Restriction map for
pFN21AB8371
(Circular) (Six-base) MAPSORT of: pFN21AB8371.seq Check: 2137 from: 1 to: 6262
pFN21AB8371 6262 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4072 278
Size: 53 83 186 936 2536 2468
Fragments arranged by size:
2536 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2964 3605 1937
Size: 1027 641 4594
Fragments arranged by size:
4594 1027 641
Acc65I G'GTAC_C
Cuts at: 3583 3583
Size: 6262
AclI AA'CG_TT
Cuts at: 1563 4389 4762 1563
Size: 2826 373 3063
Fragments arranged by size:
3063 2826 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3567 4325 5709 5977 1632
Size: 1935 758 1384 268 1917
Fragments arranged by size:
1935 1917 1384 758 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6065
AflIII A'CryG_T
Cuts at: 1203 2982 5161 6138 1203
Size: 1779 2179 977 1327
Fragments arranged by size:
2179 1779 1327 977
AgeI A'CCGG_T
Cuts at: 5093 5093
Size: 6262
AhdI GACnn_n'nnGTC
Cuts at: 4991 4991
Size: 6262
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6262
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6262
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2151 2183 2151
Size: 32 6230
AlwNI CAG_nnn'CTG
Cuts at: 4005 5577 4005
Size: 1572 4690
ApaI G_GGCC'C
Cuts at: 3514 3514
Size: 6262
ApaLI G'TGCA_C
Cuts at: 4319 5475 4319
Size: 1156 5106
ApoI r'AATT_y
Cuts at: 1493 2242 3571 3797 3949 6058 1493
Size: 749 1329 226 152 2109 1697
Fragments arranged by size:
2109 1697 1329 749 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4816 160
Size: 872 3784 1606
Fragments arranged by size:
3784 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6262
AvaI C'yCGr_G
Cuts at: 1870 1943 2025 3587 1870
Size: 73 82 1562 4545
Fragments arranged by size:
4545 1562 82 73
BamHI G'GATC_C
Cuts at: 3592 3959 3592
Size: 367 5895
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3583 5038 618
Size: 325 217 627 1796 1455 1842
Fragments arranged by size:
1842 1796 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3514 3581 1391
Size: 2123 67 4072
Fragments arranged by size:
4072 2123 67
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5291
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4453 4487 1903
Size: 34 2516 34 3678
Fragments arranged by size:
3678 2516 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4154 5364 4154
Size: 1210 5052
BclI T'GATC_A
Cuts at: 1548 2536 1548
Size: 988 5274
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4873 136
Size: 107 122 71 631 605 3201 1525
Fragments arranged by size:
3201 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6257 6257
Size: 6262
BlpI GC'TnA_GC
Cuts at: 3679 3679
Size: 6262
Bme1580I G_kGCm'C
Cuts at: 1790 3514 4323 5479 1790
Size: 1724 809 1156 2573
Fragments arranged by size:
2573 1724 1156 809
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6262
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3993 4951 442
Size: 416 53 83 399 24 2576 958 1753
Fragments arranged by size:
2576 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6262
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2541 3045 3432 4922 1239
Size: 1302 504 387 1490 2579
Fragments arranged by size:
2579 1490 1302 504 387
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2484 3076 4387 5252 5550 2484
Size: 592 1311 865 298 3196
Fragments arranged by size:
3196 1311 865 592 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4925 882
Size: 617 3426 2219
Fragments arranged by size:
3426 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4053 493
Size: 711 2849 2702
Fragments arranged by size:
2849 2702 711
BsaBI GATnn'nnATC
Cuts at: 6256 6256
Size: 6262
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4069 4451 5086 275
Size: 53 83 186 936 2536 382 635 1451
Fragments arranged by size:
2536 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4695 5093 5367 5514 5925 4695
Size: 398 274 147 411 5032
Fragments arranged by size:
5032 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2151 2181 2550 2580 1178
Size: 30 165 30 748 30 369 30 4860
Fragments arranged by size:
4860 748 369 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2852 1186
Size: 1666 4596
BseYI C'CCAG_C
Cuts at: 2131 2397 3248 5465 5961 2131
Size: 266 851 2217 496 2432
Fragments arranged by size:
2432 2217 851 496 266
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5689
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3712 4473 5501 1540
Size: 275 186 1711 761 1028 2301
Fragments arranged by size:
2301 1711 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4323 4408 5479 4323
Size: 85 1071 5106
Fragments arranged by size:
5106 1071 85
BsmI GAATG_Cn'
Cuts at: 3773 3866 3773
Size: 93 6169
BsmBI CGTCTCn'nnnn_
Cuts at: 2966 2966
Size: 6262
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3514 3581 4323 4408 5479 1391
Size: 399 1724 67 742 85 1071 2174
Fragments arranged by size:
2174 1724 1071 742 399 85 67
BspEI T'CCGG_A
Cuts at: 5925 5925
Size: 6262
BspHI T'CATG_A
Cuts at: 2905 4151 5881 2905
Size: 1246 1730 3286
Fragments arranged by size:
3286 1730 1246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2203 3617 844
Size: 1359 1414 3489
Fragments arranged by size:
3489 1414 1359
BsrBI CCG'CTC
Cuts at: 1394 1823 4149 1394
Size: 429 2326 3507
Fragments arranged by size:
3507 2326 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2761 4757 4931 65
Size: 1183 1513 1996 174 1396
Fragments arranged by size:
1996 1513 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4906 5093 1700
Size: 110 3096 187 2869
Fragments arranged by size:
3096 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2478 2985 96
Size: 2382 507 3373
Fragments arranged by size:
3373 2382 507
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6262
BssSI C'ACGA_G
Cuts at: 2417 4322 5334 2417
Size: 1905 1012 3345
Fragments arranged by size:
3345 1905 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6262
BstBI TT'CG_AA
Cuts at: 5066 5066
Size: 6262
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6262
BstXI CCAn_nnnn'nTGG
Cuts at: 3082 3341 3082
Size: 259 6003
BstYI r'GATC_y
Cuts at: 1972 2221 3592 3959 4344 4361 5813 5899 5911
Size: 249 1371 367 385 17 1452 86 12
Cuts at: 5911 6257 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1371 385 367 346 249 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5710
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5523
Fragments arranged by size:
5523 683 56
BtsI GCAGTG_nn'
Cuts at: 2295 3346 3778 4572 4592 2295
Size: 1051 432 794 20 3965
Fragments arranged by size:
3965 1051 794 432 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6262
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6227
DraI TTT'AAA
Cuts at: 3566 3566
Size: 6262
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5269 809
Size: 507 3953 1802
Fragments arranged by size:
3953 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3709 4598 6227
Size: 54 1425 50 275 18 1879 889 1629
Cuts at: 6227 8
Size: 43
Fragments arranged by size:
1879 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3709 1537
Size: 275 1897 4090
Fragments arranged by size:
4090 1897 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4192 1353
Size: 2839 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4849 5223 5369 387
Size: 1064 138 3260 374 146 1280
Fragments arranged by size:
3260 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2541 3045 3432 3567 4325 4922 5709
Size: 393 909 504 387 135 758 597 787
Cuts at: 5709 5977 1239
Size: 268 1524
Fragments arranged by size:
1524 909 787 758 597 504 393 387
268 135
EcoRI G'AATT_C
Cuts at: 2242 3571 3949 2242
Size: 1329 378 4555
Fragments arranged by size:
4555 1329 378
FspI TGC'GCA
Cuts at: 4768 4768
Size: 6262
HaeII r_GCGC'y
Cuts at: 1678 5409 1678
Size: 3731 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3178 3210 4903 4935 4977
Size: 32 135 32 1606 32 1693 32 42
Cuts at: 4977 5009 1373
Size: 32 2626
Fragments arranged by size:
2626 1693 1606 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2878 3606 3852 669
Size: 988 281 940 728 246 3079
Fragments arranged by size:
3079 988 940 728 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6262
HpaI GTT'AAC
Cuts at: 3852 3852
Size: 6262
KpnI G_GTAC'C
Cuts at: 3587 3587
Size: 6262
MfeI C'AATT_G
Cuts at: 3861 3861
Size: 6262
MluI A'CGCG_T
Cuts at: 6138 6138
Size: 6262
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5376 5560 1305
Size: 4071 184 2007
Fragments arranged by size:
4071 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4440
Fragments arranged by size:
4440 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2910 3180 3278 4220 4579 4738 518
Size: 648 1744 270 98 942 359 159 2042
Fragments arranged by size:
2042 1744 942 648 359 270 159 98
MspA1I CmG'CkG
Cuts at: 1606 3678 4355 5503 5748 1606
Size: 2072 677 1148 245 2120
Fragments arranged by size:
2120 2072 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5710
NdeI CA'TA_TG
Cuts at: 387 2208 387
Size: 1821 4441
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6262
NotI GC'GGCC_GC
Cuts at: 3709 3709
Size: 6262
NspI r_CATG'y
Cuts at: 1132 1229 2986 3620 5165 1132
Size: 97 1757 634 1545 2229
Fragments arranged by size:
2229 1757 1545 634 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6262
PciI A'CATG_T
Cuts at: 2982 5161 2982
Size: 2179 4083
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6262
PmeI GTTT'AAAC
Cuts at: 3566 3566
Size: 6262
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2151 2183 2508 2540 4331 4363 5844 5876 2151
Size: 32 325 32 1791 32 1481 32 2537
Fragments arranged by size:
2537 1791 1481 325 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2658 1613
Size: 1045 5217
PsiI TTA'TAA
Cuts at: 3832 3832
Size: 6262
PspOMI G'GGCC_C
Cuts at: 3510 3510
Size: 6262
PstI C_TGCA'G
Cuts at: 830 3143 3614 830
Size: 2313 471 3478
Fragments arranged by size:
3478 2313 471
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6262
SalI G'TCGA_C
Cuts at: 1936 3604 1936
Size: 1668 4594
SbfI CC_TGCA'GG
Cuts at: 3614 3614
Size: 6262
ScaI AGT'ACT
Cuts at: 4510 6222 4510
Size: 1712 4550
SexAI A'CCwGG_T
Cuts at: 2103 2103
Size: 6262
SfcI C'TryA_G
Cuts at: 826 1045 3108 3139 3610 4745 5426 5617 826
Size: 219 2063 31 471 1135 681 191 1471
Fragments arranged by size:
2063 1471 1135 681 471 219 191 31
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6262
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6262
SmaI CCC'GGG
Cuts at: 1872 3589 1872
Size: 1717 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2499 3091 4366 5267 5529 820
Size: 197 926 556 592 1275 901 262 1553
Fragments arranged by size:
1553 1275 926 901 592 556 262 197
SnaBI TAC'GTA
Cuts at: 493 4053 493
Size: 3560 2702
SpeI A'CTAG_T
Cuts at: 152 2257 152
Size: 2105 4157
SphI G_CATG'C
Cuts at: 1132 3620 1132
Size: 2488 3774
SspI AAT'ATT
Cuts at: 5 52 4186 5
Size: 47 4134 2081
Fragments arranged by size:
4134 2081 47
StuI AGG'CCT
Cuts at: 1517 3380 1517
Size: 1863 4399
StyI C'CwwG_G
Cuts at: 513 1065 2461 3701 513
Size: 552 1396 1240 3074
Fragments arranged by size:
3074 1396 1240 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4289 4306 4459 4644 5089 325
Size: 1255 2709 17 153 185 445 1498
Fragments arranged by size:
2709 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2478 2985 3518
Size: 274 80 33 51 1443 501 507 533
Cuts at: 3518 4508 6220 96
Size: 990 1712 138
Fragments arranged by size:
1712 1443 990 533 507 501 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2167 2199 3456 3488 6222 6254 2167
Size: 32 1257 32 2734 32 2175
Fragments arranged by size:
2734 2175 1257 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6262
XbaI T'CTAG_A
Cuts at: 3598 3598
Size: 6262
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6262
XmaI C'CCGG_G
Cuts at: 1870 3587 1870
Size: 1717 4545
XmnI GAAnn'nnTTC
Cuts at: 4391 4391
Size: 6262
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4070 276
Size: 53 83 186 936 2536 2468
Fragments arranged by size:
2536 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI XcmI