Restriction map for
pFN21AB8303
(Circular) (Six-base) MAPSORT of: pFN21AB8303.seq Check: 5122 from: 1 to: 6505
pFN21AB8303 6505 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3121 3142 3548 3561 3121
Size: 21 406 13 6065
Fragments arranged by size:
6065 406 21 13
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4315 278
Size: 53 83 186 936 2779 2468
Fragments arranged by size:
2779 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2340 2526 3594 3848 1937
Size: 403 186 1068 254 4594
Fragments arranged by size:
4594 1068 403 254 186
Acc65I G'GTAC_C
Cuts at: 3826 3826
Size: 6505
AclI AA'CG_TT
Cuts at: 1563 4632 5005 1563
Size: 3069 373 3063
Fragments arranged by size:
3069 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2227 2690 2807 3293 3407 3638 3797 4568
Size: 595 463 117 486 114 231 159 771
Cuts at: 4568 5952 6220 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 771 595 486 463 268 231
159 117 114
AfeI AGC'GCT
Cuts at: 2648 2648
Size: 6505
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6308
AflIII A'CryG_T
Cuts at: 1203 2898 3162 5404 6381 1203
Size: 1695 264 2242 977 1327
Fragments arranged by size:
2242 1695 1327 977 264
AgeI A'CCGG_T
Cuts at: 5336 5336
Size: 6505
AhdI GACnn_n'nnGTC
Cuts at: 5234 5234
Size: 6505
AleI CACnn'nnGTG
Cuts at: 1166 3713 1166
Size: 2547 3958
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3731 1184
Size: 2547 3958
AlwNI CAG_nnn'CTG
Cuts at: 2519 3004 3136 3409 4248 5820 2519
Size: 485 132 273 839 1572 3204
Fragments arranged by size:
3204 1572 839 485 273 132
ApaLI G'TGCA_C
Cuts at: 3548 4562 5718 3548
Size: 1014 1156 4335
Fragments arranged by size:
4335 1156 1014
ApoI r'AATT_y
Cuts at: 1493 3814 4040 4192 6301 1493
Size: 2321 226 152 2109 1697
Fragments arranged by size:
2321 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5059 160
Size: 872 4027 1606
Fragments arranged by size:
4027 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6505
AvaI C'yCGr_G
Cuts at: 1870 1943 3113 3830 1870
Size: 73 1170 717 4545
Fragments arranged by size:
4545 1170 717 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2705 2738 2705
Size: 33 6472
BamHI G'GATC_C
Cuts at: 3835 4202 3835
Size: 367 6138
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2791 3074 3446 3707 3826
Size: 325 217 627 1004 283 372 261 119
Cuts at: 3826 5281 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1004 627 372 325 283 261
217 119
BanII G_rGCy'C
Cuts at: 1391 2513 2748 3297 3824 1391
Size: 1122 235 549 527 4072
Fragments arranged by size:
4072 1122 549 527 235
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5534
BbvCI CC'TCA_GC
Cuts at: 3095 3095
Size: 6505
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4696 4730 1903
Size: 34 50 34 2675 34 3678
Fragments arranged by size:
3678 2675 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4397 5607 4397
Size: 1210 5295
BclI T'GATC_A
Cuts at: 1548 2196 2379 3453 1548
Size: 648 183 1074 4600
Fragments arranged by size:
4600 1074 648 183
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5116 136
Size: 107 122 71 631 605 3444 1525
Fragments arranged by size:
3444 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3009 6500 3009
Size: 3491 3014
BlpI GC'TnA_GC
Cuts at: 3519 3922 3519
Size: 403 6102
Bme1580I G_kGCm'C
Cuts at: 1790 2233 2796 3077 3552 4566 5722 1790
Size: 443 563 281 475 1014 1156 2573
Fragments arranged by size:
2573 1156 1014 563 475 443 281
BmgBI CAC'GTC
Cuts at: 1318 2047 1318
Size: 729 5776
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4236 5194 442
Size: 416 53 83 399 24 2819 958 1753
Fragments arranged by size:
2819 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6505
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2336 2468 2780 3158 3662 5165 1239
Size: 1097 132 312 378 504 1503 2579
Fragments arranged by size:
2579 1503 1097 504 378 312 132
Bpu10I CC'TnA_GC
Cuts at: 3095 3095
Size: 6505
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3590 4630 5495 5793 3590
Size: 1040 865 298 4302
Fragments arranged by size:
4302 1040 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2074 2678 2756 3730 5168 882
Size: 617 575 604 78 974 1438 2219
Fragments arranged by size:
2219 1438 974 617 604 575 78
BsaAI yAC'GTr
Cuts at: 493 1204 2476 2619 4296 493
Size: 711 1272 143 1677 2702
Fragments arranged by size:
2702 1677 1272 711 143
BsaBI GATnn'nnATC
Cuts at: 6499 6499
Size: 6505
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3186 4312 4694 5329
Size: 53 83 186 936 1653 1126 382 635
Cuts at: 5329 275
Size: 1451
Fragments arranged by size:
1653 1451 1126 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3015 4938 5336 5610 5757 6168 3015
Size: 1923 398 274 147 411 3352
Fragments arranged by size:
3352 1923 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3149 3179 1178
Size: 30 165 30 1746 30 4504
Fragments arranged by size:
4504 1746 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3665 3761 1186
Size: 2479 96 3930
Fragments arranged by size:
3930 2479 96
BseYI C'CCAG_C
Cuts at: 2479 2728 2993 3353 3376 3508 3751 3800 5708
Size: 249 265 360 23 132 243 49 1908
Cuts at: 5708 6204 2479
Size: 496 2780
Fragments arranged by size:
2780 1908 496 360 265 249 243 132
49 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2909 2972 3089 3425 3653 1163
Size: 573 1173 63 117 336 228 4015
Fragments arranged by size:
4015 1173 573 336 228 117 63
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2131 2170 2932 3955 4716 5744
Size: 275 186 130 39 762 1023 761 1028
Cuts at: 5744 1540
Size: 2301
Fragments arranged by size:
2301 1028 1023 762 761 275 186 130
39
BsiHKAI G_wGCw'C
Cuts at: 2748 3297 3525 3552 4566 4651 5722 2748
Size: 549 228 27 1014 85 1071 3531
Fragments arranged by size:
3531 1071 1014 549 228 85 27
BsiWI C'GTAC_G
Cuts at: 2619 2619
Size: 6505
BsmI GAATG_Cn'
Cuts at: 2112 2988 4016 4109 2112
Size: 876 1028 93 4508
Fragments arranged by size:
4508 1028 876 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3323 3572 3323
Size: 249 6256
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2233 2513 2748 2796 3077 3297 3525
Size: 399 443 280 235 48 281 220 228
Cuts at: 3525 3552 3824 4566 4651 5722 1391
Size: 27 272 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 443 399 281 280 272
235 228 220 85 48 27
BspEI T'CCGG_A
Cuts at: 3015 6168 3015
Size: 3153 3352
BspHI T'CATG_A
Cuts at: 3456 4394 6124 3456
Size: 938 1730 3837
Fragments arranged by size:
3837 1730 938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2607 3121 3142 3548 3561 3860 844
Size: 1763 514 21 406 13 299 3489
Fragments arranged by size:
3489 1763 514 406 299 21 13
BsrBI CCG'CTC
Cuts at: 1394 1823 4392 1394
Size: 429 2569 3507
Fragments arranged by size:
3507 2569 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2073 3584 5000 5174 65
Size: 1183 825 1511 1416 174 1396
Fragments arranged by size:
1511 1416 1396 1183 825 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2036 3316 5149 5336 1700
Size: 110 226 1280 1833 187 2869
Fragments arranged by size:
2869 1833 1280 226 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6505
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6505
BssSI C'ACGA_G
Cuts at: 2681 4565 5577 2681
Size: 1884 1012 3609
Fragments arranged by size:
3609 1884 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3004 3409 3529 794
Size: 2210 405 120 3770
Fragments arranged by size:
3770 2210 405 120
BstBI TT'CG_AA
Cuts at: 5309 5309
Size: 6505
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6505
BstXI CCAn_nnnn'nTGG
Cuts at: 2314 3262 2314
Size: 948 5557
BstYI r'GATC_y
Cuts at: 1972 2871 3009 3090 3835 4202 4587 4604 6056
Size: 899 138 81 745 367 385 17 1452
Cuts at: 6056 6142 6154 6500 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 899 745 385 367 346 138
86 81 17 12
Bsu36I CC'TnA_GG
Cuts at: 3417 3417
Size: 6505
BtgI C'CryG_G
Cuts at: 513 1065 2688 2808 513
Size: 552 1623 120 4210
Fragments arranged by size:
4210 1623 552 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5766
Fragments arranged by size:
5766 683 56
BtsI GCAGTG_nn'
Cuts at: 2221 4021 4815 4835 2221
Size: 1800 794 20 3891
Fragments arranged by size:
3891 1800 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6505
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6470
DraI TTT'AAA
Cuts at: 3809 3809
Size: 6505
DraIII CAC_nnn'GTG
Cuts at: 3631 3631
Size: 6505
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3664 5512 809
Size: 507 2348 1848 1802
Fragments arranged by size:
2348 1848 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2038 2167 3082
Size: 54 1425 50 275 18 208 129 915
Cuts at: 3082 3318 3952 4841 6470 8
Size: 236 634 889 1629 43
Fragments arranged by size:
1629 1425 915 889 634 275 236 208
129 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2167 3952 1537
Size: 275 355 1785 4090
Fragments arranged by size:
4090 1785 355 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4435 1353
Size: 3082 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5092 5466 5612 387
Size: 1064 138 3503 374 146 1280
Fragments arranged by size:
3503 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2746 3295 2746
Size: 549 5956
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2227 2336 2468 2690 2780 2807 3158
Size: 393 595 109 132 222 90 27 351
Cuts at: 3158 3293 3407 3638 3662 3797 4568 5165 5952
Size: 135 114 231 24 135 771 597 787
Cuts at: 5952 6220 1239
Size: 268 1524
Fragments arranged by size:
1524 787 771 597 595 393 351 268
231 222 135 135 132 114 109 90
27 24
EcoNI CCTnn'n_nnAGG
Cuts at: 3677 3791 3677
Size: 114 6391
EcoO109I rG'GnC_Cy
Cuts at: 3700 3784 3796 3700
Size: 84 12 6409
Fragments arranged by size:
6409 84 12
EcoRI G'AATT_C
Cuts at: 3814 4192 3814
Size: 378 6127
FseI GG_CCGG'CC
Cuts at: 2040 2040
Size: 6505
FspI TGC'GCA
Cuts at: 5011 5011
Size: 6505
HaeII r_GCGC'y
Cuts at: 1678 2650 5652 1678
Size: 972 3002 2531
Fragments arranged by size:
3002 2531 972
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3149 3181 5146 5178 5220
Size: 32 135 32 1577 32 1965 32 42
Cuts at: 5220 5252 1373
Size: 32 2626
Fragments arranged by size:
2626 1965 1577 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3304 3849 4095 669
Size: 988 281 1366 545 246 3079
Fragments arranged by size:
3079 1366 988 545 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6505
HpaI GTT'AAC
Cuts at: 4095 4095
Size: 6505
KpnI G_GTAC'C
Cuts at: 3830 3830
Size: 6505
MfeI C'AATT_G
Cuts at: 4104 4104
Size: 6505
MluI A'CGCG_T
Cuts at: 6381 6381
Size: 6505
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5619 5803 1305
Size: 4314 184 2007
Fragments arranged by size:
4314 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4683
Fragments arranged by size:
4683 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2276 3555 3713 4463 4822 4981 518
Size: 648 1110 1279 158 750 359 159 2042
Fragments arranged by size:
2042 1279 1110 750 648 359 159 158
MspA1I CmG'CkG
Cuts at: 1606 3099 3106 3205 3529 3921 4598 5746 5991
Size: 1493 7 99 324 392 677 1148 245
Cuts at: 5991 1606
Size: 2120
Fragments arranged by size:
2120 1493 1148 677 392 324 245 99
7
NaeI GCC'GGC
Cuts at: 2038 3318 2038
Size: 1280 5225
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5953
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6505
NgoMIV G'CCGG_C
Cuts at: 2036 3316 2036
Size: 1280 5225
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6505
NotI GC'GGCC_GC
Cuts at: 3952 3952
Size: 6505
NspI r_CATG'y
Cuts at: 1132 1229 2902 3166 3316 3863 5408 1132
Size: 97 1673 264 150 547 1545 2229
Fragments arranged by size:
2229 1673 1545 547 264 150 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6505
PciI A'CATG_T
Cuts at: 2898 3162 5404 2898
Size: 264 2242 3999
Fragments arranged by size:
3999 2242 264
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2047 2479 1108
Size: 939 432 5134
Fragments arranged by size:
5134 939 432
PfoI T'CCnGG_A
Cuts at: 2184 2757 3642 3728 2184
Size: 573 885 86 4961
Fragments arranged by size:
4961 885 573 86
PmeI GTTT'AAAC
Cuts at: 3809 3809
Size: 6505
PmlI CAC'GTG
Cuts at: 2476 2476
Size: 6505
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4574 4606 6087 6119 4574
Size: 32 1481 32 4960
Fragments arranged by size:
4960 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3700 3700
Size: 6505
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6505
PsiI TTA'TAA
Cuts at: 2541 4075 2541
Size: 1534 4971
PstI C_TGCA'G
Cuts at: 830 3009 3857 830
Size: 2179 848 3478
Fragments arranged by size:
3478 2179 848
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6505
PvuII CAG'CTG
Cuts at: 3205 3529 3205
Size: 324 6181
SacI G_AGCT'C
Cuts at: 2748 3297 2748
Size: 549 5956
SalI G'TCGA_C
Cuts at: 1936 3847 1936
Size: 1911 4594
SbfI CC_TGCA'GG
Cuts at: 3857 3857
Size: 6505
ScaI AGT'ACT
Cuts at: 4753 6465 4753
Size: 1712 4793
SfcI C'TryA_G
Cuts at: 826 1045 3005 3595 3853 4988 5669 5860 826
Size: 219 1960 590 258 1135 681 191 1471
Fragments arranged by size:
1960 1471 1135 681 590 258 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6505
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6505
SmaI CCC'GGG
Cuts at: 1872 3832 1872
Size: 1960 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3113 3605 4609 5510 5772 820
Size: 197 926 1170 492 1004 901 262 1553
Fragments arranged by size:
1553 1170 1004 926 901 492 262 197
SnaBI TAC'GTA
Cuts at: 493 4296 493
Size: 3803 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6505
SphI G_CATG'C
Cuts at: 1132 3863 1132
Size: 2731 3774
SspI AAT'ATT
Cuts at: 5 52 4429 5
Size: 47 4377 2081
Fragments arranged by size:
4377 2081 47
StuI AGG'CCT
Cuts at: 1517 2459 2924 1517
Size: 942 465 5098
Fragments arranged by size:
5098 942 465
StyI C'CwwG_G
Cuts at: 513 1065 2028 3189 3711 3944 513
Size: 552 963 1161 522 233 3074
Fragments arranged by size:
3074 1161 963 552 522 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2145 2174 2306 2571 2834 2971 4532
Size: 1255 565 29 132 265 263 137 1561
Cuts at: 4532 4549 4702 4887 5332 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1561 1498 1255 565 445 265 263 185
153 137 132 29 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3159 4751 6463
Size: 274 80 33 51 1443 1182 1592 1712
Cuts at: 6463 96
Size: 138
Fragments arranged by size:
1712 1592 1443 1182 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6465 6497 6465
Size: 32 6473
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6505
XbaI T'CTAG_A
Cuts at: 3841 3841
Size: 6505
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3718 3718
Size: 6505
XhoI C'TCGA_G
Cuts at: 1943 3113 1943
Size: 1170 5335
XmaI C'CCGG_G
Cuts at: 1870 3830 1870
Size: 1960 4545
XmnI GAAnn'nnTTC
Cuts at: 4634 4634
Size: 6505
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4313 276
Size: 53 83 186 936 2779 2468
Fragments arranged by size:
2779 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbeI BfrBI BplI BstZ17I
EcoRV FalI FspAI KasI NarI NruI NsiI PacI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI