Restriction map for
pFN21AB8352
(Circular) (Six-base) MAPSORT of: pFN21AB8352.seq Check: 7541 from: 1 to: 6784
pFN21AB8352 6784 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2358 2358
Size: 6784
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4594 278
Size: 53 83 186 936 3058 2468
Fragments arranged by size:
3058 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4127 1937
Size: 2190 4594
Acc65I G'GTAC_C
Cuts at: 4105 4105
Size: 6784
AclI AA'CG_TT
Cuts at: 1563 4911 5284 1563
Size: 3348 373 3063
Fragments arranged by size:
3348 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2825 3289 4847 6231 6499 1632
Size: 1193 464 1558 1384 268 1917
Fragments arranged by size:
1917 1558 1384 1193 464 268
AfeI AGC'GCT
Cuts at: 2264 2264
Size: 6784
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6587
AflIII A'CryG_T
Cuts at: 1203 3588 5683 6660 1203
Size: 2385 2095 977 1327
Fragments arranged by size:
2385 2095 1327 977
AgeI A'CCGG_T
Cuts at: 5615 5615
Size: 6784
AhdI GACnn_n'nnGTC
Cuts at: 5513 5513
Size: 6784
AleI CACnn'nnGTG
Cuts at: 1166 3869 1166
Size: 2703 4081
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6784
AlwNI CAG_nnn'CTG
Cuts at: 2549 3028 3637 3964 4006 4527 6099 2549
Size: 479 609 327 42 521 1572 3234
Fragments arranged by size:
3234 1572 609 521 479 327 42
ApaI G_GGCC'C
Cuts at: 2024 3988 4059 2024
Size: 1964 71 4749
Fragments arranged by size:
4749 1964 71
ApaLI G'TGCA_C
Cuts at: 2333 4841 5997 2333
Size: 2508 1156 3120
Fragments arranged by size:
3120 2508 1156
ApoI r'AATT_y
Cuts at: 1493 4073 4093 4319 4471 6580 1493
Size: 2580 20 226 152 2109 1697
Fragments arranged by size:
2580 2109 1697 226 152 20
AseI AT'TA_AT
Cuts at: 160 1032 5338 160
Size: 872 4306 1606
Fragments arranged by size:
4306 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6784
AvaI C'yCGr_G
Cuts at: 1870 1943 2023 2428 2700 3051 3257 4109 1870
Size: 73 80 405 272 351 206 852 4545
Fragments arranged by size:
4545 852 405 351 272 206 80 73
AvrII C'CTAG_G
Cuts at: 3721 3721
Size: 6784
BamHI G'GATC_C
Cuts at: 3854 4114 4481 3854
Size: 260 367 6157
Fragments arranged by size:
6157 367 260
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2045 2068 2130 2188 2388
Size: 325 217 627 258 23 62 58 200
Cuts at: 2388 2417 2705 3239 3365 4105 5560 618
Size: 29 288 534 126 740 1455 1842
Fragments arranged by size:
1842 1455 740 627 534 325 288 258
217 200 126 62 58 29 23
BanII G_rGCy'C
Cuts at: 1391 2024 2034 2121 2184 2477 2556 2639 2662
Size: 633 10 87 63 293 79 83 23
Cuts at: 2662 2730 3610 3988 4059 4103 1391
Size: 68 880 378 71 44 4072
Fragments arranged by size:
4072 880 633 378 293 87 83 79
71 68 63 44 23 10
BbeI G_GCGC'C
Cuts at: 2049 2192 2049
Size: 143 6641
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3362 928
Size: 971 1463 4350
Fragments arranged by size:
4350 1463 971
BbvCI CC'TCA_GC
Cuts at: 2494 3021 3408 2494
Size: 527 387 5870
Fragments arranged by size:
5870 527 387
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3813 3847 3888 3922 4975 5009 1903
Size: 34 1876 34 41 34 1053 34 3678
Fragments arranged by size:
3678 1876 1053 41 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4676 5886 4676
Size: 1210 5574
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6784
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2321 3726 5395
Size: 107 122 71 631 605 649 1405 1669
Cuts at: 5395 136
Size: 1525
Fragments arranged by size:
1669 1525 1405 649 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3498 6779 3498
Size: 3281 3503
BlpI GC'TnA_GC
Cuts at: 4201 4201
Size: 6784
Bme1580I G_kGCm'C
Cuts at: 1790 2024 2337 2708 3242 3664 3988 4059 4845
Size: 234 313 371 534 422 324 71 786
Cuts at: 4845 6001 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 786 534 422 371 324 313
234 71
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6784
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4515 5473 442
Size: 416 53 83 399 24 3098 958 1753
Fragments arranged by size:
3098 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6784
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2135 2491 2586 2843 2990 3438 5444 1239
Size: 896 356 95 257 147 448 2006 2579
Fragments arranged by size:
2579 2006 896 448 356 257 147 95
Bpu10I CC'TnA_GC
Cuts at: 2494 2550 3021 3408 2494
Size: 56 471 387 5870
Fragments arranged by size:
5870 471 387 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2714 4909 5774 6072 2714
Size: 2195 865 298 3426
Fragments arranged by size:
3426 2195 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3323 5447 882
Size: 617 1824 2124 2219
Fragments arranged by size:
2219 2124 1824 617
BsaAI yAC'GTr
Cuts at: 493 1204 3589 4575 493
Size: 711 2385 986 2702
Fragments arranged by size:
2702 2385 986 711
BsaBI GATnn'nnATC
Cuts at: 3929 6778 3929
Size: 2849 3935
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2046 2109 2189 2195
Size: 53 83 186 936 513 63 80 6
Cuts at: 2195 2202 4591 4973 5608 275
Size: 7 2389 382 635 1451
Fragments arranged by size:
2389 1451 936 635 513 382 186 83
80 63 53 7 6
BsaWI w'CCGG_w
Cuts at: 5217 5615 5889 6036 6447 5217
Size: 398 274 147 411 5554
Fragments arranged by size:
5554 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2855 2885 1178
Size: 30 165 30 1452 30 5077
Fragments arranged by size:
5077 1452 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2738 3275 3416 3425 1186
Size: 1552 537 141 9 4545
Fragments arranged by size:
4545 1552 537 141 9
BseYI C'CCAG_C
Cuts at: 2049 2435 2515 3083 3148 3197 3781 3837 5987
Size: 386 80 568 65 49 584 56 2150
Cuts at: 5987 6483 2049
Size: 496 2350
Fragments arranged by size:
2350 2150 584 568 496 386 80 65
56 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2433 3524 1163
Size: 573 697 1091 4423
Fragments arranged by size:
4423 1091 697 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4234 4995 6023 1540
Size: 275 186 2233 761 1028 2301
Fragments arranged by size:
2301 2233 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2337 2556 2639 2730 2920 3446 4845 4930 6001
Size: 219 83 91 190 526 1399 85 1071
Cuts at: 6001 2337
Size: 3120
Fragments arranged by size:
3120 1399 1071 526 219 190 91 85
83
BsmI GAATG_Cn'
Cuts at: 4295 4388 4295
Size: 93 6691
BsmBI CGTCTCn'nnnn_
Cuts at: 2984 2984
Size: 6784
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2034 2121 2184 2337 2477 2556
Size: 399 234 10 87 63 153 140 79
Cuts at: 2556 2639 2662 2708 2730 2920 3242 3446 3610
Size: 83 23 46 22 190 322 204 164
Cuts at: 3610 3664 3988 4059 4103 4845 4930 6001 1391
Size: 54 324 71 44 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 324 322 234 204
190 164 153 140 87 85 83 79
71 63 54 46 44 23 22 10
BspEI T'CCGG_A
Cuts at: 6447 6447
Size: 6784
BspHI T'CATG_A
Cuts at: 4673 6403 4673
Size: 1730 5054
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2358 3261 3446 4139 844
Size: 1514 903 185 693 3489
Fragments arranged by size:
3489 1514 903 693 185
BsrBI CCG'CTC
Cuts at: 1394 1823 4671 1394
Size: 429 2848 3507
Fragments arranged by size:
3507 2848 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5279 5453 65
Size: 1183 4031 174 1396
Fragments arranged by size:
4031 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3800 5428 5615 1700
Size: 110 1990 1628 187 2869
Fragments arranged by size:
2869 1990 1628 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6784
BssHII G'CGCG_C
Cuts at: 1925 2174 1925
Size: 249 6535
BssSI C'ACGA_G
Cuts at: 2330 4844 5856 2330
Size: 2514 1012 3258
Fragments arranged by size:
3258 2514 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6784
BstBI TT'CG_AA
Cuts at: 5588 5588
Size: 6784
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6784
BstXI CCAn_nnnn'nTGG
Cuts at: 2141 3871 2141
Size: 1730 5054
BstYI r'GATC_y
Cuts at: 1972 3498 3854 4114 4481 4866 4883 6335 6421
Size: 1526 356 260 367 385 17 1452 86
Cuts at: 6421 6433 6779 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1526 1452 385 367 356 346 260
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3791 3810 3791
Size: 19 6765
BtgI C'CryG_G
Cuts at: 513 1065 2004 3094 3626 3702 3870 513
Size: 552 939 1090 532 76 168 3427
Fragments arranged by size:
3427 1090 939 552 532 168 76
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3472 487
Size: 683 56 2246 3799
Fragments arranged by size:
3799 2246 683 56
BtsI GCAGTG_nn'
Cuts at: 2421 2841 2983 3446 4300 5094 5114 2421
Size: 420 142 463 854 794 20 4091
Fragments arranged by size:
4091 854 794 463 420 142 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6784
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2877 2912 529
Size: 35 2313 35 4401
Fragments arranged by size:
4401 2313 35 35
DraI TTT'AAA
Cuts at: 4088 4088
Size: 6784
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5791 809
Size: 507 4475 1802
Fragments arranged by size:
4475 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2280 2322
Size: 54 1425 50 275 18 177 273 42
Cuts at: 2322 4231 5120 6749 8
Size: 1909 889 1629 43
Fragments arranged by size:
1909 1629 1425 889 275 273 177 54
50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 4231 1537
Size: 275 2419 4090
Fragments arranged by size:
4090 2419 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2792 3393 4714 1353
Size: 1439 601 1321 3423
Fragments arranged by size:
3423 1439 1321 601
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5371 5745 5891 387
Size: 1064 138 3782 374 146 1280
Fragments arranged by size:
3782 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2554 2637 2728 2554
Size: 83 91 6610
Fragments arranged by size:
6610 91 83
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2491 2586 2825 2843 2990 3289
Size: 393 503 356 95 239 18 147 299
Cuts at: 3289 3438 4847 5444 6231 6499 1239
Size: 149 1409 597 787 268 1524
Fragments arranged by size:
1524 1409 787 597 503 393 356 299
268 239 149 147 95 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2651 2855 3257 3794 3974 4040 2651
Size: 204 402 537 180 66 5395
Fragments arranged by size:
5395 537 402 204 180 66
EcoO109I rG'GnC_Cy
Cuts at: 2546 3689 3984 3985 4055 2546
Size: 1143 295 1 70 5275
Fragments arranged by size:
5275 1143 295 70 1
EcoRI G'AATT_C
Cuts at: 4093 4471 4093
Size: 378 6406
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2592 2863 2592
Size: 271 6513
FspI TGC'GCA
Cuts at: 5290 5290
Size: 6784
HaeII r_GCGC'y
Cuts at: 1678 2049 2192 2266 3647 5931 1678
Size: 371 143 74 1381 2284 2531
Fragments arranged by size:
2531 2284 1381 371 143 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2855 2887 3018 3050 3364
Size: 32 135 32 1283 32 131 32 314
Cuts at: 3364 3396 5425 5457 5499 5531 1373
Size: 32 2029 32 42 32 2626
Fragments arranged by size:
2626 2029 1283 314 135 131 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4128 4374 669
Size: 988 281 2190 246 3079
Fragments arranged by size:
3079 2190 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6784
HpaI GTT'AAC
Cuts at: 4374 4374
Size: 6784
KasI G'GCGC_C
Cuts at: 2045 2188 2045
Size: 143 6641
KpnI G_GTAC'C
Cuts at: 4109 4109
Size: 6784
MfeI C'AATT_G
Cuts at: 4383 4383
Size: 6784
MluI A'CGCG_T
Cuts at: 6660 6660
Size: 6784
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3138 5898 6082 1305
Size: 1833 2760 184 2007
Fragments arranged by size:
2760 2007 1833 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4962
Fragments arranged by size:
4962 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3491 3869 4742 5101 5260 518
Size: 648 2325 378 873 359 159 2042
Fragments arranged by size:
2325 2042 873 648 378 359 159
MspA1I CmG'CkG
Cuts at: 1606 2356 2869 3523 3679 4003 4200 4877 6025
Size: 750 513 654 156 324 197 677 1148
Cuts at: 6025 6270 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 750 677 654 513 324 245
197 156
NaeI GCC'GGC
Cuts at: 3802 3802
Size: 6784
NarI GG'CG_CC
Cuts at: 2046 2189 2046
Size: 143 6641
NcoI C'CATG_G
Cuts at: 513 1065 2004 3094 3626 513
Size: 552 939 1090 532 3671
Fragments arranged by size:
3671 1090 939 552 532
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6784
NgoMIV G'CCGG_C
Cuts at: 3800 3800
Size: 6784
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6784
NotI GC'GGCC_GC
Cuts at: 4231 4231
Size: 6784
NspI r_CATG'y
Cuts at: 1132 1229 4142 5687 1132
Size: 97 2913 1545 2229
Fragments arranged by size:
2913 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2076 1348
Size: 728 6056
PciI A'CATG_T
Cuts at: 5683 5683
Size: 6784
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3703 3964 1108
Size: 2595 261 3928
Fragments arranged by size:
3928 2595 261
PmeI GTTT'AAAC
Cuts at: 4088 4088
Size: 6784
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4853 4885 6366 6398 4853
Size: 32 1481 32 5239
Fragments arranged by size:
5239 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6784
PsiI TTA'TAA
Cuts at: 4354 4354
Size: 6784
PspOMI G'GGCC_C
Cuts at: 2020 3984 4055 2020
Size: 1964 71 4749
Fragments arranged by size:
4749 1964 71
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2915 2947 2915
Size: 32 6752
PstI C_TGCA'G
Cuts at: 830 2355 2655 2836 3469 3684 4002 4136 830
Size: 1525 300 181 633 215 318 134 3478
Fragments arranged by size:
3478 1525 633 318 300 215 181 134
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6784
PvuII CAG'CTG
Cuts at: 2356 2869 3523 3679 4003 2356
Size: 513 654 156 324 5137
Fragments arranged by size:
5137 654 513 324 156
SacI G_AGCT'C
Cuts at: 2556 2639 2730 2556
Size: 83 91 6610
Fragments arranged by size:
6610 91 83
SalI G'TCGA_C
Cuts at: 1936 4126 1936
Size: 2190 4594
SbfI CC_TGCA'GG
Cuts at: 2655 4136 2655
Size: 1481 5303
ScaI AGT'ACT
Cuts at: 5032 6744 5032
Size: 1712 5072
SexAI A'CCwGG_T
Cuts at: 3390 3390
Size: 6784
SfcI C'TryA_G
Cuts at: 826 1045 2351 2651 2832 3465 3680 3998 4132
Size: 219 1306 300 181 633 215 318 134
Cuts at: 4132 5267 5948 6139 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1306 1135 681 633 318 300 219
215 191 181 134
SfoI GGC'GCC
Cuts at: 2047 2190 2047
Size: 143 6641
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6784
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6784
SmaI CCC'GGG
Cuts at: 1872 2025 2702 3053 4111 1872
Size: 153 677 351 1058 4545
Fragments arranged by size:
4545 1058 677 351 153
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2729 4888 5789 6051 820
Size: 197 926 786 2159 901 262 1553
Fragments arranged by size:
2159 1553 926 901 786 262 197
SnaBI TAC'GTA
Cuts at: 493 4575 493
Size: 4082 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6784
SphI G_CATG'C
Cuts at: 1132 4142 1132
Size: 3010 3774
SrfI GCCC'GGGC
Cuts at: 3053 3053
Size: 6784
SspI AAT'ATT
Cuts at: 5 52 4708 5
Size: 47 4656 2081
Fragments arranged by size:
4656 2081 47
StuI AGG'CCT
Cuts at: 1517 2468 2999 3562 1517
Size: 951 531 563 4739
Fragments arranged by size:
4739 951 563 531
StyI C'CwwG_G
Cuts at: 513 1065 2004 3094 3333 3626 3721 3886 4223
Size: 552 939 1090 239 293 95 165 337
Cuts at: 4223 513
Size: 3074
Fragments arranged by size:
3074 1090 939 552 337 293 239 165
95
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2133 2258 2723 2742 2870 2901 3157
Size: 1255 553 125 465 19 128 31 256
Cuts at: 3157 3452 4811 4828 4981 5166 5611 325
Size: 295 1359 17 153 185 445 1498
Fragments arranged by size:
1498 1359 1255 553 465 445 295 256
185 153 128 125 31 19 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3264 5030 6742
Size: 274 80 33 51 1443 1287 1766 1712
Cuts at: 6742 96
Size: 138
Fragments arranged by size:
1766 1712 1443 1287 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6744 6776 6744
Size: 32 6752
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6784
XbaI T'CTAG_A
Cuts at: 4120 4120
Size: 6784
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2965 3012 3121 3700 2965
Size: 47 109 579 6049
Fragments arranged by size:
6049 579 109 47
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6784
XmaI C'CCGG_G
Cuts at: 1870 2023 2700 3051 4109 1870
Size: 153 677 351 1058 4545
Fragments arranged by size:
4545 1058 677 351 153
XmnI GAAnn'nnTTC
Cuts at: 2927 4913 2927
Size: 1986 4798
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4592 276
Size: 53 83 186 936 3058 2468
Fragments arranged by size:
3058 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BfrBI BplI BsiWI BstZ17I DraIII
EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI
PpuMI PspXI RsrII SacII SanDI SapI SfiI SwaI