Restriction map for
pFN21AB8355
(Circular) (Six-base) MAPSORT of: pFN21AB8355.seq Check: 8874 from: 1 to: 6658
pFN21AB8355 6658 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3753 3753
Size: 6658
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4468 278
Size: 53 83 186 936 2932 2468
Fragments arranged by size:
2932 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2346 4001 1937
Size: 409 1655 4594
Fragments arranged by size:
4594 1655 409
Acc65I G'GTAC_C
Cuts at: 3979 3979
Size: 6658
AclI AA'CG_TT
Cuts at: 1563 4785 5158 1563
Size: 3222 373 3063
Fragments arranged by size:
3222 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3787 4721 6105 6373 1632
Size: 2155 934 1384 268 1917
Fragments arranged by size:
2155 1917 1384 934 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6461
AflIII A'CryG_T
Cuts at: 1203 5557 6534 1203
Size: 4354 977 1327
Fragments arranged by size:
4354 1327 977
AgeI A'CCGG_T
Cuts at: 5489 5489
Size: 6658
AhdI GACnn_n'nnGTC
Cuts at: 5387 5387
Size: 6658
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6658
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2046 1184
Size: 862 5796
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3260 3292 3260
Size: 32 6626
AlwNI CAG_nnn'CTG
Cuts at: 4401 5973 4401
Size: 1572 5086
ApaI G_GGCC'C
Cuts at: 3076 3496 3076
Size: 420 6238
ApaLI G'TGCA_C
Cuts at: 4715 5871 4715
Size: 1156 5502
ApoI r'AATT_y
Cuts at: 1493 3967 4193 4345 6454 1493
Size: 2474 226 152 2109 1697
Fragments arranged by size:
2474 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5212 160
Size: 872 4180 1606
Fragments arranged by size:
4180 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6658
AvaI C'yCGr_G
Cuts at: 1870 1943 3243 3508 3983 1870
Size: 73 1300 265 475 4545
Fragments arranged by size:
4545 1300 475 265 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3504 3537 3790 3823 3504
Size: 33 253 33 6339
Fragments arranged by size:
6339 253 33 33
BamHI G'GATC_C
Cuts at: 3988 4355 3988
Size: 367 6291
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2390 2489 2995 3235 3535
Size: 325 217 627 603 99 506 240 300
Cuts at: 3535 3979 5434 618
Size: 444 1455 1842
Fragments arranged by size:
1842 1455 627 603 506 444 325 300
240 217 99
BanII G_rGCy'C
Cuts at: 1391 2101 2484 3023 3076 3150 3222 3489 3496
Size: 710 383 539 53 74 72 267 7
Cuts at: 3496 3669 3741 3846 3977 1391
Size: 173 72 105 131 4072
Fragments arranged by size:
4072 710 539 383 267 173 131 105
74 72 72 53 7
BbeI G_GCGC'C
Cuts at: 2999 3239 2999
Size: 240 6418
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2382 2510 928
Size: 971 483 128 5076
Fragments arranged by size:
5076 971 483 128
BbvCI CC'TCA_GC
Cuts at: 3700 3700
Size: 6658
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3572 3606 4849 4883 1903
Size: 34 1635 34 1243 34 3678
Fragments arranged by size:
3678 1635 1243 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4550 5760 4550
Size: 1210 5448
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6658
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3194 5269 136
Size: 107 122 71 631 605 1522 2075 1525
Fragments arranged by size:
2075 1525 1522 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6653 6653
Size: 6658
BlpI GC'TnA_GC
Cuts at: 3057 4075 3057
Size: 1018 5640
Bme1580I G_kGCm'C
Cuts at: 1790 2492 3076 3496 3540 4719 5875 1790
Size: 702 584 420 44 1179 1156 2573
Fragments arranged by size:
2573 1179 1156 702 584 420 44
BmgBI CAC'GTC
Cuts at: 1318 2350 1318
Size: 1032 5626
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2272 3914 4389
Size: 416 53 83 399 24 855 1642 475
Cuts at: 4389 5347 442
Size: 958 1753
Fragments arranged by size:
1753 1642 958 855 475 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6658
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2097 3106 2097
Size: 1009 5649
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2077 2416 3016 3461 3737 3870 5318 1239
Size: 838 339 600 445 276 133 1448 2579
Fragments arranged by size:
2579 1448 838 600 445 339 276 133
Bpu10I CC'TnA_GC
Cuts at: 2319 2621 3621 3700 2319
Size: 302 1000 79 5277
Fragments arranged by size:
5277 1000 302 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2117 3728 4783 5648 5946 2117
Size: 1611 1055 865 298 2829
Fragments arranged by size:
2829 1611 1055 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2801 3131 3167 3755 5321 882
Size: 617 1302 330 36 588 1566 2219
Fragments arranged by size:
2219 1566 1302 617 588 330 36
BsaAI yAC'GTr
Cuts at: 493 1204 2251 2314 4449 493
Size: 711 1047 63 2135 2702
Fragments arranged by size:
2702 2135 1047 711 63
BsaBI GATnn'nnATC
Cuts at: 3896 6652 3896
Size: 2756 3902
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2996 3236 3336 4465
Size: 53 83 186 936 1463 240 100 1129
Cuts at: 4465 4847 5482 275
Size: 382 635 1451
Fragments arranged by size:
1463 1451 1129 936 635 382 240 186
100 83 53
BsaWI w'CCGG_w
Cuts at: 2985 3559 5091 5489 5763 5910 6321 2985
Size: 574 1532 398 274 147 411 3322
Fragments arranged by size:
3322 1532 574 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3809 3839 1178
Size: 30 165 30 2406 30 3997
Fragments arranged by size:
3997 2406 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2603 2606 2681 2723 2813 2855 3082 3185
Size: 1417 3 75 42 90 42 227 103
Cuts at: 3185 3230 3725 3833 3836 3947 3950 1186
Size: 45 495 108 3 111 3 3894
Fragments arranged by size:
3894 1417 495 227 111 108 103 90
75 45 42 42 3 3 3
BseYI C'CCAG_C
Cuts at: 2009 2862 3149 3906 5861 6357 2009
Size: 853 287 757 1955 496 2310
Fragments arranged by size:
2310 1955 853 757 496 287
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2429 1163
Size: 573 693 5392
Fragments arranged by size:
5392 693 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4108 4869 5897 1540
Size: 275 186 2107 761 1028 2301
Fragments arranged by size:
2301 2107 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2256 2715 3846 4719 4804 5875 2256
Size: 459 1131 873 85 1071 3039
Fragments arranged by size:
3039 1131 1071 873 459 85
BsmI GAATG_Cn'
Cuts at: 4169 4262 4169
Size: 93 6565
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2101 2256 2484 2492 2715 3023 3076
Size: 399 311 155 228 8 223 308 53
Cuts at: 3076 3150 3222 3489 3496 3540 3669 3741 3846
Size: 74 72 267 7 44 129 72 105
Cuts at: 3846 3977 4719 4804 5875 1391
Size: 131 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 311 308 267 228
223 155 131 129 105 85 74 72
72 53 44 8 7
BspEI T'CCGG_A
Cuts at: 3559 6321 3559
Size: 2762 3896
BspHI T'CATG_A
Cuts at: 4547 6277 4547
Size: 1730 4928
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3753 4013 844
Size: 2909 260 3489
Fragments arranged by size:
3489 2909 260
BsrBI CCG'CTC
Cuts at: 1394 1823 2596 2638 2653 2677 2692 3070 4545
Size: 429 773 42 15 24 15 378 1475
Cuts at: 4545 1394
Size: 3507
Fragments arranged by size:
3507 1475 773 429 378 42 24 15
15
BsrDI GCAATG_nn'
Cuts at: 65 1248 5153 5327 65
Size: 1183 3905 174 1396
Fragments arranged by size:
3905 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3203 5302 5489 1700
Size: 110 1393 2099 187 2869
Fragments arranged by size:
2869 2099 1393 187 110
BsrGI T'GTAC_A
Cuts at: 96 2103 96
Size: 2007 4651
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6658
BssSI C'ACGA_G
Cuts at: 2084 4718 5730 2084
Size: 2634 1012 3012
Fragments arranged by size:
3012 2634 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2452 794
Size: 1658 5000
BstBI TT'CG_AA
Cuts at: 5462 5462
Size: 6658
BstEII G'GTnAC_C
Cuts at: 1184 2783 3085 3520 1184
Size: 1599 302 435 4322
Fragments arranged by size:
4322 1599 435 302
BstXI CCAn_nnnn'nTGG
Cuts at: 2314 2371 2314
Size: 57 6601
BstYI r'GATC_y
Cuts at: 1972 3988 4355 4740 4757 6209 6295 6307 6653
Size: 2016 367 385 17 1452 86 12 346
Cuts at: 6653 1972
Size: 1977
Fragments arranged by size:
2016 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3192 3225 3192
Size: 33 6625
BtgI C'CryG_G
Cuts at: 513 1065 2004 2018 2484 3210 3315 3339 513
Size: 552 939 14 466 726 105 24 3832
Fragments arranged by size:
3832 939 726 552 466 105 24 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5919
Fragments arranged by size:
5919 683 56
BtsI GCAGTG_nn'
Cuts at: 2221 3107 3378 3384 3566 4174 4968 4988 2221
Size: 886 271 6 182 608 794 20 3891
Fragments arranged by size:
3891 886 794 608 271 182 20 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6658
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6623
DraI TTT'AAA
Cuts at: 3962 3962
Size: 6658
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5665 809
Size: 507 4349 1802
Fragments arranged by size:
4349 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 4105 4994
Size: 54 1425 50 275 18 210 2065 889
Cuts at: 4994 6623 8
Size: 1629 43
Fragments arranged by size:
2065 1629 1425 889 275 210 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4105 1537
Size: 275 2293 4090
Fragments arranged by size:
4090 2293 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2568 2739 2799 3834 3924 4588 1353
Size: 1215 171 60 1035 90 664 3423
Fragments arranged by size:
3423 1215 1035 664 171 90 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3849 5245 5619 5765 387
Size: 1064 138 2260 1396 374 146 1280
Fragments arranged by size:
2260 1396 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3844 3844
Size: 6658
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2077 2416 3016 3461 3737 3787 3870
Size: 393 445 339 600 445 276 50 83
Cuts at: 3870 4721 5318 6105 6373 1239
Size: 851 597 787 268 1524
Fragments arranged by size:
1524 851 787 600 597 445 445 393
339 276 268 83 50
EcoNI CCTnn'n_nnAGG
Cuts at: 3080 3530 3080
Size: 450 6208
EcoO109I rG'GnC_Cy
Cuts at: 2605 3073 3465 3492 3493 3780 2605
Size: 468 392 27 1 287 5483
Fragments arranged by size:
5483 468 392 287 27 1
EcoRI G'AATT_C
Cuts at: 3967 4345 3967
Size: 378 6280
FseI GG_CCGG'CC
Cuts at: 3207 3207
Size: 6658
FspI TGC'GCA
Cuts at: 5164 5164
Size: 6658
HaeII r_GCGC'y
Cuts at: 1678 2683 2999 3239 5805 1678
Size: 1005 316 240 2566 2531
Fragments arranged by size:
2566 2531 1005 316 240
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2067 2099 2927 2959 3676
Size: 32 135 32 495 32 828 32 717
Cuts at: 3676 3708 5299 5331 5373 5405 1373
Size: 32 1591 32 42 32 2626
Fragments arranged by size:
2626 1591 828 717 495 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2347 4002 4248 669
Size: 988 281 409 1655 246 3079
Fragments arranged by size:
3079 1655 988 409 281 246
HindIII A'AGCT_T
Cuts at: 748 2135 748
Size: 1387 5271
HpaI GTT'AAC
Cuts at: 4248 4248
Size: 6658
KasI G'GCGC_C
Cuts at: 2995 3235 2995
Size: 240 6418
KpnI G_GTAC'C
Cuts at: 3983 3983
Size: 6658
MfeI C'AATT_G
Cuts at: 4257 4257
Size: 6658
MluI A'CGCG_T
Cuts at: 6534 6534
Size: 6658
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2811 5772 5956 1305
Size: 1506 2961 184 2007
Fragments arranged by size:
2961 2007 1506 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2042 10
Size: 54 1425 343 210 4626
Fragments arranged by size:
4626 1425 343 210 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2793 4616 4975 5134 518
Size: 648 1627 1823 359 159 2042
Fragments arranged by size:
2042 1823 1627 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2404 2516 2866 2931 3332 3359 3422 4074
Size: 798 112 350 65 401 27 63 652
Cuts at: 4074 4751 5899 6144 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 798 677 652 401 350 245
112 65 63 27
NaeI GCC'GGC
Cuts at: 3205 3205
Size: 6658
NarI GG'CG_CC
Cuts at: 2996 3236 2996
Size: 240 6418
NcoI C'CATG_G
Cuts at: 513 1065 2004 2018 3210 513
Size: 552 939 14 1192 3961
Fragments arranged by size:
3961 1192 939 552 14
NdeI CA'TA_TG
Cuts at: 387 2110 387
Size: 1723 4935
NgoMIV G'CCGG_C
Cuts at: 3203 3203
Size: 6658
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6658
NotI GC'GGCC_GC
Cuts at: 4105 4105
Size: 6658
NspI r_CATG'y
Cuts at: 1132 1229 3906 4016 5561 1132
Size: 97 2677 110 1545 2229
Fragments arranged by size:
2677 2229 1545 110 97
PasI CC'CwG_GG
Cuts at: 1348 3489 1348
Size: 2141 4517
PciI A'CATG_T
Cuts at: 5557 5557
Size: 6658
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2024 1108
Size: 916 5742
PmeI GTTT'AAAC
Cuts at: 3962 3962
Size: 6658
PmlI CAC'GTG
Cuts at: 2314 2314
Size: 6658
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4727 4759 6240 6272 4727
Size: 32 1481 32 5113
Fragments arranged by size:
5113 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3780 3780
Size: 6658
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6658
PsiI TTA'TAA
Cuts at: 4228 4228
Size: 6658
PspOMI G'GGCC_C
Cuts at: 3072 3492 3072
Size: 420 6238
PstI C_TGCA'G
Cuts at: 830 2035 2096 2216 3265 3292 4010 830
Size: 1205 61 120 1049 27 718 3478
Fragments arranged by size:
3478 1205 1049 718 120 61 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6658
PvuII CAG'CTG
Cuts at: 2516 2931 3332 3359 2516
Size: 415 401 27 5815
Fragments arranged by size:
5815 415 401 27
SacI G_AGCT'C
Cuts at: 3846 3846
Size: 6658
SalI G'TCGA_C
Cuts at: 1936 2345 4000 1936
Size: 409 1655 4594
Fragments arranged by size:
4594 1655 409
SanDI GG'GwC_CC
Cuts at: 3780 3780
Size: 6658
SapI GCTCTTCn'nnn_
Cuts at: 2568 3834 2568
Size: 1266 5392
SbfI CC_TGCA'GG
Cuts at: 2096 4010 2096
Size: 1914 4744
ScaI AGT'ACT
Cuts at: 4906 6618 4906
Size: 1712 4946
SfcI C'TryA_G
Cuts at: 826 1045 2031 2092 2212 3261 3288 4006 5141
Size: 219 986 61 120 1049 27 718 1135
Cuts at: 5141 5822 6013 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1049 986 718 681 219 191
120 61 27
SfoI GGC'GCC
Cuts at: 2997 3237 2997
Size: 240 6418
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6658
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6658
SmaI CCC'GGG
Cuts at: 1872 3245 3985 1872
Size: 1373 740 4545
Fragments arranged by size:
4545 1373 740
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2132 3707 4762 5663 5925 820
Size: 197 926 189 1575 1055 901 262 1553
Fragments arranged by size:
1575 1553 1055 926 901 262 197 189
SnaBI TAC'GTA
Cuts at: 493 4449 493
Size: 3956 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6658
SphI G_CATG'C
Cuts at: 1132 4016 1132
Size: 2884 3774
SspI AAT'ATT
Cuts at: 5 52 4582 5
Size: 47 4530 2081
Fragments arranged by size:
4530 2081 47
StuI AGG'CCT
Cuts at: 1517 2054 3197 1517
Size: 537 1143 4978
Fragments arranged by size:
4978 1143 537
StyI C'CwwG_G
Cuts at: 513 1065 2004 2018 2879 3210 3852 4097 513
Size: 552 939 14 861 331 642 245 3074
Fragments arranged by size:
3074 939 861 642 552 331 245 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2254 3782 4685 4702 4855 5040 5485
Size: 1255 674 1528 903 17 153 185 445
Cuts at: 5485 325
Size: 1498
Fragments arranged by size:
1528 1498 1255 903 674 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2103 4904 6616
Size: 274 80 33 51 1443 126 2801 1712
Cuts at: 6616 96
Size: 138
Fragments arranged by size:
2801 1712 1443 274 138 126 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6618 6650 6618
Size: 32 6626
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6658
XbaI T'CTAG_A
Cuts at: 3994 3994
Size: 6658
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6658
XmaI C'CCGG_G
Cuts at: 1870 3243 3983 1870
Size: 1373 740 4545
Fragments arranged by size:
4545 1373 740
XmnI GAAnn'nnTTC
Cuts at: 4787 4787
Size: 6658
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4466 276
Size: 53 83 186 936 2932 2468
Fragments arranged by size:
2932 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BfrBI BsiWI BsmBI BstZ17I DraIII
EcoRV FalI FspAI NruI NsiI PacI PfoI PspXI
PsrI RsrII SacII SexAI SfiI SrfI SwaI XcmI