Restriction map for
pFN21AB8375
(Circular) (Six-base) MAPSORT of: pFN21AB8375.seq Check: 9536 from: 1 to: 6682
pFN21AB8375 6682 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2691 4492 278
Size: 53 83 186 936 1155 1801 2468
Fragments arranged by size:
2468 1801 1155 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4025 1937
Size: 2088 4594
Acc65I G'GTAC_C
Cuts at: 4003 4003
Size: 6682
AclI AA'CG_TT
Cuts at: 1563 4809 5182 1563
Size: 3246 373 3063
Fragments arranged by size:
3246 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2475 3056 3737 4745 6129 6397 1632
Size: 843 581 681 1008 1384 268 1917
Fragments arranged by size:
1917 1384 1008 843 681 581 268
AfeI AGC'GCT
Cuts at: 2927 2956 3253 3653 2927
Size: 29 297 400 5956
Fragments arranged by size:
5956 400 297 29
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6485
AflIII A'CryG_T
Cuts at: 1203 2258 5581 6558 1203
Size: 1055 3323 977 1327
Fragments arranged by size:
3323 1327 1055 977
AgeI A'CCGG_T
Cuts at: 5513 5513
Size: 6682
AhdI GACnn_n'nnGTC
Cuts at: 5411 5411
Size: 6682
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6682
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6682
AlwNI CAG_nnn'CTG
Cuts at: 3034 4425 5997 3034
Size: 1391 1572 3719
Fragments arranged by size:
3719 1572 1391
ApaI G_GGCC'C
Cuts at: 2133 2133
Size: 6682
ApaLI G'TGCA_C
Cuts at: 4739 5895 4739
Size: 1156 5526
ApoI r'AATT_y
Cuts at: 1493 2240 3991 4217 4369 6478 1493
Size: 747 1751 226 152 2109 1697
Fragments arranged by size:
2109 1751 1697 747 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5236 160
Size: 872 4204 1606
Fragments arranged by size:
4204 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6682
AvaI C'yCGr_G
Cuts at: 1870 1943 2049 2565 3149 4007 1870
Size: 73 106 516 584 858 4545
Fragments arranged by size:
4545 858 584 516 106 73
BamHI G'GATC_C
Cuts at: 3285 4012 4379 3285
Size: 727 367 5588
Fragments arranged by size:
5588 727 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2123 2524 2935 3839 4003
Size: 325 217 627 336 401 411 904 164
Cuts at: 4003 5458 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 904 627 411 401 336 325
217 164
BanII G_rGCy'C
Cuts at: 1391 2068 2133 2535 3207 3321 3585 4001 1391
Size: 677 65 402 672 114 264 416 4072
Fragments arranged by size:
4072 677 672 416 402 264 114 65
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2980 3470 928
Size: 971 1081 490 4140
Fragments arranged by size:
4140 1081 971 490
BbvCI CC'TCA_GC
Cuts at: 3016 3016
Size: 6682
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2168 2202 4873 4907 1903
Size: 34 50 34 147 34 2671 34 3678
Fragments arranged by size:
3678 2671 147 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4574 5784 4574
Size: 1210 5472
BclI T'GATC_A
Cuts at: 1548 2268 1548
Size: 720 5962
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3794 5293 136
Size: 107 122 71 631 605 2122 1499 1525
Fragments arranged by size:
2122 1525 1499 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2448 2537 6677 2448
Size: 89 4140 2453
Fragments arranged by size:
4140 2453 89
BlpI GC'TnA_GC
Cuts at: 2027 2945 3356 4099 2027
Size: 918 411 743 4610
Fragments arranged by size:
4610 918 743 411
Bme1580I G_kGCm'C
Cuts at: 1790 2133 3700 3842 4743 5899 1790
Size: 343 1567 142 901 1156 2573
Fragments arranged by size:
2573 1567 1156 901 343 142
BmgBI CAC'GTC
Cuts at: 1318 2608 2820 3328 1318
Size: 1290 212 508 4672
Fragments arranged by size:
4672 1290 508 212
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2199 3688 4413
Size: 416 53 83 399 24 782 1489 725
Cuts at: 4413 5371 442
Size: 958 1753
Fragments arranged by size:
1753 1489 958 782 725 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6682
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2073 3260 5342 1239
Size: 834 1187 2082 2579
Fragments arranged by size:
2579 2082 1187 834
Bpu10I CC'TnA_GC
Cuts at: 3016 3299 3016
Size: 283 6399
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2834 3632 3791 4807 5672 5970 2834
Size: 798 159 1016 865 298 3546
Fragments arranged by size:
3546 1016 865 798 298 159
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2412 3436 3920 5345 882
Size: 617 913 1024 484 1425 2219
Fragments arranged by size:
2219 1425 1024 913 617 484
BsaAI yAC'GTr
Cuts at: 493 1204 2153 2968 4473 493
Size: 711 949 815 1505 2702
Fragments arranged by size:
2702 1505 949 815 711
BsaBI GATnn'nnATC
Cuts at: 6676 6676
Size: 6682
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2688 4489 4871 5506
Size: 53 83 186 936 1155 1801 382 635
Cuts at: 5506 275
Size: 1451
Fragments arranged by size:
1801 1451 1155 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3523 5115 5513 5787 5934 6345 3523
Size: 1592 398 274 147 411 3860
Fragments arranged by size:
3860 1592 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2543 2573 3372 3402 3614
Size: 30 165 30 1140 30 799 30 212
Cuts at: 3614 3644 1178
Size: 30 4216
Fragments arranged by size:
4216 1140 799 212 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2387 3329 3401 3749 1186
Size: 1201 942 72 348 4119
Fragments arranged by size:
4119 1201 942 348 72
BseYI C'CCAG_C
Cuts at: 2716 3101 3507 3556 3626 3789 3965 5885 6381
Size: 385 406 49 70 163 176 1920 496
Cuts at: 6381 2716
Size: 3017
Fragments arranged by size:
3017 1920 496 406 385 176 163 70
49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2255 2762 2764 2876 1163
Size: 573 519 507 2 112 4969
Fragments arranged by size:
4969 573 519 507 112 2
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2439 3109 3265 3404 3720 4132
Size: 275 186 438 670 156 139 316 412
Cuts at: 4132 4893 5921 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 670 438 412 316 275
186 156 139
BsiHKAI G_wGCw'C
Cuts at: 2058 3321 4743 4828 5899 2058
Size: 1263 1422 85 1071 2841
Fragments arranged by size:
2841 1422 1263 1071 85
BsmI GAATG_Cn'
Cuts at: 2286 3567 4193 4286 2286
Size: 1281 626 93 4682
Fragments arranged by size:
4682 1281 626 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2022 2159 2810 3083 3733 2022
Size: 137 651 273 650 4971
Fragments arranged by size:
4971 651 650 273 137
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2068 2133 2535 3207 3321 3585
Size: 399 268 10 65 402 672 114 264
Cuts at: 3585 3700 3842 4001 4743 4828 5899 1391
Size: 115 142 159 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 672 402 399 268 264
159 142 115 114 85 65 10
BspEI T'CCGG_A
Cuts at: 6345 6345
Size: 6682
BspHI T'CATG_A
Cuts at: 4571 6301 4571
Size: 1730 4952
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2786 4037 844
Size: 1942 1251 3489
Fragments arranged by size:
3489 1942 1251
BsrBI CCG'CTC
Cuts at: 1394 1823 2043 3283 4569 1394
Size: 429 220 1240 1286 3507
Fragments arranged by size:
3507 1286 1240 429 220
BsrDI GCAATG_nn'
Cuts at: 65 1248 5177 5351 65
Size: 1183 3929 174 1396
Fragments arranged by size:
3929 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2993 5326 5513 1700
Size: 110 1183 2333 187 2869
Fragments arranged by size:
2869 2333 1183 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6682
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6682
BssSI C'ACGA_G
Cuts at: 2867 3693 4742 5754 2867
Size: 826 1049 1012 3795
Fragments arranged by size:
3795 1049 1012 826
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2555 794
Size: 1761 4921
BstBI TT'CG_AA
Cuts at: 5486 5486
Size: 6682
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6682
BstXI CCAn_nnnn'nTGG
Cuts at: 2728 3185 2728
Size: 457 6225
BstYI r'GATC_y
Cuts at: 1972 2448 2537 2575 3285 4012 4379 4764 4781
Size: 476 89 38 710 727 367 385 17
Cuts at: 4781 6233 6319 6331 6677 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 727 710 476 385 367 346
89 86 38 17 12
Bsu36I CC'TnA_GG
Cuts at: 2413 2413
Size: 6682
BtgI C'CryG_G
Cuts at: 513 1065 2439 2841 513
Size: 552 1374 402 4354
Fragments arranged by size:
4354 1374 552 402
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2126 487
Size: 683 56 900 5043
Fragments arranged by size:
5043 900 683 56
BtsI GCAGTG_nn'
Cuts at: 3370 4198 4992 5012 3370
Size: 828 794 20 5040
Fragments arranged by size:
5040 828 794 20
ClaI AT'CG_AT
Cuts at: 1554 2694 1554
Size: 1140 5542
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6647
DraI TTT'AAA
Cuts at: 3986 3986
Size: 6682
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3428 5689 809
Size: 507 2112 2261 1802
Fragments arranged by size:
2261 2112 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2436 2995 3106
Size: 54 1425 50 275 18 606 559 111
Cuts at: 3106 3262 3701 3717 4129 5018 6647 8
Size: 156 439 16 412 889 1629 43
Fragments arranged by size:
1629 1425 889 606 559 439 412 275
156 111 54 50 43 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 2436 3106 3262 3717 4129 1537
Size: 275 624 670 156 455 412 4090
Fragments arranged by size:
4090 670 624 455 412 275 156
EarI CTCTTCn'nnn_
Cuts at: 1353 4612 1353
Size: 3259 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2035 5269 5643 5789 387
Size: 1064 138 446 3234 374 146 1280
Fragments arranged by size:
3234 1280 1064 446 374 146 138
EcoICRI GAG'CTC
Cuts at: 3319 3319
Size: 6682
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2073 2475 3056 3260 3737 4745 5342
Size: 393 441 402 581 204 477 1008 597
Cuts at: 5342 6129 6397 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1008 787 597 581 477 441 402
393 268 204
EcoO109I rG'GnC_Cy
Cuts at: 2130 2251 3060 3295 3683 2130
Size: 121 809 235 388 5129
Fragments arranged by size:
5129 809 388 235 121
EcoRI G'AATT_C
Cuts at: 2240 3991 4369 2240
Size: 1751 378 4553
Fragments arranged by size:
4553 1751 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2052 2052
Size: 6682
FspI TGC'GCA
Cuts at: 5188 5188
Size: 6682
HaeII r_GCGC'y
Cuts at: 1678 2929 2958 3035 3255 3655 5829 1678
Size: 1251 29 77 220 400 2174 2531
Fragments arranged by size:
2531 2174 1251 400 220 77 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2069 2101 3527 3559 5323
Size: 32 135 32 497 32 1426 32 1764
Cuts at: 5323 5355 5397 5429 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1764 1426 497 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3848 4026 4272 669
Size: 988 281 1910 178 246 3079
Fragments arranged by size:
3079 1910 988 281 246 178
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6682
HpaI GTT'AAC
Cuts at: 4272 4272
Size: 6682
KpnI G_GTAC'C
Cuts at: 4007 4007
Size: 6682
MfeI C'AATT_G
Cuts at: 4281 4281
Size: 6682
MluI A'CGCG_T
Cuts at: 6558 6558
Size: 6682
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2655 3957 5796 5980 1305
Size: 1350 1302 1839 184 2007
Fragments arranged by size:
2007 1839 1350 1302 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4860
Fragments arranged by size:
4860 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2786 2792 4640 4999 5158 518
Size: 648 1620 6 1848 359 159 2042
Fragments arranged by size:
2042 1848 1620 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2435 2738 2811 2941 3105 3355 3793 3965
Size: 829 303 73 130 164 250 438 172
Cuts at: 3965 4098 4775 5923 6168 1606
Size: 133 677 1148 245 2120
Fragments arranged by size:
2120 1148 829 677 438 303 250 245
172 164 133 130 73
NcoI C'CATG_G
Cuts at: 513 1065 2841 513
Size: 552 1776 4354
Fragments arranged by size:
4354 1776 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6682
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6682
NotI GC'GGCC_GC
Cuts at: 4129 4129
Size: 6682
NspI r_CATG'y
Cuts at: 1132 1229 2262 2471 4040 5585 1132
Size: 97 1033 209 1569 1545 2229
Fragments arranged by size:
2229 1569 1545 1033 209 97
PasI CC'CwG_GG
Cuts at: 1348 3891 1348
Size: 2543 4139
PciI A'CATG_T
Cuts at: 2258 5581 2258
Size: 3323 3359
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2872 3049 3217 1108
Size: 1764 177 168 4573
Fragments arranged by size:
4573 1764 177 168
PfoI T'CCnGG_A
Cuts at: 2160 2323 2727 3237 3321 3609 2160
Size: 163 404 510 84 288 5233
Fragments arranged by size:
5233 510 404 288 163 84
PmeI GTTT'AAAC
Cuts at: 3986 3986
Size: 6682
PmlI CAC'GTG
Cuts at: 2153 2153
Size: 6682
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4751 4783 6264 6296 4751
Size: 32 1481 32 5137
Fragments arranged by size:
5137 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2251 3295 3683 2251
Size: 1044 388 5250
Fragments arranged by size:
5250 1044 388
PshAI GACnn'nnGTC
Cuts at: 1613 2171 1613
Size: 558 6124
PsiI TTA'TAA
Cuts at: 2226 4252 2226
Size: 2026 4656
PspOMI G'GGCC_C
Cuts at: 2129 2129
Size: 6682
PstI C_TGCA'G
Cuts at: 830 3127 3396 3594 4034 830
Size: 2297 269 198 440 3478
Fragments arranged by size:
3478 2297 440 269 198
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6682
PvuII CAG'CTG
Cuts at: 2738 3355 2738
Size: 617 6065
SacI G_AGCT'C
Cuts at: 3321 3321
Size: 6682
SalI G'TCGA_C
Cuts at: 1936 4024 1936
Size: 2088 4594
SanDI GG'GwC_CC
Cuts at: 3683 3683
Size: 6682
SbfI CC_TGCA'GG
Cuts at: 4034 4034
Size: 6682
ScaI AGT'ACT
Cuts at: 4930 6642 4930
Size: 1712 4970
SexAI A'CCwGG_T
Cuts at: 2616 2616
Size: 6682
SfcI C'TryA_G
Cuts at: 826 1045 2824 3123 3392 3590 4030 5165 5846
Size: 219 1779 299 269 198 440 1135 681
Cuts at: 5846 6037 826
Size: 191 1471
Fragments arranged by size:
1779 1471 1135 681 440 299 269 219
198 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6682
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6682
SmaI CCC'GGG
Cuts at: 1872 2051 2567 4009 1872
Size: 179 516 1442 4545
Fragments arranged by size:
4545 1442 516 179
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2849 3647 3806 4786 5687 5949
Size: 197 926 906 798 159 980 901 262
Cuts at: 5949 820
Size: 1553
Fragments arranged by size:
1553 980 926 906 901 798 262 197
159
SnaBI TAC'GTA
Cuts at: 493 4473 493
Size: 3980 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6682
SphI G_CATG'C
Cuts at: 1132 4040 1132
Size: 2908 3774
SspI AAT'ATT
Cuts at: 5 52 4606 5
Size: 47 4554 2081
Fragments arranged by size:
4554 2081 47
StuI AGG'CCT
Cuts at: 1517 2463 2481 2496 3014 1517
Size: 946 18 15 518 5185
Fragments arranged by size:
5185 946 518 18 15
StyI C'CwwG_G
Cuts at: 513 1065 2841 3003 4121 513
Size: 552 1776 162 1118 3074
Fragments arranged by size:
3074 1776 1118 552 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3904 4709 4726 4879 5064 5509 325
Size: 1255 2324 805 17 153 185 445 1498
Fragments arranged by size:
2324 1498 1255 805 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3336 4928 6640
Size: 274 80 33 51 1443 1359 1592 1712
Cuts at: 6640 96
Size: 138
Fragments arranged by size:
1712 1592 1443 1359 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2142 2174 3372 3404 6642 6674 2142
Size: 32 1198 32 3238 32 2150
Fragments arranged by size:
3238 2150 1198 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6682
XbaI T'CTAG_A
Cuts at: 2060 4018 2060
Size: 1958 4724
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2569 3214 3944 2569
Size: 645 730 5307
Fragments arranged by size:
5307 730 645
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6682
XmaI C'CCGG_G
Cuts at: 1870 2049 2565 4007 1870
Size: 179 516 1442 4545
Fragments arranged by size:
4545 1442 516 179
XmnI GAAnn'nnTTC
Cuts at: 4811 4811
Size: 6682
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2689 4490 276
Size: 53 83 186 936 1155 1801 2468
Fragments arranged by size:
2468 1801 1155 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI
RsrII SacII SapI SfiI SfoI SrfI SwaI