Restriction map for
pFN21AB8327
(Circular) (Six-base) MAPSORT of: pFN21AB8327.seq Check: 1050 from: 1 to: 5467
pFN21AB8327 5467 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3277 278
Size: 53 83 186 936 1741 2468
Fragments arranged by size:
2468 1741 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2810 1937
Size: 873 4594
Acc65I G'GTAC_C
Cuts at: 2788 2788
Size: 5467
AclI AA'CG_TT
Cuts at: 1563 3594 3967 1563
Size: 2031 373 3063
Fragments arranged by size:
3063 2031 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3530 4914 5182 1632
Size: 1898 1384 268 1917
Fragments arranged by size:
1917 1898 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5270
AflIII A'CryG_T
Cuts at: 1203 4366 5343 1203
Size: 3163 977 1327
Fragments arranged by size:
3163 1327 977
AgeI A'CCGG_T
Cuts at: 4298 4298
Size: 5467
AhdI GACnn_n'nnGTC
Cuts at: 2134 4196 2134
Size: 2062 3405
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5467
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2529 1184
Size: 1345 4122
AlwNI CAG_nnn'CTG
Cuts at: 3210 4782 3210
Size: 1572 3895
ApaI G_GGCC'C
Cuts at: 2329 2329
Size: 5467
ApaLI G'TGCA_C
Cuts at: 3524 4680 3524
Size: 1156 4311
ApoI r'AATT_y
Cuts at: 1493 2776 3002 3154 5263 1493
Size: 1283 226 152 2109 1697
Fragments arranged by size:
2109 1697 1283 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4021 160
Size: 872 2989 1606
Fragments arranged by size:
2989 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5467
AvaI C'yCGr_G
Cuts at: 1870 1943 2139 2181 2266 2792 1870
Size: 73 196 42 85 526 4545
Fragments arranged by size:
4545 526 196 85 73 42
BamHI G'GATC_C
Cuts at: 2797 3164 2797
Size: 367 5100
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2788 4243 618
Size: 325 217 627 1001 1455 1842
Fragments arranged by size:
1842 1455 1001 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2068 2329 2786 1391
Size: 677 261 457 4072
Fragments arranged by size:
4072 677 457 261
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4496
BbvCI CC'TCA_GC
Cuts at: 2654 2706 2654
Size: 52 5415
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3658 3692 1903
Size: 34 1721 34 3678
Fragments arranged by size:
3678 1721 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3359 4569 3359
Size: 1210 4257
BclI T'GATC_A
Cuts at: 1548 2196 1548
Size: 648 4819
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2052 4078 136
Size: 107 122 71 631 605 380 2026 1525
Fragments arranged by size:
2026 1525 631 605 380 122 107 71
BglII A'GATC_T
Cuts at: 2547 5462 2547
Size: 2915 2552
BlpI GC'TnA_GC
Cuts at: 2042 2884 2042
Size: 842 4625
Bme1580I G_kGCm'C
Cuts at: 1790 2329 3528 4684 1790
Size: 539 1199 1156 2573
Fragments arranged by size:
2573 1199 1156 539
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5467
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2156 3198 4156
Size: 416 53 83 399 24 739 1042 958
Cuts at: 4156 442
Size: 1753
Fragments arranged by size:
1753 1042 958 739 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5467
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2082 2481 4127 1239
Size: 843 399 1646 2579
Fragments arranged by size:
2579 1646 843 399
Bpu10I CC'TnA_GC
Cuts at: 2654 2706 2654
Size: 52 5415
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3592 4457 4755 3592
Size: 865 298 4304
Fragments arranged by size:
4304 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2381 4130 882
Size: 617 882 1749 2219
Fragments arranged by size:
2219 1749 882 617
BsaAI yAC'GTr
Cuts at: 493 1204 2115 3258 493
Size: 711 911 1143 2702
Fragments arranged by size:
2702 1143 911 711
BsaBI GATnn'nnATC
Cuts at: 5461 5461
Size: 5467
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3274 3656 4291 275
Size: 53 83 186 936 1741 382 635 1451
Fragments arranged by size:
1741 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3900 4298 4572 4719 5130 3900
Size: 398 274 147 411 4237
Fragments arranged by size:
4237 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5242
Fragments arranged by size:
5242 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2276 2636 2639 2678 1186
Size: 1090 360 3 39 3975
Fragments arranged by size:
3975 1090 360 39 3
BseYI C'CCAG_C
Cuts at: 2122 2190 2206 4670 5166 2122
Size: 68 16 2464 496 2423
Fragments arranged by size:
2464 2423 496 68 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2089 2771 1163
Size: 573 353 682 3859
Fragments arranged by size:
3859 682 573 353
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2917 3678 4706 1540
Size: 275 186 916 761 1028 2301
Fragments arranged by size:
2301 1028 916 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3528 3613 4684 3528
Size: 85 1071 4311
Fragments arranged by size:
4311 1071 85
BsmI GAATG_Cn'
Cuts at: 2978 3071 2978
Size: 93 5374
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2068 2329 2786 3528 3613 4684 1391
Size: 399 278 261 457 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 457 399 278 261 85
BspEI T'CCGG_A
Cuts at: 5130 5130
Size: 5467
BspHI T'CATG_A
Cuts at: 3356 5086 3356
Size: 1730 3737
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2127 2822 844
Size: 1283 695 3489
Fragments arranged by size:
3489 1283 695
BsrBI CCG'CTC
Cuts at: 1394 1823 3354 1394
Size: 429 1531 3507
Fragments arranged by size:
3507 1531 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3962 4136 65
Size: 1183 2714 174 1396
Fragments arranged by size:
2714 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4111 4298 1700
Size: 110 2301 187 2869
Fragments arranged by size:
2869 2301 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5467
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5467
BssSI C'ACGA_G
Cuts at: 3527 4539 3527
Size: 1012 4455
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5467
BstBI TT'CG_AA
Cuts at: 4271 4271
Size: 5467
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5467
BstYI r'GATC_y
Cuts at: 1972 2547 2797 3164 3549 3566 5018 5104 5116
Size: 575 250 367 385 17 1452 86 12
Cuts at: 5116 5462 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 575 385 367 346 250 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2479 2596 2616 513
Size: 552 939 475 117 20 3364
Fragments arranged by size:
3364 939 552 475 117 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4728
Fragments arranged by size:
4728 683 56
BtsI GCAGTG_nn'
Cuts at: 2239 2760 2983 3777 3797 2239
Size: 521 223 794 20 3909
Fragments arranged by size:
3909 794 521 223 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5467
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5432
DraI TTT'AAA
Cuts at: 2771 2771
Size: 5467
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4474 809
Size: 507 3158 1802
Fragments arranged by size:
3158 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2404 2914 3803
Size: 54 1425 50 275 18 574 510 889
Cuts at: 3803 5432 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 574 510 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2914 1537
Size: 275 1102 4090
Fragments arranged by size:
4090 1102 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3397 1353
Size: 2044 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2506 4054 4428 4574 387
Size: 1064 138 435 482 1548 374 146 1280
Fragments arranged by size:
1548 1280 1064 482 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2082 2481 3530 4127 4914 5182 1239
Size: 393 450 399 1049 597 787 268 1524
Fragments arranged by size:
1524 1049 787 597 450 399 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2325 2325
Size: 5467
EcoRI G'AATT_C
Cuts at: 2776 3154 2776
Size: 378 5089
FspI TGC'GCA
Cuts at: 3973 3973
Size: 5467
HaeII r_GCGC'y
Cuts at: 1678 2180 2377 4614 1678
Size: 502 197 2237 2531
Fragments arranged by size:
2531 2237 502 197
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2055 2087 2120 2152 2342
Size: 32 135 32 483 32 33 32 190
Cuts at: 2342 2346 2374 2378 4108 4140 4182 4214 1373
Size: 4 28 4 1730 32 42 32 2626
Fragments arranged by size:
2626 1730 483 190 135 42 33 32
32 32 32 32 32 28 4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2811 3057 669
Size: 988 281 873 246 3079
Fragments arranged by size:
3079 988 873 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5467
HpaI GTT'AAC
Cuts at: 3057 3057
Size: 5467
KpnI G_GTAC'C
Cuts at: 2792 2792
Size: 5467
MfeI C'AATT_G
Cuts at: 3066 3066
Size: 5467
MluI A'CGCG_T
Cuts at: 5343 5343
Size: 5467
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2364 4581 4765 1305
Size: 1059 2217 184 2007
Fragments arranged by size:
2217 2007 1059 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2406 10
Size: 54 1425 343 574 3071
Fragments arranged by size:
3071 1425 574 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3425 3784 3943 518
Size: 648 2259 359 159 2042
Fragments arranged by size:
2259 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2041 2194 2658 2883 3560 4708 4953 1606
Size: 435 153 464 225 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 464 435 245 225 153
NcoI C'CATG_G
Cuts at: 513 1065 2004 2596 513
Size: 552 939 592 3384
Fragments arranged by size:
3384 939 592 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5467
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5467
NotI GC'GGCC_GC
Cuts at: 2914 2914
Size: 5467
NspI r_CATG'y
Cuts at: 1132 1229 2740 2825 4370 1132
Size: 97 1511 85 1545 2229
Fragments arranged by size:
2229 1545 1511 97 85
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5467
PciI A'CATG_T
Cuts at: 4366 4366
Size: 5467
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2449 1108
Size: 1341 4126
PfoI T'CCnGG_A
Cuts at: 2166 2560 2166
Size: 394 5073
PmeI GTTT'AAAC
Cuts at: 2771 2771
Size: 5467
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3536 3568 5049 5081 3536
Size: 32 1481 32 3922
Fragments arranged by size:
3922 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5467
PsiI TTA'TAA
Cuts at: 3037 3037
Size: 5467
PspOMI G'GGCC_C
Cuts at: 2325 2325
Size: 5467
PstI C_TGCA'G
Cuts at: 830 2819 830
Size: 1989 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5467
PvuII CAG'CTG
Cuts at: 2041 2194 2658 2041
Size: 153 464 4850
Fragments arranged by size:
4850 464 153
SalI G'TCGA_C
Cuts at: 1936 2809 1936
Size: 873 4594
SbfI CC_TGCA'GG
Cuts at: 2819 2819
Size: 5467
ScaI AGT'ACT
Cuts at: 3715 5427 3715
Size: 1712 3755
SfcI C'TryA_G
Cuts at: 826 1045 2815 3950 4631 4822 826
Size: 219 1770 1135 681 191 1471
Fragments arranged by size:
1770 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5467
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5467
SmaI CCC'GGG
Cuts at: 1872 2141 2794 1872
Size: 269 653 4545
Fragments arranged by size:
4545 653 269
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3571 4472 4734 820
Size: 197 926 1628 901 262 1553
Fragments arranged by size:
1628 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3258 493
Size: 2765 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5467
SphI G_CATG'C
Cuts at: 1132 2825 1132
Size: 1693 3774
SspI AAT'ATT
Cuts at: 5 52 3391 5
Size: 47 3339 2081
Fragments arranged by size:
3339 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5467
StyI C'CwwG_G
Cuts at: 513 1065 2004 2596 2906 513
Size: 552 939 592 310 3074
Fragments arranged by size:
3074 939 592 552 310
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2114 2472 2524 2773 3494 3511 3664
Size: 1255 534 358 52 249 721 17 153
Cuts at: 3664 3849 4294 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 721 534 445 358 249 185
153 52 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2017 3713 5425
Size: 274 80 33 51 1443 40 1696 1712
Cuts at: 5425 96
Size: 138
Fragments arranged by size:
1712 1696 1443 274 138 80 51 40
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5427 5459 5427
Size: 32 5435
Tth111I GACn'n_nGTC
Cuts at: 1316 2162 1316
Size: 846 4621
XbaI T'CTAG_A
Cuts at: 2803 2803
Size: 5467
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5467
XmaI C'CCGG_G
Cuts at: 1870 2139 2792 1870
Size: 269 653 4545
Fragments arranged by size:
4545 653 269
XmnI GAAnn'nnTTC
Cuts at: 3596 3596
Size: 5467
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3275 276
Size: 53 83 186 936 1741 2468
Fragments arranged by size:
2468 1741 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI