Restriction map for
pFN21AB8300
(Circular) (Six-base) MAPSORT of: pFN21AB8300.seq Check: 670 from: 1 to: 6094
pFN21AB8300 6094 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3259 3259
Size: 6094
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2751 3197 3904 278
Size: 53 83 186 936 1215 446 707 2468
Fragments arranged by size:
2468 1215 936 707 446 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3291 3437 1937
Size: 1354 146 4594
Fragments arranged by size:
4594 1354 146
Acc65I G'GTAC_C
Cuts at: 3415 3415
Size: 6094
AclI AA'CG_TT
Cuts at: 1563 4221 4594 1563
Size: 2658 373 3063
Fragments arranged by size:
3063 2658 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4157 5541 5809 1632
Size: 2525 1384 268 1917
Fragments arranged by size:
2525 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5897
AflIII A'CryG_T
Cuts at: 1203 4993 5970 1203
Size: 3790 977 1327
Fragments arranged by size:
3790 1327 977
AgeI A'CCGG_T
Cuts at: 4925 4925
Size: 6094
AhdI GACnn_n'nnGTC
Cuts at: 4823 4823
Size: 6094
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6094
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2850 1184
Size: 1666 4428
AlwNI CAG_nnn'CTG
Cuts at: 2284 3837 5409 2284
Size: 1553 1572 2969
Fragments arranged by size:
2969 1572 1553
ApaI G_GGCC'C
Cuts at: 2291 2950 2291
Size: 659 5435
ApaLI G'TGCA_C
Cuts at: 2720 4151 5307 2720
Size: 1431 1156 3507
Fragments arranged by size:
3507 1431 1156
ApoI r'AATT_y
Cuts at: 1493 3403 3629 3781 5890 1493
Size: 1910 226 152 2109 1697
Fragments arranged by size:
2109 1910 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4648 160
Size: 872 3616 1606
Fragments arranged by size:
3616 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6094
AvaI C'yCGr_G
Cuts at: 1870 1943 3419 1870
Size: 73 1476 4545
Fragments arranged by size:
4545 1476 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3249 3282 3249
Size: 33 6061
BamHI G'GATC_C
Cuts at: 3424 3791 3424
Size: 367 5727
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2072 2528 2965 3014 3415
Size: 325 217 627 285 456 437 49 401
Cuts at: 3415 4870 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 456 437 401 325 285
217 49
BanII G_rGCy'C
Cuts at: 1391 2291 2471 2663 2700 2950 3032 3413 1391
Size: 900 180 192 37 250 82 381 4072
Fragments arranged by size:
4072 900 381 250 192 180 82 37
BbeI G_GCGC'C
Cuts at: 2969 2969
Size: 6094
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2258 928
Size: 971 359 4764
Fragments arranged by size:
4764 971 359
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4285 4319 1903
Size: 34 2348 34 3678
Fragments arranged by size:
3678 2348 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3986 5196 3986
Size: 1210 4884
BclI T'GATC_A
Cuts at: 1548 2580 2976 1548
Size: 1032 396 4666
Fragments arranged by size:
4666 1032 396
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2216 2241 2843
Size: 107 122 71 631 605 544 25 602
Cuts at: 2843 3036 4705 136
Size: 193 1669 1525
Fragments arranged by size:
1669 1525 631 605 602 544 193 122
107 71 25
BglII A'GATC_T
Cuts at: 2607 6089 2607
Size: 3482 2612
BlpI GC'TnA_GC
Cuts at: 3511 3511
Size: 6094
Bme1580I G_kGCm'C
Cuts at: 1790 2077 2246 2291 2531 2687 2724 2950 3017
Size: 287 169 45 240 156 37 226 67
Cuts at: 3017 4155 5311 1790
Size: 1138 1156 2573
Fragments arranged by size:
2573 1156 1138 287 240 226 169 156
67 45 37
BmgBI CAC'GTC
Cuts at: 1318 2578 1318
Size: 1260 4834
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2105 3825 4783
Size: 416 53 83 399 24 688 1720 958
Cuts at: 4783 442
Size: 1753
Fragments arranged by size:
1753 1720 958 688 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6094
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2947 3334 2947
Size: 387 5707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2204 2357 2684 2904 2972 3209 4754 1239
Size: 965 153 327 220 68 237 1545 2579
Fragments arranged by size:
2579 1545 965 327 237 220 153 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2477 4219 5084 5382 2477
Size: 1742 865 298 3189
Fragments arranged by size:
3189 1742 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2504 3371 4757 882
Size: 617 1005 867 1386 2219
Fragments arranged by size:
2219 1386 1005 867 617
BsaAI yAC'GTr
Cuts at: 493 1204 3885 493
Size: 711 2681 2702
Fragments arranged by size:
2702 2681 711
BsaBI GATnn'nnATC
Cuts at: 2585 6088 2585
Size: 3503 2591
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2748 2966 3159 3194
Size: 53 83 186 936 1215 218 193 35
Cuts at: 3194 3348 3901 4283 4918 275
Size: 154 553 382 635 1451
Fragments arranged by size:
1451 1215 936 635 553 382 218 193
186 154 83 53 35
BsaWI w'CCGG_w
Cuts at: 3123 4527 4925 5199 5346 5757 3123
Size: 1404 398 274 147 411 3460
Fragments arranged by size:
3460 1404 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2795 2825 1178
Size: 30 165 30 1392 30 4447
Fragments arranged by size:
4447 1392 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2417 2873 1186
Size: 1231 456 4407
Fragments arranged by size:
4407 1231 456
BseYI C'CCAG_C
Cuts at: 5297 5793 5297
Size: 496 5598
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2699 2720 3287 1163
Size: 573 963 21 567 3970
Fragments arranged by size:
3970 963 573 567 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2215 3544 4305 5333 1540
Size: 275 186 214 1329 761 1028 2301
Fragments arranged by size:
2301 1329 1028 761 275 214 186
BsiHKAI G_wGCw'C
Cuts at: 2700 2724 4155 4240 5311 2700
Size: 24 1431 85 1071 3483
Fragments arranged by size:
3483 1431 1071 85 24
BsmI GAATG_Cn'
Cuts at: 3605 3698 3605
Size: 93 6001
BsmBI CGTCTCn'nnnn_
Cuts at: 2399 3079 3185 2399
Size: 680 106 5308
Fragments arranged by size:
5308 680 106
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2077 2246 2291 2471 2531 2663 2687
Size: 399 287 169 45 180 60 132 24
Cuts at: 2687 2700 2724 2950 3017 3032 3413 4155 4240
Size: 13 24 226 67 15 381 742 85
Cuts at: 4240 5311 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 381 287 226 180
169 132 85 67 60 45 24 24
15 13
BspEI T'CCGG_A
Cuts at: 5757 5757
Size: 6094
BspHI T'CATG_A
Cuts at: 3983 5713 3983
Size: 1730 4364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3060 3259 3449 844
Size: 2216 199 190 3489
Fragments arranged by size:
3489 2216 199 190
BsrBI CCG'CTC
Cuts at: 1394 1823 2625 3318 3981 1394
Size: 429 802 693 663 3507
Fragments arranged by size:
3507 802 693 663 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4589 4763 65
Size: 1183 3341 174 1396
Fragments arranged by size:
3341 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2210 2315 3060 4738 4925 1700
Size: 110 400 105 745 1678 187 2869
Fragments arranged by size:
2869 1678 745 400 187 110 105
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6094
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6094
BssSI C'ACGA_G
Cuts at: 2738 4154 5166 2738
Size: 1416 1012 3666
Fragments arranged by size:
3666 1416 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3126 794
Size: 2332 3762
BstBI TT'CG_AA
Cuts at: 4898 4898
Size: 6094
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6094
BstXI CCAn_nnnn'nTGG
Cuts at: 2266 2272 2506 2266
Size: 6 234 5854
Fragments arranged by size:
5854 234 6
BstYI r'GATC_y
Cuts at: 1972 2040 2436 2607 3111 3424 3791 4176 4193
Size: 68 396 171 504 313 367 385 17
Cuts at: 4193 5645 5731 5743 6089 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 504 396 385 367 346 313
171 86 68 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2265 2992 3381 513
Size: 552 939 261 727 389 3226
Fragments arranged by size:
3226 939 727 552 389 261
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2245 2296 487
Size: 683 56 1019 51 4285
Fragments arranged by size:
4285 1019 683 56 51
BtsI GCAGTG_nn'
Cuts at: 2326 2687 3197 3610 4404 4424 2326
Size: 361 510 413 794 20 3996
Fragments arranged by size:
3996 794 510 413 361 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6094
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6059
DraI TTT'AAA
Cuts at: 3398 3398
Size: 6094
DraIII CAC_nnn'GTG
Cuts at: 2728 2728
Size: 6094
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5101 809
Size: 507 3785 1802
Fragments arranged by size:
3785 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2233 2256 2982
Size: 54 1425 50 275 18 403 23 726
Cuts at: 2982 3541 4430 6059 8
Size: 559 889 1629 43
Fragments arranged by size:
1629 1425 889 726 559 403 275 54
50 43 23 18
EagI C'GGCC_G
Cuts at: 1537 1812 3541 1537
Size: 275 1729 4090
Fragments arranged by size:
4090 1729 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2789 3305 3362 4024 1353
Size: 1436 516 57 662 3423
Fragments arranged by size:
3423 1436 662 516 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2885 4681 5055 5201 387
Size: 1064 138 1296 1796 374 146 1280
Fragments arranged by size:
1796 1296 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2698 2698
Size: 6094
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2204 2357 2684 2904 2972 3209 4157
Size: 393 572 153 327 220 68 237 948
Cuts at: 4157 4754 5541 5809 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 948 787 597 572 393 327 268
237 220 153 68
EcoNI CCTnn'n_nnAGG
Cuts at: 2552 2552
Size: 6094
EcoO109I rG'GnC_Cy
Cuts at: 2281 2288 2947 2281
Size: 7 659 5428
Fragments arranged by size:
5428 659 7
EcoRI G'AATT_C
Cuts at: 3403 3781 3403
Size: 378 5716
FspI TGC'GCA
Cuts at: 4600 4600
Size: 6094
HaeII r_GCGC'y
Cuts at: 1678 2360 2783 2969 5241 1678
Size: 682 423 186 2272 2531
Fragments arranged by size:
2531 2272 682 423 186
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2197 2229 2795 2827 4735
Size: 32 135 32 625 32 566 32 1908
Cuts at: 4735 4767 4809 4841 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1908 625 566 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3438 3684 669
Size: 988 281 1500 246 3079
Fragments arranged by size:
3079 1500 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6094
HpaI GTT'AAC
Cuts at: 3684 3684
Size: 6094
KasI G'GCGC_C
Cuts at: 2965 2965
Size: 6094
KpnI G_GTAC'C
Cuts at: 3419 3419
Size: 6094
MfeI C'AATT_G
Cuts at: 3693 3693
Size: 6094
MluI A'CGCG_T
Cuts at: 5970 5970
Size: 6094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5208 5392 1305
Size: 3903 184 2007
Fragments arranged by size:
3903 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2235 2984 10
Size: 54 1425 343 403 749 3120
Fragments arranged by size:
3120 1425 749 403 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2264 2270 2504 2534 4052 4411 4570
Size: 648 1098 6 234 30 1518 359 159
Cuts at: 4570 518
Size: 2042
Fragments arranged by size:
2042 1518 1098 648 359 234 159 30
6
MspA1I CmG'CkG
Cuts at: 1606 2152 3227 3510 4187 5335 5580 1606
Size: 546 1075 283 677 1148 245 2120
Fragments arranged by size:
2120 1148 1075 677 546 283 245
NaeI GCC'GGC
Cuts at: 2317 2317
Size: 6094
NarI GG'CG_CC
Cuts at: 2966 2966
Size: 6094
NcoI C'CATG_G
Cuts at: 513 1065 2004 2265 3381 513
Size: 552 939 261 1116 3226
Fragments arranged by size:
3226 1116 939 552 261
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6094
NgoMIV G'CCGG_C
Cuts at: 2315 2315
Size: 6094
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6094
NotI GC'GGCC_GC
Cuts at: 3541 3541
Size: 6094
NspI r_CATG'y
Cuts at: 1132 1229 2914 3452 4997 1132
Size: 97 1685 538 1545 2229
Fragments arranged by size:
2229 1685 1545 538 97
PasI CC'CwG_GG
Cuts at: 1348 2284 2767 1348
Size: 936 483 4675
Fragments arranged by size:
4675 936 483
PciI A'CATG_T
Cuts at: 4993 4993
Size: 6094
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2914 1108
Size: 1806 4288
PfoI T'CCnGG_A
Cuts at: 2034 2193 3303 2034
Size: 159 1110 4825
Fragments arranged by size:
4825 1110 159
PmeI GTTT'AAAC
Cuts at: 3398 3398
Size: 6094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4163 4195 5676 5708 4163
Size: 32 1481 32 4549
Fragments arranged by size:
4549 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6094
PsiI TTA'TAA
Cuts at: 3664 3664
Size: 6094
PspOMI G'GGCC_C
Cuts at: 2287 2946 2287
Size: 659 5435
PstI C_TGCA'G
Cuts at: 830 3446 830
Size: 2616 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6094
PvuII CAG'CTG
Cuts at: 2152 3227 2152
Size: 1075 5019
SacI G_AGCT'C
Cuts at: 2700 2700
Size: 6094
SalI G'TCGA_C
Cuts at: 1936 3436 1936
Size: 1500 4594
SapI GCTCTTCn'nnn_
Cuts at: 2789 2789
Size: 6094
SbfI CC_TGCA'GG
Cuts at: 3446 3446
Size: 6094
ScaI AGT'ACT
Cuts at: 4342 6054 4342
Size: 1712 4382
SfcI C'TryA_G
Cuts at: 826 1045 3088 3442 4577 5258 5449 826
Size: 219 2043 354 1135 681 191 1471
Fragments arranged by size:
2043 1471 1135 681 354 219 191
SfoI GGC'GCC
Cuts at: 2967 2967
Size: 6094
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6094
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6094
SmaI CCC'GGG
Cuts at: 1872 3421 1872
Size: 1549 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2492 4198 5099 5361 820
Size: 197 926 549 1706 901 262 1553
Fragments arranged by size:
1706 1553 926 901 549 262 197
SnaBI TAC'GTA
Cuts at: 493 3885 493
Size: 3392 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6094
SphI G_CATG'C
Cuts at: 1132 3452 1132
Size: 2320 3774
SspI AAT'ATT
Cuts at: 5 52 4018 5
Size: 47 3966 2081
Fragments arranged by size:
3966 2081 47
StuI AGG'CCT
Cuts at: 1517 2369 1517
Size: 852 5242
StyI C'CwwG_G
Cuts at: 513 1065 2004 2112 2265 2472 2553 2838 3381
Size: 552 939 108 153 207 81 285 543
Cuts at: 3381 3533 513
Size: 152 3074
Fragments arranged by size:
3074 939 552 543 285 207 153 152
108 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2366 2780 4121 4138 4291 4476 4921
Size: 1255 786 414 1341 17 153 185 445
Cuts at: 4921 325
Size: 1498
Fragments arranged by size:
1498 1341 1255 786 445 414 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3327 4340 6052
Size: 274 80 33 51 1443 1350 1013 1712
Cuts at: 6052 96
Size: 138
Fragments arranged by size:
1712 1443 1350 1013 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2714 2746 6054 6086 2714
Size: 32 3308 32 2722
Fragments arranged by size:
3308 2722 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2561 2903 1316
Size: 1245 342 4507
Fragments arranged by size:
4507 1245 342
XbaI T'CTAG_A
Cuts at: 3430 3430
Size: 6094
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2521 3052 2521
Size: 531 5563
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6094
XmaI C'CCGG_G
Cuts at: 1870 3419 1870
Size: 1549 4545
XmnI GAAnn'nnTTC
Cuts at: 4223 4223
Size: 6094
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2749 3195 3902 276
Size: 53 83 186 936 1215 446 707 2468
Fragments arranged by size:
2468 1215 936 707 446 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbvCI BfrBI Bpu10I BsiWI
BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SrfI SwaI