Restriction map for
pFN21AB8336
(Circular) (Six-base) MAPSORT of: pFN21AB8336.seq Check: 4951 from: 1 to: 6178
pFN21AB8336 6178 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3988 278
Size: 53 83 186 936 2452 2468
Fragments arranged by size:
2468 2452 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3521 1937
Size: 1584 4594
Acc65I G'GTAC_C
Cuts at: 3499 3499
Size: 6178
AclI AA'CG_TT
Cuts at: 1563 4305 4678 1563
Size: 2742 373 3063
Fragments arranged by size:
3063 2742 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2610 2718 2902 2986 3070 3154 3238 3406
Size: 978 108 184 84 84 84 84 168
Cuts at: 3406 4241 5625 5893 1632
Size: 835 1384 268 1917
Fragments arranged by size:
1917 1384 978 835 268 184 168 108
84 84 84 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5981
AflIII A'CryG_T
Cuts at: 1203 3153 3237 5077 6054 1203
Size: 1950 84 1840 977 1327
Fragments arranged by size:
1950 1840 1327 977 84
AgeI A'CCGG_T
Cuts at: 5009 5009
Size: 6178
AhdI GACnn_n'nnGTC
Cuts at: 4907 4907
Size: 6178
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6178
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6178
AlwNI CAG_nnn'CTG
Cuts at: 2236 2269 2296 2395 3261 3408 3921 5493 2236
Size: 33 27 99 866 147 513 1572 2921
Fragments arranged by size:
2921 1572 866 513 147 99 33 27
ApaLI G'TGCA_C
Cuts at: 3122 4235 5391 3122
Size: 1113 1156 3909
Fragments arranged by size:
3909 1156 1113
ApoI r'AATT_y
Cuts at: 1493 2744 3487 3713 3865 5974 1493
Size: 1251 743 226 152 2109 1697
Fragments arranged by size:
2109 1697 1251 743 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4732 160
Size: 872 3700 1606
Fragments arranged by size:
3700 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6178
AvaI C'yCGr_G
Cuts at: 1870 1943 2243 2680 2852 3503 1870
Size: 73 300 437 172 651 4545
Fragments arranged by size:
4545 651 437 300 172 73
BamHI G'GATC_C
Cuts at: 2413 3372 3456 3508 3875 2413
Size: 959 84 52 367 4716
Fragments arranged by size:
4716 959 367 84 52
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2675 3499 4954 618
Size: 325 217 627 888 824 1455 1842
Fragments arranged by size:
1842 1455 888 824 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2033 2338 2430 3350 3497 1391
Size: 642 305 92 920 147 4072
Fragments arranged by size:
4072 920 642 305 147 92
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2745 2873 2888 928
Size: 971 846 128 15 4218
Fragments arranged by size:
4218 971 846 128 15
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2205 2239 2917 2951 4369 4403 1903
Size: 34 268 34 678 34 1418 34 3678
Fragments arranged by size:
3678 1418 678 268 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4070 5280 4070
Size: 1210 4968
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6178
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4789 136
Size: 107 122 71 631 605 3117 1525
Fragments arranged by size:
3117 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2580 6173 2580
Size: 3593 2585
BlpI GC'TnA_GC
Cuts at: 3265 3433 3595 3265
Size: 168 162 5848
Fragments arranged by size:
5848 168 162
Bme1580I G_kGCm'C
Cuts at: 1790 3126 4239 5395 1790
Size: 1336 1113 1156 2573
Fragments arranged by size:
2573 1336 1156 1113
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6178
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2027 3909 4867
Size: 416 53 83 399 24 610 1882 958
Cuts at: 4867 442
Size: 1753
Fragments arranged by size:
1882 1753 958 610 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6178
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2269 2569 2269
Size: 300 5878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2291 2306 3066 3318 4838 1239
Size: 1052 15 760 252 1520 2579
Fragments arranged by size:
2579 1520 1052 760 252 15
Bpu10I CC'TnA_GC
Cuts at: 2027 2027
Size: 6178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4303 5168 5466 4303
Size: 865 298 5015
Fragments arranged by size:
5015 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2483 4841 882
Size: 617 984 2358 2219
Fragments arranged by size:
2358 2219 984 617
BsaAI yAC'GTr
Cuts at: 493 1204 3969 493
Size: 711 2765 2702
Fragments arranged by size:
2765 2702 711
BsaBI GATnn'nnATC
Cuts at: 3461 6172 3461
Size: 2711 3467
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3985 4367 5002 275
Size: 53 83 186 936 2452 382 635 1451
Fragments arranged by size:
2452 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4611 5009 5283 5430 5841 4611
Size: 398 274 147 411 4948
Fragments arranged by size:
4948 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2699 2729 2761 2791 1178
Size: 30 165 30 1296 30 32 30 4565
Fragments arranged by size:
4565 1296 165 32 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 2192 3340 1186
Size: 904 102 1148 4024
Fragments arranged by size:
4024 1148 904 102
BseYI C'CCAG_C
Cuts at: 2940 3108 5381 5877 2940
Size: 168 2273 496 3241
Fragments arranged by size:
3241 2273 496 168
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2130 3421 1163
Size: 573 394 1291 3920
Fragments arranged by size:
3920 1291 573 394
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3628 4389 5417 1540
Size: 275 186 1627 761 1028 2301
Fragments arranged by size:
2301 1627 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2930 3126 3350 3366 4239 4324 5395 2930
Size: 196 224 16 873 85 1071 3713
Fragments arranged by size:
3713 1071 873 224 196 85 16
BsmI GAATG_Cn'
Cuts at: 3689 3782 3689
Size: 93 6085
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2338 2430 2930 3126 3350 3366
Size: 399 243 305 92 500 196 224 16
Cuts at: 3366 3497 4239 4324 5395 1391
Size: 131 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 500 399 305 243 224
196 131 92 85 16
BspEI T'CCGG_A
Cuts at: 5841 5841
Size: 6178
BspHI T'CATG_A
Cuts at: 4067 5797 4067
Size: 1730 4448
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3533 844
Size: 2689 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2352 3136 4065 1394
Size: 429 529 784 929 3507
Fragments arranged by size:
3507 929 784 529 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2127 4673 4847 65
Size: 1183 879 2546 174 1396
Fragments arranged by size:
2546 1396 1183 879 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4822 5009 1700
Size: 110 3012 187 2869
Fragments arranged by size:
3012 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6178
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6178
BssSI C'ACGA_G
Cuts at: 2195 4238 5250 2195
Size: 2043 1012 3123
Fragments arranged by size:
3123 2043 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6178
BstBI TT'CG_AA
Cuts at: 4982 4982
Size: 6178
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6178
BstXI CCAn_nnnn'nTGG
Cuts at: 2040 2040
Size: 6178
BstYI r'GATC_y
Cuts at: 1972 2316 2380 2413 2580 2607 3372 3456 3508
Size: 344 64 33 167 27 765 84 52
Cuts at: 3508 3875 4260 4277 5729 5815 5827 6173 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 765 385 367 346 344 167
86 84 64 52 33 27 17 12
Bsu36I CC'TnA_GG
Cuts at: 2544 2544
Size: 6178
BtgI C'CryG_G
Cuts at: 513 1065 2004 2039 513
Size: 552 939 35 4652
Fragments arranged by size:
4652 939 552 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5439
Fragments arranged by size:
5439 683 56
BtsI GCAGTG_nn'
Cuts at: 3694 4488 4508 3694
Size: 794 20 5364
Fragments arranged by size:
5364 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6178
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6143
DraI TTT'AAA
Cuts at: 3482 3482
Size: 6178
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5185 809
Size: 507 3869 1802
Fragments arranged by size:
3869 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2662 2702
Size: 54 1425 50 275 18 177 655 40
Cuts at: 2702 3244 3625 4514 6143 8
Size: 542 381 889 1629 43
Fragments arranged by size:
1629 1425 889 655 542 381 275 177
54 50 43 40 18
EagI C'GGCC_G
Cuts at: 1537 1812 3625 1537
Size: 275 1813 4090
Fragments arranged by size:
4090 1813 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2040 2076 2792 4108 1353
Size: 687 36 716 1316 3423
Fragments arranged by size:
3423 1316 716 687 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2121 4765 5139 5285 387
Size: 1064 138 532 2644 374 146 1280
Fragments arranged by size:
2644 1280 1064 532 374 146 138
EcoICRI GAG'CTC
Cuts at: 3348 3348
Size: 6178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2291 2306 2610 2718 2902 2986 3066
Size: 393 659 15 304 108 184 84 80
Cuts at: 3066 3070 3154 3238 3318 3406 4241 4838 5625
Size: 4 84 84 80 88 835 597 787
Cuts at: 5625 5893 1239
Size: 268 1524
Fragments arranged by size:
1524 835 787 659 597 393 304 268
184 108 88 84 84 84 80 80
15 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2099 2099
Size: 6178
EcoO109I rG'GnC_Cy
Cuts at: 2200 2630 2802 2200
Size: 430 172 5576
Fragments arranged by size:
5576 430 172
EcoRI G'AATT_C
Cuts at: 3487 3865 3487
Size: 378 5800
FspI TGC'GCA
Cuts at: 4684 4684
Size: 6178
HaeII r_GCGC'y
Cuts at: 1678 2852 5325 1678
Size: 1174 2473 2531
Fragments arranged by size:
2531 2473 1174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2168 2200 2761 2793 2860
Size: 32 135 32 596 32 561 32 67
Cuts at: 2860 2892 3265 3297 4819 4851 4893 4925 1373
Size: 32 373 32 1522 32 42 32 2626
Fragments arranged by size:
2626 1522 596 561 373 135 67 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3522 3768 669
Size: 988 281 1584 246 3079
Fragments arranged by size:
3079 1584 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6178
HpaI GTT'AAC
Cuts at: 3768 3768
Size: 6178
KpnI G_GTAC'C
Cuts at: 3503 3503
Size: 6178
MfeI C'AATT_G
Cuts at: 3777 3777
Size: 6178
MluI A'CGCG_T
Cuts at: 3153 3237 6054 3153
Size: 84 2817 3277
Fragments arranged by size:
3277 2817 84
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3301 5292 5476 1305
Size: 1996 1991 184 2007
Fragments arranged by size:
2007 1996 1991 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2704 10
Size: 54 1425 343 177 695 3484
Fragments arranged by size:
3484 1425 695 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3313 3467 4136 4495 4654 518
Size: 648 2147 154 669 359 159 2042
Fragments arranged by size:
2147 2042 669 648 359 159 154
MspA1I CmG'CkG
Cuts at: 1606 2366 3118 3594 4271 5419 5664 1606
Size: 760 752 476 677 1148 245 2120
Fragments arranged by size:
2120 1148 760 752 677 476 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4687
Fragments arranged by size:
4687 939 552
NdeI CA'TA_TG
Cuts at: 387 2890 387
Size: 2503 3675
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6178
NotI GC'GGCC_GC
Cuts at: 3625 3625
Size: 6178
NruI TCG'CGA
Cuts at: 2817 2817
Size: 6178
NspI r_CATG'y
Cuts at: 1132 1229 2538 3536 5081 1132
Size: 97 1309 998 1545 2229
Fragments arranged by size:
2229 1545 1309 998 97
PasI CC'CwG_GG
Cuts at: 1348 2101 1348
Size: 753 5425
PciI A'CATG_T
Cuts at: 5077 5077
Size: 6178
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6178
PfoI T'CCnGG_A
Cuts at: 2416 2416
Size: 6178
PmeI GTTT'AAAC
Cuts at: 3482 3482
Size: 6178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2674 2706 4247 4279 5760 5792 2674
Size: 32 1541 32 1481 32 3060
Fragments arranged by size:
3060 1541 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2802 2802
Size: 6178
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6178
PsiI TTA'TAA
Cuts at: 3396 3748 3396
Size: 352 5826
PspXI vC'TCGA_Gb
Cuts at: 2680 2852 2680
Size: 172 6006
PstI C_TGCA'G
Cuts at: 830 2241 2900 3530 830
Size: 1411 659 630 3478
Fragments arranged by size:
3478 1411 659 630
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6178
SacI G_AGCT'C
Cuts at: 3350 3350
Size: 6178
SalI G'TCGA_C
Cuts at: 1936 3520 1936
Size: 1584 4594
SbfI CC_TGCA'GG
Cuts at: 3530 3530
Size: 6178
ScaI AGT'ACT
Cuts at: 4426 6138 4426
Size: 1712 4466
SexAI A'CCwGG_T
Cuts at: 2946 2946
Size: 6178
SfcI C'TryA_G
Cuts at: 826 1045 2237 2896 3526 4661 5342 5533 826
Size: 219 1192 659 630 1135 681 191 1471
Fragments arranged by size:
1471 1192 1135 681 659 630 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6178
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6178
SmaI CCC'GGG
Cuts at: 1872 3505 1872
Size: 1633 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2680 2852 4282 5183 5445 820
Size: 197 926 737 172 1430 901 262 1553
Fragments arranged by size:
1553 1430 926 901 737 262 197 172
SnaBI TAC'GTA
Cuts at: 493 3969 493
Size: 3476 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6178
SphI G_CATG'C
Cuts at: 1132 3536 1132
Size: 2404 3774
SspI AAT'ATT
Cuts at: 5 52 4102 5
Size: 47 4050 2081
Fragments arranged by size:
4050 2081 47
StuI AGG'CCT
Cuts at: 1517 2999 3335 3419 1517
Size: 1482 336 84 4276
Fragments arranged by size:
4276 1482 336 84
StyI C'CwwG_G
Cuts at: 513 1065 2004 2256 2512 3617 513
Size: 552 939 252 256 1105 3074
Fragments arranged by size:
3074 1105 939 552 256 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2288 2317 2615 3029 3122 4205 4222
Size: 1255 708 29 298 414 93 1083 17
Cuts at: 4222 4375 4560 5005 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1083 708 445 414 298 185
153 93 29 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4424 6136 96
Size: 274 80 33 51 1443 2447 1712 138
Fragments arranged by size:
2447 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6138 6170 6138
Size: 32 6146
Tth111I GACn'n_nGTC
Cuts at: 1316 2590 1316
Size: 1274 4904
XbaI T'CTAG_A
Cuts at: 3514 3514
Size: 6178
XhoI C'TCGA_G
Cuts at: 1943 2680 2852 1943
Size: 737 172 5269
Fragments arranged by size:
5269 737 172
XmaI C'CCGG_G
Cuts at: 1870 3503 1870
Size: 1633 4545
XmnI GAAnn'nnTTC
Cuts at: 2831 2915 2999 3167 3419 4307 2831
Size: 84 84 168 252 888 4702
Fragments arranged by size:
4702 888 252 168 84 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3986 276
Size: 53 83 186 936 2452 2468
Fragments arranged by size:
2468 2452 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NruI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI SacI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI
PmlI PspOMI PsrI PvuII RsrII SacII SanDI SapI
SfiI SfoI SrfI SwaI XcmI