Restriction map for
pFN21AB8378
(Circular) (Six-base) MAPSORT of: pFN21AB8378.seq Check: 3583 from: 1 to: 6379
pFN21AB8378 6379 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2109 2167 3583 2109
Size: 58 1416 4905
Fragments arranged by size:
4905 1416 58
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4189 278
Size: 53 83 186 936 2653 2468
Fragments arranged by size:
2653 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3722 1937
Size: 1785 4594
Acc65I G'GTAC_C
Cuts at: 3700 3700
Size: 6379
AclI AA'CG_TT
Cuts at: 1563 4506 4879 1563
Size: 2943 373 3063
Fragments arranged by size:
3063 2943 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2293 2503 3041 4442 5826 6094 1632
Size: 661 210 538 1401 1384 268 1917
Fragments arranged by size:
1917 1401 1384 661 538 268 210
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6182
AflIII A'CryG_T
Cuts at: 1203 3268 5278 6255 1203
Size: 2065 2010 977 1327
Fragments arranged by size:
2065 2010 1327 977
AgeI A'CCGG_T
Cuts at: 3676 5210 3676
Size: 1534 4845
AhdI GACnn_n'nnGTC
Cuts at: 5108 5108
Size: 6379
AleI CACnn'nnGTG
Cuts at: 1166 3239 1166
Size: 2073 4306
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6379
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2963 2995 2963
Size: 32 6347
AlwNI CAG_nnn'CTG
Cuts at: 2269 3451 4122 5694 2269
Size: 1182 671 1572 2954
Fragments arranged by size:
2954 1572 1182 671
ApaI G_GGCC'C
Cuts at: 2301 2301
Size: 6379
ApaLI G'TGCA_C
Cuts at: 2347 4436 5592 2347
Size: 2089 1156 3134
Fragments arranged by size:
3134 2089 1156
ApoI r'AATT_y
Cuts at: 1493 2735 3688 3914 4066 6175 1493
Size: 1242 953 226 152 2109 1697
Fragments arranged by size:
2109 1697 1242 953 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4933 160
Size: 872 3901 1606
Fragments arranged by size:
3901 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
AvaI C'yCGr_G
Cuts at: 1870 1943 2042 2455 2709 3628 3704 1870
Size: 73 99 413 254 919 76 4545
Fragments arranged by size:
4545 919 413 254 99 76 73
BamHI G'GATC_C
Cuts at: 3102 3709 4076 3102
Size: 607 367 5405
Fragments arranged by size:
5405 607 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2097 3244 3700 5155 618
Size: 325 217 627 310 1147 456 1455 1842
Fragments arranged by size:
1842 1455 1147 627 456 325 310 217
BanII G_rGCy'C
Cuts at: 1391 2018 2301 2481 2547 3100 3495 3537 3698
Size: 627 283 180 66 553 395 42 161
Cuts at: 3698 1391
Size: 4072
Fragments arranged by size:
4072 627 553 395 283 180 161 66
42
BbeI G_GCGC'C
Cuts at: 3248 3248
Size: 6379
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2224 928
Size: 971 325 5083
Fragments arranged by size:
5083 971 325
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4570 4604 1903
Size: 34 2633 34 3678
Fragments arranged by size:
3678 2633 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4271 5481 4271
Size: 1210 5169
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6379
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3618 3656 4990
Size: 107 122 71 631 605 1946 38 1334
Cuts at: 4990 136
Size: 1525
Fragments arranged by size:
1946 1525 1334 631 605 122 107 71
38
BglII A'GATC_T
Cuts at: 3045 6374 3045
Size: 3329 3050
BlpI GC'TnA_GC
Cuts at: 2087 3796 2087
Size: 1709 4670
Bme1580I G_kGCm'C
Cuts at: 1790 2301 2351 3525 4440 5596 1790
Size: 511 50 1174 915 1156 2573
Fragments arranged by size:
2573 1174 1156 915 511 50
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6379
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2782 4110 5068
Size: 416 53 83 399 24 1365 1328 958
Cuts at: 5068 442
Size: 1753
Fragments arranged by size:
1753 1365 1328 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6379
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2470 2470
Size: 6379
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2561 5039 1239
Size: 1322 2478 2579
Fragments arranged by size:
2579 2478 1322
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2384 2852 3521 4504 5369 5667 2384
Size: 468 669 983 865 298 3096
Fragments arranged by size:
3096 983 865 669 468 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2955 5042 882
Size: 617 1456 2087 2219
Fragments arranged by size:
2219 2087 1456 617
BsaAI yAC'GTr
Cuts at: 493 1204 2473 4170 493
Size: 711 1269 1697 2702
Fragments arranged by size:
2702 1697 1269 711
BsaBI GATnn'nnATC
Cuts at: 6373 6373
Size: 6379
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3245 3468 4186 4568
Size: 53 83 186 936 1712 223 718 382
Cuts at: 4568 5203 275
Size: 635 1451
Fragments arranged by size:
1712 1451 936 718 635 382 223 186
83 53
BsaWI w'CCGG_w
Cuts at: 2199 3676 4812 5210 5484 5631 6042 2199
Size: 1477 1136 398 274 147 411 2536
Fragments arranged by size:
2536 1477 1136 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2838 2868 2963 2993 1178
Size: 30 165 30 1435 30 95 30 4564
Fragments arranged by size:
4564 1435 165 95 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2270 2681 2768 2789 3299 1186
Size: 1084 411 87 21 510 4266
Fragments arranged by size:
4266 1084 510 411 87 21
BseYI C'CCAG_C
Cuts at: 2135 2300 5582 6078 2135
Size: 165 3282 496 2436
Fragments arranged by size:
3282 2436 496 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2195 2282 2969 3655 1163
Size: 573 459 87 687 686 3887
Fragments arranged by size:
3887 687 686 573 459 87
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3282 3829 4590 5618 1540
Size: 275 186 1281 547 761 1028 2301
Fragments arranged by size:
2301 1281 1028 761 547 275 186
BsiHKAI G_wGCw'C
Cuts at: 2351 2481 2547 3293 3537 4440 4525 5596 2351
Size: 130 66 746 244 903 85 1071 3134
Fragments arranged by size:
3134 1071 903 746 244 130 85 66
BsmI GAATG_Cn'
Cuts at: 3890 3983 3890
Size: 93 6286
BsmBI CGTCTCn'nnnn_
Cuts at: 2818 2818
Size: 6379
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2301 2351 2481 2547 3100 3293
Size: 399 228 283 50 130 66 553 193
Cuts at: 3293 3495 3525 3537 3698 4440 4525 5596 1391
Size: 202 30 12 161 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 553 399 283 228 202
193 161 130 85 66 50 30 12
BspEI T'CCGG_A
Cuts at: 2199 6042 2199
Size: 3843 2536
BspHI T'CATG_A
Cuts at: 4268 5998 4268
Size: 1730 4649
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2109 2167 3436 3583 3734 844
Size: 1265 58 1269 147 151 3489
Fragments arranged by size:
3489 1269 1265 151 147 58
BsrBI CCG'CTC
Cuts at: 1394 1823 2119 4266 1394
Size: 429 296 2147 3507
Fragments arranged by size:
3507 2147 429 296
BsrDI GCAATG_nn'
Cuts at: 65 1248 4874 5048 65
Size: 1183 3626 174 1396
Fragments arranged by size:
3626 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2094 3676 5023 5210 1700
Size: 110 284 1582 1347 187 2869
Fragments arranged by size:
2869 1582 1347 284 187 110
BsrGI T'GTAC_A
Cuts at: 96 2583 96
Size: 2487 3892
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6379
BssSI C'ACGA_G
Cuts at: 4439 5451 4439
Size: 1012 5367
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2269 2346 3551 794
Size: 1475 77 1205 3622
Fragments arranged by size:
3622 1475 1205 77
BstBI TT'CG_AA
Cuts at: 5183 5183
Size: 6379
BstEII G'GTnAC_C
Cuts at: 1184 2380 3343 1184
Size: 1196 963 4220
Fragments arranged by size:
4220 1196 963
BstXI CCAn_nnnn'nTGG
Cuts at: 2500 3241 2500
Size: 741 5638
BstYI r'GATC_y
Cuts at: 1972 2919 3045 3102 3709 4076 4461 4478 5930
Size: 947 126 57 607 367 385 17 1452
Cuts at: 5930 6016 6028 6374 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 947 607 385 367 346 126
86 57 17 12
Bsu36I CC'TnA_GG
Cuts at: 3153 3153
Size: 6379
BtgI C'CryG_G
Cuts at: 513 1065 2931 2966 3036 3482 513
Size: 552 1866 35 70 446 3410
Fragments arranged by size:
3410 1866 552 446 70 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5640
Fragments arranged by size:
5640 683 56
BtsI GCAGTG_nn'
Cuts at: 3895 4689 4709 3895
Size: 794 20 5565
Fragments arranged by size:
5565 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6379
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6344
DraI TTT'AAA
Cuts at: 3683 3683
Size: 6379
DraIII CAC_nnn'GTG
Cuts at: 2881 3488 2881
Size: 607 5772
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3446 3467 5386 809
Size: 507 2130 21 1919 1802
Fragments arranged by size:
2130 1919 1802 507 21
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2934 3279 3354
Size: 54 1425 50 275 18 1104 345 75
Cuts at: 3354 3561 3576 3826 4715 6344 8
Size: 207 15 250 889 1629 43
Fragments arranged by size:
1629 1425 1104 889 345 275 250 207
75 54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 3279 3826 1537
Size: 275 1467 547 4090
Fragments arranged by size:
4090 1467 547 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2522 2816 4309 1353
Size: 1169 294 1493 3423
Fragments arranged by size:
3423 1493 1169 294
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4966 5340 5486 387
Size: 1064 138 3377 374 146 1280
Fragments arranged by size:
3377 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2479 2545 3535 2479
Size: 66 990 5323
Fragments arranged by size:
5323 990 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2293 2503 2561 3041 4442 5039 5826
Size: 393 661 210 58 480 1401 597 787
Cuts at: 5826 6094 1239
Size: 268 1524
Fragments arranged by size:
1524 1401 787 661 597 480 393 268
210 58
EcoO109I rG'GnC_Cy
Cuts at: 2512 3448 3583 2512
Size: 936 135 5308
Fragments arranged by size:
5308 936 135
EcoRI G'AATT_C
Cuts at: 2735 3688 4066 2735
Size: 953 378 5048
Fragments arranged by size:
5048 953 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2533 2740 2533
Size: 207 6172
FspI TGC'GCA
Cuts at: 4885 4885
Size: 6379
HaeII r_GCGC'y
Cuts at: 1678 3248 3560 5526 1678
Size: 1570 312 1966 2531
Fragments arranged by size:
2531 1966 1570 312
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2662 2690 2694 2722 2838
Size: 32 135 32 1090 28 4 28 116
Cuts at: 2838 2870 3087 3119 5020 5052 5094 5126 1373
Size: 32 217 32 1901 32 42 32 2626
Fragments arranged by size:
2626 1901 1090 217 135 116 42 32
32 32 32 32 32 28 28 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2557 3723 3969 669
Size: 988 281 619 1166 246 3079
Fragments arranged by size:
3079 1166 988 619 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6379
HpaI GTT'AAC
Cuts at: 3969 3969
Size: 6379
KasI G'GCGC_C
Cuts at: 3244 3244
Size: 6379
KpnI G_GTAC'C
Cuts at: 3704 3704
Size: 6379
MfeI C'AATT_G
Cuts at: 3978 3978
Size: 6379
MluI A'CGCG_T
Cuts at: 3268 6255 3268
Size: 2987 3392
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2154 2553 5493 5677 1305
Size: 849 399 2940 184 2007
Fragments arranged by size:
2940 2007 849 399 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2936 3563 10
Size: 54 1425 343 1104 627 2826
Fragments arranged by size:
2826 1425 1104 627 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2435 3239 3410 3440 4337 4696 4855
Size: 648 1269 804 171 30 897 359 159
Cuts at: 4855 518
Size: 2042
Fragments arranged by size:
2042 1269 897 804 648 359 171 159
30
MspA1I CmG'CkG
Cuts at: 1606 2091 2304 2313 2392 2578 2661 2968 2976
Size: 485 213 9 79 186 83 307 8
Cuts at: 2976 3795 4472 5620 5865 1606
Size: 819 677 1148 245 2120
Fragments arranged by size:
2120 1148 819 677 485 307 245 213
186 83 79 9 8
NaeI GCC'GGC
Cuts at: 2096 2096
Size: 6379
NarI GG'CG_CC
Cuts at: 3245 3245
Size: 6379
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5827
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6379
NgoMIV G'CCGG_C
Cuts at: 2094 2094
Size: 6379
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6379
NotI GC'GGCC_GC
Cuts at: 3826 3826
Size: 6379
NspI r_CATG'y
Cuts at: 1132 1229 3445 3737 5282 1132
Size: 97 2216 292 1545 2229
Fragments arranged by size:
2229 2216 1545 292 97
PasI CC'CwG_GG
Cuts at: 1348 2353 3213 1348
Size: 1005 860 4514
Fragments arranged by size:
4514 1005 860
PciI A'CATG_T
Cuts at: 5278 5278
Size: 6379
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3184 1108
Size: 2076 4303
PfoI T'CCnGG_A
Cuts at: 2538 2892 3450 3501 3528 2538
Size: 354 558 51 27 5389
Fragments arranged by size:
5389 558 354 51 27
PmeI GTTT'AAAC
Cuts at: 3683 3683
Size: 6379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2963 2995 4448 4480 5961 5993 2963
Size: 32 1453 32 1481 32 3349
Fragments arranged by size:
3349 1481 1453 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2512 3448 3583 2512
Size: 936 135 5308
Fragments arranged by size:
5308 936 135
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6379
PsiI TTA'TAA
Cuts at: 2835 3949 2835
Size: 1114 5265
PspOMI G'GGCC_C
Cuts at: 2297 2297
Size: 6379
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2453 2485 2795 2827 2453
Size: 32 310 32 6005
Fragments arranged by size:
6005 310 32 32
PstI C_TGCA'G
Cuts at: 830 2154 2660 3731 830
Size: 1324 506 1071 3478
Fragments arranged by size:
3478 1324 1071 506
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6379
PvuII CAG'CTG
Cuts at: 2091 2313 2578 2661 2976 2091
Size: 222 265 83 315 5494
Fragments arranged by size:
5494 315 265 222 83
SacI G_AGCT'C
Cuts at: 2481 2547 3537 2481
Size: 66 990 5323
Fragments arranged by size:
5323 990 66
SacII CC_GC'GG
Cuts at: 2969 2969
Size: 6379
SalI G'TCGA_C
Cuts at: 1936 3721 1936
Size: 1785 4594
SbfI CC_TGCA'GG
Cuts at: 3731 3731
Size: 6379
ScaI AGT'ACT
Cuts at: 3605 4627 6339 3605
Size: 1022 1712 3645
Fragments arranged by size:
3645 1712 1022
SfcI C'TryA_G
Cuts at: 826 1045 2150 2548 2656 3607 3727 4862 5543
Size: 219 1105 398 108 951 120 1135 681
Cuts at: 5543 5734 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1105 951 681 398 219 191
120 108
SfoI GGC'GCC
Cuts at: 3246 3246
Size: 6379
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6379
SmaI CCC'GGG
Cuts at: 1872 2044 3706 1872
Size: 172 1662 4545
Fragments arranged by size:
4545 1662 172
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2399 2709 2867 3536 4483 5384
Size: 197 926 456 310 158 669 947 901
Cuts at: 5384 5646 820
Size: 262 1553
Fragments arranged by size:
1553 947 926 901 669 456 310 262
197 158
SnaBI TAC'GTA
Cuts at: 493 4170 493
Size: 3677 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6379
SphI G_CATG'C
Cuts at: 1132 3737 1132
Size: 2605 3774
SspI AAT'ATT
Cuts at: 5 52 4303 5
Size: 47 4251 2081
Fragments arranged by size:
4251 2081 47
StuI AGG'CCT
Cuts at: 1517 2630 2677 2768 3194 3621 1517
Size: 1113 47 91 426 427 4275
Fragments arranged by size:
4275 1113 427 426 91 47
StyI C'CwwG_G
Cuts at: 513 1065 2226 3258 3818 513
Size: 552 1161 1032 560 3074
Fragments arranged by size:
3074 1161 1032 560 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2161 3362 3629 4406 4423 4576 4761
Size: 1255 581 1201 267 777 17 153 185
Cuts at: 4761 5206 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1201 777 581 445 267 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2583 2883 3603
Size: 274 80 33 51 1443 606 300 720
Cuts at: 3603 4625 6337 96
Size: 1022 1712 138
Fragments arranged by size:
1712 1443 1022 720 606 300 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3149 3181 6339 6371 3149
Size: 32 3158 32 3157
Fragments arranged by size:
3158 3157 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3467 1316
Size: 2151 4228
XbaI T'CTAG_A
Cuts at: 3715 3715
Size: 6379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3409 3571 3409
Size: 162 6217
XhoI C'TCGA_G
Cuts at: 1943 2709 1943
Size: 766 5613
XmaI C'CCGG_G
Cuts at: 1870 2042 3704 1870
Size: 172 1662 4545
Fragments arranged by size:
4545 1662 172
XmnI GAAnn'nnTTC
Cuts at: 4508 4508
Size: 6379
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4187 276
Size: 53 83 186 936 2653 2468
Fragments arranged by size:
2653 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BstZ17I EcoNI EcoRV FseI FspAI NruI NsiI PacI
PmlI PspXI RsrII SanDI SapI SexAI SfiI SrfI
SwaI