Restriction map for
pFN21AB8376
(Circular) (Six-base) MAPSORT of: pFN21AB8376.seq Check: 7980 from: 1 to: 6583
pFN21AB8376 6583 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2587 2587
Size: 6583
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2712 4393 278
Size: 53 83 186 936 1176 1681 2468
Fragments arranged by size:
2468 1681 1176 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3926 1937
Size: 1989 4594
Acc65I G'GTAC_C
Cuts at: 3904 3904
Size: 6583
AclI AA'CG_TT
Cuts at: 1563 4710 5083 1563
Size: 3147 373 3063
Fragments arranged by size:
3147 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4646 6030 6298 1632
Size: 3014 1384 268 1917
Fragments arranged by size:
3014 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6386
AflIII A'CryG_T
Cuts at: 1203 5482 6459 1203
Size: 4279 977 1327
Fragments arranged by size:
4279 1327 977
AgeI A'CCGG_T
Cuts at: 5414 5414
Size: 6583
AhdI GACnn_n'nnGTC
Cuts at: 5312 5312
Size: 6583
AleI CACnn'nnGTG
Cuts at: 1166 2070 2465 2591 1166
Size: 904 395 126 5158
Fragments arranged by size:
5158 904 395 126
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2327 2609 1184
Size: 1143 282 5158
Fragments arranged by size:
5158 1143 282
AlwNI CAG_nnn'CTG
Cuts at: 3100 3295 4326 5898 3100
Size: 195 1031 1572 3785
Fragments arranged by size:
3785 1572 1031 195
ApaLI G'TGCA_C
Cuts at: 4640 5796 4640
Size: 1156 5427
ApoI r'AATT_y
Cuts at: 1493 3892 4118 4270 6379 1493
Size: 2399 226 152 2109 1697
Fragments arranged by size:
2399 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5137 160
Size: 872 4105 1606
Fragments arranged by size:
4105 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6583
AvaI C'yCGr_G
Cuts at: 1870 1943 2055 2330 3155 3276 3908 1870
Size: 73 112 275 825 121 632 4545
Fragments arranged by size:
4545 825 632 275 121 112 73
BamHI G'GATC_C
Cuts at: 3913 4280 3913
Size: 367 6216
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2072 2437 2485 2585 3488
Size: 325 217 627 285 365 48 100 903
Cuts at: 3488 3557 3703 3729 3904 5359 618
Size: 69 146 26 175 1455 1842
Fragments arranged by size:
1842 1455 903 627 365 325 285 217
175 146 100 69 48 26
BanII G_rGCy'C
Cuts at: 1391 2553 2768 2964 3523 3641 3774 3902 1391
Size: 1162 215 196 559 118 133 128 4072
Fragments arranged by size:
4072 1162 559 215 196 133 128 118
BbeI G_GCGC'C
Cuts at: 3492 3561 3733 3492
Size: 69 172 6342
Fragments arranged by size:
6342 172 69
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2215 2907 928
Size: 971 316 692 4604
Fragments arranged by size:
4604 971 692 316
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3388 3422 4774 4808 1903
Size: 34 1451 34 1352 34 3678
Fragments arranged by size:
3678 1451 1352 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4475 5685 4475
Size: 1210 5373
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6583
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2921 5194 136
Size: 107 122 71 631 605 1249 2273 1525
Fragments arranged by size:
2273 1525 1249 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6578 6578
Size: 6583
BlpI GC'TnA_GC
Cuts at: 2315 2519 4000 2315
Size: 204 1481 4898
Fragments arranged by size:
4898 1481 204
Bme1580I G_kGCm'C
Cuts at: 1790 2442 2588 2622 3706 4644 5800 1790
Size: 652 146 34 1084 938 1156 2573
Fragments arranged by size:
2573 1156 1084 938 652 146 34
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6583
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2077 4314 5272
Size: 416 53 83 399 24 660 2237 958
Cuts at: 5272 442
Size: 1753
Fragments arranged by size:
2237 1753 958 660 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3857 1056
Size: 2801 3782
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2758 3049 3813 2758
Size: 291 764 5528
Fragments arranged by size:
5528 764 291
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2894 2900 3155 3275 3521 5243 1239
Size: 1655 6 255 120 246 1722 2579
Fragments arranged by size:
2579 1722 1655 255 246 120 6
Bpu10I CC'TnA_GC
Cuts at: 2514 3788 2514
Size: 1274 5309
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4708 5573 5871 4708
Size: 865 298 5420
Fragments arranged by size:
5420 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3037 5246 882
Size: 617 1538 2209 2219
Fragments arranged by size:
2219 2209 1538 617
BsaAI yAC'GTr
Cuts at: 493 1204 2112 2773 4374 493
Size: 711 908 661 1601 2702
Fragments arranged by size:
2702 1601 908 711 661
BsaBI GATnn'nnATC
Cuts at: 3587 6577 3587
Size: 2990 3593
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2709 2907 3489 3558
Size: 53 83 186 936 1176 198 582 69
Cuts at: 3558 3730 4390 4772 5407 275
Size: 172 660 382 635 1451
Fragments arranged by size:
1451 1176 936 660 635 582 382 198
186 172 83 69 53
BsaWI w'CCGG_w
Cuts at: 5016 5414 5688 5835 6246 5016
Size: 398 274 147 411 5353
Fragments arranged by size:
5353 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3371 3401 1178
Size: 30 165 30 1968 30 4360
Fragments arranged by size:
4360 1968 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2734 2897 3239 3760 3863 1186
Size: 1548 163 342 521 103 3906
Fragments arranged by size:
3906 1548 521 342 163 103
BseYI C'CCAG_C
Cuts at: 2335 2527 2542 2560 2647 3641 3754 5786 6282
Size: 192 15 18 87 994 113 2032 496
Cuts at: 6282 2335
Size: 2636
Fragments arranged by size:
2636 2032 994 496 192 113 87 18
15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2287 2594 2615 3308 3451 1163
Size: 573 551 307 21 693 143 4295
Fragments arranged by size:
4295 693 573 551 307 143 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3147 4033 4794 5822 1540
Size: 275 186 1146 886 761 1028 2301
Fragments arranged by size:
2301 1146 1028 886 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2400 3047 3249 3414 4644 4729 5800 2400
Size: 647 202 165 1230 85 1071 3183
Fragments arranged by size:
3183 1230 1071 647 202 165 85
BsmI GAATG_Cn'
Cuts at: 2935 4094 4187 2935
Size: 1159 93 5331
Fragments arranged by size:
5331 1159 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2122 3020 2122
Size: 898 5685
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2400 2442 2553 2588 2622 2768 2964
Size: 399 610 42 111 35 34 146 196
Cuts at: 2964 3047 3249 3414 3523 3641 3706 3774 3902
Size: 83 202 165 109 118 65 68 128
Cuts at: 3902 4644 4729 5800 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 610 399 202 196 165
146 128 118 111 109 85 83 68
65 42 35 34
BspEI T'CCGG_A
Cuts at: 6246 6246
Size: 6583
BspHI T'CATG_A
Cuts at: 4472 6202 4472
Size: 1730 4853
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2587 3938 844
Size: 1743 1351 3489
Fragments arranged by size:
3489 1743 1351
BsrBI CCG'CTC
Cuts at: 1394 1823 3376 4470 1394
Size: 429 1553 1094 3507
Fragments arranged by size:
3507 1553 1094 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3358 5078 5252 65
Size: 1183 2110 1720 174 1396
Fragments arranged by size:
2110 1720 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2565 3142 5227 5414 1700
Size: 110 755 577 2085 187 2869
Fragments arranged by size:
2869 2085 755 577 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6583
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6583
BssSI C'ACGA_G
Cuts at: 2249 2534 2623 3407 4643 5655 2249
Size: 285 89 784 1236 1012 3177
Fragments arranged by size:
3177 1236 1012 784 285 89
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2617 794
Size: 1823 4760
BstBI TT'CG_AA
Cuts at: 2103 5387 2103
Size: 3284 3299
BstEII G'GTnAC_C
Cuts at: 1184 2887 1184
Size: 1703 4880
BstXI CCAn_nnnn'nTGG
Cuts at: 2434 3499 2434
Size: 1065 5518
BstYI r'GATC_y
Cuts at: 1972 3096 3737 3913 4280 4665 4682 6134 6220
Size: 1124 641 176 367 385 17 1452 86
Cuts at: 6220 6232 6578 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1124 641 385 367 346 176
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2356 2955 3105 3327 3561 513
Size: 552 1291 599 150 222 234 3535
Fragments arranged by size:
3535 1291 599 552 234 222 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3330 487
Size: 683 56 2104 3740
Fragments arranged by size:
3740 2104 683 56
BtsI GCAGTG_nn'
Cuts at: 2021 2603 3262 3296 4099 4893 4913 2021
Size: 582 659 34 803 794 20 3691
Fragments arranged by size:
3691 803 794 659 582 34 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6583
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6548
DraI TTT'AAA
Cuts at: 3887 3887
Size: 6583
DraIII CAC_nnn'GTG
Cuts at: 2617 2617
Size: 6583
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2837 5590 809
Size: 507 1521 2753 1802
Fragments arranged by size:
2753 1802 1521 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2638 2856 2922
Size: 54 1425 50 275 18 808 218 66
Cuts at: 2922 2938 3012 3144 3199 4030 4919 6548 8
Size: 16 74 132 55 831 889 1629 43
Fragments arranged by size:
1629 1425 889 831 808 275 218 132
74 66 55 54 50 43 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 3144 4030 1537
Size: 275 1332 886 4090
Fragments arranged by size:
4090 1332 886 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4513 1353
Size: 3160 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5170 5544 5690 387
Size: 1064 138 3581 374 146 1280
Fragments arranged by size:
3581 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2894 2900 3155 3275 3521 4646 5243
Size: 393 1262 6 255 120 246 1125 597
Cuts at: 5243 6030 6298 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1262 1125 787 597 393 268 255
246 120 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2846 2846
Size: 6583
EcoO109I rG'GnC_Cy
Cuts at: 2284 2362 3448 2284
Size: 78 1086 5419
Fragments arranged by size:
5419 1086 78
EcoRI G'AATT_C
Cuts at: 3892 4270 3892
Size: 378 6205
EcoRV GAT'ATC
Cuts at: 2413 2413
Size: 6583
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 6583
FspI TGC'GCA
Cuts at: 5089 5089
Size: 6583
HaeII r_GCGC'y
Cuts at: 1678 3492 3561 3733 5730 1678
Size: 1814 69 172 1997 2531
Fragments arranged by size:
2531 1997 1814 172 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3329 3361 5224 5256 5298
Size: 32 135 32 1757 32 1863 32 42
Cuts at: 5298 5330 1373
Size: 32 2626
Fragments arranged by size:
2626 1863 1757 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2419 3927 4173 669
Size: 988 281 481 1508 246 3079
Fragments arranged by size:
3079 1508 988 481 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6583
HpaI GTT'AAC
Cuts at: 4173 4173
Size: 6583
KasI G'GCGC_C
Cuts at: 3488 3557 3729 3488
Size: 69 172 6342
Fragments arranged by size:
6342 172 69
KpnI G_GTAC'C
Cuts at: 3908 3908
Size: 6583
MfeI C'AATT_G
Cuts at: 4182 4182
Size: 6583
MluI A'CGCG_T
Cuts at: 6459 6459
Size: 6583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3336 5697 5881 1305
Size: 2031 2361 184 2007
Fragments arranged by size:
2361 2031 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2940 3201 10
Size: 54 1425 343 1108 261 3392
Fragments arranged by size:
3392 1425 1108 343 261 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2070 2435 2465 2591 4541 4900 5059
Size: 648 904 365 30 126 1950 359 159
Cuts at: 5059 518
Size: 2042
Fragments arranged by size:
2042 1950 904 648 365 359 159 126
30
MspA1I CmG'CkG
Cuts at: 1606 2162 2478 2555 2812 3133 3402 3999 4676
Size: 556 316 77 257 321 269 597 677
Cuts at: 4676 5824 6069 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 597 556 321 316 269
257 245 77
NaeI GCC'GGC
Cuts at: 2567 3144 2567
Size: 577 6006
NarI GG'CG_CC
Cuts at: 3489 3558 3730 3489
Size: 69 172 6342
Fragments arranged by size:
6342 172 69
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6031
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6583
NgoMIV G'CCGG_C
Cuts at: 2565 3142 2565
Size: 577 6006
NheI G'CTAG_C
Cuts at: 1052 3853 1052
Size: 2801 3782
NotI GC'GGCC_GC
Cuts at: 4030 4030
Size: 6583
NspI r_CATG'y
Cuts at: 1132 1229 3941 5486 1132
Size: 97 2712 1545 2229
Fragments arranged by size:
2712 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6583
PciI A'CATG_T
Cuts at: 5482 5482
Size: 6583
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2647 1108
Size: 1539 5044
PfoI T'CCnGG_A
Cuts at: 2142 2406 2844 2871 3252 2142
Size: 264 438 27 381 5473
Fragments arranged by size:
5473 438 381 264 27
PmeI GTTT'AAAC
Cuts at: 3887 3887
Size: 6583
PmlI CAC'GTG
Cuts at: 2112 2773 2112
Size: 661 5922
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3365 3397 4652 4684 6165 6197 3365
Size: 32 1255 32 1481 32 3751
Fragments arranged by size:
3751 1481 1255 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2284 2362 2284
Size: 78 6505
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6583
PsiI TTA'TAA
Cuts at: 4153 4153
Size: 6583
PstI C_TGCA'G
Cuts at: 830 2685 3132 3935 830
Size: 1855 447 803 3478
Fragments arranged by size:
3478 1855 803 447
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6583
PvuII CAG'CTG
Cuts at: 2478 3133 2478
Size: 655 5928
SalI G'TCGA_C
Cuts at: 1936 3925 1936
Size: 1989 4594
SbfI CC_TGCA'GG
Cuts at: 3935 3935
Size: 6583
ScaI AGT'ACT
Cuts at: 2348 3173 4831 6543 2348
Size: 825 1658 1712 2388
Fragments arranged by size:
2388 1712 1658 825
SfcI C'TryA_G
Cuts at: 826 1045 2681 3128 3931 5066 5747 5938 826
Size: 219 1636 447 803 1135 681 191 1471
Fragments arranged by size:
1636 1471 1135 803 681 447 219 191
SfoI GGC'GCC
Cuts at: 3490 3559 3731 3490
Size: 69 172 6342
Fragments arranged by size:
6342 172 69
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6583
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6583
SmaI CCC'GGG
Cuts at: 1872 3910 1872
Size: 2038 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4687 5588 5850 820
Size: 197 926 2744 901 262 1553
Fragments arranged by size:
2744 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4374 493
Size: 3881 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6583
SphI G_CATG'C
Cuts at: 1132 3941 1132
Size: 2809 3774
SspI AAT'ATT
Cuts at: 5 52 4507 5
Size: 47 4455 2081
Fragments arranged by size:
4455 2081 47
StuI AGG'CCT
Cuts at: 1517 2660 1517
Size: 1143 5440
StyI C'CwwG_G
Cuts at: 513 1065 3825 4022 513
Size: 552 2760 197 3074
Fragments arranged by size:
3074 2760 552 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2252 2581 4610 4627 4780 4965 5410
Size: 1255 672 329 2029 17 153 185 445
Cuts at: 5410 325
Size: 1498
Fragments arranged by size:
2029 1498 1255 672 445 329 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2346 2382 3171
Size: 274 80 33 51 1443 369 36 789
Cuts at: 3171 3387 4829 6541 96
Size: 216 1442 1712 138
Fragments arranged by size:
1712 1443 1442 789 369 274 216 138
80 51 36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6543 6575 6543
Size: 32 6551
Tth111I GACn'n_nGTC
Cuts at: 1316 2906 1316
Size: 1590 4993
XbaI T'CTAG_A
Cuts at: 3919 3919
Size: 6583
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6583
XmaI C'CCGG_G
Cuts at: 1870 3908 1870
Size: 2038 4545
XmnI GAAnn'nnTTC
Cuts at: 4712 4712
Size: 6583
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2710 4391 276
Size: 53 83 186 936 1176 1681 2468
Fragments arranged by size:
2468 1681 1176 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BsiWI BstZ17I Bsu36I EcoICRI FseI FspAI NruI NsiI
PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI XcmI