Restriction map for
pFN21AB8314
(Circular) (Six-base) MAPSORT of: pFN21AB8314.seq Check: 7021 from: 1 to: 5809
pFN21AB8314 5809 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2223 2223
Size: 5809
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3619 278
Size: 53 83 186 936 2083 2468
Fragments arranged by size:
2468 2083 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3152 1937
Size: 1215 4594
Acc65I G'GTAC_C
Cuts at: 3130 3130
Size: 5809
AclI AA'CG_TT
Cuts at: 1563 3936 4309 1563
Size: 2373 373 3063
Fragments arranged by size:
3063 2373 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2267 2389 2648 3872 5256 5524 1632
Size: 635 122 259 1224 1384 268 1917
Fragments arranged by size:
1917 1384 1224 635 268 259 122
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5612
AflIII A'CryG_T
Cuts at: 1203 4708 5685 1203
Size: 3505 977 1327
Fragments arranged by size:
3505 1327 977
AgeI A'CCGG_T
Cuts at: 4640 4640
Size: 5809
AhdI GACnn_n'nnGTC
Cuts at: 4538 4538
Size: 5809
AleI CACnn'nnGTG
Cuts at: 1166 2945 1166
Size: 1779 4030
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5809
AlwNI CAG_nnn'CTG
Cuts at: 2053 3552 5124 2053
Size: 1499 1572 2738
Fragments arranged by size:
2738 1572 1499
ApaLI G'TGCA_C
Cuts at: 3866 5022 3866
Size: 1156 4653
ApoI r'AATT_y
Cuts at: 1493 3118 3344 3496 5605 1493
Size: 1625 226 152 2109 1697
Fragments arranged by size:
2109 1697 1625 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4363 160
Size: 872 3331 1606
Fragments arranged by size:
3331 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5809
AvaI C'yCGr_G
Cuts at: 1870 1943 3134 1870
Size: 73 1191 4545
Fragments arranged by size:
4545 1191 73
AvrII C'CTAG_G
Cuts at: 2782 2782
Size: 5809
BamHI G'GATC_C
Cuts at: 3139 3506 3139
Size: 367 5442
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2019 2524 2878 3130 4585
Size: 325 217 627 232 505 354 252 1455
Cuts at: 4585 618
Size: 1842
Fragments arranged by size:
1842 1455 627 505 354 325 252 232
217
BanII G_rGCy'C
Cuts at: 1391 2319 2496 2550 2850 2892 3128 1391
Size: 928 177 54 300 42 236 4072
Fragments arranged by size:
4072 928 300 236 177 54 42
BbeI G_GCGC'C
Cuts at: 2023 2023
Size: 5809
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4838
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4000 4034 1903
Size: 34 2063 34 3678
Fragments arranged by size:
3678 2063 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3701 4911 3701
Size: 1210 4599
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5809
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2017 2887 4420
Size: 107 122 71 631 605 345 870 1533
Cuts at: 4420 136
Size: 1525
Fragments arranged by size:
1533 1525 870 631 605 345 122 107
71
BglII A'GATC_T
Cuts at: 2265 2379 5804 2265
Size: 114 3425 2270
Fragments arranged by size:
3425 2270 114
BlpI GC'TnA_GC
Cuts at: 3226 3226
Size: 5809
Bme1580I G_kGCm'C
Cuts at: 1790 2259 2529 2883 3033 3870 5026 1790
Size: 469 270 354 150 837 1156 2573
Fragments arranged by size:
2573 1156 837 469 354 270 150
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5809
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3046 3540 4498
Size: 416 53 83 399 24 1629 494 958
Cuts at: 4498 442
Size: 1753
Fragments arranged by size:
1753 1629 958 494 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5809
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2327 2609 2631 2762 2894 4469 1239
Size: 1088 282 22 131 132 1575 2579
Fragments arranged by size:
2579 1575 1088 282 132 131 22
Bpu10I CC'TnA_GC
Cuts at: 2042 2822 2042
Size: 780 5029
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2885 3934 4799 5097 2885
Size: 1049 865 298 3597
Fragments arranged by size:
3597 1049 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2345 2684 4472 882
Size: 617 846 339 1788 2219
Fragments arranged by size:
2219 1788 846 617 339
BsaAI yAC'GTr
Cuts at: 493 1204 3600 493
Size: 711 2396 2702
Fragments arranged by size:
2702 2396 711
BsaBI GATnn'nnATC
Cuts at: 5803 5803
Size: 5809
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2020 2148 3616 3998
Size: 53 83 186 936 487 128 1468 382
Cuts at: 3998 4633 275
Size: 635 1451
Fragments arranged by size:
1468 1451 936 635 487 382 186 128
83 53
BsaWI w'CCGG_w
Cuts at: 2550 4242 4640 4914 5061 5472 2550
Size: 1692 398 274 147 411 2887
Fragments arranged by size:
2887 1692 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2294 2324 2366 2396 1178
Size: 30 165 30 891 30 42 30 4591
Fragments arranged by size:
4591 891 165 42 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2897 1186
Size: 1711 4098
BseYI C'CCAG_C
Cuts at: 2477 2776 5012 5508 2477
Size: 299 2236 496 2778
Fragments arranged by size:
2778 2236 496 299
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2909 3065 1163
Size: 573 1173 156 3907
Fragments arranged by size:
3907 1173 573 156
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3259 4020 5048 1540
Size: 275 186 1258 761 1028 2301
Fragments arranged by size:
2301 1258 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2234 2319 2496 2850 3870 3955 5026 2234
Size: 85 177 354 1020 85 1071 3017
Fragments arranged by size:
3017 1071 1020 354 177 85 85
BsmI GAATG_Cn'
Cuts at: 3320 3413 3320
Size: 93 5716
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2234 2259 2319 2496 2529 2550 2850
Size: 399 444 25 60 177 33 21 300
Cuts at: 2850 2883 2892 3033 3128 3870 3955 5026 1391
Size: 33 9 141 95 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 444 399 300 177 141
95 85 60 33 33 25 21 9
BspEI T'CCGG_A
Cuts at: 2550 5472 2550
Size: 2922 2887
BspHI T'CATG_A
Cuts at: 2106 3698 5428 2106
Size: 1592 1730 2487
Fragments arranged by size:
2487 1730 1592
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2223 2301 2649 2757 3164 844
Size: 1379 78 348 108 407 3489
Fragments arranged by size:
3489 1379 407 348 108 78
BsrBI CCG'CTC
Cuts at: 1394 1823 3696 1394
Size: 429 1873 3507
Fragments arranged by size:
3507 1873 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4304 4478 65
Size: 1183 3056 174 1396
Fragments arranged by size:
3056 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3049 4453 4640 1700
Size: 110 1239 1404 187 2869
Fragments arranged by size:
2869 1404 1239 187 110
BsrGI T'GTAC_A
Cuts at: 96 2769 96
Size: 2673 3136
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5809
BssSI C'ACGA_G
Cuts at: 2024 3869 4881 2024
Size: 1845 1012 2952
Fragments arranged by size:
2952 1845 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5809
BstBI TT'CG_AA
Cuts at: 4613 4613
Size: 5809
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5809
BstXI CCAn_nnnn'nTGG
Cuts at: 2287 2485 2740 2287
Size: 198 255 5356
Fragments arranged by size:
5356 255 198
BstYI r'GATC_y
Cuts at: 1972 2265 2379 2388 2460 3139 3506 3891 3908
Size: 293 114 9 72 679 367 385 17
Cuts at: 3908 5360 5446 5458 5804 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 679 385 367 346 293 114
86 72 17 12 9
Bsu36I CC'TnA_GG
Cuts at: 2143 2884 2143
Size: 741 5068
BtgI C'CryG_G
Cuts at: 513 1065 2970 513
Size: 552 1905 3352
Fragments arranged by size:
3352 1905 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5070
Fragments arranged by size:
5070 683 56
BtsI GCAGTG_nn'
Cuts at: 2206 2411 3325 4119 4139 2206
Size: 205 914 794 20 3876
Fragments arranged by size:
3876 914 794 205 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5809
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5774
DraI TTT'AAA
Cuts at: 3113 3113
Size: 5809
DraIII CAC_nnn'GTG
Cuts at: 2455 2455
Size: 5809
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4816 809
Size: 507 3500 1802
Fragments arranged by size:
3500 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2871 3051 3081
Size: 54 1425 50 275 18 1041 180 30
Cuts at: 3081 3256 4145 5774 8
Size: 175 889 1629 43
Fragments arranged by size:
1629 1425 1041 889 275 180 175 54
50 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 3256 1537
Size: 275 1444 4090
Fragments arranged by size:
4090 1444 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3020 3739 1353
Size: 1667 719 3423
Fragments arranged by size:
3423 1667 719
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4396 4770 4916 387
Size: 1064 138 2807 374 146 1280
Fragments arranged by size:
2807 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2317 2494 2848 2317
Size: 177 354 5278
Fragments arranged by size:
5278 354 177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2267 2327 2389 2609 2631 2648 2762
Size: 393 635 60 62 220 22 17 114
Cuts at: 2762 2894 3872 4469 5256 5524 1239
Size: 132 978 597 787 268 1524
Fragments arranged by size:
1524 978 787 635 597 393 268 220
132 114 62 60 22 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2045 2045
Size: 5809
EcoO109I rG'GnC_Cy
Cuts at: 2050 2746 2050
Size: 696 5113
EcoRI G'AATT_C
Cuts at: 3118 3496 3118
Size: 378 5431
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2347 2347
Size: 5809
FspI TGC'GCA
Cuts at: 2645 4315 2645
Size: 1670 4139
HaeII r_GCGC'y
Cuts at: 1678 2023 2136 2187 4956 1678
Size: 345 113 51 2769 2531
Fragments arranged by size:
2769 2531 345 113 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2147 2179 4450 4482 4524
Size: 32 135 32 575 32 2271 32 42
Cuts at: 4524 4556 1373
Size: 32 2626
Fragments arranged by size:
2626 2271 575 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2956 3153 3399 669
Size: 988 281 1018 197 246 3079
Fragments arranged by size:
3079 1018 988 281 246 197
HindIII A'AGCT_T
Cuts at: 748 2124 748
Size: 1376 4433
HpaI GTT'AAC
Cuts at: 3399 3399
Size: 5809
KasI G'GCGC_C
Cuts at: 2019 2019
Size: 5809
KpnI G_GTAC'C
Cuts at: 3134 3134
Size: 5809
MfeI C'AATT_G
Cuts at: 3408 3408
Size: 5809
MluI A'CGCG_T
Cuts at: 5685 5685
Size: 5809
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2259 2955 4923 5107 1305
Size: 954 696 1968 184 2007
Fragments arranged by size:
2007 1968 954 696 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2873 3083 10
Size: 54 1425 343 1041 210 2736
Fragments arranged by size:
2736 1425 1041 343 210 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2570 2945 3767 4126 4285 518
Size: 648 1404 375 822 359 159 2042
Fragments arranged by size:
2042 1404 822 648 375 359 159
MspA1I CmG'CkG
Cuts at: 1606 2544 3063 3225 3902 5050 5295 1606
Size: 938 519 162 677 1148 245 2120
Fragments arranged by size:
2120 1148 938 677 519 245 162
NaeI GCC'GGC
Cuts at: 3051 3051
Size: 5809
NarI GG'CG_CC
Cuts at: 2020 2020
Size: 5809
NcoI C'CATG_G
Cuts at: 513 1065 2970 513
Size: 552 1905 3352
Fragments arranged by size:
3352 1905 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5809
NgoMIV G'CCGG_C
Cuts at: 3049 3049
Size: 5809
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5809
NotI GC'GGCC_GC
Cuts at: 3256 3256
Size: 5809
NspI r_CATG'y
Cuts at: 1132 1229 3167 4712 1132
Size: 97 1938 1545 2229
Fragments arranged by size:
2229 1938 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5809
PciI A'CATG_T
Cuts at: 4708 4708
Size: 5809
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2455 1108
Size: 1347 4462
PfoI T'CCnGG_A
Cuts at: 2151 2841 2151
Size: 690 5119
PmeI GTTT'AAAC
Cuts at: 3113 3113
Size: 5809
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3878 3910 5391 5423 3878
Size: 32 1481 32 4264
Fragments arranged by size:
4264 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2746 2746
Size: 5809
PshAI GACnn'nnGTC
Cuts at: 1613 2241 2964 1613
Size: 628 723 4458
Fragments arranged by size:
4458 723 628
PsiI TTA'TAA
Cuts at: 3379 3379
Size: 5809
PstI C_TGCA'G
Cuts at: 830 2298 2427 3161 830
Size: 1468 129 734 3478
Fragments arranged by size:
3478 1468 734 129
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5809
SacI G_AGCT'C
Cuts at: 2319 2496 2850 2319
Size: 177 354 5278
Fragments arranged by size:
5278 354 177
SalI G'TCGA_C
Cuts at: 1936 3151 1936
Size: 1215 4594
SbfI CC_TGCA'GG
Cuts at: 3161 3161
Size: 5809
ScaI AGT'ACT
Cuts at: 4057 5769 4057
Size: 1712 4097
SexAI A'CCwGG_T
Cuts at: 2451 2451
Size: 5809
SfcI C'TryA_G
Cuts at: 826 1045 2179 2294 2423 3157 4292 4973 5164
Size: 219 1134 115 129 734 1135 681 191
Cuts at: 5164 826
Size: 1471
Fragments arranged by size:
1471 1135 1134 734 681 219 191 129
115
SfoI GGC'GCC
Cuts at: 2021 2021
Size: 5809
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5809
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5809
SmaI CCC'GGG
Cuts at: 1872 3136 1872
Size: 1264 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2900 3913 4814 5076 820
Size: 197 926 957 1013 901 262 1553
Fragments arranged by size:
1553 1013 957 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3600 493
Size: 3107 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5809
SphI G_CATG'C
Cuts at: 1132 3167 1132
Size: 2035 3774
SspI AAT'ATT
Cuts at: 5 52 3733 5
Size: 47 3681 2081
Fragments arranged by size:
3681 2081 47
StuI AGG'CCT
Cuts at: 1517 2402 1517
Size: 885 4924
StyI C'CwwG_G
Cuts at: 513 1065 2529 2782 2970 3248 513
Size: 552 1464 253 188 278 3074
Fragments arranged by size:
3074 1464 552 278 253 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2188 2939 3836 3853 4006 4191 4636
Size: 1255 608 751 897 17 153 185 445
Cuts at: 4636 325
Size: 1498
Fragments arranged by size:
1498 1255 897 751 608 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2769 4055 5767
Size: 274 80 33 51 1443 792 1286 1712
Cuts at: 5767 96
Size: 138
Fragments arranged by size:
1712 1443 1286 792 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5769 5801 5769
Size: 32 5777
Tth111I GACn'n_nGTC
Cuts at: 1316 2165 1316
Size: 849 4960
XbaI T'CTAG_A
Cuts at: 3145 3145
Size: 5809
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2449 2485 2449
Size: 36 5773
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5809
XmaI C'CCGG_G
Cuts at: 1870 3134 1870
Size: 1264 4545
XmnI GAAnn'nnTTC
Cuts at: 2402 3938 2402
Size: 1536 4273
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3617 276
Size: 53 83 186 936 2083 2468
Fragments arranged by size:
2468 2083 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I EcoRV FseI FspAI NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SfiI SrfI SwaI