Restriction map for
pFN21AB8364
(Circular) (Six-base) MAPSORT of: pFN21AB8364.seq Check: 4288 from: 1 to: 6154
pFN21AB8364 6154 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3254 3254
Size: 6154
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3444 3964 278
Size: 53 83 186 936 1908 520 2468
Fragments arranged by size:
2468 1908 936 520 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3497 1937
Size: 1560 4594
Acc65I G'GTAC_C
Cuts at: 3475 3475
Size: 6154
AclI AA'CG_TT
Cuts at: 1563 4281 4654 1563
Size: 2718 373 3063
Fragments arranged by size:
3063 2718 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2047 2501 3343 4217 5601 5869 1632
Size: 415 454 842 874 1384 268 1917
Fragments arranged by size:
1917 1384 874 842 454 415 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5957
AflIII A'CryG_T
Cuts at: 1203 2418 3407 5053 6030 1203
Size: 1215 989 1646 977 1327
Fragments arranged by size:
1646 1327 1215 989 977
AgeI A'CCGG_T
Cuts at: 4985 4985
Size: 6154
AhdI GACnn_n'nnGTC
Cuts at: 4883 4883
Size: 6154
AleI CACnn'nnGTG
Cuts at: 1166 2732 1166
Size: 1566 4588
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6154
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2405 2437 2405
Size: 32 6122
AlwNI CAG_nnn'CTG
Cuts at: 2218 2515 2878 3133 3897 5469 2218
Size: 297 363 255 764 1572 2903
Fragments arranged by size:
2903 1572 764 363 297 255
ApaI G_GGCC'C
Cuts at: 2091 2091
Size: 6154
ApaLI G'TGCA_C
Cuts at: 4211 5367 4211
Size: 1156 4998
ApoI r'AATT_y
Cuts at: 1493 2579 3463 3689 3841 5950 1493
Size: 1086 884 226 152 2109 1697
Fragments arranged by size:
2109 1697 1086 884 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4708 160
Size: 872 3676 1606
Fragments arranged by size:
3676 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6154
AvaI C'yCGr_G
Cuts at: 1870 1943 2055 2110 2121 3479 1870
Size: 73 112 55 11 1358 4545
Fragments arranged by size:
4545 1358 112 73 55 11
BamHI G'GATC_C
Cuts at: 3484 3851 3484
Size: 367 5787
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3475 4930 618
Size: 325 217 627 1688 1455 1842
Fragments arranged by size:
1842 1688 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2091 2228 3278 3326 3350 3473 1391
Size: 700 137 1050 48 24 123 4072
Fragments arranged by size:
4072 1050 700 137 123 48 24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2399 3251 928
Size: 971 500 852 3831
Fragments arranged by size:
3831 971 852 500
BbvCI CC'TCA_GC
Cuts at: 2787 2832 3308 2787
Size: 45 476 5633
Fragments arranged by size:
5633 476 45
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2795 2829 4345 4379 1903
Size: 34 858 34 1516 34 3678
Fragments arranged by size:
3678 1516 858 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4046 5256 4046
Size: 1210 4944
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6154
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2157 2979 4765
Size: 107 122 71 631 605 485 822 1786
Cuts at: 4765 136
Size: 1525
Fragments arranged by size:
1786 1525 822 631 605 485 122 107
71
BglII A'GATC_T
Cuts at: 2718 2922 6149 2718
Size: 204 3227 2723
Fragments arranged by size:
3227 2723 204
BlpI GC'TnA_GC
Cuts at: 2222 3571 2222
Size: 1349 4805
Bme1580I G_kGCm'C
Cuts at: 1790 2091 4215 5371 1790
Size: 301 2124 1156 2573
Fragments arranged by size:
2573 2124 1156 301
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6154
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3885 4843 442
Size: 416 53 83 399 24 2468 958 1753
Fragments arranged by size:
2468 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2258 2360 2381 2477 2507 2654 2939 3017
Size: 1019 102 21 96 30 147 285 78
Cuts at: 3017 3092 3209 3230 3310 3334 3361 4814 1239
Size: 75 117 21 80 24 27 1453 2579
Fragments arranged by size:
2579 1453 1019 285 147 117 102 96
80 78 75 30 27 24 21 21
Bpu10I CC'TnA_GC
Cuts at: 2787 2832 3308 2787
Size: 45 476 5633
Fragments arranged by size:
5633 476 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4279 5144 5442 4279
Size: 865 298 4991
Fragments arranged by size:
4991 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2357 4817 882
Size: 617 858 2460 2219
Fragments arranged by size:
2460 2219 858 617
BsaAI yAC'GTr
Cuts at: 493 1204 3410 3945 493
Size: 711 2206 535 2702
Fragments arranged by size:
2702 2206 711 535
BsaBI GATnn'nnATC
Cuts at: 6148 6148
Size: 6154
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3441 3961 4343 4978
Size: 53 83 186 936 1908 520 382 635
Cuts at: 4978 275
Size: 1451
Fragments arranged by size:
1908 1451 936 635 520 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2937 4587 4985 5259 5406 5817 2937
Size: 1650 398 274 147 411 3274
Fragments arranged by size:
3274 1650 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2354 2384 2696 2726 2744
Size: 30 165 30 951 30 312 30 18
Cuts at: 2744 2774 1178
Size: 30 4558
Fragments arranged by size:
4558 951 312 165 30 30 30 30
30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2098 2708 2879 3212 3364 1186
Size: 912 610 171 333 152 3976
Fragments arranged by size:
3976 912 610 333 171 152
BseYI C'CCAG_C
Cuts at: 2882 5357 5853 2882
Size: 2475 496 3183
Fragments arranged by size:
3183 2475 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5581
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3604 4365 5393 1540
Size: 275 186 1603 761 1028 2301
Fragments arranged by size:
2301 1603 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3278 4215 4300 5371 3278
Size: 937 85 1071 4061
Fragments arranged by size:
4061 1071 937 85
BsmI GAATG_Cn'
Cuts at: 3665 3758 3665
Size: 93 6061
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2228 3278 3326 3350 3473 4215
Size: 399 301 137 1050 48 24 123 742
Cuts at: 4215 4300 5371 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1050 742 399 301 137 123
85 48 24
BspEI T'CCGG_A
Cuts at: 5817 5817
Size: 6154
BspHI T'CATG_A
Cuts at: 4043 5773 4043
Size: 1730 4424
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2910 3254 3509 844
Size: 2066 344 255 3489
Fragments arranged by size:
3489 2066 344 255
BsrBI CCG'CTC
Cuts at: 1394 1823 2073 2760 4041 1394
Size: 429 250 687 1281 3507
Fragments arranged by size:
3507 1281 687 429 250
BsrDI GCAATG_nn'
Cuts at: 65 1248 2781 3006 4649 4823 65
Size: 1183 1533 225 1643 174 1396
Fragments arranged by size:
1643 1533 1396 1183 225 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2949 3204 4798 4985 1700
Size: 110 1139 255 1594 187 2869
Fragments arranged by size:
2869 1594 1139 255 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6154
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6154
BssSI C'ACGA_G
Cuts at: 2080 4214 5226 2080
Size: 2134 1012 3008
Fragments arranged by size:
3008 2134 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6154
BstBI TT'CG_AA
Cuts at: 4958 4958
Size: 6154
BstEII G'GTnAC_C
Cuts at: 1184 2017 1184
Size: 833 5321
BstXI CCAn_nnnn'nTGG
Cuts at: 2182 3385 2182
Size: 1203 4951
BstYI r'GATC_y
Cuts at: 1972 2697 2718 2922 3484 3851 4236 4253 5705
Size: 725 21 204 562 367 385 17 1452
Cuts at: 5705 5791 5803 6149 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 725 562 385 367 346 204
86 21 17 12
Bsu36I CC'TnA_GG
Cuts at: 2444 2675 2444
Size: 231 5923
BtgI C'CryG_G
Cuts at: 513 1065 2745 3384 513
Size: 552 1680 639 3283
Fragments arranged by size:
3283 1680 639 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5415
Fragments arranged by size:
5415 683 56
BtsI GCAGTG_nn'
Cuts at: 3670 4464 4484 3670
Size: 794 20 5340
Fragments arranged by size:
5340 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6154
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6119
DraI TTT'AAA
Cuts at: 3458 3458
Size: 6154
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2026 5161 809
Size: 507 710 3135 1802
Fragments arranged by size:
3135 1802 710 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3036 3601 4490
Size: 54 1425 50 275 18 1206 565 889
Cuts at: 4490 6119 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1206 889 565 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3601 1537
Size: 275 1789 4090
Fragments arranged by size:
4090 1789 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2685 4084 1353
Size: 1332 1399 3423
Fragments arranged by size:
3423 1399 1332
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2507 4741 5115 5261 387
Size: 1064 138 918 2234 374 146 1280
Fragments arranged by size:
2234 1280 1064 918 374 146 138
EcoICRI GAG'CTC
Cuts at: 3276 3276
Size: 6154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2047 2258 2360 2381 2477 2501 2507
Size: 393 415 211 102 21 96 24 6
Cuts at: 2507 2654 2939 3017 3092 3209 3230 3310 3334
Size: 147 285 78 75 117 21 80 24
Cuts at: 3334 3343 3361 4217 4814 5601 5869 1239
Size: 9 18 856 597 787 268 1524
Fragments arranged by size:
1524 856 787 597 415 393 285 268
211 147 117 102 96 80 78 75
24 24 21 21 18 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3449 3449
Size: 6154
EcoO109I rG'GnC_Cy
Cuts at: 2971 3289 2971
Size: 318 5836
EcoRI G'AATT_C
Cuts at: 3463 3841 3463
Size: 378 5776
FspI TGC'GCA
Cuts at: 2267 4660 2267
Size: 2393 3761
FspAI rTGC'GCAy
Cuts at: 2267 2267
Size: 6154
HaeII r_GCGC'y
Cuts at: 1678 5301 1678
Size: 3623 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2621 2653 2696 2728 4795
Size: 32 135 32 1049 32 43 32 2067
Cuts at: 4795 4827 4869 4901 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2067 1049 135 43 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2209 3498 3744 669
Size: 988 281 271 1289 246 3079
Fragments arranged by size:
3079 1289 988 281 271 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6154
HpaI GTT'AAC
Cuts at: 3744 3744
Size: 6154
KpnI G_GTAC'C
Cuts at: 3479 3479
Size: 6154
MfeI C'AATT_G
Cuts at: 3753 3753
Size: 6154
MluI A'CGCG_T
Cuts at: 6030 6030
Size: 6154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5268 5452 1305
Size: 3963 184 2007
Fragments arranged by size:
3963 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3038 10
Size: 54 1425 343 1206 3126
Fragments arranged by size:
3126 1425 1206 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2015 2732 2895 4112 4471 4630 518
Size: 648 849 717 163 1217 359 159 2042
Fragments arranged by size:
2042 1217 849 717 648 359 163 159
MspA1I CmG'CkG
Cuts at: 1606 2178 2455 3088 3115 3255 3570 4247 5395
Size: 572 277 633 27 140 315 677 1148
Cuts at: 5395 5640 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 633 572 315 277 245
140 27
NaeI GCC'GGC
Cuts at: 2951 3206 2951
Size: 255 5899
NcoI C'CATG_G
Cuts at: 513 1065 2745 513
Size: 552 1680 3922
Fragments arranged by size:
3922 1680 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6154
NgoMIV G'CCGG_C
Cuts at: 2949 3204 2949
Size: 255 5899
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6154
NotI GC'GGCC_GC
Cuts at: 3601 3601
Size: 6154
NspI r_CATG'y
Cuts at: 1132 1229 2422 2509 2848 3512 5057 1132
Size: 97 1193 87 339 664 1545 2229
Fragments arranged by size:
2229 1545 1193 664 339 97 87
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6154
PciI A'CATG_T
Cuts at: 2418 5053 2418
Size: 2635 3519
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6154
PfoI T'CCnGG_A
Cuts at: 2235 2337 2700 2235
Size: 102 363 5689
Fragments arranged by size:
5689 363 102
PmeI GTTT'AAAC
Cuts at: 3458 3458
Size: 6154
PmlI CAC'GTG
Cuts at: 3410 3410
Size: 6154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4223 4255 5736 5768 4223
Size: 32 1481 32 4609
Fragments arranged by size:
4609 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6154
PsiI TTA'TAA
Cuts at: 3724 3724
Size: 6154
PspOMI G'GGCC_C
Cuts at: 2087 2087
Size: 6154
PspXI vC'TCGA_Gb
Cuts at: 2110 2110
Size: 6154
PstI C_TGCA'G
Cuts at: 830 2385 2712 2874 3087 3506 830
Size: 1555 327 162 213 419 3478
Fragments arranged by size:
3478 1555 419 327 213 162
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6154
PvuII CAG'CTG
Cuts at: 2455 3115 2455
Size: 660 5494
SacI G_AGCT'C
Cuts at: 3278 3278
Size: 6154
SalI G'TCGA_C
Cuts at: 1936 3496 1936
Size: 1560 4594
SbfI CC_TGCA'GG
Cuts at: 3506 3506
Size: 6154
ScaI AGT'ACT
Cuts at: 4402 6114 4402
Size: 1712 4442
SfcI C'TryA_G
Cuts at: 826 1045 2381 2708 2870 3083 3175 3313 3502
Size: 219 1336 327 162 213 92 138 189
Cuts at: 3502 4637 5318 5509 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1336 1135 681 327 219 213 191
189 162 138 92
SfiI GGCCn_nnn'nGGCC
Cuts at: 2979 2979
Size: 6154
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6154
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6154
SmaI CCC'GGG
Cuts at: 1872 2057 3481 1872
Size: 185 1424 4545
Fragments arranged by size:
4545 1424 185
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2110 4258 5159 5421 820
Size: 197 926 167 2148 901 262 1553
Fragments arranged by size:
2148 1553 926 901 262 197 167
SnaBI TAC'GTA
Cuts at: 493 3945 493
Size: 3452 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6154
SphI G_CATG'C
Cuts at: 1132 3512 1132
Size: 2380 3774
SspI AAT'ATT
Cuts at: 5 52 4078 5
Size: 47 4026 2081
Fragments arranged by size:
4026 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6154
StyI C'CwwG_G
Cuts at: 513 1065 2745 3450 3593 513
Size: 552 1680 705 143 3074
Fragments arranged by size:
3074 1680 705 552 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2285 2672 4181 4198 4351 4536 4981
Size: 1255 705 387 1509 17 153 185 445
Cuts at: 4981 325
Size: 1498
Fragments arranged by size:
1509 1498 1255 705 445 387 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4400 6112 96
Size: 274 80 33 51 1443 2423 1712 138
Fragments arranged by size:
2423 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2354 2386 6114 6146 2354
Size: 32 3728 32 2362
Fragments arranged by size:
3728 2362 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6154
XbaI T'CTAG_A
Cuts at: 3490 3490
Size: 6154
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3100 3100
Size: 6154
XhoI C'TCGA_G
Cuts at: 1943 2110 1943
Size: 167 5987
XmaI C'CCGG_G
Cuts at: 1870 2055 3479 1870
Size: 185 1424 4545
Fragments arranged by size:
4545 1424 185
XmnI GAAnn'nnTTC
Cuts at: 4283 4283
Size: 6154
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3442 3962 276
Size: 53 83 186 936 1908 520 2468
Fragments arranged by size:
2468 1908 936 520 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoRV FalI FseI KasI NarI
NruI NsiI PacI PpuMI PsrI RsrII SacII SanDI
SapI SexAI SfoI SrfI SwaI