Restriction map for
pFN21AB8357
(Circular) (Six-base) MAPSORT of: pFN21AB8357.seq Check: 9939 from: 1 to: 5494
pFN21AB8357 5494 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2052 2195 3304 278
Size: 53 83 186 936 516 143 1109 2468
Fragments arranged by size:
2468 1109 936 516 186 143 83 53
AccI GT'mk_AC
Cuts at: 1937 2058 2837 1937
Size: 121 779 4594
Fragments arranged by size:
4594 779 121
Acc65I G'GTAC_C
Cuts at: 2815 2815
Size: 5494
AclI AA'CG_TT
Cuts at: 1563 3621 3994 1563
Size: 2058 373 3063
Fragments arranged by size:
3063 2058 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3557 4941 5209 1632
Size: 1925 1384 268 1917
Fragments arranged by size:
1925 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5297
AflIII A'CryG_T
Cuts at: 1203 4393 5370 1203
Size: 3190 977 1327
Fragments arranged by size:
3190 1327 977
AgeI A'CCGG_T
Cuts at: 4325 4325
Size: 5494
AhdI GACnn_n'nnGTC
Cuts at: 2191 4223 2191
Size: 2032 3462
AleI CACnn'nnGTG
Cuts at: 1166 2243 1166
Size: 1077 4417
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5494
AlwNI CAG_nnn'CTG
Cuts at: 3237 4809 3237
Size: 1572 3922
ApaLI G'TGCA_C
Cuts at: 3551 4707 3551
Size: 1156 4338
ApoI r'AATT_y
Cuts at: 1493 2405 2803 3029 3181 5290 1493
Size: 912 398 226 152 2109 1697
Fragments arranged by size:
2109 1697 912 398 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4048 160
Size: 872 3016 1606
Fragments arranged by size:
3016 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5494
AvaI C'yCGr_G
Cuts at: 1870 1943 2529 2819 1870
Size: 73 586 290 4545
Fragments arranged by size:
4545 586 290 73
BamHI G'GATC_C
Cuts at: 2636 2824 3191 2636
Size: 188 367 4939
Fragments arranged by size:
4939 367 188
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2815 4270 618
Size: 325 217 627 1028 1455 1842
Fragments arranged by size:
1842 1455 1028 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2205 2813 1391
Size: 814 608 4072
Fragments arranged by size:
4072 814 608
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4523
BbvCI CC'TCA_GC
Cuts at: 2141 2141
Size: 5494
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2257 2291 3685 3719 1903
Size: 34 320 34 1394 34 3678
Fragments arranged by size:
3678 1394 320 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3386 4596 3386
Size: 1210 4284
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5494
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4105 136
Size: 107 122 71 631 605 2433 1525
Fragments arranged by size:
2433 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5489 5489
Size: 5494
BlpI GC'TnA_GC
Cuts at: 2558 2911 2558
Size: 353 5141
Bme1580I G_kGCm'C
Cuts at: 1790 3555 4711 1790
Size: 1765 1156 2573
Fragments arranged by size:
2573 1765 1156
BmgBI CAC'GTC
Cuts at: 1318 2056 1318
Size: 738 4756
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2658 3225 4183
Size: 416 53 83 399 24 1241 567 958
Cuts at: 4183 442
Size: 1753
Fragments arranged by size:
1753 1241 958 567 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5494
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2201 4154 1239
Size: 962 1953 2579
Fragments arranged by size:
2579 1953 962
Bpu10I CC'TnA_GC
Cuts at: 2141 2211 2141
Size: 70 5424
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3619 4484 4782 3619
Size: 865 298 4331
Fragments arranged by size:
4331 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4157 882
Size: 617 2658 2219
Fragments arranged by size:
2658 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3285 493
Size: 711 2081 2702
Fragments arranged by size:
2702 2081 711
BsaBI GATnn'nnATC
Cuts at: 2285 5488 2285
Size: 3203 2291
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2049 2192 3301 3683
Size: 53 83 186 936 516 143 1109 382
Cuts at: 3683 4318 275
Size: 635 1451
Fragments arranged by size:
1451 1109 936 635 516 382 186 143
83 53
BsaWI w'CCGG_w
Cuts at: 3927 4325 4599 4746 5157 3927
Size: 398 274 147 411 4264
Fragments arranged by size:
4264 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2003 2033 1178
Size: 30 165 30 600 30 4639
Fragments arranged by size:
4639 600 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 1186
Size: 838 4656
BseYI C'CCAG_C
Cuts at: 2519 2766 2781 4697 5193 2519
Size: 247 15 1916 496 2820
Fragments arranged by size:
2820 1916 496 247 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4921
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2944 3705 4733 1540
Size: 275 186 943 761 1028 2301
Fragments arranged by size:
2301 1028 943 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2034 2205 2680 3555 3640 4711 2034
Size: 171 475 875 85 1071 2817
Fragments arranged by size:
2817 1071 875 475 171 85
BsmI GAATG_Cn'
Cuts at: 3005 3098 3005
Size: 93 5401
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2205 2680 2813 3555 3640 4711
Size: 399 244 171 475 133 742 85 1071
Cuts at: 4711 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 475 399 244 171 133
85
BspEI T'CCGG_A
Cuts at: 5157 5157
Size: 5494
BspHI T'CATG_A
Cuts at: 3383 5113 3383
Size: 1730 3764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2259 2849 844
Size: 1415 590 3489
Fragments arranged by size:
3489 1415 590
BsrBI CCG'CTC
Cuts at: 1394 1823 2016 3381 1394
Size: 429 193 1365 3507
Fragments arranged by size:
3507 1365 429 193
BsrDI GCAATG_nn'
Cuts at: 65 1248 2366 3989 4163 65
Size: 1183 1118 1623 174 1396
Fragments arranged by size:
1623 1396 1183 1118 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2136 4138 4325 1700
Size: 110 326 2002 187 2869
Fragments arranged by size:
2869 2002 326 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5494
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5494
BssSI C'ACGA_G
Cuts at: 3554 4566 3554
Size: 1012 4482
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5494
BstBI TT'CG_AA
Cuts at: 4298 4298
Size: 5494
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5494
BstYI r'GATC_y
Cuts at: 1972 2636 2824 3191 3576 3593 5045 5131 5143
Size: 664 188 367 385 17 1452 86 12
Cuts at: 5143 5489 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 664 385 367 346 188 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2072 2086 513
Size: 552 939 68 14 3921
Fragments arranged by size:
3921 939 552 68 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2473 487
Size: 683 56 1247 3508
Fragments arranged by size:
3508 1247 683 56
BtsI GCAGTG_nn'
Cuts at: 2148 2405 2709 3010 3804 3824 2148
Size: 257 304 301 794 20 3818
Fragments arranged by size:
3818 794 304 301 257 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5494
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5459
DraI TTT'AAA
Cuts at: 2798 2798
Size: 5494
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2054 4501 809
Size: 507 738 2447 1802
Fragments arranged by size:
2447 1802 738 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2134 2690 2941
Size: 54 1425 50 275 18 304 556 251
Cuts at: 2941 3830 5459 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 556 304 275 251 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2941 1537
Size: 275 1129 4090
Fragments arranged by size:
4090 1129 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3424 1353
Size: 2071 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4081 4455 4601 387
Size: 1064 138 2492 374 146 1280
Fragments arranged by size:
2492 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2203 2203
Size: 5494
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 3557 4154 4941 5209 1239
Size: 393 569 1356 597 787 268 1524
Fragments arranged by size:
1524 1356 787 597 569 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2577 2577
Size: 5494
EcoO109I rG'GnC_Cy
Cuts at: 2215 2438 2215
Size: 223 5271
EcoRI G'AATT_C
Cuts at: 2803 3181 2803
Size: 378 5116
FspI TGC'GCA
Cuts at: 4000 4000
Size: 5494
HaeII r_GCGC'y
Cuts at: 1678 4641 1678
Size: 2963 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2108 2140 2177 2209 2628
Size: 32 135 32 536 32 37 32 419
Cuts at: 2628 2660 4135 4167 4209 4241 1373
Size: 32 1475 32 42 32 2626
Fragments arranged by size:
2626 1475 536 419 135 42 37 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2838 3084 669
Size: 988 281 900 246 3079
Fragments arranged by size:
3079 988 900 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5494
HpaI GTT'AAC
Cuts at: 3084 3084
Size: 5494
KpnI G_GTAC'C
Cuts at: 2819 2819
Size: 5494
MfeI C'AATT_G
Cuts at: 3093 3093
Size: 5494
MluI A'CGCG_T
Cuts at: 5370 5370
Size: 5494
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4608 4792 1305
Size: 3303 184 2007
Fragments arranged by size:
3303 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2692 10
Size: 54 1425 343 860 2812
Fragments arranged by size:
2812 1425 860 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2243 3452 3811 3970 518
Size: 648 1077 1209 359 159 2042
Fragments arranged by size:
2042 1209 1077 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2074 2113 2243 2519 2910 3587 4735 4980
Size: 468 39 130 276 391 677 1148 245
Cuts at: 4980 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 468 391 276 245 130
39
NaeI GCC'GGC
Cuts at: 2138 2138
Size: 5494
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4003
Fragments arranged by size:
4003 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5494
NgoMIV G'CCGG_C
Cuts at: 2136 2136
Size: 5494
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5494
NotI GC'GGCC_GC
Cuts at: 2941 2941
Size: 5494
NspI r_CATG'y
Cuts at: 1132 1229 2852 4397 1132
Size: 97 1623 1545 2229
Fragments arranged by size:
2229 1623 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2579 2612 1348
Size: 1231 33 4230
Fragments arranged by size:
4230 1231 33
PciI A'CATG_T
Cuts at: 4393 4393
Size: 5494
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5494
PmeI GTTT'AAAC
Cuts at: 2798 2798
Size: 5494
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3563 3595 5076 5108 3563
Size: 32 1481 32 3949
Fragments arranged by size:
3949 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5494
PsiI TTA'TAA
Cuts at: 3064 3064
Size: 5494
PstI C_TGCA'G
Cuts at: 830 2846 830
Size: 2016 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5494
PvuII CAG'CTG
Cuts at: 2113 2243 2519 2113
Size: 130 276 5088
Fragments arranged by size:
5088 276 130
SacI G_AGCT'C
Cuts at: 2205 2205
Size: 5494
SacII CC_GC'GG
Cuts at: 2075 2075
Size: 5494
SalI G'TCGA_C
Cuts at: 1936 2836 1936
Size: 900 4594
SbfI CC_TGCA'GG
Cuts at: 2846 2846
Size: 5494
ScaI AGT'ACT
Cuts at: 3742 5454 3742
Size: 1712 3782
SfcI C'TryA_G
Cuts at: 826 1045 2092 2742 2842 3977 4658 4849 826
Size: 219 1047 650 100 1135 681 191 1471
Fragments arranged by size:
1471 1135 1047 681 650 219 191 100
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5494
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5494
SmaI CCC'GGG
Cuts at: 1872 2821 1872
Size: 949 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3598 4499 4761 820
Size: 197 926 1655 901 262 1553
Fragments arranged by size:
1655 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3285 493
Size: 2792 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5494
SphI G_CATG'C
Cuts at: 1132 2852 1132
Size: 1720 3774
SspI AAT'ATT
Cuts at: 5 52 3418 5
Size: 47 3366 2081
Fragments arranged by size:
3366 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5494
StyI C'CwwG_G
Cuts at: 513 1065 2004 2434 2933 513
Size: 552 939 430 499 3074
Fragments arranged by size:
3074 939 552 499 430
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2352 2377 3521 3538 3691 3876 4321
Size: 1255 772 25 1144 17 153 185 445
Cuts at: 4321 325
Size: 1498
Fragments arranged by size:
1498 1255 1144 772 445 185 153 25
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3740 5452 96
Size: 274 80 33 51 1443 1763 1712 138
Fragments arranged by size:
1763 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5454 5486 5454
Size: 32 5462
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5494
XbaI T'CTAG_A
Cuts at: 2830 2830
Size: 5494
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2778 2778
Size: 5494
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5494
XmaI C'CCGG_G
Cuts at: 1870 2819 1870
Size: 949 4545
XmnI GAAnn'nnTTC
Cuts at: 3623 3623
Size: 5494
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2050 2193 3302 276
Size: 53 83 186 936 516 143 1109 2468
Fragments arranged by size:
2468 1109 936 516 186 143 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SanDI SapI SexAI SfiI SfoI SrfI SwaI