Restriction map for
pFN21AB8325
(Circular) (Six-base) MAPSORT of: pFN21AB8325.seq Check: 8189 from: 1 to: 5323
pFN21AB8325 5323 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3133 278
Size: 53 83 186 936 1597 2468
Fragments arranged by size:
2468 1597 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2666 1937
Size: 729 4594
Acc65I G'GTAC_C
Cuts at: 2644 2644
Size: 5323
AclI AA'CG_TT
Cuts at: 1563 3450 3823 1563
Size: 1887 373 3063
Fragments arranged by size:
3063 1887 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2051 3386 4770 5038 1632
Size: 419 1335 1384 268 1917
Fragments arranged by size:
1917 1384 1335 419 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5126
AflIII A'CryG_T
Cuts at: 1203 4222 5199 1203
Size: 3019 977 1327
Fragments arranged by size:
3019 1327 977
AgeI A'CCGG_T
Cuts at: 4154 4154
Size: 5323
AhdI GACnn_n'nnGTC
Cuts at: 4052 4052
Size: 5323
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5323
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5323
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2209 2241 2209
Size: 32 5291
AlwNI CAG_nnn'CTG
Cuts at: 3066 4638 3066
Size: 1572 3751
ApaLI G'TGCA_C
Cuts at: 2244 3380 4536 2244
Size: 1136 1156 3031
Fragments arranged by size:
3031 1156 1136
ApoI r'AATT_y
Cuts at: 1493 2417 2632 2858 3010 5119 1493
Size: 924 215 226 152 2109 1697
Fragments arranged by size:
2109 1697 924 226 215 152
AseI AT'TA_AT
Cuts at: 160 1032 3877 160
Size: 872 2845 1606
Fragments arranged by size:
2845 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5323
AvaI C'yCGr_G
Cuts at: 1870 1943 2648 1870
Size: 73 705 4545
Fragments arranged by size:
4545 705 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2277 2310 2277
Size: 33 5290
BamHI G'GATC_C
Cuts at: 2653 3020 2653
Size: 367 4956
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2644 4099 618
Size: 325 217 627 857 1455 1842
Fragments arranged by size:
1842 1455 857 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2642 1391
Size: 1251 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4352
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3514 3548 1903
Size: 34 1577 34 3678
Fragments arranged by size:
3678 1577 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2185 3215 4425 2185
Size: 1030 1210 3083
Fragments arranged by size:
3083 1210 1030
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5323
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3934 136
Size: 107 122 71 631 605 2262 1525
Fragments arranged by size:
2262 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5318 5318
Size: 5323
BlpI GC'TnA_GC
Cuts at: 2740 2740
Size: 5323
Bme1580I G_kGCm'C
Cuts at: 1790 2248 3384 4540 1790
Size: 458 1136 1156 2573
Fragments arranged by size:
2573 1156 1136 458
BmgBI CAC'GTC
Cuts at: 1318 2017 1318
Size: 699 4624
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3054 4012 442
Size: 416 53 83 399 24 1637 958 1753
Fragments arranged by size:
1753 1637 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5323
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3983 1239
Size: 2744 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2101 3448 4313 4611 2101
Size: 1347 865 298 2813
Fragments arranged by size:
2813 1347 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3986 882
Size: 617 2487 2219
Fragments arranged by size:
2487 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3114 493
Size: 711 1910 2702
Fragments arranged by size:
2702 1910 711
BsaBI GATnn'nnATC
Cuts at: 2039 5317 2039
Size: 3278 2045
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3130 3512 4147 275
Size: 53 83 186 936 1597 382 635 1451
Fragments arranged by size:
1597 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3756 4154 4428 4575 4986 3756
Size: 398 274 147 411 4093
Fragments arranged by size:
4093 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2209 2239 1178
Size: 30 165 30 806 30 4262
Fragments arranged by size:
4262 806 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2604 2607 1186
Size: 1418 3 3902
Fragments arranged by size:
3902 1418 3
BseYI C'CCAG_C
Cuts at: 2228 4526 5022 2228
Size: 2298 496 2529
Fragments arranged by size:
2529 2298 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4750
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2773 3534 4562 1540
Size: 275 186 772 761 1028 2301
Fragments arranged by size:
2301 1028 772 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2248 3384 3469 4540 2248
Size: 1136 85 1071 3031
Fragments arranged by size:
3031 1136 1071 85
BsmI GAATG_Cn'
Cuts at: 2431 2834 2927 2431
Size: 403 93 4827
Fragments arranged by size:
4827 403 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2248 2642 3384 3469 4540 1391
Size: 399 458 394 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 458 399 394 85
BspEI T'CCGG_A
Cuts at: 4986 4986
Size: 5323
BspHI T'CATG_A
Cuts at: 3212 4942 3212
Size: 1730 3593
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2678 844
Size: 1834 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3210 1394
Size: 429 1387 3507
Fragments arranged by size:
3507 1387 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3818 3992 65
Size: 1183 2570 174 1396
Fragments arranged by size:
2570 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3967 4154 1700
Size: 110 2157 187 2869
Fragments arranged by size:
2869 2157 187 110
BsrGI T'GTAC_A
Cuts at: 96 2333 2476 96
Size: 2237 143 2943
Fragments arranged by size:
2943 2237 143
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5323
BssSI C'ACGA_G
Cuts at: 3383 4395 3383
Size: 1012 4311
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5323
BstBI TT'CG_AA
Cuts at: 4127 4127
Size: 5323
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5323
BstYI r'GATC_y
Cuts at: 1972 2034 2653 3020 3405 3422 4874 4960 4972
Size: 62 619 367 385 17 1452 86 12
Cuts at: 4972 5318 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 619 385 367 346 86 62
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3832
Fragments arranged by size:
3832 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4584
Fragments arranged by size:
4584 683 56
BtsI GCAGTG_nn'
Cuts at: 2839 3633 3653 2839
Size: 794 20 4509
Fragments arranged by size:
4509 794 20
ClaI AT'CG_AT
Cuts at: 1554 2376 1554
Size: 822 4501
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2139 2174 529
Size: 35 1575 35 3678
Fragments arranged by size:
3678 1575 35 35
DraI TTT'AAA
Cuts at: 2627 2627
Size: 5323
DraIII CAC_nnn'GTG
Cuts at: 2252 2252
Size: 5323
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4330 809
Size: 507 3014 1802
Fragments arranged by size:
3014 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2770 3659 5288
Size: 54 1425 50 275 18 940 889 1629
Cuts at: 5288 8
Size: 43
Fragments arranged by size:
1629 1425 940 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2770 1537
Size: 275 958 4090
Fragments arranged by size:
4090 958 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2275 3253 1353
Size: 922 978 3423
Fragments arranged by size:
3423 978 922
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3910 4284 4430 387
Size: 1064 138 2321 374 146 1280
Fragments arranged by size:
2321 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 3386 3983 4770 5038 1239
Size: 393 419 1335 597 787 268 1524
Fragments arranged by size:
1524 1335 787 597 419 393 268
EcoRI G'AATT_C
Cuts at: 2632 3010 2632
Size: 378 4945
FspI TGC'GCA
Cuts at: 3829 3829
Size: 5323
HaeII r_GCGC'y
Cuts at: 1678 4470 1678
Size: 2792 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3964 3996 4038 4070 1373
Size: 32 135 32 2392 32 42 32 2626
Fragments arranged by size:
2626 2392 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2134 2667 2913 669
Size: 988 281 196 533 246 3079
Fragments arranged by size:
3079 988 533 281 246 196
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5323
HpaI GTT'AAC
Cuts at: 2913 2913
Size: 5323
KpnI G_GTAC'C
Cuts at: 2648 2648
Size: 5323
MfeI C'AATT_G
Cuts at: 2922 2922
Size: 5323
MluI A'CGCG_T
Cuts at: 5199 5199
Size: 5323
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2344 4437 4621 1305
Size: 1039 2093 184 2007
Fragments arranged by size:
2093 2007 1039 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3501
Fragments arranged by size:
3501 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3281 3640 3799 518
Size: 648 2115 359 159 2042
Fragments arranged by size:
2115 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2739 3416 4564 4809 1606
Size: 1133 677 1148 245 2120
Fragments arranged by size:
2120 1148 1133 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3832
Fragments arranged by size:
3832 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5323
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5323
NotI GC'GGCC_GC
Cuts at: 2770 2770
Size: 5323
NspI r_CATG'y
Cuts at: 1132 1229 2359 2681 4226 1132
Size: 97 1130 322 1545 2229
Fragments arranged by size:
2229 1545 1130 322 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5323
PciI A'CATG_T
Cuts at: 4222 4222
Size: 5323
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5323
PmeI GTTT'AAAC
Cuts at: 2627 2627
Size: 5323
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2209 2241 3392 3424 4905 4937 2209
Size: 32 1151 32 1481 32 2595
Fragments arranged by size:
2595 1481 1151 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5323
PsiI TTA'TAA
Cuts at: 2893 2893
Size: 5323
PstI C_TGCA'G
Cuts at: 830 2675 830
Size: 1845 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5323
SalI G'TCGA_C
Cuts at: 1936 2665 1936
Size: 729 4594
SbfI CC_TGCA'GG
Cuts at: 2675 2675
Size: 5323
ScaI AGT'ACT
Cuts at: 3571 5283 3571
Size: 1712 3611
SfcI C'TryA_G
Cuts at: 826 1045 2671 3806 4487 4678 826
Size: 219 1626 1135 681 191 1471
Fragments arranged by size:
1626 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5323
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5323
SmaI CCC'GGG
Cuts at: 1872 2650 1872
Size: 778 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2080 3427 4328 4590 820
Size: 197 926 137 1347 901 262 1553
Fragments arranged by size:
1553 1347 926 901 262 197 137
SnaBI TAC'GTA
Cuts at: 493 3114 493
Size: 2621 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5323
SphI G_CATG'C
Cuts at: 1132 2681 1132
Size: 1549 3774
SspI AAT'ATT
Cuts at: 5 52 3247 5
Size: 47 3195 2081
Fragments arranged by size:
3195 2081 47
StuI AGG'CCT
Cuts at: 1517 2412 1517
Size: 895 4428
StyI C'CwwG_G
Cuts at: 513 1065 2004 2584 2762 513
Size: 552 939 580 178 3074
Fragments arranged by size:
3074 939 580 552 178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3350 3367 3520 3705 4150 325
Size: 1255 1770 17 153 185 445 1498
Fragments arranged by size:
1770 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2333 2476 3569
Size: 274 80 33 51 1443 356 143 1093
Cuts at: 3569 5281 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1093 356 274 143 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5283 5315 5283
Size: 32 5291
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5323
XbaI T'CTAG_A
Cuts at: 2659 2659
Size: 5323
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5323
XmaI C'CCGG_G
Cuts at: 1870 2648 1870
Size: 778 4545
XmnI GAAnn'nnTTC
Cuts at: 2431 3452 2431
Size: 1021 4302
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3131 276
Size: 53 83 186 936 1597 2468
Fragments arranged by size:
2468 1597 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI