Restriction map for
pFN21AB8372
(Circular) (Six-base) MAPSORT of: pFN21AB8372.seq Check: 1543 from: 1 to: 5200
pFN21AB8372 5200 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3010 278
Size: 53 83 186 936 1474 2468
Fragments arranged by size:
2468 1474 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2151 2543 1937
Size: 214 392 4594
Fragments arranged by size:
4594 392 214
Acc65I G'GTAC_C
Cuts at: 2521 2521
Size: 5200
AclI AA'CG_TT
Cuts at: 1563 3327 3700 1563
Size: 1764 373 3063
Fragments arranged by size:
3063 1764 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3263 4647 4915 1632
Size: 1631 1384 268 1917
Fragments arranged by size:
1917 1631 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2422 820
Size: 197 1405 3598
Fragments arranged by size:
3598 1405 197
AflIII A'CryG_T
Cuts at: 1203 4099 5076 1203
Size: 2896 977 1327
Fragments arranged by size:
2896 1327 977
AgeI A'CCGG_T
Cuts at: 4031 4031
Size: 5200
AhdI GACnn_n'nnGTC
Cuts at: 3929 3929
Size: 5200
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5200
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5200
AlwNI CAG_nnn'CTG
Cuts at: 2943 4515 2943
Size: 1572 3628
ApaLI G'TGCA_C
Cuts at: 3257 4413 3257
Size: 1156 4044
ApoI r'AATT_y
Cuts at: 1493 2018 2509 2735 2887 4996 1493
Size: 525 491 226 152 2109 1697
Fragments arranged by size:
2109 1697 525 491 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2484 3754 160
Size: 872 1452 1270 1606
Fragments arranged by size:
1606 1452 1270 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5200
AvaI C'yCGr_G
Cuts at: 1870 1943 2287 2525 1870
Size: 73 344 238 4545
Fragments arranged by size:
4545 344 238 73
BamHI G'GATC_C
Cuts at: 2413 2530 2897 2413
Size: 117 367 4716
Fragments arranged by size:
4716 367 117
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2348 2521 3976 618
Size: 325 217 627 561 173 1455 1842
Fragments arranged by size:
1842 1455 627 561 325 217 173
BanII G_rGCy'C
Cuts at: 1391 2307 2519 1391
Size: 916 212 4072
Fragments arranged by size:
4072 916 212
BbeI G_GCGC'C
Cuts at: 2352 2352
Size: 5200
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2045 928
Size: 971 146 4083
Fragments arranged by size:
4083 971 146
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3391 3425 1903
Size: 34 1454 34 3678
Fragments arranged by size:
3678 1454 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3092 4302 3092
Size: 1210 3990
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5200
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3811 136
Size: 107 122 71 631 605 2139 1525
Fragments arranged by size:
2139 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5195 5195
Size: 5200
BlpI GC'TnA_GC
Cuts at: 2084 2617 2084
Size: 533 4667
Bme1580I G_kGCm'C
Cuts at: 1790 3261 4417 1790
Size: 1471 1156 2573
Fragments arranged by size:
2573 1471 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5200
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2931 3889 442
Size: 416 53 83 399 24 1514 958 1753
Fragments arranged by size:
1753 1514 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5200
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2038 2038
Size: 5200
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2049 2093 3860 1239
Size: 810 44 1767 2579
Fragments arranged by size:
2579 1767 810 44
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3325 4190 4488 3325
Size: 865 298 4037
Fragments arranged by size:
4037 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2312 3863 882
Size: 617 813 1551 2219
Fragments arranged by size:
2219 1551 813 617
BsaAI yAC'GTr
Cuts at: 493 1204 2991 493
Size: 711 1787 2702
Fragments arranged by size:
2702 1787 711
BsaBI GATnn'nnATC
Cuts at: 5194 5194
Size: 5200
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2349 3007 3389 4024
Size: 53 83 186 936 816 658 382 635
Cuts at: 4024 275
Size: 1451
Fragments arranged by size:
1451 936 816 658 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3633 4031 4305 4452 4863 3633
Size: 398 274 147 411 3970
Fragments arranged by size:
3970 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4975
Fragments arranged by size:
4975 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5200
BseYI C'CCAG_C
Cuts at: 4403 4899 4403
Size: 496 4704
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2354 1163
Size: 573 618 4009
Fragments arranged by size:
4009 618 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2650 3411 4439 1540
Size: 275 186 649 761 1028 2301
Fragments arranged by size:
2301 1028 761 649 275 186
BsiHKAI G_wGCw'C
Cuts at: 2085 2307 3261 3346 4417 2085
Size: 222 954 85 1071 2868
Fragments arranged by size:
2868 1071 954 222 85
BsmI GAATG_Cn'
Cuts at: 2711 2804 2711
Size: 93 5107
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 2307 2519 3261 3346 4417 1391
Size: 399 295 222 212 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 295 222 212 85
BspEI T'CCGG_A
Cuts at: 4863 4863
Size: 5200
BspHI T'CATG_A
Cuts at: 3089 4819 3089
Size: 1730 3470
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2376 2555 844
Size: 1532 179 3489
Fragments arranged by size:
3489 1532 179
BsrBI CCG'CTC
Cuts at: 1394 1823 3087 1394
Size: 429 1264 3507
Fragments arranged by size:
3507 1264 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3695 3869 65
Size: 1183 2447 174 1396
Fragments arranged by size:
2447 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2292 3844 4031 1700
Size: 110 482 1552 187 2869
Fragments arranged by size:
2869 1552 482 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5200
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5200
BssSI C'ACGA_G
Cuts at: 3260 4272 3260
Size: 1012 4188
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2377 794
Size: 1583 3617
BstBI TT'CG_AA
Cuts at: 4004 4004
Size: 5200
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5200
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5200
BstYI r'GATC_y
Cuts at: 1972 2220 2413 2530 2897 3282 3299 4751 4837
Size: 248 193 117 367 385 17 1452 86
Cuts at: 4837 4849 5195 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 248 193 117
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2334 513
Size: 552 1269 3379
Fragments arranged by size:
3379 1269 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4461
Fragments arranged by size:
4461 683 56
BtsI GCAGTG_nn'
Cuts at: 2371 2716 3510 3530 2371
Size: 345 794 20 4041
Fragments arranged by size:
4041 794 345 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5200
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5165
DraI TTT'AAA
Cuts at: 2504 2504
Size: 5200
DraIII CAC_nnn'GTG
Cuts at: 2377 2377
Size: 5200
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4207 809
Size: 507 2891 1802
Fragments arranged by size:
2891 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 2647 3536
Size: 54 1425 50 275 18 303 514 889
Cuts at: 3536 5165 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 514 303 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2647 1537
Size: 275 835 4090
Fragments arranged by size:
4090 835 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3130 1353
Size: 1777 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2201 2315 3787 4161 4307 387
Size: 1064 138 612 114 1472 374 146 1280
Fragments arranged by size:
1472 1280 1064 612 374 146 138 114
EcoICRI GAG'CTC
Cuts at: 2305 2305
Size: 5200
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2049 2093 3263 3860 4647 4915 1239
Size: 393 417 44 1170 597 787 268 1524
Fragments arranged by size:
1524 1170 787 597 417 393 268 44
EcoNI CCTnn'n_nnAGG
Cuts at: 2237 2237
Size: 5200
EcoRI G'AATT_C
Cuts at: 2018 2509 2887 2018
Size: 491 378 4331
Fragments arranged by size:
4331 491 378
FspI TGC'GCA
Cuts at: 3706 3706
Size: 5200
HaeII r_GCGC'y
Cuts at: 1678 2352 4347 1678
Size: 674 1995 2531
Fragments arranged by size:
2531 1995 674
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3841 3873 3915 3947 1373
Size: 32 135 32 2269 32 42 32 2626
Fragments arranged by size:
2626 2269 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2152 2429 2544 2790 669
Size: 988 281 214 277 115 246 3079
Fragments arranged by size:
3079 988 281 277 246 214 115
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5200
HpaI GTT'AAC
Cuts at: 2790 2790
Size: 5200
KasI G'GCGC_C
Cuts at: 2348 2348
Size: 5200
KpnI G_GTAC'C
Cuts at: 2525 2525
Size: 5200
MfeI C'AATT_G
Cuts at: 2799 2799
Size: 5200
MluI A'CGCG_T
Cuts at: 5076 5076
Size: 5200
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4314 4498 1305
Size: 3009 184 2007
Fragments arranged by size:
3009 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3378
Fragments arranged by size:
3378 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2404 3158 3517 3676 518
Size: 648 1238 754 359 159 2042
Fragments arranged by size:
2042 1238 754 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2336 2616 3293 4441 4686 1606
Size: 730 280 677 1148 245 2120
Fragments arranged by size:
2120 1148 730 677 280 245
NaeI GCC'GGC
Cuts at: 2294 2294
Size: 5200
NarI GG'CG_CC
Cuts at: 2349 2349
Size: 5200
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4648
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5200
NgoMIV G'CCGG_C
Cuts at: 2292 2292
Size: 5200
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5200
NotI GC'GGCC_GC
Cuts at: 2647 2647
Size: 5200
NspI r_CATG'y
Cuts at: 1132 1229 2558 4103 1132
Size: 97 1329 1545 2229
Fragments arranged by size:
2229 1545 1329 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5200
PciI A'CATG_T
Cuts at: 4099 4099
Size: 5200
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5200
PmeI GTTT'AAAC
Cuts at: 2504 2504
Size: 5200
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3269 3301 4782 4814 3269
Size: 32 1481 32 3655
Fragments arranged by size:
3655 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5200
PsiI TTA'TAA
Cuts at: 2770 2770
Size: 5200
PstI C_TGCA'G
Cuts at: 830 2220 2552 830
Size: 1390 332 3478
Fragments arranged by size:
3478 1390 332
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5200
SacI G_AGCT'C
Cuts at: 2307 2307
Size: 5200
SacII CC_GC'GG
Cuts at: 2337 2337
Size: 5200
SalI G'TCGA_C
Cuts at: 1936 2150 2542 1936
Size: 214 392 4594
Fragments arranged by size:
4594 392 214
SbfI CC_TGCA'GG
Cuts at: 2552 2552
Size: 5200
ScaI AGT'ACT
Cuts at: 3448 5160 3448
Size: 1712 3488
SfcI C'TryA_G
Cuts at: 826 1045 2216 2548 3683 4364 4555 826
Size: 219 1171 332 1135 681 191 1471
Fragments arranged by size:
1471 1171 1135 681 332 219 191
SfoI GGC'GCC
Cuts at: 2350 2350
Size: 5200
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5200
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5200
SmaI CCC'GGG
Cuts at: 1872 2289 2527 1872
Size: 417 238 4545
Fragments arranged by size:
4545 417 238
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2422 3304 4205 4467 820
Size: 197 926 479 882 901 262 1553
Fragments arranged by size:
1553 926 901 882 479 262 197
SnaBI TAC'GTA
Cuts at: 493 2991 493
Size: 2498 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5200
SphI G_CATG'C
Cuts at: 1132 2558 1132
Size: 1426 3774
SspI AAT'ATT
Cuts at: 5 52 3124 5
Size: 47 3072 2081
Fragments arranged by size:
3072 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5200
StyI C'CwwG_G
Cuts at: 513 1065 2639 513
Size: 552 1574 3074
Fragments arranged by size:
3074 1574 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2057 2336 3227 3244 3397 3582 4027
Size: 1255 477 279 891 17 153 185 445
Cuts at: 4027 325
Size: 1498
Fragments arranged by size:
1498 1255 891 477 445 279 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3446 5158 96
Size: 274 80 33 51 1443 1469 1712 138
Fragments arranged by size:
1712 1469 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5160 5192 5160
Size: 32 5168
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5200
XbaI T'CTAG_A
Cuts at: 2536 2536
Size: 5200
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5200
XmaI C'CCGG_G
Cuts at: 1870 2287 2525 1870
Size: 417 238 4545
Fragments arranged by size:
4545 417 238
XmnI GAAnn'nnTTC
Cuts at: 3329 3329
Size: 5200
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3008 276
Size: 53 83 186 936 1474 2468
Fragments arranged by size:
2468 1474 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SacI SacII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoO109I EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SanDI SapI
SexAI SfiI SrfI SwaI XcmI