Restriction map for
pFN21AB8344
(Circular) (Six-base) MAPSORT of: pFN21AB8344.seq Check: 6122 from: 1 to: 5608
pFN21AB8344 5608 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3418 278
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2951 1937
Size: 1014 4594
Acc65I G'GTAC_C
Cuts at: 2929 2929
Size: 5608
AclI AA'CG_TT
Cuts at: 1563 3735 4108 1563
Size: 2172 373 3063
Fragments arranged by size:
3063 2172 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2387 3671 5055 5323 1632
Size: 755 1284 1384 268 1917
Fragments arranged by size:
1917 1384 1284 755 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5411
AflIII A'CryG_T
Cuts at: 1203 2873 4507 5484 1203
Size: 1670 1634 977 1327
Fragments arranged by size:
1670 1634 1327 977
AgeI A'CCGG_T
Cuts at: 4439 4439
Size: 5608
AhdI GACnn_n'nnGTC
Cuts at: 4337 4337
Size: 5608
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5608
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2144 1184
Size: 960 4648
AlwNI CAG_nnn'CTG
Cuts at: 2141 3351 4923 2141
Size: 1210 1572 2826
Fragments arranged by size:
2826 1572 1210
ApaI G_GGCC'C
Cuts at: 2472 2472
Size: 5608
ApaLI G'TGCA_C
Cuts at: 3665 4821 3665
Size: 1156 4452
ApoI r'AATT_y
Cuts at: 1493 2813 2917 3143 3295 5404 1493
Size: 1320 104 226 152 2109 1697
Fragments arranged by size:
2109 1697 1320 226 152 104
AseI AT'TA_AT
Cuts at: 160 1032 4162 160
Size: 872 3130 1606
Fragments arranged by size:
3130 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
AvaI C'yCGr_G
Cuts at: 1870 1943 2933 1870
Size: 73 990 4545
Fragments arranged by size:
4545 990 73
BamHI G'GATC_C
Cuts at: 2938 3305 2938
Size: 367 5241
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2202 2672 2929 4384 618
Size: 325 217 627 415 470 257 1455 1842
Fragments arranged by size:
1842 1455 627 470 415 325 257 217
BanII G_rGCy'C
Cuts at: 1391 2472 2663 2927 1391
Size: 1081 191 264 4072
Fragments arranged by size:
4072 1081 264 191
BbeI G_GCGC'C
Cuts at: 2206 2676 2206
Size: 470 5138
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4637
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3799 3833 1903
Size: 34 1862 34 3678
Fragments arranged by size:
3678 1862 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3500 4710 3500
Size: 1210 4398
BclI T'GATC_A
Cuts at: 1548 2190 2265 1548
Size: 642 75 4891
Fragments arranged by size:
4891 642 75
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4219 136
Size: 107 122 71 631 605 2547 1525
Fragments arranged by size:
2547 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2493 5603 2493
Size: 3110 2498
BlpI GC'TnA_GC
Cuts at: 2142 3025 2142
Size: 883 4725
Bme1580I G_kGCm'C
Cuts at: 1790 2113 2472 3669 4825 1790
Size: 323 359 1197 1156 2573
Fragments arranged by size:
2573 1197 1156 359 323
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5608
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3339 4297 442
Size: 416 53 83 399 24 1922 958 1753
Fragments arranged by size:
1922 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5608
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2321 2482 2792 4268 1239
Size: 1082 161 310 1476 2579
Fragments arranged by size:
2579 1476 1082 310 161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3733 4598 4896 3733
Size: 865 298 4445
Fragments arranged by size:
4445 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4271 882
Size: 617 2772 2219
Fragments arranged by size:
2772 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2874 3399 493
Size: 711 1670 525 2702
Fragments arranged by size:
2702 1670 711 525
BsaBI GATnn'nnATC
Cuts at: 5602 5602
Size: 5608
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2203 2673 3415 3797
Size: 53 83 186 936 670 470 742 382
Cuts at: 3797 4432 275
Size: 635 1451
Fragments arranged by size:
1451 936 742 670 635 470 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2424 4041 4439 4713 4860 5271 2424
Size: 1617 398 274 147 411 2761
Fragments arranged by size:
2761 1617 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2228 2258 1178
Size: 30 165 30 825 30 4528
Fragments arranged by size:
4528 825 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2252 2324 2327 1186
Size: 1066 72 3 4467
Fragments arranged by size:
4467 1066 72 3
BseYI C'CCAG_C
Cuts at: 2029 2113 2632 2767 4811 5307 2029
Size: 84 519 135 2044 496 2330
Fragments arranged by size:
2330 2044 519 496 135 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5035
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3058 3819 4847 1540
Size: 275 186 1057 761 1028 2301
Fragments arranged by size:
2301 1057 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2663 3669 3754 4825 2663
Size: 1006 85 1071 3446
Fragments arranged by size:
3446 1071 1006 85
BsmI GAATG_Cn'
Cuts at: 2577 2701 3119 3212 2577
Size: 124 418 93 4973
Fragments arranged by size:
4973 418 124 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2113 2472 2663 2927 3669 3754 4825
Size: 399 323 359 191 264 742 85 1071
Cuts at: 4825 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 359 323 264 191
85
BspEI T'CCGG_A
Cuts at: 5271 5271
Size: 5608
BspHI T'CATG_A
Cuts at: 3497 5227 3497
Size: 1730 3878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2280 2473 2963 844
Size: 1436 193 490 3489
Fragments arranged by size:
3489 1436 490 193
BsrBI CCG'CTC
Cuts at: 1394 1823 3495 1394
Size: 429 1672 3507
Fragments arranged by size:
3507 1672 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4103 4277 65
Size: 1183 2855 174 1396
Fragments arranged by size:
2855 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4252 4439 1700
Size: 110 2442 187 2869
Fragments arranged by size:
2869 2442 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5608
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5608
BssSI C'ACGA_G
Cuts at: 2084 3668 4680 2084
Size: 1584 1012 3012
Fragments arranged by size:
3012 1584 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2613 794
Size: 1819 3789
BstBI TT'CG_AA
Cuts at: 4412 4412
Size: 5608
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5608
BstXI CCAn_nnnn'nTGG
Cuts at: 2816 2816
Size: 5608
BstYI r'GATC_y
Cuts at: 1972 2493 2938 3305 3690 3707 5159 5245 5257
Size: 521 445 367 385 17 1452 86 12
Cuts at: 5257 5603 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 521 445 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5056
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4869
Fragments arranged by size:
4869 683 56
BtsI GCAGTG_nn'
Cuts at: 2068 2249 3124 3918 3938 2068
Size: 181 875 794 20 3738
Fragments arranged by size:
3738 875 794 181 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5608
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5573
DraI TTT'AAA
Cuts at: 2912 2912
Size: 5608
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2290 4615 809
Size: 507 974 2325 1802
Fragments arranged by size:
2325 1802 974 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3055 3944 5573
Size: 54 1425 50 275 18 1225 889 1629
Cuts at: 5573 8
Size: 43
Fragments arranged by size:
1629 1425 1225 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3055 1537
Size: 275 1243 4090
Fragments arranged by size:
4090 1243 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2301 2400 2436 3538 1353
Size: 948 99 36 1102 3423
Fragments arranged by size:
3423 1102 948 99 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4195 4569 4715 387
Size: 1064 138 2606 374 146 1280
Fragments arranged by size:
2606 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2661 2661
Size: 5608
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2321 2387 2482 2792 3671 4268 5055
Size: 393 689 66 95 310 879 597 787
Cuts at: 5055 5323 1239
Size: 268 1524
Fragments arranged by size:
1524 879 787 689 597 393 310 268
95 66
EcoO109I rG'GnC_Cy
Cuts at: 2292 2468 2469 2292
Size: 176 1 5431
Fragments arranged by size:
5431 176 1
EcoRI G'AATT_C
Cuts at: 2917 3295 2917
Size: 378 5230
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2857 2857
Size: 5608
FspI TGC'GCA
Cuts at: 4114 4114
Size: 5608
HaeII r_GCGC'y
Cuts at: 1678 2206 2676 4755 1678
Size: 528 470 2079 2531
Fragments arranged by size:
2531 2079 528 470
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4249 4281 4323 4355 1373
Size: 32 135 32 2677 32 42 32 2626
Fragments arranged by size:
2677 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2170 2952 3198 669
Size: 988 281 232 782 246 3079
Fragments arranged by size:
3079 988 782 281 246 232
HindIII A'AGCT_T
Cuts at: 748 2799 748
Size: 2051 3557
HpaI GTT'AAC
Cuts at: 2170 3198 2170
Size: 1028 4580
KasI G'GCGC_C
Cuts at: 2202 2672 2202
Size: 470 5138
KpnI G_GTAC'C
Cuts at: 2933 2933
Size: 5608
MfeI C'AATT_G
Cuts at: 3207 3207
Size: 5608
MluI A'CGCG_T
Cuts at: 5484 5484
Size: 5608
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2199 4722 4906 1305
Size: 894 2523 184 2007
Fragments arranged by size:
2523 2007 894 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3786
Fragments arranged by size:
3786 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2301 2565 3566 3925 4084 518
Size: 648 1135 264 1001 359 159 2042
Fragments arranged by size:
2042 1135 1001 648 359 264 159
MspA1I CmG'CkG
Cuts at: 1606 2636 2767 3024 3701 4849 5094 1606
Size: 1030 131 257 677 1148 245 2120
Fragments arranged by size:
2120 1148 1030 677 257 245 131
NarI GG'CG_CC
Cuts at: 2203 2673 2203
Size: 470 5138
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5056
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5608
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5608
NotI GC'GGCC_GC
Cuts at: 3055 3055
Size: 5608
NspI r_CATG'y
Cuts at: 1132 1229 2966 4511 1132
Size: 97 1737 1545 2229
Fragments arranged by size:
2229 1737 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5608
PciI A'CATG_T
Cuts at: 4507 4507
Size: 5608
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5608
PfoI T'CCnGG_A
Cuts at: 2337 2808 2337
Size: 471 5137
PmeI GTTT'AAAC
Cuts at: 2912 2912
Size: 5608
PmlI CAC'GTG
Cuts at: 2874 2874
Size: 5608
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3677 3709 5190 5222 3677
Size: 32 1481 32 4063
Fragments arranged by size:
4063 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2292 2292
Size: 5608
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5608
PsiI TTA'TAA
Cuts at: 3178 3178
Size: 5608
PspOMI G'GGCC_C
Cuts at: 2468 2468
Size: 5608
PstI C_TGCA'G
Cuts at: 830 2046 2137 2418 2960 830
Size: 1216 91 281 542 3478
Fragments arranged by size:
3478 1216 542 281 91
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5608
PvuII CAG'CTG
Cuts at: 2636 2767 2636
Size: 131 5477
SacI G_AGCT'C
Cuts at: 2663 2663
Size: 5608
SalI G'TCGA_C
Cuts at: 1936 2950 1936
Size: 1014 4594
SbfI CC_TGCA'GG
Cuts at: 2960 2960
Size: 5608
ScaI AGT'ACT
Cuts at: 2048 3856 5568 2048
Size: 1808 1712 2088
Fragments arranged by size:
2088 1808 1712
SfcI C'TryA_G
Cuts at: 826 1045 2042 2053 2133 2414 2956 4091 4772
Size: 219 997 11 80 281 542 1135 681
Cuts at: 4772 4963 826
Size: 191 1471
Fragments arranged by size:
1471 1135 997 681 542 281 219 191
80 11
SfoI GGC'GCC
Cuts at: 2204 2674 2204
Size: 470 5138
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5608
SmaI CCC'GGG
Cuts at: 1872 2935 1872
Size: 1063 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3712 4613 4875 820
Size: 197 926 1769 901 262 1553
Fragments arranged by size:
1769 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3399 493
Size: 2906 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5608
SphI G_CATG'C
Cuts at: 1132 2966 1132
Size: 1834 3774
SspI AAT'ATT
Cuts at: 5 52 3532 5
Size: 47 3480 2081
Fragments arranged by size:
3480 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5608
StyI C'CwwG_G
Cuts at: 513 1065 3047 513
Size: 552 1982 3074
Fragments arranged by size:
3074 1982 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2112 2603 3635 3652 3805 3990 4435
Size: 1255 532 491 1032 17 153 185 445
Cuts at: 4435 325
Size: 1498
Fragments arranged by size:
1498 1255 1032 532 491 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2046 3854 5566
Size: 274 80 33 51 1443 69 1808 1712
Cuts at: 5566 96
Size: 138
Fragments arranged by size:
1808 1712 1443 274 138 80 69 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2228 2260 5568 5600 2228
Size: 32 3308 32 2236
Fragments arranged by size:
3308 2236 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5608
XbaI T'CTAG_A
Cuts at: 2944 2944
Size: 5608
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5608
XmaI C'CCGG_G
Cuts at: 1870 2933 1870
Size: 1063 4545
XmnI GAAnn'nnTTC
Cuts at: 2409 2802 3737 2409
Size: 393 935 4280
Fragments arranged by size:
4280 935 393
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3416 276
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SrfI SwaI XcmI