Restriction map for
pFN21AB8379
(Circular) (Six-base) MAPSORT of: pFN21AB8379.seq Check: 628 from: 1 to: 6547
pFN21AB8379 6547 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2742 3061 2742
Size: 319 6228
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4357 278
Size: 53 83 186 936 2821 2468
Fragments arranged by size:
2821 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2277 3372 3890 1937
Size: 340 1095 518 4594
Fragments arranged by size:
4594 1095 518 340
Acc65I G'GTAC_C
Cuts at: 3868 3868
Size: 6547
AclI AA'CG_TT
Cuts at: 1563 4674 5047 1563
Size: 3111 373 3063
Fragments arranged by size:
3111 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2183 3118 4610 5994 6262 1632
Size: 551 935 1492 1384 268 1917
Fragments arranged by size:
1917 1492 1384 935 551 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6350
AflIII A'CryG_T
Cuts at: 1203 2397 2490 5446 6423 1203
Size: 1194 93 2956 977 1327
Fragments arranged by size:
2956 1327 1194 977 93
AgeI A'CCGG_T
Cuts at: 5378 5378
Size: 6547
AhdI GACnn_n'nnGTC
Cuts at: 3148 5276 3148
Size: 2128 4419
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6547
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2750 2802 1184
Size: 1566 52 4929
Fragments arranged by size:
4929 1566 52
AlwNI CAG_nnn'CTG
Cuts at: 2023 2629 2938 3076 3523 4290 5862 2023
Size: 606 309 138 447 767 1572 2708
Fragments arranged by size:
2708 1572 767 606 447 309 138
ApaI G_GGCC'C
Cuts at: 2180 3127 2180
Size: 947 5600
ApaLI G'TGCA_C
Cuts at: 3791 4604 5760 3791
Size: 813 1156 4578
Fragments arranged by size:
4578 1156 813
ApoI r'AATT_y
Cuts at: 1493 3856 4082 4234 6343 1493
Size: 2363 226 152 2109 1697
Fragments arranged by size:
2363 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5101 160
Size: 872 4069 1606
Fragments arranged by size:
4069 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6547
AvaI C'yCGr_G
Cuts at: 1870 1943 2171 2599 3260 3366 3573 3872 1870
Size: 73 228 428 661 106 207 299 4545
Fragments arranged by size:
4545 661 428 299 228 207 106 73
BamHI G'GATC_C
Cuts at: 3465 3559 3877 4244 3465
Size: 94 318 367 5768
Fragments arranged by size:
5768 367 318 94
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2342 2786 3362 3430 3868
Size: 325 217 627 555 444 576 68 438
Cuts at: 3868 5323 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 576 555 444 438 325
217 68
BanII G_rGCy'C
Cuts at: 1391 2180 2253 3104 3127 3573 3619 3782 3866
Size: 789 73 851 23 446 46 163 84
Cuts at: 3866 1391
Size: 4072
Fragments arranged by size:
4072 851 789 446 163 84 73 46
23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2171 2971 928
Size: 971 272 800 4504
Fragments arranged by size:
4504 971 800 272
BbvCI CC'TCA_GC
Cuts at: 2097 2097
Size: 6547
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4738 4772 1903
Size: 34 2801 34 3678
Fragments arranged by size:
3678 2801 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4439 5649 4439
Size: 1210 5337
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6547
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3338 5158 136
Size: 107 122 71 631 605 1666 1820 1525
Fragments arranged by size:
1820 1666 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6542 6542
Size: 6547
BlpI GC'TnA_GC
Cuts at: 2372 3618 3716 3964 2372
Size: 1246 98 248 4955
Fragments arranged by size:
4955 1246 248 98
Bme1580I G_kGCm'C
Cuts at: 1790 2180 2345 2791 3127 3326 3367 3435 3795
Size: 390 165 446 336 199 41 68 360
Cuts at: 3795 4608 5764 1790
Size: 813 1156 2573
Fragments arranged by size:
2573 1156 813 446 390 360 336 199
165 68 41
BmgBI CAC'GTC
Cuts at: 1318 2491 1318
Size: 1173 5374
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3544 4278 5236
Size: 416 53 83 399 24 2127 734 958
Cuts at: 5236 442
Size: 1753
Fragments arranged by size:
2127 1753 958 734 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6547
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3035 3035
Size: 6547
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2856 3671 3796 5207 1239
Size: 1617 815 125 1411 2579
Fragments arranged by size:
2579 1617 1411 815 125
Bpu10I CC'TnA_GC
Cuts at: 2097 3290 2097
Size: 1193 5354
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2952 4672 5537 5835 2952
Size: 1720 865 298 3664
Fragments arranged by size:
3664 1720 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2618 3570 5210 882
Size: 617 1119 952 1640 2219
Fragments arranged by size:
2219 1640 1119 952 617
BsaAI yAC'GTr
Cuts at: 493 1204 2959 4338 493
Size: 711 1755 1379 2702
Fragments arranged by size:
2702 1755 1379 711
BsaBI GATnn'nnATC
Cuts at: 6541 6541
Size: 6547
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4354 4736 5371 275
Size: 53 83 186 936 2821 382 635 1451
Fragments arranged by size:
2821 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2245 2982 4980 5378 5652 5799 6210 2245
Size: 737 1998 398 274 147 411 2582
Fragments arranged by size:
2582 1998 737 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2043 2073 3737 3767 1178
Size: 30 165 30 640 30 1664 30 3958
Fragments arranged by size:
3958 1664 640 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2054 2057 2100 2470 3515 1186
Size: 865 3 3 43 370 1045 4218
Fragments arranged by size:
4218 1045 865 370 43 3 3
BseYI C'CCAG_C
Cuts at: 2747 3333 3341 3516 5750 6246 2747
Size: 586 8 175 2234 496 3048
Fragments arranged by size:
3048 2234 586 496 175 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2012 2666 1163
Size: 573 276 654 5044
Fragments arranged by size:
5044 654 573 276
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3647 3997 4758 5786 1540
Size: 275 186 1646 350 761 1028 2301
Fragments arranged by size:
2301 1646 1028 761 350 275 186
BsiHKAI G_wGCw'C
Cuts at: 3782 3795 4608 4693 5764 3782
Size: 13 813 85 1071 4565
Fragments arranged by size:
4565 1071 813 85 13
BsmI GAATG_Cn'
Cuts at: 2154 4058 4151 2154
Size: 1904 93 4550
Fragments arranged by size:
4550 1904 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2180 2253 2345 2791 3104 3127 3326
Size: 399 390 73 92 446 313 23 199
Cuts at: 3326 3367 3435 3573 3619 3782 3795 3866 4608
Size: 41 68 138 46 163 13 71 742
Cuts at: 4608 4693 5764 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 446 399 390 313 199
163 138 92 85 73 71 68 46
41 23 13
BspEI T'CCGG_A
Cuts at: 6210 6210
Size: 6547
BspHI T'CATG_A
Cuts at: 4436 6166 4436
Size: 1730 4817
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2742 2923 3061 3648 3902 844
Size: 1898 181 138 587 254 3489
Fragments arranged by size:
3489 1898 587 254 181 138
BsrBI CCG'CTC
Cuts at: 1394 1823 2784 4434 1394
Size: 429 961 1650 3507
Fragments arranged by size:
3507 1650 961 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2611 5042 5216 65
Size: 1183 1363 2431 174 1396
Fragments arranged by size:
2431 1396 1363 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2142 5191 5378 1700
Size: 110 332 3049 187 2869
Fragments arranged by size:
3049 2869 332 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6547
BssHII G'CGCG_C
Cuts at: 1925 3670 1925
Size: 1745 4802
BssSI C'ACGA_G
Cuts at: 4607 5619 4607
Size: 1012 5535
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2023 2644 2962 794
Size: 1229 621 318 4379
Fragments arranged by size:
4379 1229 621 318
BstBI TT'CG_AA
Cuts at: 5351 5351
Size: 6547
BstEII G'GTnAC_C
Cuts at: 1184 2672 1184
Size: 1488 5059
BstXI CCAn_nnnn'nTGG
Cuts at: 2848 3341 2848
Size: 493 6054
BstYI r'GATC_y
Cuts at: 1972 2448 3465 3559 3877 4244 4629 4646 6098
Size: 476 1017 94 318 367 385 17 1452
Cuts at: 6098 6184 6196 6542 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1017 476 385 367 346 318
94 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3278 3278
Size: 6547
BtgI C'CryG_G
Cuts at: 513 1065 2220 2808 3061 3327 3436 513
Size: 552 1155 588 253 266 109 3624
Fragments arranged by size:
3624 1155 588 552 266 253 109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3309 487
Size: 683 56 2083 3725
Fragments arranged by size:
3725 2083 683 56
BtsI GCAGTG_nn'
Cuts at: 2024 2645 4063 4857 4877 2024
Size: 621 1418 794 20 3694
Fragments arranged by size:
3694 1418 794 621 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6547
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6512
DraI TTT'AAA
Cuts at: 3851 3851
Size: 6547
DraIII CAC_nnn'GTG
Cuts at: 2860 2860
Size: 6547
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3314 5554 809
Size: 507 1998 2240 1802
Fragments arranged by size:
2240 1998 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2500 3177 3644
Size: 54 1425 50 275 18 670 677 467
Cuts at: 3644 3994 4883 6512 8
Size: 350 889 1629 43
Fragments arranged by size:
1629 1425 889 677 670 467 350 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3644 3994 1537
Size: 275 1832 350 4090
Fragments arranged by size:
4090 1832 350 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4477 1353
Size: 3124 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3758 5134 5508 5654 387
Size: 1064 138 2169 1376 374 146 1280
Fragments arranged by size:
2169 1376 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3780 3780
Size: 6547
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2856 3118 3671 3796 4610 5207
Size: 393 551 673 262 553 125 814 597
Cuts at: 5207 5994 6262 1239
Size: 787 268 1524
Fragments arranged by size:
1524 814 787 673 597 553 551 393
268 262 125
EcoNI CCTnn'n_nnAGG
Cuts at: 2265 3281 2265
Size: 1016 5531
EcoO109I rG'GnC_Cy
Cuts at: 2010 2176 2177 2225 2475 2596 2755 2935 3037
Size: 166 1 48 250 121 159 180 102
Cuts at: 3037 3123 3124 3265 3286 3586 2010
Size: 86 1 141 21 300 4971
Fragments arranged by size:
4971 300 250 180 166 159 141 121
102 86 48 21 1 1
EcoRI G'AATT_C
Cuts at: 3856 4234 3856
Size: 378 6169
FspI TGC'GCA
Cuts at: 5053 5053
Size: 6547
HaeII r_GCGC'y
Cuts at: 1678 3723 3751 5694 1678
Size: 2045 28 1943 2531
Fragments arranged by size:
2531 2045 1943 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2666 2698 3086 3118 3134
Size: 32 135 32 1094 32 388 32 16
Cuts at: 3134 3166 3821 3853 5188 5220 5262 5294 1373
Size: 32 655 32 1335 32 42 32 2626
Fragments arranged by size:
2626 1335 1094 655 388 135 42 32
32 32 32 32 32 32 32 16
HincII GTy'rAC
Cuts at: 669 1657 1938 3891 4137 669
Size: 988 281 1953 246 3079
Fragments arranged by size:
3079 1953 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6547
HpaI GTT'AAC
Cuts at: 4137 4137
Size: 6547
KpnI G_GTAC'C
Cuts at: 3872 3872
Size: 6547
MfeI C'AATT_G
Cuts at: 4146 4146
Size: 6547
MluI A'CGCG_T
Cuts at: 6423 6423
Size: 6547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5661 5845 1305
Size: 4356 184 2007
Fragments arranged by size:
4356 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4725
Fragments arranged by size:
4725 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2348 4505 4864 5023 518
Size: 648 1182 2157 359 159 2042
Fragments arranged by size:
2157 2042 1182 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2848 3337 3649 3774 3963 4640 5788 6033
Size: 1242 489 312 125 189 677 1148 245
Cuts at: 6033 1606
Size: 2120
Fragments arranged by size:
2120 1242 1148 677 489 312 245 189
125
NaeI GCC'GGC
Cuts at: 2144 2144
Size: 6547
NcoI C'CATG_G
Cuts at: 513 1065 2220 513
Size: 552 1155 4840
Fragments arranged by size:
4840 1155 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6547
NgoMIV G'CCGG_C
Cuts at: 2142 2142
Size: 6547
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6547
NotI GC'GGCC_GC
Cuts at: 3644 3994 3644
Size: 350 6197
NspI r_CATG'y
Cuts at: 1132 1229 2731 3905 5450 1132
Size: 97 1502 1174 1545 2229
Fragments arranged by size:
2229 1545 1502 1174 97
PasI CC'CwG_GG
Cuts at: 1348 2802 3358 1348
Size: 1454 556 4537
Fragments arranged by size:
4537 1454 556
PciI A'CATG_T
Cuts at: 5446 5446
Size: 6547
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2314 2860 1108
Size: 1206 546 4795
Fragments arranged by size:
4795 1206 546
PfoI T'CCnGG_A
Cuts at: 2616 2616
Size: 6547
PmeI GTTT'AAAC
Cuts at: 3851 3851
Size: 6547
PmlI CAC'GTG
Cuts at: 2959 2959
Size: 6547
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3437 3469 4616 4648 6129 6161 3437
Size: 32 1147 32 1481 32 3823
Fragments arranged by size:
3823 1481 1147 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2225 2935 3265 2225
Size: 710 330 5507
Fragments arranged by size:
5507 710 330
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6547
PsiI TTA'TAA
Cuts at: 4117 4117
Size: 6547
PspOMI G'GGCC_C
Cuts at: 2176 3123 2176
Size: 947 5600
PstI C_TGCA'G
Cuts at: 830 2640 2781 3072 3899 830
Size: 1810 141 291 827 3478
Fragments arranged by size:
3478 1810 827 291 141
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6547
PvuII CAG'CTG
Cuts at: 2848 3337 2848
Size: 489 6058
SacI G_AGCT'C
Cuts at: 3782 3782
Size: 6547
SalI G'TCGA_C
Cuts at: 1936 3889 1936
Size: 1953 4594
SanDI GG'GwC_CC
Cuts at: 2225 2225
Size: 6547
SbfI CC_TGCA'GG
Cuts at: 3072 3899 3072
Size: 827 5720
ScaI AGT'ACT
Cuts at: 4795 6507 4795
Size: 1712 4835
SexAI A'CCwGG_T
Cuts at: 3843 3843
Size: 6547
SfcI C'TryA_G
Cuts at: 826 1045 2467 2636 2777 3068 3817 3895 5030
Size: 219 1422 169 141 291 749 78 1135
Cuts at: 5030 5711 5902 826
Size: 681 191 1471
Fragments arranged by size:
1471 1422 1135 749 681 291 219 191
169 141 78
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6547
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6547
SmaI CCC'GGG
Cuts at: 1872 2173 2601 3368 3874 1872
Size: 301 428 767 506 4545
Fragments arranged by size:
4545 767 506 428 301
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2967 4651 5552 5814 820
Size: 197 926 1024 1684 901 262 1553
Fragments arranged by size:
1684 1553 1024 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4338 493
Size: 3845 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6547
SphI G_CATG'C
Cuts at: 1132 2731 3905 1132
Size: 1599 1174 3774
Fragments arranged by size:
3774 1599 1174
SspI AAT'ATT
Cuts at: 5 52 4471 5
Size: 47 4419 2081
Fragments arranged by size:
4419 2081 47
StuI AGG'CCT
Cuts at: 1517 3680 1517
Size: 2163 4384
StyI C'CwwG_G
Cuts at: 513 1065 2220 2261 2484 3582 3986 513
Size: 552 1155 41 223 1098 404 3074
Fragments arranged by size:
3074 1155 1098 552 404 223 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2069 2233 2632 3071 4574 4591 4744
Size: 1255 489 164 399 439 1503 17 153
Cuts at: 4744 4929 5374 325
Size: 185 445 1498
Fragments arranged by size:
1503 1498 1255 489 445 439 399 185
164 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4793 6505 96
Size: 274 80 33 51 1443 2816 1712 138
Fragments arranged by size:
2816 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2021 2043 2053 2075 2723 2755 3383 3415 3544
Size: 22 10 22 648 32 628 32 129
Cuts at: 3544 3576 3737 3769 6507 6539 2021
Size: 32 161 32 2738 32 2029
Fragments arranged by size:
2738 2029 648 628 161 129 32 32
32 32 32 22 22 10
Tth111I GACn'n_nGTC
Cuts at: 1316 2229 1316
Size: 913 5634
XbaI T'CTAG_A
Cuts at: 3883 3883
Size: 6547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2815 2815
Size: 6547
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6547
XmaI C'CCGG_G
Cuts at: 1870 2171 2599 3366 3872 1870
Size: 301 428 767 506 4545
Fragments arranged by size:
4545 767 506 428 301
XmnI GAAnn'nnTTC
Cuts at: 3509 3698 3806 4676 3509
Size: 189 108 870 5380
Fragments arranged by size:
5380 870 189 108
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4355 276
Size: 53 83 186 936 2821 2468
Fragments arranged by size:
2821 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PspXI PsrI RsrII SacII SapI
SfiI SfoI SrfI SwaI