Restriction map for
pFN21AB8328
(Circular) (Six-base) MAPSORT of: pFN21AB8328.seq Check: 2690 from: 1 to: 5224
pFN21AB8328 5224 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2214 3034 278
Size: 53 83 186 936 678 820 2468
Fragments arranged by size:
2468 936 820 678 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2567 1937
Size: 630 4594
Acc65I G'GTAC_C
Cuts at: 2545 2545
Size: 5224
AclI AA'CG_TT
Cuts at: 1563 3351 3724 1563
Size: 1788 373 3063
Fragments arranged by size:
3063 1788 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2482 3287 4671 4939 1632
Size: 850 805 1384 268 1917
Fragments arranged by size:
1917 1384 850 805 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5027
AflIII A'CryG_T
Cuts at: 1203 4123 5100 1203
Size: 2920 977 1327
Fragments arranged by size:
2920 1327 977
AgeI A'CCGG_T
Cuts at: 4055 4055
Size: 5224
AhdI GACnn_n'nnGTC
Cuts at: 2332 3953 2332
Size: 1621 3603
AleI CACnn'nnGTG
Cuts at: 1166 2453 1166
Size: 1287 3937
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5224
AlwNI CAG_nnn'CTG
Cuts at: 2967 4539 2967
Size: 1572 3652
ApaI G_GGCC'C
Cuts at: 2439 2439
Size: 5224
ApaLI G'TGCA_C
Cuts at: 3281 4437 3281
Size: 1156 4068
ApoI r'AATT_y
Cuts at: 1493 2533 2759 2911 5020 1493
Size: 1040 226 152 2109 1697
Fragments arranged by size:
2109 1697 1040 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3778 160
Size: 872 2746 1606
Fragments arranged by size:
2746 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5224
AvaI C'yCGr_G
Cuts at: 1870 1943 2238 2549 1870
Size: 73 295 311 4545
Fragments arranged by size:
4545 311 295 73
BamHI G'GATC_C
Cuts at: 2554 2921 2554
Size: 367 4857
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2027 2303 2545 4000 618
Size: 325 217 627 240 276 242 1455 1842
Fragments arranged by size:
1842 1455 627 325 276 242 240 217
BanII G_rGCy'C
Cuts at: 1391 2091 2439 2543 1391
Size: 700 348 104 4072
Fragments arranged by size:
4072 700 348 104
BbeI G_GCGC'C
Cuts at: 2031 2031
Size: 5224
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2140 928
Size: 971 241 4012
Fragments arranged by size:
4012 971 241
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3415 3449 1903
Size: 34 1478 34 3678
Fragments arranged by size:
3678 1478 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3116 4326 3116
Size: 1210 4014
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5224
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3835 136
Size: 107 122 71 631 605 337 1826 1525
Fragments arranged by size:
1826 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2160 5219 2160
Size: 3059 2165
BlpI GC'TnA_GC
Cuts at: 2068 2641 2068
Size: 573 4651
Bme1580I G_kGCm'C
Cuts at: 1790 2426 2439 3285 4441 1790
Size: 636 13 846 1156 2573
Fragments arranged by size:
2573 1156 846 636 13
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5224
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2955 3913 442
Size: 416 53 83 399 24 1538 958 1753
Fragments arranged by size:
1753 1538 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5224
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2105 2293 3884 1239
Size: 866 188 1591 2579
Fragments arranged by size:
2579 1591 866 188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3349 4214 4512 3349
Size: 865 298 4061
Fragments arranged by size:
4061 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2135 3887 882
Size: 617 636 1752 2219
Fragments arranged by size:
2219 1752 636 617
BsaAI yAC'GTr
Cuts at: 493 1204 3015 493
Size: 711 1811 2702
Fragments arranged by size:
2702 1811 711
BsaBI GATnn'nnATC
Cuts at: 5218 5218
Size: 5224
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2028 2211 3031 3413
Size: 53 83 186 936 495 183 820 382
Cuts at: 3413 4048 275
Size: 635 1451
Fragments arranged by size:
1451 936 820 635 495 382 186 183
83 53
BsaWI w'CCGG_w
Cuts at: 3657 4055 4329 4476 4887 3657
Size: 398 274 147 411 3994
Fragments arranged by size:
3994 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4999
Fragments arranged by size:
4999 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2336 2345 1186
Size: 1150 9 4065
Fragments arranged by size:
4065 1150 9
BseYI C'CCAG_C
Cuts at: 4427 4923 4427
Size: 496 4728
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4651
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2472 2674 3435 4463 1540
Size: 275 186 471 202 761 1028 2301
Fragments arranged by size:
2301 1028 761 471 275 202 186
BsiHKAI G_wGCw'C
Cuts at: 3285 3370 4441 3285
Size: 85 1071 4068
Fragments arranged by size:
4068 1071 85
BsmI GAATG_Cn'
Cuts at: 2735 2828 2735
Size: 93 5131
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2426 2439 2543 3285 3370 4441
Size: 399 301 335 13 104 742 85 1071
Cuts at: 4441 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 335 301 104 85
13
BspEI T'CCGG_A
Cuts at: 4887 4887
Size: 5224
BspHI T'CATG_A
Cuts at: 3113 4843 3113
Size: 1730 3494
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2579 844
Size: 1735 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3111 1394
Size: 429 1288 3507
Fragments arranged by size:
3507 1288 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3719 3893 65
Size: 1183 2471 174 1396
Fragments arranged by size:
2471 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3868 4055 1700
Size: 110 2058 187 2869
Fragments arranged by size:
2869 2058 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5224
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5224
BssSI C'ACGA_G
Cuts at: 3284 4296 3284
Size: 1012 4212
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5224
BstBI TT'CG_AA
Cuts at: 4028 4028
Size: 5224
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5224
BstYI r'GATC_y
Cuts at: 1972 2160 2554 2921 3306 3323 4775 4861 4873
Size: 188 394 367 385 17 1452 86 12
Cuts at: 4873 5219 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 394 385 367 346 188 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2348 2348
Size: 5224
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3733
Fragments arranged by size:
3733 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2027 487
Size: 683 56 801 3684
Fragments arranged by size:
3684 801 683 56
BtsI GCAGTG_nn'
Cuts at: 2740 3534 3554 2740
Size: 794 20 4410
Fragments arranged by size:
4410 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5224
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5189
DraI TTT'AAA
Cuts at: 2528 2528
Size: 5224
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4231 809
Size: 507 2915 1802
Fragments arranged by size:
2915 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2671 3560 5189
Size: 54 1425 50 275 18 841 889 1629
Cuts at: 5189 8
Size: 43
Fragments arranged by size:
1629 1425 889 841 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2671 1537
Size: 275 859 4090
Fragments arranged by size:
4090 859 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2501 3154 1353
Size: 1148 653 3423
Fragments arranged by size:
3423 1148 653
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3811 4185 4331 387
Size: 1064 138 2222 374 146 1280
Fragments arranged by size:
2222 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2293 2482 3287 3884 4671 4939
Size: 393 473 188 189 805 597 787 268
Cuts at: 4939 1239
Size: 1524
Fragments arranged by size:
1524 805 787 597 473 393 268 189
188
EcoNI CCTnn'n_nnAGG
Cuts at: 2351 2351
Size: 5224
EcoO109I rG'GnC_Cy
Cuts at: 2436 2436
Size: 5224
EcoRI G'AATT_C
Cuts at: 2533 2911 2533
Size: 378 4846
FspI TGC'GCA
Cuts at: 3730 3730
Size: 5224
HaeII r_GCGC'y
Cuts at: 1678 2031 2039 2295 4371 1678
Size: 353 8 256 2076 2531
Fragments arranged by size:
2531 2076 353 256 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2005 2037 2318 2350 3865
Size: 32 135 32 433 32 281 32 1515
Cuts at: 3865 3897 3939 3971 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1515 433 281 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2568 2814 669
Size: 988 281 630 246 3079
Fragments arranged by size:
3079 988 630 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5224
HpaI GTT'AAC
Cuts at: 2814 2814
Size: 5224
KasI G'GCGC_C
Cuts at: 2027 2027
Size: 5224
KpnI G_GTAC'C
Cuts at: 2549 2549
Size: 5224
MfeI C'AATT_G
Cuts at: 2823 2823
Size: 5224
MluI A'CGCG_T
Cuts at: 5100 5100
Size: 5224
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4338 4522 1305
Size: 3033 184 2007
Fragments arranged by size:
3033 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3402
Fragments arranged by size:
3402 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2453 3182 3541 3700 518
Size: 648 1287 729 359 159 2042
Fragments arranged by size:
2042 1287 729 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2064 2640 3317 4465 4710 1606
Size: 458 576 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 576 458 245
NarI GG'CG_CC
Cuts at: 2028 2028
Size: 5224
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3733
Fragments arranged by size:
3733 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5224
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5224
NotI GC'GGCC_GC
Cuts at: 2671 2671
Size: 5224
NspI r_CATG'y
Cuts at: 1132 1229 2175 2582 4127 1132
Size: 97 946 407 1545 2229
Fragments arranged by size:
2229 1545 946 407 97
PasI CC'CwG_GG
Cuts at: 1348 2439 1348
Size: 1091 4133
PciI A'CATG_T
Cuts at: 4123 4123
Size: 5224
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2194 2251 1108
Size: 1086 57 4081
Fragments arranged by size:
4081 1086 57
PfoI T'CCnGG_A
Cuts at: 2270 2270
Size: 5224
PmeI GTTT'AAAC
Cuts at: 2528 2528
Size: 5224
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3293 3325 4806 4838 3293
Size: 32 1481 32 3679
Fragments arranged by size:
3679 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5224
PsiI TTA'TAA
Cuts at: 2794 2794
Size: 5224
PspOMI G'GGCC_C
Cuts at: 2435 2435
Size: 5224
PspXI vC'TCGA_Gb
Cuts at: 2238 2238
Size: 5224
PstI C_TGCA'G
Cuts at: 830 2576 830
Size: 1746 3478
PvuI CG_AT'CG
Cuts at: 2001 2472 2001
Size: 471 4753
SalI G'TCGA_C
Cuts at: 1936 2566 1936
Size: 630 4594
SapI GCTCTTCn'nnn_
Cuts at: 2501 2501
Size: 5224
SbfI CC_TGCA'GG
Cuts at: 2576 2576
Size: 5224
ScaI AGT'ACT
Cuts at: 3472 5184 3472
Size: 1712 3512
SfcI C'TryA_G
Cuts at: 826 1045 2338 2572 3707 4388 4579 826
Size: 219 1293 234 1135 681 191 1471
Fragments arranged by size:
1471 1293 1135 681 234 219 191
SfoI GGC'GCC
Cuts at: 2029 2029
Size: 5224
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5224
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5224
SmaI CCC'GGG
Cuts at: 1872 2551 1872
Size: 679 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2238 3328 4229 4491 820
Size: 197 926 295 1090 901 262 1553
Fragments arranged by size:
1553 1090 926 901 295 262 197
SnaBI TAC'GTA
Cuts at: 493 3015 493
Size: 2522 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5224
SphI G_CATG'C
Cuts at: 1132 2582 1132
Size: 1450 3774
SspI AAT'ATT
Cuts at: 5 52 3148 5
Size: 47 3096 2081
Fragments arranged by size:
3096 2081 47
StuI AGG'CCT
Cuts at: 1517 2078 1517
Size: 561 4663
StyI C'CwwG_G
Cuts at: 513 1065 2004 2663 513
Size: 552 939 659 3074
Fragments arranged by size:
3074 939 659 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2431 3251 3268 3421 3606 4051 325
Size: 1255 851 820 17 153 185 445 1498
Fragments arranged by size:
1498 1255 851 820 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3470 5182 96
Size: 274 80 33 51 1443 1493 1712 138
Fragments arranged by size:
1712 1493 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5184 5216 5184
Size: 32 5192
Tth111I GACn'n_nGTC
Cuts at: 1316 2126 1316
Size: 810 4414
XbaI T'CTAG_A
Cuts at: 2560 2560
Size: 5224
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2455 2011
Size: 444 4780
XhoI C'TCGA_G
Cuts at: 1943 2238 1943
Size: 295 4929
XmaI C'CCGG_G
Cuts at: 1870 2549 1870
Size: 679 4545
XmnI GAAnn'nnTTC
Cuts at: 3353 3353
Size: 5224
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2212 3032 276
Size: 53 83 186 936 678 820 2468
Fragments arranged by size:
2468 936 820 678 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PspXI PstI PvuI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PpuMI PsrI PvuII RsrII SacI SacII
SanDI SexAI SfiI SrfI SwaI