Restriction map for
pFN21AB8353
(Circular) (Six-base) MAPSORT of: pFN21AB8353.seq Check: 5192 from: 1 to: 5746
pFN21AB8353 5746 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2760 2760
Size: 5746
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3556 278
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2613 2769 3089 1937
Size: 676 156 320 4594
Fragments arranged by size:
4594 676 320 156
Acc65I G'GTAC_C
Cuts at: 2913 3067 2913
Size: 154 5592
AclI AA'CG_TT
Cuts at: 1563 3873 4246 1563
Size: 2310 373 3063
Fragments arranged by size:
3063 2310 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3809 5193 5461 1632
Size: 2177 1384 268 1917
Fragments arranged by size:
2177 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5549
AflIII A'CryG_T
Cuts at: 1203 4645 5622 1203
Size: 3442 977 1327
Fragments arranged by size:
3442 1327 977
AgeI A'CCGG_T
Cuts at: 4577 4577
Size: 5746
AhdI GACnn_n'nnGTC
Cuts at: 4475 4475
Size: 5746
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5746
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5746
AlwNI CAG_nnn'CTG
Cuts at: 3489 5061 3489
Size: 1572 4174
ApaLI G'TGCA_C
Cuts at: 2225 3803 4959 2225
Size: 1578 1156 3012
Fragments arranged by size:
3012 1578 1156
ApoI r'AATT_y
Cuts at: 1493 3055 3281 3433 5542 1493
Size: 1562 226 152 2109 1697
Fragments arranged by size:
2109 1697 1562 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4300 160
Size: 872 3268 1606
Fragments arranged by size:
3268 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
AvaI C'yCGr_G
Cuts at: 1870 1943 2792 3071 1870
Size: 73 849 279 4545
Fragments arranged by size:
4545 849 279 73
BamHI G'GATC_C
Cuts at: 3076 3443 3076
Size: 367 5379
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2780 2884 2913 3067 4522
Size: 325 217 627 993 104 29 154 1455
Cuts at: 4522 618
Size: 1842
Fragments arranged by size:
1842 1455 993 627 325 217 154 104
29
BanII G_rGCy'C
Cuts at: 1391 2253 2976 3065 1391
Size: 862 723 89 4072
Fragments arranged by size:
4072 862 723 89
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4775
BbvCI CC'TCA_GC
Cuts at: 2336 2336
Size: 5746
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3937 3971 1903
Size: 34 50 34 1916 34 3678
Fragments arranged by size:
3678 1916 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3638 4848 3638
Size: 1210 4536
BclI T'GATC_A
Cuts at: 1548 2139 1548
Size: 591 5155
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2654 2832 4357
Size: 107 122 71 631 605 982 178 1525
Cuts at: 4357 136
Size: 1525
Fragments arranged by size:
1525 1525 982 631 605 178 122 107
71
BglII A'GATC_T
Cuts at: 5741 5741
Size: 5746
BlpI GC'TnA_GC
Cuts at: 3163 3163
Size: 5746
Bme1580I G_kGCm'C
Cuts at: 1790 2229 2690 2889 3045 3807 4963 1790
Size: 439 461 199 156 762 1156 2573
Fragments arranged by size:
2573 1156 762 461 439 199 156
BmgBI CAC'GTC
Cuts at: 1318 2761 1318
Size: 1443 4303
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3477 4435 442
Size: 416 53 83 399 24 2060 958 1753
Fragments arranged by size:
2060 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5746
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2536 2536
Size: 5746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2117 2422 2506 2699 2726 4406 1239
Size: 878 305 84 193 27 1680 2579
Fragments arranged by size:
2579 1680 878 305 193 84 27
Bpu10I CC'TnA_GC
Cuts at: 2336 2336
Size: 5746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2237 3871 4736 5034 2237
Size: 1634 865 298 2949
Fragments arranged by size:
2949 1634 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4409 882
Size: 617 2910 2219
Fragments arranged by size:
2910 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3537 493
Size: 711 2333 2702
Fragments arranged by size:
2702 2333 711
BsaBI GATnn'nnATC
Cuts at: 2550 5740 2550
Size: 3190 2556
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3553 3935 4570 275
Size: 53 83 186 936 2020 382 635 1451
Fragments arranged by size:
2020 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2862 4179 4577 4851 4998 5409 2862
Size: 1317 398 274 147 411 3199
Fragments arranged by size:
3199 1317 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2420 2450 2666 2696 1178
Size: 30 165 30 1017 30 216 30 4228
Fragments arranged by size:
4228 1017 216 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2222 1186
Size: 1036 4710
BseYI C'CCAG_C
Cuts at: 2019 2400 2827 2993 4949 5445 2019
Size: 381 427 166 1956 496 2320
Fragments arranged by size:
2320 1956 496 427 381 166
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2342 1163
Size: 573 606 4567
Fragments arranged by size:
4567 606 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2446 3196 3957 4985 1540
Size: 275 186 445 750 761 1028 2301
Fragments arranged by size:
2301 1028 761 750 445 275 186
BsiHKAI G_wGCw'C
Cuts at: 2229 2253 2751 3807 3892 4963 2229
Size: 24 498 1056 85 1071 3012
Fragments arranged by size:
3012 1071 1056 498 85 24
BsmI GAATG_Cn'
Cuts at: 3257 3350 3257
Size: 93 5653
BsmBI CGTCTCn'nnnn_
Cuts at: 3004 3004
Size: 5746
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2229 2253 2690 2751 2889 2976 3045
Size: 399 439 24 437 61 138 87 69
Cuts at: 3045 3065 3807 3892 4963 1391
Size: 20 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 439 437 399 138 87
85 69 61 24 20
BspEI T'CCGG_A
Cuts at: 2862 5409 2862
Size: 2547 3199
BspHI T'CATG_A
Cuts at: 3635 5365 3635
Size: 1730 4016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2223 2760 3101 844
Size: 1379 537 341 3489
Fragments arranged by size:
3489 1379 537 341
BsrBI CCG'CTC
Cuts at: 1394 1823 3633 1394
Size: 429 1810 3507
Fragments arranged by size:
3507 1810 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2566 2937 4241 4415 65
Size: 1183 1318 371 1304 174 1396
Fragments arranged by size:
1396 1318 1304 1183 371 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4390 4577 1700
Size: 110 2580 187 2869
Fragments arranged by size:
2869 2580 187 110
BsrGI T'GTAC_A
Cuts at: 96 2598 96
Size: 2502 3244
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5746
BssSI C'ACGA_G
Cuts at: 2230 3806 4818 2230
Size: 1576 1012 3158
Fragments arranged by size:
3158 1576 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5746
BstBI TT'CG_AA
Cuts at: 4550 4550
Size: 5746
BstEII G'GTnAC_C
Cuts at: 1184 2899 1184
Size: 1715 4031
BstXI CCAn_nnnn'nTGG
Cuts at: 2413 2896 2413
Size: 483 5263
BstYI r'GATC_y
Cuts at: 1972 3076 3443 3828 3845 5297 5383 5395 5741
Size: 1104 367 385 17 1452 86 12 346
Cuts at: 5741 1972
Size: 1977
Fragments arranged by size:
1977 1452 1104 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2770 2770
Size: 5746
Bsu36I CC'TnA_GG
Cuts at: 2905 2905
Size: 5746
BtgI C'CryG_G
Cuts at: 513 1065 2446 2872 513
Size: 552 1381 426 3387
Fragments arranged by size:
3387 1381 552 426
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5007
Fragments arranged by size:
5007 683 56
BtsI GCAGTG_nn'
Cuts at: 2431 3045 3262 4056 4076 2431
Size: 614 217 794 20 4101
Fragments arranged by size:
4101 794 614 217 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5711
DraI TTT'AAA
Cuts at: 3050 3050
Size: 5746
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4753 809
Size: 507 3437 1802
Fragments arranged by size:
3437 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2051 2875 3193
Size: 54 1425 50 275 18 221 824 318
Cuts at: 3193 4082 5711 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 824 318 275 221 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3193 1537
Size: 275 1381 4090
Fragments arranged by size:
4090 1381 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2151 3676 1353
Size: 798 1525 3423
Fragments arranged by size:
3423 1525 798
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2680 4333 4707 4853 387
Size: 1064 138 1091 1653 374 146 1280
Fragments arranged by size:
1653 1280 1091 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2251 2251
Size: 5746
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2422 2506 2699 2726 3809 4406
Size: 393 485 305 84 193 27 1083 597
Cuts at: 4406 5193 5461 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1083 787 597 485 393 305 268
193 84 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2711 2711
Size: 5746
EcoO109I rG'GnC_Cy
Cuts at: 2212 2340 2212
Size: 128 5618
EcoRI G'AATT_C
Cuts at: 3055 3433 3055
Size: 378 5368
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2180 2278 2180
Size: 98 5648
FspI TGC'GCA
Cuts at: 4252 4252
Size: 5746
HaeII r_GCGC'y
Cuts at: 1678 2239 4893 1678
Size: 561 2654 2531
Fragments arranged by size:
2654 2531 561
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2224 2256 4387 4419 4461
Size: 32 135 32 652 32 2131 32 42
Cuts at: 4461 4493 1373
Size: 32 2626
Fragments arranged by size:
2626 2131 652 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3090 3336 669
Size: 988 281 1152 246 3079
Fragments arranged by size:
3079 1152 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2162 748
Size: 1414 4332
HpaI GTT'AAC
Cuts at: 3336 3336
Size: 5746
KpnI G_GTAC'C
Cuts at: 2917 3071 2917
Size: 154 5592
MfeI C'AATT_G
Cuts at: 3345 3345
Size: 5746
MluI A'CGCG_T
Cuts at: 5622 5622
Size: 5746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4860 5044 1305
Size: 3555 184 2007
Fragments arranged by size:
3555 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2053 10
Size: 54 1425 343 221 3703
Fragments arranged by size:
3703 1425 343 221 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2087 2183 2411 2894 3704 4063 4222
Size: 648 921 96 228 483 810 359 159
Cuts at: 4222 518
Size: 2042
Fragments arranged by size:
2042 921 810 648 483 359 228 159
96
MspA1I CmG'CkG
Cuts at: 1606 2023 2831 3025 3162 3839 4987 5232 1606
Size: 417 808 194 137 677 1148 245 2120
Fragments arranged by size:
2120 1148 808 677 417 245 194 137
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5194
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5746
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5746
NotI GC'GGCC_GC
Cuts at: 3193 3193
Size: 5746
NspI r_CATG'y
Cuts at: 1132 1229 2036 2182 3104 4649 1132
Size: 97 807 146 922 1545 2229
Fragments arranged by size:
2229 1545 922 807 146 97
PasI CC'CwG_GG
Cuts at: 1348 2633 1348
Size: 1285 4461
PciI A'CATG_T
Cuts at: 4645 4645
Size: 5746
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5746
PfoI T'CCnGG_A
Cuts at: 2742 2742
Size: 5746
PmeI GTTT'AAAC
Cuts at: 3050 3050
Size: 5746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2843 2875 3815 3847 5328 5360 2843
Size: 32 940 32 1481 32 3229
Fragments arranged by size:
3229 1481 940 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2212 2212
Size: 5746
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5746
PsiI TTA'TAA
Cuts at: 3316 3316
Size: 5746
PspXI vC'TCGA_Gb
Cuts at: 2792 2792
Size: 5746
PstI C_TGCA'G
Cuts at: 830 3040 3098 830
Size: 2210 58 3478
Fragments arranged by size:
3478 2210 58
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5746
PvuII CAG'CTG
Cuts at: 2023 2023
Size: 5746
SacI G_AGCT'C
Cuts at: 2253 2253
Size: 5746
SalI G'TCGA_C
Cuts at: 1936 3088 1936
Size: 1152 4594
SbfI CC_TGCA'GG
Cuts at: 3098 3098
Size: 5746
ScaI AGT'ACT
Cuts at: 3994 5706 3994
Size: 1712 4034
SfcI C'TryA_G
Cuts at: 826 1045 3036 3094 4229 4910 5101 826
Size: 219 1991 58 1135 681 191 1471
Fragments arranged by size:
1991 1471 1135 681 219 191 58
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5746
SmaI CCC'GGG
Cuts at: 1872 3073 1872
Size: 1201 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2252 2792 3850 4751 5013 820
Size: 197 926 309 540 1058 901 262 1553
Fragments arranged by size:
1553 1058 926 901 540 309 262 197
SnaBI TAC'GTA
Cuts at: 493 3537 493
Size: 3044 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5746
SphI G_CATG'C
Cuts at: 1132 2182 3104 1132
Size: 1050 922 3774
Fragments arranged by size:
3774 1050 922
SspI AAT'ATT
Cuts at: 5 52 3670 5
Size: 47 3618 2081
Fragments arranged by size:
3618 2081 47
StuI AGG'CCT
Cuts at: 1517 2201 1517
Size: 684 5062
StyI C'CwwG_G
Cuts at: 513 1065 2806 3185 513
Size: 552 1741 379 3074
Fragments arranged by size:
3074 1741 552 379
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3007 3773 3790 3943 4128 4573 325
Size: 1255 1427 766 17 153 185 445 1498
Fragments arranged by size:
1498 1427 1255 766 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2061 2598 3992
Size: 274 80 33 51 1443 84 537 1394
Cuts at: 3992 5704 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1394 537 274 138 84 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2420 2452 2666 2698 2857 2889 5706 5738 2420
Size: 32 214 32 159 32 2817 32 2428
Fragments arranged by size:
2817 2428 214 159 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5746
XbaI T'CTAG_A
Cuts at: 3082 3082
Size: 5746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2046 2558 2046
Size: 512 5234
XhoI C'TCGA_G
Cuts at: 1943 2792 1943
Size: 849 4897
XmaI C'CCGG_G
Cuts at: 1870 3071 1870
Size: 1201 4545
XmnI GAAnn'nnTTC
Cuts at: 3875 3875
Size: 5746
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3554 276
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI DraIII EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI