Restriction map for
pFN21AB8381
(Circular) (Six-base) MAPSORT of: pFN21AB8381.seq Check: 7624 from: 1 to: 6211
pFN21AB8381 6211 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4021 278
Size: 53 83 186 936 2485 2468
Fragments arranged by size:
2485 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3554 1937
Size: 1617 4594
Acc65I G'GTAC_C
Cuts at: 3532 3532
Size: 6211
AclI AA'CG_TT
Cuts at: 1563 4338 4711 1563
Size: 2775 373 3063
Fragments arranged by size:
3063 2775 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2039 2375 3527 4274 5658 5926 1632
Size: 407 336 1152 747 1384 268 1917
Fragments arranged by size:
1917 1384 1152 747 407 336 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6014
AflIII A'CryG_T
Cuts at: 1203 5110 6087 1203
Size: 3907 977 1327
Fragments arranged by size:
3907 1327 977
AgeI A'CCGG_T
Cuts at: 5042 5042
Size: 6211
AhdI GACnn_n'nnGTC
Cuts at: 4940 4940
Size: 6211
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6211
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6211
AlwNI CAG_nnn'CTG
Cuts at: 2101 3954 5526 2101
Size: 1853 1572 2786
Fragments arranged by size:
2786 1853 1572
ApaLI G'TGCA_C
Cuts at: 2570 4268 5424 2570
Size: 1698 1156 3357
Fragments arranged by size:
3357 1698 1156
ApoI r'AATT_y
Cuts at: 1493 2292 2448 3399 3520 3746 3898 6007 1493
Size: 799 156 951 121 226 152 2109 1697
Fragments arranged by size:
2109 1697 951 799 226 156 152 121
AseI AT'TA_AT
Cuts at: 160 1032 2337 4765 160
Size: 872 1305 2428 1606
Fragments arranged by size:
2428 1606 1305 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6211
AvaI C'yCGr_G
Cuts at: 1870 1943 2846 3536 1870
Size: 73 903 690 4545
Fragments arranged by size:
4545 903 690 73
AvrII C'CTAG_G
Cuts at: 2492 2492
Size: 6211
BamHI G'GATC_C
Cuts at: 3541 3908 3541
Size: 367 5844
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2111 2623 3532 4987 618
Size: 325 217 627 324 512 909 1455 1842
Fragments arranged by size:
1842 1455 909 627 512 325 324 217
BanII G_rGCy'C
Cuts at: 1391 2017 3530 1391
Size: 626 1513 4072
Fragments arranged by size:
4072 1513 626
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5240
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3005 3039 4402 4436 1903
Size: 34 1068 34 1363 34 3678
Fragments arranged by size:
3678 1363 1068 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4103 5313 4103
Size: 1210 5001
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6211
BfrBI ATG'CAT
Cuts at: 3143 3143
Size: 6211
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4822 136
Size: 107 122 71 631 605 3150 1525
Fragments arranged by size:
3150 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6206 6206
Size: 6211
BlpI GC'TnA_GC
Cuts at: 3003 3339 3628 3003
Size: 336 289 5586
Fragments arranged by size:
5586 336 289
Bme1580I G_kGCm'C
Cuts at: 1790 2574 4272 5428 1790
Size: 784 1698 1156 2573
Fragments arranged by size:
2573 1698 1156 784
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6211
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2111 3942 4900
Size: 416 53 83 399 24 694 1831 958
Cuts at: 4900 442
Size: 1753
Fragments arranged by size:
1831 1753 958 694 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2079 1056
Size: 1023 5188
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2190 2190
Size: 6211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2217 2443 2460 2943 4871 1239
Size: 978 226 17 483 1928 2579
Fragments arranged by size:
2579 1928 978 483 226 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4336 5201 5499 4336
Size: 865 298 5048
Fragments arranged by size:
5048 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4874 882
Size: 617 3375 2219
Fragments arranged by size:
3375 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2856 4002 493
Size: 711 1652 1146 2702
Fragments arranged by size:
2702 1652 1146 711
BsaBI GATnn'nnATC
Cuts at: 3104 3492 6205 3104
Size: 388 2713 3110
Fragments arranged by size:
3110 2713 388
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4018 4400 5035 275
Size: 53 83 186 936 2485 382 635 1451
Fragments arranged by size:
2485 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4644 5042 5316 5463 5874 4644
Size: 398 274 147 411 4981
Fragments arranged by size:
4981 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2516 2546 1178
Size: 30 165 30 1113 30 4843
Fragments arranged by size:
4843 1113 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6211
BseYI C'CCAG_C
Cuts at: 5414 5910 5414
Size: 496 5715
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2081 2751 1163
Size: 573 345 670 4623
Fragments arranged by size:
4623 670 573 345
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3661 4422 5450 1540
Size: 275 186 1660 761 1028 2301
Fragments arranged by size:
2301 1660 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2574 2685 3411 4272 4357 5428 2574
Size: 111 726 861 85 1071 3357
Fragments arranged by size:
3357 1071 861 726 111 85
BsmI GAATG_Cn'
Cuts at: 2304 3722 3815 2304
Size: 1418 93 4700
Fragments arranged by size:
4700 1418 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2017 2574 2685 3411 3530 4272 4357
Size: 399 227 557 111 726 119 742 85
Cuts at: 4357 5428 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 726 557 399 227 119
111 85
BspEI T'CCGG_A
Cuts at: 5874 5874
Size: 6211
BspHI T'CATG_A
Cuts at: 2782 4100 5830 2782
Size: 1318 1730 3163
Fragments arranged by size:
3163 1730 1318
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3566 844
Size: 2722 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4098 1394
Size: 429 2275 3507
Fragments arranged by size:
3507 2275 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2138 4706 4880 65
Size: 1183 890 2568 174 1396
Fragments arranged by size:
2568 1396 1183 890 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4855 5042 1700
Size: 110 3045 187 2869
Fragments arranged by size:
3045 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6211
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6211
BssSI C'ACGA_G
Cuts at: 4271 5283 4271
Size: 1012 5199
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3013 794
Size: 2219 3992
BstBI TT'CG_AA
Cuts at: 2296 5015 2296
Size: 2719 3492
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6211
BstXI CCAn_nnnn'nTGG
Cuts at: 3110 3110
Size: 6211
BstYI r'GATC_y
Cuts at: 1972 3541 3908 4293 4310 5762 5848 5860 6206
Size: 1569 367 385 17 1452 86 12 346
Cuts at: 6206 1972
Size: 1977
Fragments arranged by size:
1977 1569 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2994 2994
Size: 6211
BtgI C'CryG_G
Cuts at: 513 1065 2004 2840 513
Size: 552 939 836 3884
Fragments arranged by size:
3884 939 836 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5472
Fragments arranged by size:
5472 683 56
BtsI GCAGTG_nn'
Cuts at: 2244 3014 3197 3727 4521 4541 2244
Size: 770 183 530 794 20 3914
Fragments arranged by size:
3914 794 770 530 183 20
ClaI AT'CG_AT
Cuts at: 1554 2487 1554
Size: 933 5278
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6176
DraI TTT'AAA
Cuts at: 3515 3515
Size: 6211
DraIII CAC_nnn'GTG
Cuts at: 2832 2832
Size: 6211
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5218 809
Size: 507 3902 1802
Fragments arranged by size:
3902 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2092 2694
Size: 54 1425 50 275 18 201 61 602
Cuts at: 2694 3658 4547 6176 8
Size: 964 889 1629 43
Fragments arranged by size:
1629 1425 964 889 602 275 201 61
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3658 1537
Size: 275 1846 4090
Fragments arranged by size:
4090 1846 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2154 2348 4141 1353
Size: 801 194 1793 3423
Fragments arranged by size:
3423 1793 801 194
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4798 5172 5318 387
Size: 1064 138 3209 374 146 1280
Fragments arranged by size:
3209 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2217 2375 2443 2460 2943 3527
Size: 393 407 178 158 68 17 483 584
Cuts at: 3527 4274 4871 5658 5926 1239
Size: 747 597 787 268 1524
Fragments arranged by size:
1524 787 747 597 584 483 407 393
268 178 158 68 17
EcoO109I rG'GnC_Cy
Cuts at: 2536 2536
Size: 6211
EcoRI G'AATT_C
Cuts at: 2448 3399 3520 3898 2448
Size: 951 121 378 4761
Fragments arranged by size:
4761 951 378 121
EcoRV GAT'ATC
Cuts at: 2464 2464
Size: 6211
FspI TGC'GCA
Cuts at: 4717 4717
Size: 6211
HaeII r_GCGC'y
Cuts at: 1678 5358 1678
Size: 3680 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3148 3180 3245 3277 4852
Size: 32 135 32 1576 32 65 32 1575
Cuts at: 4852 4884 4926 4958 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1576 1575 135 65 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3174 3555 3801 669
Size: 988 281 1236 381 246 3079
Fragments arranged by size:
3079 1236 988 381 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6211
HpaI GTT'AAC
Cuts at: 3174 3801 3174
Size: 627 5584
KpnI G_GTAC'C
Cuts at: 3536 3536
Size: 6211
MfeI C'AATT_G
Cuts at: 3810 3810
Size: 6211
MluI A'CGCG_T
Cuts at: 6087 6087
Size: 6211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2528 5325 5509 1305
Size: 1223 2797 184 2007
Fragments arranged by size:
2797 2007 1223 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4389
Fragments arranged by size:
4389 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3252 4169 4528 4687 518
Size: 648 2086 917 359 159 2042
Fragments arranged by size:
2086 2042 917 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2700 3314 3627 4304 5452 5697 1606
Size: 1094 614 313 677 1148 245 2120
Fragments arranged by size:
2120 1148 1094 677 614 313 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2840 513
Size: 552 939 836 3884
Fragments arranged by size:
3884 939 836 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6211
NheI G'CTAG_C
Cuts at: 1052 2075 1052
Size: 1023 5188
NotI GC'GGCC_GC
Cuts at: 3658 3658
Size: 6211
NsiI A_TGCA'T
Cuts at: 3145 3145
Size: 6211
NspI r_CATG'y
Cuts at: 1132 1229 3453 3569 5114 1132
Size: 97 2224 116 1545 2229
Fragments arranged by size:
2229 2224 1545 116 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6211
PciI A'CATG_T
Cuts at: 5110 5110
Size: 6211
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2101 2879 1108
Size: 902 91 778 4440
Fragments arranged by size:
4440 902 778 91
PfoI T'CCnGG_A
Cuts at: 2084 2084
Size: 6211
PmeI GTTT'AAAC
Cuts at: 3515 3515
Size: 6211
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4280 4312 5793 5825 4280
Size: 32 1481 32 4666
Fragments arranged by size:
4666 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2536 2536
Size: 6211
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6211
PsiI TTA'TAA
Cuts at: 3781 3781
Size: 6211
PstI C_TGCA'G
Cuts at: 830 3121 3204 3563 830
Size: 2291 83 359 3478
Fragments arranged by size:
3478 2291 359 83
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6211
PvuII CAG'CTG
Cuts at: 2700 3314 2700
Size: 614 5597
SalI G'TCGA_C
Cuts at: 1936 3553 1936
Size: 1617 4594
SanDI GG'GwC_CC
Cuts at: 2536 2536
Size: 6211
SbfI CC_TGCA'GG
Cuts at: 3563 3563
Size: 6211
ScaI AGT'ACT
Cuts at: 4459 6171 4459
Size: 1712 4499
SfcI C'TryA_G
Cuts at: 826 1045 2116 3117 3200 3559 4694 5375 5566
Size: 219 1071 1001 83 359 1135 681 191
Cuts at: 5566 826
Size: 1471
Fragments arranged by size:
1471 1135 1071 1001 681 359 219 191
83
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6211
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6211
SmaI CCC'GGG
Cuts at: 1872 3538 1872
Size: 1666 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4315 5216 5478 820
Size: 197 926 2372 901 262 1553
Fragments arranged by size:
2372 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4002 493
Size: 3509 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6211
SphI G_CATG'C
Cuts at: 1132 3569 1132
Size: 2437 3774
SspI AAT'ATT
Cuts at: 5 52 4135 5
Size: 47 4083 2081
Fragments arranged by size:
4083 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6211
StyI C'CwwG_G
Cuts at: 513 1065 2004 2492 2840 3167 3650 513
Size: 552 939 488 348 327 483 3074
Fragments arranged by size:
3074 939 552 488 483 348 327
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2093 4238 4255 4408 4593 5038 325
Size: 1255 513 2145 17 153 185 445 1498
Fragments arranged by size:
2145 1498 1255 513 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2394 2635 4457
Size: 274 80 33 51 1443 417 241 1822
Cuts at: 4457 6169 96
Size: 1712 138
Fragments arranged by size:
1822 1712 1443 417 274 241 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2516 2548 2593 2625 3390 3422 6171 6203 2516
Size: 32 45 32 765 32 2749 32 2524
Fragments arranged by size:
2749 2524 765 45 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6211
XbaI T'CTAG_A
Cuts at: 3298 3547 3298
Size: 249 5962
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6211
XmaI C'CCGG_G
Cuts at: 1870 3536 1870
Size: 1666 4545
XmnI GAAnn'nnTTC
Cuts at: 2926 4340 2926
Size: 1414 4797
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4019 276
Size: 53 83 186 936 2485 2468
Fragments arranged by size:
2485 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI
SfiI SfoI SrfI SwaI XcmI