Restriction map for
pFN21AB8354
(Circular) (Six-base) MAPSORT of: pFN21AB8354.seq Check: 5037 from: 1 to: 6076
pFN21AB8354 6076 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3886 278
Size: 53 83 186 936 2350 2468
Fragments arranged by size:
2468 2350 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2835 3419 1937
Size: 898 584 4594
Fragments arranged by size:
4594 898 584
Acc65I G'GTAC_C
Cuts at: 3397 3397
Size: 6076
AclI AA'CG_TT
Cuts at: 1563 4203 4576 1563
Size: 2640 373 3063
Fragments arranged by size:
3063 2640 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3008 3288 4139 5523 5791 1632
Size: 1376 280 851 1384 268 1917
Fragments arranged by size:
1917 1384 1376 851 280 268
AflII C'TTAA_G
Cuts at: 820 1017 2744 820
Size: 197 1727 4152
Fragments arranged by size:
4152 1727 197
AflIII A'CryG_T
Cuts at: 1203 3237 4975 5952 1203
Size: 2034 1738 977 1327
Fragments arranged by size:
2034 1738 1327 977
AgeI A'CCGG_T
Cuts at: 4907 4907
Size: 6076
AhdI GACnn_n'nnGTC
Cuts at: 4805 4805
Size: 6076
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6076
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2873 1184
Size: 1689 4387
AlwNI CAG_nnn'CTG
Cuts at: 2054 3819 5391 2054
Size: 1765 1572 2739
Fragments arranged by size:
2739 1765 1572
ApaLI G'TGCA_C
Cuts at: 4133 5289 4133
Size: 1156 4920
ApoI r'AATT_y
Cuts at: 1493 2367 2699 3385 3611 3763 5872 1493
Size: 874 332 686 226 152 2109 1697
Fragments arranged by size:
2109 1697 874 686 332 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4630 160
Size: 872 3598 1606
Fragments arranged by size:
3598 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6076
AvaI C'yCGr_G
Cuts at: 1870 1943 3401 1870
Size: 73 1458 4545
Fragments arranged by size:
4545 1458 73
BamHI G'GATC_C
Cuts at: 3406 3773 3406
Size: 367 5709
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2021 3397 4852 618
Size: 325 217 627 234 1376 1455 1842
Fragments arranged by size:
1842 1455 1376 627 325 234 217
BanII G_rGCy'C
Cuts at: 1391 2406 3395 1391
Size: 1015 989 4072
Fragments arranged by size:
4072 1015 989
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2592 3305 928
Size: 971 693 713 3699
Fragments arranged by size:
3699 971 713 693
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2328 2362 4267 4301 1903
Size: 34 391 34 1905 34 3678
Fragments arranged by size:
3678 1905 391 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2776 3968 5178 2776
Size: 1192 1210 3674
Fragments arranged by size:
3674 1210 1192
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6076
BfrBI ATG'CAT
Cuts at: 2339 2339
Size: 6076
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4687 136
Size: 107 122 71 631 605 3015 1525
Fragments arranged by size:
3015 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3174 6071 3174
Size: 2897 3179
BlpI GC'TnA_GC
Cuts at: 2061 2721 3493 2061
Size: 660 772 4644
Fragments arranged by size:
4644 772 660
Bme1580I G_kGCm'C
Cuts at: 1790 4137 5293 1790
Size: 2347 1156 2573
Fragments arranged by size:
2573 2347 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6076
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3807 4765 442
Size: 416 53 83 399 24 2390 958 1753
Fragments arranged by size:
2390 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6076
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2642 4736 1239
Size: 1403 2094 2579
Fragments arranged by size:
2579 2094 1403
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4201 5066 5364 4201
Size: 865 298 4913
Fragments arranged by size:
4913 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4739 882
Size: 617 3240 2219
Fragments arranged by size:
3240 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3373 3867 493
Size: 711 2169 494 2702
Fragments arranged by size:
2702 2169 711 494
BsaBI GATnn'nnATC
Cuts at: 6070 6070
Size: 6076
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3883 4265 4900 275
Size: 53 83 186 936 2350 382 635 1451
Fragments arranged by size:
2350 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2451 4509 4907 5181 5328 5739 2451
Size: 2058 398 274 147 411 2788
Fragments arranged by size:
2788 2058 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2145 2175 2490 2520 1178
Size: 30 165 30 742 30 315 30 4734
Fragments arranged by size:
4734 742 315 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6076
BseYI C'CCAG_C
Cuts at: 3084 5279 5775 3084
Size: 2195 496 3385
Fragments arranged by size:
3385 2195 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5503
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3526 4287 5315 1540
Size: 275 186 1525 761 1028 2301
Fragments arranged by size:
2301 1525 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2406 3207 4137 4222 5293 2406
Size: 801 930 85 1071 3189
Fragments arranged by size:
3189 1071 930 801 85
BsmI GAATG_Cn'
Cuts at: 2383 2564 3023 3587 3680 2383
Size: 181 459 564 93 4779
Fragments arranged by size:
4779 564 459 181 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2406 3207 3395 4137 4222 5293 1391
Size: 399 616 801 188 742 85 1071 2174
Fragments arranged by size:
2174 1071 801 742 616 399 188 85
BspEI T'CCGG_A
Cuts at: 2451 5739 2451
Size: 3288 2788
BspHI T'CATG_A
Cuts at: 2215 3093 3965 5695 2215
Size: 878 872 1730 2596
Fragments arranged by size:
2596 1730 878 872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3431 844
Size: 2587 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3214 3963 1394
Size: 429 1391 749 3507
Fragments arranged by size:
3507 1391 749 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3294 3351 4571 4745 65
Size: 1183 2046 57 1220 174 1396
Fragments arranged by size:
2046 1396 1220 1183 174 57
BsrFI r'CCGG_y
Cuts at: 1700 1810 4720 4907 1700
Size: 110 2910 187 2869
Fragments arranged by size:
2910 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6076
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6076
BssSI C'ACGA_G
Cuts at: 4136 5148 4136
Size: 1012 5064
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6076
BstBI TT'CG_AA
Cuts at: 2754 4880 2754
Size: 2126 3950
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6076
BstYI r'GATC_y
Cuts at: 1972 3174 3406 3773 4158 4175 5627 5713 5725
Size: 1202 232 367 385 17 1452 86 12
Cuts at: 5725 6071 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1202 385 367 346 232 86
17 12
BstZ17I GTA'TAC
Cuts at: 2836 2836
Size: 6076
Bsu36I CC'TnA_GG
Cuts at: 3363 3363
Size: 6076
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5524
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5337
Fragments arranged by size:
5337 683 56
BtsI GCAGTG_nn'
Cuts at: 3592 4386 4406 3592
Size: 794 20 5262
Fragments arranged by size:
5262 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6076
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6041
DraI TTT'AAA
Cuts at: 3380 3380
Size: 6076
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5083 809
Size: 507 3767 1802
Fragments arranged by size:
3767 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2973 3523 4412
Size: 54 1425 50 275 18 1143 550 889
Cuts at: 4412 6041 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1143 889 550 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3523 1537
Size: 275 1711 4090
Fragments arranged by size:
4090 1711 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3267 3348 4006 1353
Size: 1914 81 658 3423
Fragments arranged by size:
3423 1914 658 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4663 5037 5183 387
Size: 1064 138 3074 374 146 1280
Fragments arranged by size:
3074 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2404 2404
Size: 6076
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2642 3008 3288 4139 4736 5523 5791
Size: 393 1010 366 280 851 597 787 268
Cuts at: 5791 1239
Size: 1524
Fragments arranged by size:
1524 1010 851 787 597 393 366 280
268
EcoO109I rG'GnC_Cy
Cuts at: 3031 3031
Size: 6076
EcoRI G'AATT_C
Cuts at: 3385 3763 3385
Size: 378 5698
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2759 3139 2759
Size: 380 5696
FspI TGC'GCA
Cuts at: 4582 4582
Size: 6076
HaeII r_GCGC'y
Cuts at: 1678 5223 1678
Size: 3545 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2066 2098 2433 2465 3092
Size: 32 135 32 494 32 335 32 627
Cuts at: 3092 3124 3159 3191 4717 4749 4791 4823 1373
Size: 32 35 32 1526 32 42 32 2626
Fragments arranged by size:
2626 1526 627 494 335 135 42 35
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3420 3666 669
Size: 988 281 1482 246 3079
Fragments arranged by size:
3079 1482 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2934 2990 748
Size: 2186 56 3834
Fragments arranged by size:
3834 2186 56
HpaI GTT'AAC
Cuts at: 3666 3666
Size: 6076
KpnI G_GTAC'C
Cuts at: 3401 3401
Size: 6076
MfeI C'AATT_G
Cuts at: 2601 3675 2601
Size: 1074 5002
MluI A'CGCG_T
Cuts at: 5952 5952
Size: 6076
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5190 5374 1305
Size: 3885 184 2007
Fragments arranged by size:
3885 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2975 10
Size: 54 1425 343 1143 3111
Fragments arranged by size:
3111 1425 1143 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2282 4034 4393 4552 518
Size: 648 1116 1752 359 159 2042
Fragments arranged by size:
2042 1752 1116 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2529 2635 3088 3492 4169 5317 5562 1606
Size: 923 106 453 404 677 1148 245 2120
Fragments arranged by size:
2120 1148 923 677 453 404 245 106
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5524
NdeI CA'TA_TG
Cuts at: 387 2341 387
Size: 1954 4122
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6076
NotI GC'GGCC_GC
Cuts at: 3523 3523
Size: 6076
NsiI A_TGCA'T
Cuts at: 2341 2341
Size: 6076
NspI r_CATG'y
Cuts at: 1132 1229 2860 3241 3434 4979 1132
Size: 97 1631 381 193 1545 2229
Fragments arranged by size:
2229 1631 1545 381 193 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6076
PciI A'CATG_T
Cuts at: 3237 4975 3237
Size: 1738 4338
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2672 1108
Size: 1564 4512
PmeI GTTT'AAAC
Cuts at: 3380 3380
Size: 6076
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4145 4177 5658 5690 4145
Size: 32 1481 32 4531
Fragments arranged by size:
4531 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6076
PsiI TTA'TAA
Cuts at: 3646 3646
Size: 6076
PstI C_TGCA'G
Cuts at: 830 2555 3174 3428 830
Size: 1725 619 254 3478
Fragments arranged by size:
3478 1725 619 254
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6076
PvuII CAG'CTG
Cuts at: 3088 3088
Size: 6076
SacI G_AGCT'C
Cuts at: 2406 2406
Size: 6076
SalI G'TCGA_C
Cuts at: 1936 3418 1936
Size: 1482 4594
SbfI CC_TGCA'GG
Cuts at: 3428 3428
Size: 6076
ScaI AGT'ACT
Cuts at: 4324 6036 4324
Size: 1712 4364
SfcI C'TryA_G
Cuts at: 826 1045 2551 3170 3424 4559 5240 5431 826
Size: 219 1506 619 254 1135 681 191 1471
Fragments arranged by size:
1506 1471 1135 681 619 254 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6076
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6076
SmaI CCC'GGG
Cuts at: 1872 3403 1872
Size: 1531 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2744 4180 5081 5343 820
Size: 197 926 801 1436 901 262 1553
Fragments arranged by size:
1553 1436 926 901 801 262 197
SnaBI TAC'GTA
Cuts at: 493 3867 493
Size: 3374 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6076
SphI G_CATG'C
Cuts at: 1132 3434 1132
Size: 2302 3774
SspI AAT'ATT
Cuts at: 5 52 4000 5
Size: 47 3948 2081
Fragments arranged by size:
3948 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6076
StyI C'CwwG_G
Cuts at: 513 1065 3034 3114 3198 3267 3515 513
Size: 552 1969 80 84 69 248 3074
Fragments arranged by size:
3074 1969 552 248 84 80 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4103 4120 4273 4458 4903 325
Size: 1255 2523 17 153 185 445 1498
Fragments arranged by size:
2523 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4322 6034 96
Size: 274 80 33 51 1443 2345 1712 138
Fragments arranged by size:
2345 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6036 6068 6036
Size: 32 6044
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6076
XbaI T'CTAG_A
Cuts at: 3412 3412
Size: 6076
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2794 3286 2794
Size: 492 5584
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6076
XmaI C'CCGG_G
Cuts at: 1870 3401 1870
Size: 1531 4545
XmnI GAAnn'nnTTC
Cuts at: 2946 4205 2946
Size: 1259 4817
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3884 276
Size: 53 83 186 936 2350 2468
Fragments arranged by size:
2468 2350 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI DraIII EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI