Restriction map for
pFN21AB8351
(Circular) (Six-base) MAPSORT of: pFN21AB8351.seq Check: 6151 from: 1 to: 5527
pFN21AB8351 5527 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3337 278
Size: 53 83 186 936 1801 2468
Fragments arranged by size:
2468 1801 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2870 1937
Size: 933 4594
Acc65I G'GTAC_C
Cuts at: 2848 2848
Size: 5527
AclI AA'CG_TT
Cuts at: 1563 3654 4027 1563
Size: 2091 373 3063
Fragments arranged by size:
3063 2091 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3590 4974 5242 1632
Size: 1958 1384 268 1917
Fragments arranged by size:
1958 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5330
AflIII A'CryG_T
Cuts at: 1203 2721 4426 5403 1203
Size: 1518 1705 977 1327
Fragments arranged by size:
1705 1518 1327 977
AgeI A'CCGG_T
Cuts at: 4358 4358
Size: 5527
AhdI GACnn_n'nnGTC
Cuts at: 4256 4256
Size: 5527
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5527
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5527
AlwNI CAG_nnn'CTG
Cuts at: 2131 3270 4842 2131
Size: 1139 1572 2816
Fragments arranged by size:
2816 1572 1139
ApaLI G'TGCA_C
Cuts at: 3584 4740 3584
Size: 1156 4371
ApoI r'AATT_y
Cuts at: 1493 2836 3062 3214 5323 1493
Size: 1343 226 152 2109 1697
Fragments arranged by size:
2109 1697 1343 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4081 160
Size: 872 3049 1606
Fragments arranged by size:
3049 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5527
AvaI C'yCGr_G
Cuts at: 1870 1943 2354 2852 1870
Size: 73 411 498 4545
Fragments arranged by size:
4545 498 411 73
BamHI G'GATC_C
Cuts at: 2857 3224 2857
Size: 367 5160
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2153 2285 2326 2644 2848
Size: 325 217 627 366 132 41 318 204
Cuts at: 2848 4303 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 366 325 318 217 204
132 41
BanII G_rGCy'C
Cuts at: 1391 2055 2846 1391
Size: 664 791 4072
Fragments arranged by size:
4072 791 664
BbeI G_GCGC'C
Cuts at: 2289 2289
Size: 5527
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4556
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2378 2412 3718 3752 1903
Size: 34 441 34 1306 34 3678
Fragments arranged by size:
3678 1306 441 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3419 4629 3419
Size: 1210 4317
BclI T'GATC_A
Cuts at: 1548 2499 1548
Size: 951 4576
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2120 4138 136
Size: 107 122 71 631 605 448 2018 1525
Fragments arranged by size:
2018 1525 631 605 448 122 107 71
BglII A'GATC_T
Cuts at: 2358 5522 2358
Size: 3164 2363
BlpI GC'TnA_GC
Cuts at: 2944 2944
Size: 5527
Bme1580I G_kGCm'C
Cuts at: 1790 2156 2331 2607 2649 3588 4744 1790
Size: 366 175 276 42 939 1156 2573
Fragments arranged by size:
2573 1156 939 366 276 175 42
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5527
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3258 4216 442
Size: 416 53 83 399 24 1841 958 1753
Fragments arranged by size:
1841 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5527
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4187 1239
Size: 2948 2579
Bpu10I CC'TnA_GC
Cuts at: 2465 2465
Size: 5527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3652 4517 4815 3652
Size: 865 298 4364
Fragments arranged by size:
4364 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4190 882
Size: 617 2691 2219
Fragments arranged by size:
2691 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3318 493
Size: 711 2114 2702
Fragments arranged by size:
2702 2114 711
BsaBI GATnn'nnATC
Cuts at: 5521 5521
Size: 5527
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2112 2267 2286 3334
Size: 53 83 186 936 579 155 19 1048
Cuts at: 3334 3716 4351 275
Size: 382 635 1451
Fragments arranged by size:
1451 1048 936 635 579 382 186 155
83 53 19
BsaWI w'CCGG_w
Cuts at: 3960 4358 4632 4779 5190 3960
Size: 398 274 147 411 4297
Fragments arranged by size:
4297 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2133 2163 2438 2468 1178
Size: 30 165 30 730 30 275 30 4237
Fragments arranged by size:
4237 730 275 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2711 2834 1186
Size: 1525 123 3879
Fragments arranged by size:
3879 1525 123
BseYI C'CCAG_C
Cuts at: 2124 2553 2679 2769 4730 5226 2124
Size: 429 126 90 1961 496 2425
Fragments arranged by size:
2425 1961 496 429 126 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2305 1163
Size: 573 569 4385
Fragments arranged by size:
4385 573 569
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2392 2667 2977 3738 4766 1540
Size: 275 186 391 275 310 761 1028 2301
Fragments arranged by size:
2301 1028 761 391 310 275 275 186
BsiHKAI G_wGCw'C
Cuts at: 2055 3588 3673 4744 2055
Size: 1533 85 1071 2838
Fragments arranged by size:
2838 1533 1071 85
BsmI GAATG_Cn'
Cuts at: 3038 3131 3038
Size: 93 5434
BsmBI CGTCTCn'nnnn_
Cuts at: 2258 2258
Size: 5527
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2156 2331 2607 2649 2846 3588
Size: 399 265 101 175 276 42 197 742
Cuts at: 3588 3673 4744 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 276 265 197 175
101 85 42
BspEI T'CCGG_A
Cuts at: 5190 5190
Size: 5527
BspHI T'CATG_A
Cuts at: 3416 5146 3416
Size: 1730 3797
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2089 2882 844
Size: 1245 793 3489
Fragments arranged by size:
3489 1245 793
BsrBI CCG'CTC
Cuts at: 1394 1823 2469 2754 3414 1394
Size: 429 646 285 660 3507
Fragments arranged by size:
3507 660 646 429 285
BsrDI GCAATG_nn'
Cuts at: 65 1248 2394 4022 4196 65
Size: 1183 1146 1628 174 1396
Fragments arranged by size:
1628 1396 1183 1146 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2035 4171 4358 1700
Size: 110 225 2136 187 2869
Fragments arranged by size:
2869 2136 225 187 110
BsrGI T'GTAC_A
Cuts at: 96 2040 96
Size: 1944 3583
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5527
BssSI C'ACGA_G
Cuts at: 3587 4599 3587
Size: 1012 4515
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5527
BstBI TT'CG_AA
Cuts at: 4331 4331
Size: 5527
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5527
BstYI r'GATC_y
Cuts at: 1972 2358 2857 3224 3609 3626 5078 5164 5176
Size: 386 499 367 385 17 1452 86 12
Cuts at: 5176 5522 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 499 386 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2429 2429
Size: 5527
BtgI C'CryG_G
Cuts at: 513 1065 2004 2157 2271 2382 2538 2559 2649
Size: 552 939 153 114 111 156 21 90
Cuts at: 2649 513
Size: 3391
Fragments arranged by size:
3391 939 552 156 153 114 111 90
21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4788
Fragments arranged by size:
4788 683 56
BtsI GCAGTG_nn'
Cuts at: 2154 2317 3043 3837 3857 2154
Size: 163 726 794 20 3824
Fragments arranged by size:
3824 794 726 163 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5527
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5492
DraI TTT'AAA
Cuts at: 2831 2831
Size: 5527
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4534 809
Size: 507 3218 1802
Fragments arranged by size:
3218 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2196 2664 2974
Size: 54 1425 50 275 18 366 468 310
Cuts at: 2974 3863 5492 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 468 366 310 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2664 2974 1537
Size: 275 852 310 4090
Fragments arranged by size:
4090 852 310 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3457 1353
Size: 2104 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4114 4488 4634 387
Size: 1064 138 2525 374 146 1280
Fragments arranged by size:
2525 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2053 2053
Size: 5527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3590 4187 4974 5242 1239
Size: 393 1958 597 787 268 1524
Fragments arranged by size:
1958 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2432 2432
Size: 5527
EcoO109I rG'GnC_Cy
Cuts at: 2701 2701
Size: 5527
EcoRI G'AATT_C
Cuts at: 2836 3214 2836
Size: 378 5149
FspI TGC'GCA
Cuts at: 4033 4033
Size: 5527
HaeII r_GCGC'y
Cuts at: 1678 2289 4674 1678
Size: 611 2385 2531
Fragments arranged by size:
2531 2385 611
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2157 2189 4168 4200 4242
Size: 32 135 32 585 32 1979 32 42
Cuts at: 4242 4274 1373
Size: 32 2626
Fragments arranged by size:
2626 1979 585 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2871 3117 669
Size: 988 281 933 246 3079
Fragments arranged by size:
3079 988 933 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5527
HpaI GTT'AAC
Cuts at: 3117 3117
Size: 5527
KasI G'GCGC_C
Cuts at: 2285 2285
Size: 5527
KpnI G_GTAC'C
Cuts at: 2852 2852
Size: 5527
MfeI C'AATT_G
Cuts at: 3126 3126
Size: 5527
MluI A'CGCG_T
Cuts at: 5403 5403
Size: 5527
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4641 4825 1305
Size: 3336 184 2007
Fragments arranged by size:
3336 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2198 10
Size: 54 1425 343 366 3339
Fragments arranged by size:
3339 1425 366 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2543 2615 3485 3844 4003 518
Size: 648 1011 366 72 870 359 159 2042
Fragments arranged by size:
2042 1011 870 648 366 359 159 72
MspA1I CmG'CkG
Cuts at: 1606 2095 2107 2131 2301 2534 2624 2688 2943
Size: 489 12 24 170 233 90 64 255
Cuts at: 2943 3620 4768 5013 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 489 255 245 233 170
90 64 24 12
NarI GG'CG_CC
Cuts at: 2286 2286
Size: 5527
NcoI C'CATG_G
Cuts at: 513 1065 2004 2382 2538 513
Size: 552 939 378 156 3502
Fragments arranged by size:
3502 939 552 378 156
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5527
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5527
NotI GC'GGCC_GC
Cuts at: 2974 2974
Size: 5527
NspI r_CATG'y
Cuts at: 1132 1229 2725 2885 4430 1132
Size: 97 1496 160 1545 2229
Fragments arranged by size:
2229 1545 1496 160 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5527
PciI A'CATG_T
Cuts at: 2721 4426 2721
Size: 1705 3822
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2560 1108
Size: 1452 4075
PfoI T'CCnGG_A
Cuts at: 2418 2715 2418
Size: 297 5230
PmeI GTTT'AAAC
Cuts at: 2831 2831
Size: 5527
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3596 3628 5109 5141 3596
Size: 32 1481 32 3982
Fragments arranged by size:
3982 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2701 2701
Size: 5527
PshAI GACnn'nnGTC
Cuts at: 1613 2015 1613
Size: 402 5125
PsiI TTA'TAA
Cuts at: 3097 3097
Size: 5527
PstI C_TGCA'G
Cuts at: 830 2136 2811 2879 830
Size: 1306 675 68 3478
Fragments arranged by size:
3478 1306 675 68
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5527
PvuII CAG'CTG
Cuts at: 2107 2131 2534 2107
Size: 24 403 5100
Fragments arranged by size:
5100 403 24
SacI G_AGCT'C
Cuts at: 2055 2055
Size: 5527
SalI G'TCGA_C
Cuts at: 1936 2869 1936
Size: 933 4594
SbfI CC_TGCA'GG
Cuts at: 2879 2879
Size: 5527
ScaI AGT'ACT
Cuts at: 3775 5487 3775
Size: 1712 3815
SfcI C'TryA_G
Cuts at: 826 1045 2132 2629 2689 2807 2875 4010 4691
Size: 219 1087 497 60 118 68 1135 681
Cuts at: 4691 4882 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1087 681 497 219 191 118
68 60
SfoI GGC'GCC
Cuts at: 2287 2287
Size: 5527
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5527
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5527
SmaI CCC'GGG
Cuts at: 1872 2854 1872
Size: 982 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2354 3631 4532 4794 820
Size: 197 926 411 1277 901 262 1553
Fragments arranged by size:
1553 1277 926 901 411 262 197
SnaBI TAC'GTA
Cuts at: 493 3318 493
Size: 2825 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5527
SphI G_CATG'C
Cuts at: 1132 2885 1132
Size: 1753 3774
SspI AAT'ATT
Cuts at: 5 52 3451 5
Size: 47 3399 2081
Fragments arranged by size:
3399 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5527
StyI C'CwwG_G
Cuts at: 513 1065 2004 2382 2538 2966 513
Size: 552 939 378 156 428 3074
Fragments arranged by size:
3074 939 552 428 378 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3554 3571 3724 3909 4354 325
Size: 1255 1974 17 153 185 445 1498
Fragments arranged by size:
1974 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2040 3773 5485
Size: 274 80 33 51 1443 63 1733 1712
Cuts at: 5485 96
Size: 138
Fragments arranged by size:
1733 1712 1443 274 138 80 63 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2133 2165 2438 2470 5487 5519 2133
Size: 32 273 32 3017 32 2141
Fragments arranged by size:
3017 2141 273 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5527
XbaI T'CTAG_A
Cuts at: 2863 2863
Size: 5527
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2591 2591
Size: 5527
XhoI C'TCGA_G
Cuts at: 1943 2354 1943
Size: 411 5116
XmaI C'CCGG_G
Cuts at: 1870 2852 1870
Size: 982 4545
XmnI GAAnn'nnTTC
Cuts at: 3656 3656
Size: 5527
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3335 276
Size: 53 83 186 936 1801 2468
Fragments arranged by size:
2468 1801 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SrfI SwaI