Restriction map for
pFN21AB8296
(Circular) (Six-base) MAPSORT of: pFN21AB8296.seq Check: 1932 from: 1 to: 5971
pFN21AB8296 5971 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3781 278
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3314 1937
Size: 1377 4594
Acc65I G'GTAC_C
Cuts at: 3292 3292
Size: 5971
AclI AA'CG_TT
Cuts at: 1563 4098 4471 1563
Size: 2535 373 3063
Fragments arranged by size:
3063 2535 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2388 2978 4034 5418 5686 1632
Size: 756 590 1056 1384 268 1917
Fragments arranged by size:
1917 1384 1056 756 590 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5774
AflIII A'CryG_T
Cuts at: 1203 4870 5847 1203
Size: 3667 977 1327
Fragments arranged by size:
3667 1327 977
AgeI A'CCGG_T
Cuts at: 4802 4802
Size: 5971
AhdI GACnn_n'nnGTC
Cuts at: 4700 4700
Size: 5971
AleI CACnn'nnGTG
Cuts at: 1166 3258 1166
Size: 2092 3879
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5971
AlwNI CAG_nnn'CTG
Cuts at: 3714 5286 3714
Size: 1572 4399
ApaI G_GGCC'C
Cuts at: 2768 2768
Size: 5971
ApaLI G'TGCA_C
Cuts at: 4028 5184 4028
Size: 1156 4815
ApoI r'AATT_y
Cuts at: 1493 2967 3280 3506 3658 5767 1493
Size: 1474 313 226 152 2109 1697
Fragments arranged by size:
2109 1697 1474 313 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4525 160
Size: 872 3493 1606
Fragments arranged by size:
3493 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
AvaI C'yCGr_G
Cuts at: 1870 1943 2041 3219 3296 1870
Size: 73 98 1178 77 4545
Fragments arranged by size:
4545 1178 98 77 73
BamHI G'GATC_C
Cuts at: 3301 3668 3301
Size: 367 5604
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2188 2428 3228 3292 4747
Size: 325 217 627 401 240 800 64 1455
Cuts at: 4747 618
Size: 1842
Fragments arranged by size:
1842 1455 800 627 401 325 240 217
64
BanII G_rGCy'C
Cuts at: 1391 2396 2768 2859 3126 3290 1391
Size: 1005 372 91 267 164 4072
Fragments arranged by size:
4072 1005 372 267 164 91
BbeI G_GCGC'C
Cuts at: 2432 3232 2432
Size: 800 5171
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2202 2586 928
Size: 971 303 384 4313
Fragments arranged by size:
4313 971 384 303
BbvCI CC'TCA_GC
Cuts at: 2690 2690
Size: 5971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4162 4196 1903
Size: 34 2225 34 3678
Fragments arranged by size:
3678 2225 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3863 5073 3863
Size: 1210 4761
BclI T'GATC_A
Cuts at: 1548 3016 1548
Size: 1468 4503
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4582 136
Size: 107 122 71 631 605 2910 1525
Fragments arranged by size:
2910 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5966 5966
Size: 5971
BlpI GC'TnA_GC
Cuts at: 2058 3388 2058
Size: 1330 4641
Bme1580I G_kGCm'C
Cuts at: 1790 2768 2982 4032 5188 1790
Size: 978 214 1050 1156 2573
Fragments arranged by size:
2573 1156 1050 978 214
BmgBI CAC'GTC
Cuts at: 1318 2599 1318
Size: 1281 4690
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3702 4660 442
Size: 416 53 83 399 24 2285 958 1753
Fragments arranged by size:
2285 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3110 3231 4631 1239
Size: 1871 121 1400 2579
Fragments arranged by size:
2579 1871 1400 121
Bpu10I CC'TnA_GC
Cuts at: 2547 2690 2734 2547
Size: 143 44 5784
Fragments arranged by size:
5784 143 44
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2325 2891 4096 4961 5259 2325
Size: 566 1205 865 298 3037
Fragments arranged by size:
3037 1205 865 566 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2324 4634 882
Size: 617 825 2310 2219
Fragments arranged by size:
2310 2219 825 617
BsaAI yAC'GTr
Cuts at: 493 1204 3762 493
Size: 711 2558 2702
Fragments arranged by size:
2702 2558 711
BsaBI GATnn'nnATC
Cuts at: 5965 5965
Size: 5971
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2429 3229 3267 3778
Size: 53 83 186 936 896 800 38 511
Cuts at: 3778 4160 4795 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 896 800 635 511 382 186
83 53 38
BsaWI w'CCGG_w
Cuts at: 4404 4802 5076 5223 5634 4404
Size: 398 274 147 411 4741
Fragments arranged by size:
4741 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5746
Fragments arranged by size:
5746 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2369 2792 1186
Size: 1183 423 4365
Fragments arranged by size:
4365 1183 423
BseYI C'CCAG_C
Cuts at: 2560 2605 5174 5670 2560
Size: 45 2569 496 2861
Fragments arranged by size:
2861 2569 496 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5398
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3421 4182 5210 1540
Size: 275 186 1420 761 1028 2301
Fragments arranged by size:
2301 1420 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2466 3147 4032 4117 5188 2466
Size: 681 885 85 1071 3249
Fragments arranged by size:
3249 1071 885 681 85
BsmI GAATG_Cn'
Cuts at: 3482 3575 3482
Size: 93 5878
BsmBI CGTCTCn'nnnn_
Cuts at: 3096 3096
Size: 5971
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2396 2466 2768 2859 2982 3126 3147
Size: 399 606 70 302 91 123 144 21
Cuts at: 3147 3290 4032 4117 5188 1391
Size: 143 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 606 399 302 144 143
123 91 85 70 21
BspEI T'CCGG_A
Cuts at: 5634 5634
Size: 5971
BspHI T'CATG_A
Cuts at: 3860 5590 3860
Size: 1730 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3326 844
Size: 2482 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3858 1394
Size: 429 2035 3507
Fragments arranged by size:
3507 2035 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3180 4466 4640 65
Size: 1183 1932 1286 174 1396
Fragments arranged by size:
1932 1396 1286 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2362 4615 4802 1700
Size: 110 552 2253 187 2869
Fragments arranged by size:
2869 2253 552 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5971
BssHII G'CGCG_C
Cuts at: 1925 2035 1925
Size: 110 5861
BssSI C'ACGA_G
Cuts at: 2696 4031 5043 2696
Size: 1335 1012 3624
Fragments arranged by size:
3624 1335 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2986 794
Size: 2192 3779
BstBI TT'CG_AA
Cuts at: 4775 4775
Size: 5971
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5971
BstYI r'GATC_y
Cuts at: 1972 3114 3301 3668 4053 4070 5522 5608 5620
Size: 1142 187 367 385 17 1452 86 12
Cuts at: 5620 5966 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1142 385 367 346 187 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2769 2769
Size: 5971
BtgI C'CryG_G
Cuts at: 513 1065 2004 2052 513
Size: 552 939 48 4432
Fragments arranged by size:
4432 939 552 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5232
Fragments arranged by size:
5232 683 56
BtsI GCAGTG_nn'
Cuts at: 3253 3265 3487 4281 4301 3253
Size: 12 222 794 20 4923
Fragments arranged by size:
4923 794 222 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5971
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2434 2469 529
Size: 35 1870 35 4031
Fragments arranged by size:
4031 1870 35 35
DraI TTT'AAA
Cuts at: 3275 3275
Size: 5971
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2840 4978 809
Size: 507 1524 2138 1802
Fragments arranged by size:
2138 1802 1524 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2640 2787 3418
Size: 54 1425 50 275 18 810 147 631
Cuts at: 3418 4307 5936 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 810 631 275 147 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3418 1537
Size: 275 1606 4090
Fragments arranged by size:
4090 1606 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2913 3901 1353
Size: 1560 988 3423
Fragments arranged by size:
3423 1560 988
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3155 4558 4932 5078 387
Size: 1064 138 1566 1403 374 146 1280
Fragments arranged by size:
1566 1403 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2388 2978 3110 3231 4034 4631 5418
Size: 393 756 590 132 121 803 597 787
Cuts at: 5418 5686 1239
Size: 268 1524
Fragments arranged by size:
1524 803 787 756 597 590 393 268
132 121
EcoNI CCTnn'n_nnAGG
Cuts at: 2909 2909
Size: 5971
EcoO109I rG'GnC_Cy
Cuts at: 2020 2066 2254 2765 2020
Size: 46 188 511 5226
Fragments arranged by size:
5226 511 188 46
EcoRI G'AATT_C
Cuts at: 2967 3280 3658 2967
Size: 313 378 5280
Fragments arranged by size:
5280 378 313
EcoRV GAT'ATC
Cuts at: 2614 2614
Size: 5971
FspI TGC'GCA
Cuts at: 4477 4477
Size: 5971
HaeII r_GCGC'y
Cuts at: 1678 2222 2432 3232 5118 1678
Size: 544 210 800 1886 2531
Fragments arranged by size:
2531 1886 800 544 210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2068 2100 2323 2355 2508
Size: 32 135 32 496 32 223 32 153
Cuts at: 2508 2540 4612 4644 4686 4718 1373
Size: 32 2072 32 42 32 2626
Fragments arranged by size:
2626 2072 496 223 153 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3315 3561 669
Size: 988 281 1377 246 3079
Fragments arranged by size:
3079 1377 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5971
HpaI GTT'AAC
Cuts at: 3561 3561
Size: 5971
KasI G'GCGC_C
Cuts at: 2428 3228 2428
Size: 800 5171
KpnI G_GTAC'C
Cuts at: 3296 3296
Size: 5971
MfeI C'AATT_G
Cuts at: 3570 3570
Size: 5971
MluI A'CGCG_T
Cuts at: 5847 5847
Size: 5971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5085 5269 1305
Size: 3780 184 2007
Fragments arranged by size:
3780 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4149
Fragments arranged by size:
4149 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3258 3929 4288 4447 518
Size: 648 2092 671 359 159 2042
Fragments arranged by size:
2092 2042 671 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2609 2879 3387 4064 5212 5457 1606
Size: 1003 270 508 677 1148 245 2120
Fragments arranged by size:
2120 1148 1003 677 508 270 245
NaeI GCC'GGC
Cuts at: 2364 2364
Size: 5971
NarI GG'CG_CC
Cuts at: 2429 3229 2429
Size: 800 5171
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4480
Fragments arranged by size:
4480 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5971
NgoMIV G'CCGG_C
Cuts at: 2362 2362
Size: 5971
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5971
NotI GC'GGCC_GC
Cuts at: 3418 3418
Size: 5971
NspI r_CATG'y
Cuts at: 1132 1229 3329 4874 1132
Size: 97 2100 1545 2229
Fragments arranged by size:
2229 2100 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5971
PciI A'CATG_T
Cuts at: 4870 4870
Size: 5971
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5971
PmeI GTTT'AAAC
Cuts at: 3275 3275
Size: 5971
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4040 4072 5553 5585 4040
Size: 32 1481 32 4426
Fragments arranged by size:
4426 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2020 2066 2020
Size: 46 5925
PshAI GACnn'nnGTC
Cuts at: 1613 2879 1613
Size: 1266 4705
PsiI TTA'TAA
Cuts at: 3541 3541
Size: 5971
PspOMI G'GGCC_C
Cuts at: 2764 2764
Size: 5971
PstI C_TGCA'G
Cuts at: 830 3260 3323 830
Size: 2430 63 3478
Fragments arranged by size:
3478 2430 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5971
SalI G'TCGA_C
Cuts at: 1936 3313 1936
Size: 1377 4594
SapI GCTCTTCn'nnn_
Cuts at: 2913 2913
Size: 5971
SbfI CC_TGCA'GG
Cuts at: 3323 3323
Size: 5971
ScaI AGT'ACT
Cuts at: 4219 5931 4219
Size: 1712 4259
SfcI C'TryA_G
Cuts at: 826 1045 2869 3256 3319 4454 5135 5326 826
Size: 219 1824 387 63 1135 681 191 1471
Fragments arranged by size:
1824 1471 1135 681 387 219 191 63
SfoI GGC'GCC
Cuts at: 2430 3230 2430
Size: 800 5171
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5971
SmaI CCC'GGG
Cuts at: 1872 3298 1872
Size: 1426 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2340 2906 4075 4976 5238 820
Size: 197 926 397 566 1169 901 262 1553
Fragments arranged by size:
1553 1169 926 901 566 397 262 197
SnaBI TAC'GTA
Cuts at: 493 3762 493
Size: 3269 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5971
SphI G_CATG'C
Cuts at: 1132 3329 1132
Size: 2197 3774
SspI AAT'ATT
Cuts at: 5 52 3895 5
Size: 47 3843 2081
Fragments arranged by size:
3843 2081 47
StuI AGG'CCT
Cuts at: 1517 2711 1517
Size: 1194 4777
StyI C'CwwG_G
Cuts at: 513 1065 2004 2445 2481 2739 3118 3410 513
Size: 552 939 441 36 258 379 292 3074
Fragments arranged by size:
3074 939 552 441 379 292 258 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2584 3998 4015 4168 4353 4798 325
Size: 1255 1004 1414 17 153 185 445 1498
Fragments arranged by size:
1498 1414 1255 1004 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4217 5929 96
Size: 274 80 33 51 1443 2240 1712 138
Fragments arranged by size:
2240 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2482 2514 5931 5963 2482
Size: 32 3417 32 2490
Fragments arranged by size:
3417 2490 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5971
XbaI T'CTAG_A
Cuts at: 3307 3307
Size: 5971
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5971
XmaI C'CCGG_G
Cuts at: 1870 3296 1870
Size: 1426 4545
XmnI GAAnn'nnTTC
Cuts at: 2931 4100 2931
Size: 1169 4802
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3779 276
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstXI BstZ17I DraIII EcoICRI FalI FseI FspAI
NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI
XcmI