 30 1306 30 722 30 138
Cuts at: 3629 3659 1178
Size: 30 3961
Fragments arranged by size:
3961 1306 722 165 138 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6442
BseYI C'CCAG_C
Cuts at: 3108 5645 6141 3108
Size: 2537 496 3409
Fragments arranged by size:
3409 2537 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5869
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3892 4653 5681 1540
Size: 275 186 1891 761 1028 2301
Fragments arranged by size:
2301 1891 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2875 3124 3693 4503 4588 5659 2875
Size: 249 569 810 85 1071 3658
Fragments arranged by size:
3658 1071 810 569 249 85
BsmI GAATG_Cn'
Cuts at: 2599 3953 4046 2599
Size: 1354 93 4995
Fragments arranged by size:
4995 1354 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2202 2384 2875 3124 3693 3761 4503
Size: 399 412 182 491 249 569 68 742
Cuts at: 4503 4588 5659 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 569 491 412 399 249
182 85 68
BspEI T'CCGG_A
Cuts at: 6105 6105
Size: 6442
BspHI T'CATG_A
Cuts at: 4331 6061 4331
Size: 1730 4712
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3797 844
Size: 2953 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4329 1394
Size: 429 2506 3507
Fragments arranged by size:
3507 2506 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3329 4937 5111 65
Size: 1183 2081 1608 174 1396
Fragments arranged by size:
2081 1608 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5086 5273 1700
Size: 110 3276 187 2869
Fragments arranged by size:
3276 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3529 96
Size: 3433 3009
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6442
BssSI C'ACGA_G
Cuts at: 4502 5514 4502
Size: 1012 5430
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2826 794
Size: 2032 4410
BstBI TT'CG_AA
Cuts at: 5246 5246
Size: 6442
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6442
BstYI r'GATC_y
Cuts at: 1972 2614 2705 3772 4139 4524 4541 5993 6079
Size: 642 91 1067 367 385 17 1452 86
Cuts at: 6079 6091 6437 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1067 642 385 367 346 91
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5890
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5703
Fragments arranged by size:
5703 683 56
BtsI GCAGTG_nn'
Cuts at: 3958 4752 4772 3958
Size: 794 20 5628
Fragments arranged by size:
5628 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6442
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6407
DraI TTT'AAA
Cuts at: 2769 3746 2769
Size: 977 5465
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5449 809
Size: 507 4133 1802
Fragments arranged by size:
4133 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2635 3889 4778
Size: 54 1425 50 275 18 805 1254 889
Cuts at: 4778 6407 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1254 889 805 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3889 1537
Size: 275 2077 4090
Fragments arranged by size:
4090 2077 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2058 2190 4372 1353
Size: 705 132 2182 3423
Fragments arranged by size:
3423 2182 705 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5029 5403 5549 387
Size: 1064 138 3440 374 146 1280
Fragments arranged by size:
3440 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2873 3122 2873
Size: 249 6193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4505 5102 5889 6157 1239
Size: 393 2873 597 787 268 1524
Fragments arranged by size:
2873 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3708 3751 4129 3708
Size: 43 378 6021
Fragments arranged by size:
6021 378 43
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2385 2385
Size: 6442
FspI TGC'GCA
Cuts at: 4948 4948
Size: 6442
HaeII r_GCGC'y
Cuts at: 1678 5589 1678
Size: 3911 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2097 2129 2709 2741 3265
Size: 32 135 32 525 32 580 32 524
Cuts at: 3265 3297 3423 3455 3661 3693 5083 5115 5157
Size: 32 126 32 206 32 1390 32 42
Cuts at: 5157 5189 1373
Size: 32 2626
Fragments arranged by size:
2626 1390 580 525 524 206 135 126
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3786 4032 669
Size: 988 281 1848 246 3079
Fragments arranged by size:
3079 1848 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6442
HpaI GTT'AAC
Cuts at: 4032 4032
Size: 6442
KpnI G_GTAC'C
Cuts at: 3767 3767
Size: 6442
MfeI C'AATT_G
Cuts at: 4041 4041
Size: 6442
MluI A'CGCG_T
Cuts at: 6318 6318
Size: 6442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5556 5740 1305
Size: 4251 184 2007
Fragments arranged by size:
4251 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2637 10
Size: 54 1425 343 805 3815
Fragments arranged by size:
3815 1425 805 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3565 3719 4400 4759 4918 518
Size: 648 2399 154 681 359 159 2042
Fragments arranged by size:
2399 2042 681 648 359 159 154
MspA1I CmG'CkG
Cuts at: 1606 3858 4535 5683 5928 1606
Size: 2252 677 1148 245 2120
Fragments arranged by size:
2252 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5890
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6442
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6442
NotI GC'GGCC_GC
Cuts at: 3889 3889
Size: 6442
NsiI A_TGCA'T
Cuts at: 3409 3661 3409
Size: 252 6190
NspI r_CATG'y
Cuts at: 1132 1229 3411 3663 3800 5345 1132
Size: 97 2182 252 137 1545 2229
Fragments arranged by size:
2229 2182 1545 252 137 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6442
PciI A'CATG_T
Cuts at: 5341 5341
Size: 6442
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2633 1108
Size: 1525 4917
PfoI T'CCnGG_A
Cuts at: 2115 2115
Size: 6442
PmeI GTTT'AAAC
Cuts at: 3746 3746
Size: 6442
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4511 4543 6024 6056 4511
Size: 32 1481 32 4897
Fragments arranged by size:
4897 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6442
PsiI TTA'TAA
Cuts at: 2574 4012 2574
Size: 1438 5004
PstI C_TGCA'G
Cuts at: 830 3794 830
Size: 2964 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6442
SacI G_AGCT'C
Cuts at: 2875 3124 2875
Size: 249 6193
SalI G'TCGA_C
Cuts at: 1936 3784 1936
Size: 1848 4594
SapI GCTCTTCn'nnn_
Cuts at: 2190 2190
Size: 6442
SbfI CC_TGCA'GG
Cuts at: 3794 3794
Size: 6442
ScaI AGT'ACT
Cuts at: 4690 6402 4690
Size: 1712 4730
SfcI C'TryA_G
Cuts at: 826 1045 3790 4925 5606 5797 826
Size: 219 2745 1135 681 191 1471
Fragments arranged by size:
2745 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6442
SmaI CCC'GGG
Cuts at: 1872 3769 1872
Size: 1897 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4546 5447 5709 820
Size: 197 926 2603 901 262 1553
Fragments arranged by size:
2603 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4233 493
Size: 3740 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6442
SphI G_CATG'C
Cuts at: 1132 3800 1132
Size: 2668 3774
SspI AAT'ATT
Cuts at: 5 52 2452 4366 5
Size: 47 2400 1914 2081
Fragments arranged by size:
2400 2081 1914 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6442
StyI C'CwwG_G
Cuts at: 513 1065 3881 513
Size: 552 2816 3074
Fragments arranged by size:
3074 2816 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2253 4469 4486 4639 4824 5269 325
Size: 1255 673 2216 17 153 185 445 1498
Fragments arranged by size:
2216 1498 1255 673 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3037 3233 3529
Size: 274 80 33 51 1443 1060 196 296
Cuts at: 3529 4688 6400 96
Size: 1159 1712 138
Fragments arranged by size:
1712 1443 1159 1060 296 274 196 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6402 6434 6402
Size: 32 6410
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6442
XbaI T'CTAG_A
Cuts at: 3778 3778
Size: 6442
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2247 2247
Size: 6442
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6442
XmaI C'CCGG_G
Cuts at: 1870 3767 1870
Size: 1897 4545
XmnI GAAnn'nnTTC
Cuts at: 2837 3167 3419 3587 4571 2837
Size: 330 252 168 984 4708
Fragments arranged by size:
4708 984 330 252 168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4250 276
Size: 53 83 186 936 2716 2468
Fragments arranged by size:
2716 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI