Restriction map for
pFN21AB8319
(Circular) (Six-base) MAPSORT of: pFN21AB8319.seq Check: 867 from: 1 to: 5602
pFN21AB8319 5602 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3412 278
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2945 1937
Size: 1008 4594
Acc65I G'GTAC_C
Cuts at: 2923 2923
Size: 5602
AclI AA'CG_TT
Cuts at: 1563 3729 4102 1563
Size: 2166 373 3063
Fragments arranged by size:
3063 2166 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2270 2502 3665 5049 5317 1632
Size: 638 232 1163 1384 268 1917
Fragments arranged by size:
1917 1384 1163 638 268 232
AflII C'TTAA_G
Cuts at: 820 1017 2486 820
Size: 197 1469 3936
Fragments arranged by size:
3936 1469 197
AflIII A'CryG_T
Cuts at: 1203 4501 5478 1203
Size: 3298 977 1327
Fragments arranged by size:
3298 1327 977
AgeI A'CCGG_T
Cuts at: 4433 4433
Size: 5602
AhdI GACnn_n'nnGTC
Cuts at: 4331 4331
Size: 5602
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5602
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2201 1184
Size: 1017 4585
AlwNI CAG_nnn'CTG
Cuts at: 3345 4917 3345
Size: 1572 4030
ApaLI G'TGCA_C
Cuts at: 3659 4815 3659
Size: 1156 4446
ApoI r'AATT_y
Cuts at: 1493 2911 3137 3289 5398 1493
Size: 1418 226 152 2109 1697
Fragments arranged by size:
2109 1697 1418 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4156 160
Size: 872 3124 1606
Fragments arranged by size:
3124 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
AvaI C'yCGr_G
Cuts at: 1870 1943 2927 1870
Size: 73 984 4545
Fragments arranged by size:
4545 984 73
BamHI G'GATC_C
Cuts at: 2220 2623 2932 3299 2220
Size: 403 309 367 4523
Fragments arranged by size:
4523 403 367 309
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2923 4378 618
Size: 325 217 627 1136 1455 1842
Fragments arranged by size:
1842 1455 1136 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2367 2768 2921 1391
Size: 976 401 153 4072
Fragments arranged by size:
4072 976 401 153
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2300 2502 928
Size: 971 401 202 4028
Fragments arranged by size:
4028 971 401 202
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3793 3827 1903
Size: 34 1856 34 3678
Fragments arranged by size:
3678 1856 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3494 4704 3494
Size: 1210 4392
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5602
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4213 136
Size: 107 122 71 631 605 2541 1525
Fragments arranged by size:
2541 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5597 5597
Size: 5602
BlpI GC'TnA_GC
Cuts at: 3019 3019
Size: 5602
Bme1580I G_kGCm'C
Cuts at: 1790 3663 4819 1790
Size: 1873 1156 2573
Fragments arranged by size:
2573 1873 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5602
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2060 3333 4291
Size: 416 53 83 399 24 643 1273 958
Cuts at: 4291 442
Size: 1753
Fragments arranged by size:
1753 1273 958 643 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5602
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4262 1239
Size: 3023 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3727 4592 4890 3727
Size: 865 298 4439
Fragments arranged by size:
4439 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4265 882
Size: 617 2766 2219
Fragments arranged by size:
2766 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3393 493
Size: 711 2189 2702
Fragments arranged by size:
2702 2189 711
BsaBI GATnn'nnATC
Cuts at: 2886 5596 2886
Size: 2710 2892
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3409 3791 4426 275
Size: 53 83 186 936 1876 382 635 1451
Fragments arranged by size:
1876 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4035 4433 4707 4854 5265 4035
Size: 398 274 147 411 4372
Fragments arranged by size:
4372 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5377
Fragments arranged by size:
5377 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5602
BseYI C'CCAG_C
Cuts at: 4805 5301 4805
Size: 496 5106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2171 2695 2812 1163
Size: 573 435 524 117 3953
Fragments arranged by size:
3953 573 524 435 117
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3052 3813 4841 1540
Size: 275 186 1051 761 1028 2301
Fragments arranged by size:
2301 1051 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2768 3663 3748 4819 2768
Size: 895 85 1071 3551
Fragments arranged by size:
3551 1071 895 85
BsmI GAATG_Cn'
Cuts at: 3113 3206 3113
Size: 93 5509
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2367 2768 2921 3663 3748 4819 1391
Size: 399 577 401 153 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 577 401 399 153 85
BspEI T'CCGG_A
Cuts at: 5265 5265
Size: 5602
BspHI T'CATG_A
Cuts at: 2410 3491 5221 2410
Size: 1081 1730 2791
Fragments arranged by size:
2791 1730 1081
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2433 2536 2957 844
Size: 1589 103 421 3489
Fragments arranged by size:
3489 1589 421 103
BsrBI CCG'CTC
Cuts at: 1394 1823 3489 1394
Size: 429 1666 3507
Fragments arranged by size:
3507 1666 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2122 2165 4097 4271 65
Size: 1183 874 43 1932 174 1396
Fragments arranged by size:
1932 1396 1183 874 174 43
BsrFI r'CCGG_y
Cuts at: 1700 1810 2111 4246 4433 1700
Size: 110 301 2135 187 2869
Fragments arranged by size:
2869 2135 301 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5602
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5602
BssSI C'ACGA_G
Cuts at: 3662 4674 3662
Size: 1012 4590
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2155 794
Size: 1361 4241
BstBI TT'CG_AA
Cuts at: 4406 4406
Size: 5602
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5602
BstXI CCAn_nnnn'nTGG
Cuts at: 2578 2578
Size: 5602
BstYI r'GATC_y
Cuts at: 1972 2019 2220 2596 2623 2647 2932 3299 3684
Size: 47 201 376 27 24 285 367 385
Cuts at: 3684 3701 5153 5239 5251 5597 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 376 367 346 285 201
86 47 27 24 17 12
Bsu36I CC'TnA_GG
Cuts at: 2376 2376
Size: 5602
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4111
Fragments arranged by size:
4111 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2820 487
Size: 683 56 1594 3269
Fragments arranged by size:
3269 1594 683 56
BtsI GCAGTG_nn'
Cuts at: 2049 3118 3912 3932 2049
Size: 1069 794 20 3719
Fragments arranged by size:
3719 1069 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5602
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5567
DraI TTT'AAA
Cuts at: 2906 2906
Size: 5602
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4609 809
Size: 507 3293 1802
Fragments arranged by size:
3293 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2029 3049 3938
Size: 54 1425 50 275 18 199 1020 889
Cuts at: 3938 5567 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1020 889 275 199 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3049 1537
Size: 275 1237 4090
Fragments arranged by size:
4090 1237 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3532 1353
Size: 2179 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4189 4563 4709 387
Size: 1064 138 2600 374 146 1280
Fragments arranged by size:
2600 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2766 2766
Size: 5602
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2270 2502 3665 4262 5049 5317 1239
Size: 393 638 232 1163 597 787 268 1524
Fragments arranged by size:
1524 1163 787 638 597 393 268 232
EcoRI G'AATT_C
Cuts at: 2911 3289 2911
Size: 378 5224
FspI TGC'GCA
Cuts at: 4108 4108
Size: 5602
HaeII r_GCGC'y
Cuts at: 1678 4749 1678
Size: 3071 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2045 2077 4243 4275 4317
Size: 32 135 32 473 32 2166 32 42
Cuts at: 4317 4349 1373
Size: 32 2626
Fragments arranged by size:
2626 2166 473 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2109 2946 3192 669
Size: 988 281 171 837 246 3079
Fragments arranged by size:
3079 988 837 281 246 171
HindIII A'AGCT_T
Cuts at: 748 2096 2489 748
Size: 1348 393 3861
Fragments arranged by size:
3861 1348 393
HpaI GTT'AAC
Cuts at: 2109 3192 2109
Size: 1083 4519
KpnI G_GTAC'C
Cuts at: 2927 2927
Size: 5602
MfeI C'AATT_G
Cuts at: 3201 3201
Size: 5602
MluI A'CGCG_T
Cuts at: 5478 5478
Size: 5602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4716 4900 1305
Size: 3411 184 2007
Fragments arranged by size:
3411 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2031 10
Size: 54 1425 343 199 3581
Fragments arranged by size:
3581 1425 343 199 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2415 3560 3919 4078 518
Size: 648 1249 1145 359 159 2042
Fragments arranged by size:
2042 1249 1145 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2152 2270 2359 2817 2826 3018 3695 4843
Size: 546 118 89 458 9 192 677 1148
Cuts at: 4843 5088 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 546 458 245 192 118
89 9
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4111
Fragments arranged by size:
4111 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5602
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5602
NotI GC'GGCC_GC
Cuts at: 3049 3049
Size: 5602
NspI r_CATG'y
Cuts at: 1132 1229 2960 4505 1132
Size: 97 1731 1545 2229
Fragments arranged by size:
2229 1731 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5602
PciI A'CATG_T
Cuts at: 4501 4501
Size: 5602
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2038 2556 1108
Size: 930 518 4154
Fragments arranged by size:
4154 930 518
PmeI GTTT'AAAC
Cuts at: 2906 2906
Size: 5602
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3671 3703 5184 5216 3671
Size: 32 1481 32 4057
Fragments arranged by size:
4057 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5602
PsiI TTA'TAA
Cuts at: 3172 3172
Size: 5602
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2376 2408 2376
Size: 32 5570
PstI C_TGCA'G
Cuts at: 830 2358 2954 830
Size: 1528 596 3478
Fragments arranged by size:
3478 1528 596
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5602
PvuII CAG'CTG
Cuts at: 2152 2270 2826 2152
Size: 118 556 4928
Fragments arranged by size:
4928 556 118
SacI G_AGCT'C
Cuts at: 2768 2768
Size: 5602
SalI G'TCGA_C
Cuts at: 1936 2944 1936
Size: 1008 4594
SbfI CC_TGCA'GG
Cuts at: 2954 2954
Size: 5602
ScaI AGT'ACT
Cuts at: 3850 5562 3850
Size: 1712 3890
SfcI C'TryA_G
Cuts at: 826 1045 2271 2354 2950 4085 4766 4957 826
Size: 219 1226 83 596 1135 681 191 1471
Fragments arranged by size:
1471 1226 1135 681 596 219 191 83
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5602
SmaI CCC'GGG
Cuts at: 1872 2929 1872
Size: 1057 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2486 3706 4607 4869 820
Size: 197 926 543 1220 901 262 1553
Fragments arranged by size:
1553 1220 926 901 543 262 197
SnaBI TAC'GTA
Cuts at: 493 3393 493
Size: 2900 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5602
SphI G_CATG'C
Cuts at: 1132 2960 1132
Size: 1828 3774
SspI AAT'ATT
Cuts at: 5 52 3526 5
Size: 47 3474 2081
Fragments arranged by size:
3474 2081 47
StuI AGG'CCT
Cuts at: 1517 2699 1517
Size: 1182 4420
StyI C'CwwG_G
Cuts at: 513 1065 2004 2404 2836 3041 513
Size: 552 939 400 432 205 3074
Fragments arranged by size:
3074 939 552 432 400 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3629 3646 3799 3984 4429 325
Size: 1255 2049 17 153 185 445 1498
Fragments arranged by size:
2049 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2383 3848 5560
Size: 274 80 33 51 1443 406 1465 1712
Cuts at: 5560 96
Size: 138
Fragments arranged by size:
1712 1465 1443 406 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5562 5594 5562
Size: 32 5570
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5602
XbaI T'CTAG_A
Cuts at: 2938 2938
Size: 5602
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5602
XmaI C'CCGG_G
Cuts at: 1870 2927 1870
Size: 1057 4545
XmnI GAAnn'nnTTC
Cuts at: 3731 3731
Size: 5602
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3410 276
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI